propolis – a nature alternative by vaginal disturbances?
DESCRIPTION
Therapy - Intention to reestablish a physiological situation of the vagina. How does the optimal therapy look? Reduces the sensible quantity of germs AND represses the subjective disturbances. General antimicrobial (Bacteria, Fungal, Virus). Vaginal equilibrium is established and keep alive itself. Shouldn’t cause Resistance and should be applicable as Prophylaxes. Antibacterial mechanism of action of Propolis: DNA dependant on RNA Polymerase as follows: Bacteria are spited slowly Penetrability of cell membrane increased as follows: Bacteria get more sensitiv. The Hydroxy-cinnamon-acid inhibits reversible the Motility of Bacteria s follows: slow dissermination Visit our Website: www.imi.co.atTRANSCRIPT
Propolis – a nature alternative by vaginal disturbances?
Martin Imhof MD, PhDMartin Imhof MD, PhD
University Hospital for Gynecology ViennaUniversity Hospital for Gynecology Vienna
Dep. of Endocrinology and Treatment of Dep. of Endocrinology and Treatment of Fertility Fertility
Vaginal Infektion
InzidenceInzidence
70-75% of all women 1 x in live70-75% of all women 1 x in live
40-50% suffer from reoccuring Infection 40-50% suffer from reoccuring Infection (=> 4 Infections / year)(=> 4 Infections / year)
Vaginal Complaints
germ germ = Primary cause for = Primary cause for complainscomplains
cause cause = Failure of defense system = Failure of defense system
Therapy - Intention
… … to reestablish a physiologicalto reestablish a physiological
situation of the vagina situation of the vagina
Problem of AB Therapy
Selection of a pathological germ-groupSelection of a pathological germ-group
Good case
Physiological equilibrium
is reached again
Worst case
another pathogenic
germ renews the
Infection => Relapse
1-6 weeks after Therapy
Antibiotika und Antimykotika
Disadvantage Disadvantage
Adverse effects (for ins.: digestion)Adverse effects (for ins.: digestion)
Resistance of treated germsResistance of treated germs
barely prophylactic applicable!barely prophylactic applicable!
How does the optimal therapy look?
Reduces the sensible quantity of germs AND Reduces the sensible quantity of germs AND represses the subjective disturbancesrepresses the subjective disturbances
General antimicrobial (Bacteria, Fungal, Virus)General antimicrobial (Bacteria, Fungal, Virus)
Vaginal equilibrium is established and keep Vaginal equilibrium is established and keep alive itselfalive itself
ShouldnShouldn’’t cause Resistance and should be t cause Resistance and should be applicable as Prophylaxesapplicable as Prophylaxes
P R O P O L I S ?P R O P O L I S ?
Propolis
Makes the Bee-yard „aseptic“ Makes the Bee-yard „aseptic“
““Cement” and “Carpet of epidemic” Cement” and “Carpet of epidemic”
Antibacterial mechanism of action of Propolis
DNA dependant on RNA Polymerase DNA dependant on RNA Polymerase as follows: Bacteria are spited slowlyas follows: Bacteria are spited slowly
Penetrability of cell membrane increased Penetrability of cell membrane increased as follows: Bacteria get more sensitiv. as follows: Bacteria get more sensitiv.
The Hydroxy-cinnamon-acid inhibits The Hydroxy-cinnamon-acid inhibits reversible the Motility of Bacteria reversible the Motility of Bacteria as follows: slow dissermination as follows: slow dissermination
Anti-fungal effect of Propolis
Propolis in the Concentration of 0,25% to 2% is Propolis in the Concentration of 0,25% to 2% is effective against div. Dermal-phyteneffective against div. Dermal-phyten
Flavenoide legates Enzymes und Metal-ions Flavenoide legates Enzymes und Metal-ions which is important for the fungal-metabolism which is important for the fungal-metabolism
Antiviral Effect of Propolis
Is affected by different Virus (Influenza, Is affected by different Virus (Influenza, Herpes, Polio, Hepatitis as well as felines Herpes, Polio, Hepatitis as well as felines HIV)HIV)
viral DNA Production viral DNA Production in vitro is in vitro is inhibited to inhibited to factor 32. factor 32.
