progress in perennial rice breeding and genetics...o.eichingeri peter 24 cc sri lanka,africa...
TRANSCRIPT
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Progress in Perennial Rice
Breeding and Genetics
Fengyi Hu
Food Crops Research Institute,YAAS
22 Sept. WaggaWagga, Australia
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Introduction• Soil erosion in uplands of
southeast Asia has been a serious problem that led to the project of developing perennial upland rice at IRRI (IRRI 1989)
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The idea of developing perennial rice
for erosion control
Development of
perennial upland rice
has been proposed by
several authors (IRRI
1989; Wagoner, 1990;
Xiu, 1995; Schmit,
1996; Tao et al., 2000,
2001; Sacks, 2001;)
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Cultivars of rice is usual annual food crop after long time domestication by farmer and breeding procedure by breeder or geneticist.
The donor(s) for prerenniality?
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The donor(s) for prerenniality?
All over the world growth of O. sativa is Annual
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The donor(s) for prerenniality?
• O. longistaminata is the logical donor for
perenniality from its feature of rhizome as
compared to other wild rice species (O. officinalis,
O. rhizomatious, O. australiensis)
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Oryza Species, the Species Complex, Chrom.,Genome group and
Distribution
Oryza species, the species complexes, chromosome number, genome group and distribution
Section Chromosome Genome Distribution
Complex Number group
Species
Oryza
O.sativa
complex O.sativa L. 24 AA Worldwide
O.nivara Sharma et Shastry 24 AA Tropical and Sub.Asia
O.rufipogon Griff 24 AA Tropical and Sub.Asia
O.meridionalis Ng 24 Am
Am
Tropical Australia
O.glumaepatula Steud. 24 Agl
Agl
South America
O.glaberrima Steud. 24 AgA
g Africa(mainly West)
O.barthii A.Chev. 24 AgA
g Africa
O.longistaminata Chev.et Roehr 24 AlA
l Africa
O.officinalis
complex O.officinalis Wall ex Watt 24 CC Tropical and Sub.Asia
O.minuta Presl.et Presl. 48 BBCC Philippines
O.eichingeri Peter 24 CC Sri Lanka,Africa
O.rhizomatis Vaughan 24 CC Sri Lanka
O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa
O.latifolia Desv. 48 CCDD Latin America
O.alta Swallen 48 CCDD Latin America
O.grandiglumis (Doell) Prod. 48 CCDD South America
O.australiensis Domin 24 EE Australia
Ridleyanae
Tateoka O.brachyantha Chev.et Roehr. 24 FF Africa
O.schlechteri Pilger 48 HHKK Papua New Guinea
O.ridleyi
complex O.ridleyi Hook.f. 48 HHJJ SE Asia
O.longiglumis Jansen 48 HHJJ Irian Jaya,Indonesia
Granulata O.meyeriana
Roschev. complex O.meyeriana Baill 24 GG SE Asia
O.granulata Nees et Arn.ex Watt 24 GG S.and SE Asia
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The Diagram of Evolution of Wild Species among AA Genome of Rice
Gondawanaland Common ancestor
South and Southeast Asia Tropical Africa
O. rufipogon O . longistaminata
O. nivar O. barthii
Indica ---- Japonica O . glaberrima
Parallel evolution
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Comparing the O. longistaminata and O. rufipogon
O. longistaminata (AA genome)
Photo was cited from Vaughan (1994)
O. rufipogon (AA genome)
Photo was cited from Vaughan (1994)
Tufted and scrambling herb, stolon
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O. longistaminata
• Long anther
• Self-
incompatibility
• Allogamy
• Rhizomatous
stem
• Bacterial Blight
resistance(Xa21)
• Nematode
resistance
• …….
The Oryza longistaminata. A: The panicle of the O.
longistaminata; B: The performance of O.
longistaminata in field; C, D: the strong Rhizome
of O. longistaminata.
A
B D
C
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Additional useful features from O. longistaminata
• A saturated molecular linkage map was constructed (Causse, 1994, Wilson, 1999).
• Xa21, resistance to Bacterial Blight has been cloned with map-based (Khush, 1990; Song, 1995).
• Resistance to tungro viruses has been verified (Angeles E.R. 1998).
• Resistance to root knot nematode M. graminicalawas reported (Imelda R. Soriaano, 1999).
