protein engineering
DESCRIPTION
Protein structure computational design and engineering. Application to proteins, enzymes, peptidomimetics, peptides, vaccines, antibodiesTRANSCRIPT
Structure-based Protein Design and Engineering
• What can it do for you?
• How it works?
Health, Environment and Technology
Molecular Modelling Mini Tutorial
Protein-based Technologies are widely used in our daily life
Health Environment Technology
Molecular Modelling Mini Tutorial
Proteins Enzymes and Peptides are made of a sequence of aminoacids
1 MASPFALLMV LVVLSCKSSC SLGCDLPETH SLDNRRTLML LAQMSRISPS SCLMDRHDFG 61 FPQEEFDGNQ FQKAPAISVL HELIQQIFNL FTTKDSSAAW DEDLLDKFCT ELYQQLNDLE121 ACVMQEERVG ETPLMNADSI LAVKKYFRRI TLYLTEKKYS PCAWEVVRAE IMRSLSLSTN181 LQERLRRKE
This is the sequence of a well known protein.
Can you guess what it is, what it does and how it performs it function?
Molecular Modelling Mini Tutorial
Protein function can be understoodby its 3D molecular structure
Protein structures can be determined by:
X-ray NMR Homology Modelling
Molecular Modelling Mini Tutorial
Do you have a sequence and want to …
• Know it’s structure
• Understand it’s function
• Improve it’s native properties
• Perform targeted mutations
• Improve structure specific features
Molecular Modelling Mini Tutorial
Bimolecular Modelling can help you!
Active site Protein-proteinaggregation site
Conserved domain
Mobile domainHydrophobicpatch
We model a 3D structure
from your sequence, and provide
detailed structural and dynamic
insights concerning it’s function
Molecular Modelling Mini Tutorial
What about engineering improvedprotein function and structure?
Focus your effort on the most
promising recombinant proteins
predicted by our computer-aided
protein structure design platform
We can perform large scale
aminoacid mutation screening,
so you can focus your effort on
the most promising
recombinant proteins.
Molecular Modelling Mini Tutorial
Molecular Modelling can support your research & development effort on:
• Vaccines
• Antibodies
• Peptides
• Peptidomimetics
• Enzymes
• Other protein-based technologies
Molecular Modelling Mini Tutorial
Dr. Nuno MicaeloResearcher, Aveiro University