Inhibits the viral Adsorption at the cells Inhibits the viral Adsorption at the cells surface areasurface area
Effectively of Propolis against Protozoa
typical Parasite: typical Parasite: Trichomonas vaginalisTrichomonas vaginalis..
Propolis in the Concentration of 150 µg/ml Propolis in the Concentration of 150 µg/ml
shows a significant Inhibition of shows a significant Inhibition of trichomonal Proliferation.trichomonal Proliferation.
Antibacterial effectively of Propolis
010
2030
4050
6070
8090
100
0 200 400 600 800
concentration [µg/ml]
colo
ny fo
rmin
g un
it [%
of c
ontr
ol]
6 h B.s.
18 h B.s.
18 h E.coli
Antibacterial effects of Propolis on colony formation by Bacillus subtilis (Gram +) and Escherichia coli (Gram -) suspensions incubated on agar for 6 and 18 hours
In vitro Survival rate of Trichomonas vaginalis in different Concentration of
Propolis
0
10
20
30
40
50
60
70
80
90
100
0 50 100 150 200 250
t ime [h]
surv
ival
rat
e [%
]
control
150 µg/ml
50 µg/ml
5 µg/ml
0.5 µg/ml
0.05 µg/ml
The local anesthetic Propolis effect
„ „ ....... by subcutaneous application, the ....... by subcutaneous application, the Pinocembrin starts developing and the Café Pinocembrin starts developing and the Café acid-rest has almost the same anesthetic acid-rest has almost the same anesthetic effect than Lidocaineeffect than Lidocaine““
Paintz M, Metzner J.Paintz M, Metzner J.Pharmazie 1979 Dec;34(12):839-41 Pharmazie 1979 Dec;34(12):839-41
Propolis-therapy
Reduction of the total germ-exposureReduction of the total germ-exposure
RELATIVE physiologic gap by Lactobacillus RELATIVE physiologic gap by Lactobacillus acidophilusacidophilus
Reestablishment of physiologic situation Reestablishment of physiologic situation
Propolis-therapy
Rapid abatement of disorders through local-Rapid abatement of disorders through local-anesthetic effect (reinforcement)anesthetic effect (reinforcement)
No resistence due to biologic VariationNo resistence due to biologic Variation
Low side effects Low side effects
Propolis as a Therapy of vaginal disorders
M ImhofM Imhof
Universitätsklinik für Frauenheilkunde Universitätsklinik für Frauenheilkunde Wien Wien
melprotect®
Propolis (5%) camomile Lime-tree blossom pH 4.5
Study Propolis (melprotect®) -Irrigation
56 symptomatic Patients with reoccuring 56 symptomatic Patients with reoccuring Vaginal-Infection (>=4/year)Vaginal-Infection (>=4/year)
Anonymised questionnaires for Evaluation Anonymised questionnaires for Evaluation 2 weeks before and after the closing of 2 weeks before and after the closing of studies. studies.
After 6 months telephonic questionnaires After 6 months telephonic questionnaires
Using of MELPROTECT
1 x daily 30 ml per Applicator for one week1 x daily 30 ml per Applicator for one week
After acute therapy reduce melprotect slowlyAfter acute therapy reduce melprotect slowly
(every 2 day, every 3 day etc.)(every 2 day, every 3 day etc.)