• A saturated molecular linkage map based on PCR markers (Hu, 2003)
• ……
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Goal
• Exploit the possibility of
using perenniality from
O. longistaminata for
development of
cultivars of perennial
upland rice (PUR) or
perennial rice (PR)
Oryza longistaminataPhoto was cited from Vaughan
(1994)
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The Strategy for Perennial Rice Breeding
F1
RD23
O. longistaminata
Progeny derived from F1
by Self-intercross, back cross,
transgenic and MAS with diversity
germplasm of rice
hope to
x
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F1 plant of RD23_Longi
F1
Rhizome
F1 plant in greenhouse
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Problems for the PR Breeding and genetics
1, Less F1 progeny from O. longistaminata
up to now, there are ~6 cases reported for obtaining the F1 progeny since it is difficult to obtain the F1 plant in the procedure of interspecific hybrid between O. sativa and O. longistaminata.
among these cases, although F1 plant can get, most of these absence Rhizome. Including CIRAD’s (Ghesquiere, 1991), IRRI’s(Bara, 2000) and Japanese (Maekawa, 1997), and others not so clear.
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2, Lethal gene
The Rhizome present is Linkage to lethal gene with D1 and D2; (Chu and Oka 1970;
Ghesquiere, 1991)
results to obtain the progeny lack with Rhizome, means the Rhizome is usual lost in the progeny lines during the selection for breeding purpose.
Problems for the PR Breeding and genetics
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3, The reproductive barriers (Hybrid Sterility, Sgene)
This is a popular phenomenon between the cultivar and its wild species, even between two sub-species, Indica and Japonica of O. sativa.
It is also a main factor for obtaining the progeny that combine the favorable traits/gene from receiptor and donors. For example, the yield components.
Problems for the PR Breeding and genetics
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4, Photoperiod sensitivity
From Vegetative growth to productive growth,
the day-light is important for the short day-
light plant, including rice as it derived from
tropical zone.
The progeny with strong ability of vegetative
growth of O. longistaminata is main factor to
lack selection for PR.
Problems for the PR Breeding and genetics
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5, Seed Dormancy
Problems for the PR Breeding and genetics
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6, Shatter grains
Problems for the PR
Breeding and genetics
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7, awn
Problems for the PR
Breeding and genetics
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How to overcome these problems?
All of these problems are the negative effect for PR improvement.
One of is utilization the traditional methods, such as Self-intercross, Backcross, then select the useful progeny.
Other one is understanding the genetic mechanism of these factor, specially for Rhizome, then using the MAS, Transgenic Strategy for cultivars of PR improvement.
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There are two stages for our results:
Phase I is from 1997-2004;
Phase II is from 2005-now.
Progress in PR
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During First stages(1997-2005), there are 6 results has been obtained.
1, Obtaining the F1 plant derived RD23/O. longistaminata. (here, RD23 is an Indica type cultivar of rice)
2, From F2 and the molecular mapping shown that the Two Dominant Complementary Genes (Rhz2 and Rhz3) for Rhizome Expression in O. Longistaminata and mapped on chr3 and chr4, respectively.
3, Rhz2 and Rhz3 have been registered on the International Rice Genetic Committee.
4, The plant of BC2F1 with Rhizome has been obtained.
5, The Rhizome related traits has been QTLs analysis.
6, The first PCR-based molecular genetic map has been constructed.
Progress in Phase I
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The F1 plant of the RD23/O. longistaminata cross was obtained by direct hybridization followed by embryo rescue and had 32.5% pollen fertility, indehiscent anthers, rhizomes that were intermediate in size, and abundance between the parents.
This is a important material for development Cultivar of PR or PUR.
Progress in Phase I
F
1
Rhizome
F1 plant
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Progress in Phase I
Segregation of rhizome trait in the
F2 population based on field experiment
absence
0.8011X2(9:7)
106121Numbers
presenceRhizome
Result :
Two Dominant Complementary,
Rhz2, Rhz3, Genes for Rhizome
Expression in O. Longistaminata
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PCR-base Molecular Genetic Map
Progress in Phase I
RM4280.0RM32313.1RM28319.0RM2530(6)21.6OSR226.6RM27232.3RM29245.7
RM15866.5
RM30687.8RM23797.5RM297109.5RM302114.7RM212118.1RM319118.3RM265122.4RM315128.5
OSR23145.9
RM529154.9
RM4850.0
OSR179.2OSR1415.1RM27925.5RM42331.8RM55536.8
RM17456.8OSR960.0RM32262.9RM7170.5
RM30083.4
RM34196.9
RM327112.7
RM263138.1
RM2421148.6
RM240157.5
RM166177.7
RM213190.1
RM208202.7RM207206.4OSR26214.6
RM600.0
RM23115.2RM5883(10)19.9
OSR1344.0Rhz252.1OSR1653.4RM3657.8RM25166.5RM28275.1RM455184.0RM33889.5
RM156116.2
RM4626133.6RM6097142.7OSR31145.8RM55157.3RM1(1)165.0RM2525167.9
RM4612180.9
RM114203.1
RM442224.2
RM5510.0
RM51810.6
RM26120.6
RM18532.4RM14243.9RM11948.4Rhz350.6RM27357.8RM25265.7
RM31778.3
RM349104.3OSR15106.1RM348107.2RM127116.4RM280123.5
RM1590.0RM1226.4OSR3511.1
RM1327.3RM40534.9
RM24952.4RM50958.0
RM16479.9
RM29193.9RM163101.7RM161107.3
RM421117.0
RM274138.9
RM87151.0
RM334163.6RM31167.6
RM1330.0RM4356.2RM17013.7
RM58726.8
RM51037.6
RM20455.6RM642067.3RM31474.9RM25377.6RM40283.0RM27686.6RM13698.3RM5818105.4
RM6309128.5RM528132.7
RM4509143.4
RM176157.4RM345162.7
OSR21174.5
www.gramene.org
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Mapping for Rhz2
and Rhz3
The molecular
mapping of Rhizome
gene Rhz2 and Rhz3 was
mapped to the interval
between markers OSR16
(1.3 cM) and OSR13 (8.1
cM) on rice chromosome
4 and Rhz2 located
between RM119 (2.2 cM)
and RM273 (7.4 cM) on
chromosome 3.
Progress in Phase I
RM5510.0
RM51810.6
RM26120.6
RM18532.4
RM14243.9RM11948.4
Rhz350.6
RM27357.8
RM25265.7
RM31778.3
RM349104.3OSR15106.1RM348107.2
RM127116.4
RM280123.5
Chromosome 4
RM600.0
RM23115.2RM5883(10)19.9
OSR1344.0
Rhz252.1OSR1653.4RM3657.8RM25166.5RM28275.1RM455184.0RM33889.5
RM156116.2
RM4626133.6RM6097142.7OSR31145.8RM55157.3RM1(1)165.0RM2525167.9
RM4612180.9
RM114203.1
RM442224.2
Chromosome 3
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Progress in Phase II
The segregation and recombination of genotype of two dominant complementary genes, Rhz2 and Rhz3 (9:7 model of Mendelian)
AB Ab aB ab
AB AABB AABb AaBB AaBb
Ab AABb AAbb AaBb Aabb
aB AaBB AaBb aaBB AaBb
ab AaBb Aabb aaBb aabb
AB Ab aB ab
AB AABB AABb AaBB AaBb
Ab AABb AAbb AaBb Aabb
aB AaBB AaBb aaBB AaBb
ab AaBb Aabb aaBb aabb
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The QTLs of Rhizome traits mapping on Chromomsome
Progress in Phase I
RM428
RM323RM283RM2530(6)OSR2RM272
RM292
RM158
RM306RM237RM297RM302RM212RM319RM265RM315
OSR23
RM529
RL
RIL
RM60
RM231RM5883(10)
OSR13Rhz2OSR16RM36RM251RM282RM4551RM338
RM156
RM4626RM6097OSR31
RM55RM1(1)RM2525
RM4612
RM114
RB
D
RIL
TN
RL
RN
RB
N
RIN
RM551
RM518
RM261
RM185
RM142RM119Rhz3RM273RM252
RM317
RM349OSR15RM348RM127RM280
RL
RN
RB
D
RB
N
RIL
RIN
TN
RD
W
RL RN RBD
RBN RIL RIN
TN RDW
Chr1 Chr3 Chr4
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Progress in Phase I
The QTLs of Rhizome traits mapping on Chromomsome