Questionnaire 1. How strong have been/are the vaginal pains before/after the 1. How strong have been/are the vaginal pains before/after the
therapy?therapy? 2. How strong was/is the itching before/after the therapy?2. How strong was/is the itching before/after the therapy? 3. How strong was the cauterization before/after the therapy?3. How strong was the cauterization before/after the therapy? 4. How strong was/is the vaginal defluxion in the last month/after the 4. How strong was/is the vaginal defluxion in the last month/after the
therapy?therapy? 5. How hindering was/is the Vaginal-Infection before/after the therapy 5. How hindering was/is the Vaginal-Infection before/after the therapy
during the sexual intercourse?during the sexual intercourse? 6. How hindering was/is the eventual Vaginal-Infection before/after the 6. How hindering was/is the eventual Vaginal-Infection before/after the
therapy during normal sportive activities?therapy during normal sportive activities? 7. How hindering was/is the Vaginal-Infection in the last month / after 7. How hindering was/is the Vaginal-Infection in the last month / after
the therapy during the daily life activities the therapy during the daily life activities (shopping, going out,...) ?(shopping, going out,...) ?
8. How satisfied have you been/are you with the previous/actual 8. How satisfied have you been/are you with the previous/actual medicinal therapy for the Vaginal-Infection?medicinal therapy for the Vaginal-Infection?
Questionnaire - Analyse
0
1
2
3
4
5
6
7
8
Vorher 1,54 3,77 2,84 4,88 3,68 1,36 1,25 2,91
Nachher 0,13 1,09 0,93 2,09 0,79 0,11 0,38 7,36
Frage1 Frage2 Frage3 Frage4 Frage5 Frage6 Frage7 Frage8
Result 49 (87%) of patients described a reduction of disorders 49 (87%) of patients described a reduction of disorders
after the using of Propolis (melprotect)after the using of Propolis (melprotect)
7 (13%) of patients couldn’t notice a change of disorders 7 (13%) of patients couldn’t notice a change of disorders after the using of Propolis (melprotect)after the using of Propolis (melprotect)
0
5
10
15
20
25
Abstrichauswertung vor Therapie
Soorinfektion
E1
Mischinfektion
Gardnerella
Abstrich o.B.
0
10
20
30
40
50
Abstrichauswertung nach Therapie
Soorinfektion
E1
Mischinfektion
Gardnerella
Abstrich o.B.
Telephonic Questionnaireafter 6 Month
Following Question was asked:Following Question was asked:
1. Did you suffer on a new infection?1. Did you suffer on a new infection?
2. Did you get a therapy for the infected 2. Did you get a therapy for the infected disorders again?disorders again?
3. How do you judge the therapy-success?3. How do you judge the therapy-success?
Telephonic - Questionnaire Results
Patient, where an positive result was seen: Patient, where an positive result was seen:
31 (63%) of patients report no reinfection31 (63%) of patients report no reinfection
16 (33%) of patients report about the recurrence of 16 (33%) of patients report about the recurrence of SymptomsSymptoms
2 (4%) there was no questionnaire possible2 (4%) there was no questionnaire possible
* * ** * *Imhof M, Lipovac M, Kurz Ch, Barta J, Wiesenthal B, Huber J 5% propolis solution for the treatment of chronic vaginitis Int J Gynaecol Obstet. 2005 May;89(2):127-32.
Conclusion Antimicrobial effect against common germs Antimicrobial effect against common germs
No resistance similar to a Antibiotic therapyNo resistance similar to a Antibiotic therapy
Relative support of the physiological Vagina-floraRelative support of the physiological Vagina-flora
Local-anaesthetic effectLocal-anaesthetic effect
Allergic reaction very seldom Allergic reaction very seldom
The use for Gardnarella- und Chlamydien-The use for Gardnarella- und Chlamydien-infection is not assured infection is not assured
* * ** * *
Thank you very much Thank you very much for yourfor your
attention attention
Epicutaneous Test
30 patients30 patients melprotectmelprotect®® was applied in a test chamber was applied in a test chamber
on the skin for 48hrson the skin for 48hrs evaluation after 42hrs and 78hrsevaluation after 42hrs and 78hrs no allergenicity in any patientno allergenicity in any patient no irritation in any patientno irritation in any patient