RM159RM122OSR35
RM13
RM405
RM249RM509
RM164
RM291RM163RM161
RM421
RM274
RM87
RM334RM31
RL
RN
RIL
RIN
RB
D
Chr5
RM133RM435
RM170
RM587
RM510
RM204RM6420RM314RM253RM402RM276
RM136RM5818
RM6309RM528
RM4509
RM176RM345
OSR21
RL
RB
D
RIL
TN
RM427
RM481
RM125RM180RM214RM320RM11OSR22RM2826RM336RM234RM18RM47RM134RM118
RL
RB
D
RIL
RM216
RM467
RM271
RM269
RM228
RM333
RM496RM590
RL
RN
RIL
RL RN RBD
RBN RIL RIN
TN RDW
Chr6 Chr7 Chr10
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Progress in Phase I
RM485
OSR17
OSR14
RM279
RM423
RM555
RM174
OSR9RM322
RM71
RM300
RM341
RM327
RM263
RM3421
RM240
RM166
RM213
RM208RM207
OSR26
2Sorghum
LG F
pSB367
pSB107
pSB201
pSB193
pSB341
(M4)
pSB637b
(M4)
pSB038
pSB512 (M4)
pSB094
RG157
Csu173
CDO686
RG437
RM216
RM467
RM271
RM269
RM228
RM333
RM496
RM590
10
SHO68
Csu111a
(M1)
CDO98 QRn10
RM428
RM323
RM283RM3530
OSR2
RM272
RM292
RM158
RM306
RM237
RM297RM302RM212RM319RM265
RM315
OSR23
RM529
1
RZ776
(M3)
pSB614
(M3)
pSB613
(M3)
Sorghum
LG A
QRl1
RM60
RM231
RM6883
OSR13
OSR16RM36
RM251
RM282
RM5551
RM338
RM156
RM4626
RM7097OSR31
RM55
RM1RM3525
RM5612
RM114
RM442
3
Sorghum
LG C
pSB088
(M5)
pSB300A
(M5)
pSB050
(M5)
RZ284
R944
Rhz2
QRn3
QRbd2
QRbn2
Comparative mapping (Rice and Sorghum)
PNAS, 2003, 100:4050-4054
![Page 33: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/33.jpg)
Progress in Phase I
Rhz3
RM427
RM481
RM125
RM180
RM214
RM320
RM11
OSR22
RM3826
RM336
RM234RM18
RM47
RM134
RM118
7
Sorghum
LG B
RZ395
pSB077
R1245
RM159
RM122
OSR35
RM13
RM405
RM249
RM509
RM164
RM291
RM163
RM161
RM421
RM274
RM87
RM334
RM31
5
Sorghum
LG G
R1436
pSB445
pSB069
RM133
RM435
RM170
RM587
RM510
RM204
RM7420
RM314
RM253
RM402
RM276
RM136
RM5818
RM7309
RM528
RM5509
RM176
RM345
OSR21
6
Sorghum
LG I
pSB355
(M6)
CDO17
RZ612
RM551
RM518
RM261
RM185
RM142
RM119
RM273
RM252
RM317
RM349
OSR15RM348
RM127
RM280
4
Sorghum
LG D
pSB428a
(M2)
pSB188
(M2)
RZ69
RZ740a
QRl7
QRi6
QRn5
QRn7
QRin6
Comparative mapping (Rice and Sorghum)
![Page 34: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/34.jpg)
Progress in Phase IRhz2 and Rhz3 gene registration
![Page 35: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/35.jpg)
The BC2F1 Individual
Progress in Phase I
![Page 36: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/36.jpg)
During in the phase II, the action for PR breeding
and genetics are in progress.
The fine mapping of Rhizome genes, Rhz2 and
Rhz3, are on the way;
A lots of the breeding lines for PR purpose have
been evaluated in field;
The pollen grain fertility loci QTL analysis of F2
progeny was detected.
Progress in Phase II
![Page 37: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/37.jpg)
F2 Individual in Field for Rhizome Evaluation and Mapping
Progress in Phase II
![Page 38: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/38.jpg)
F2 plant in field for Rhizome genes fine mapping and breeding lines selection
Progress in Phase II
![Page 39: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/39.jpg)
Experiment: determination of Rhizome expression with 3 replications with random plot design for by cutting method
Progress in Phase II
![Page 40: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/40.jpg)
Result of fine mapping renewed
Progress in Phase II
RM5510.0
RM51810.6
RM26120.6
RM18532.4
RM14243.9RM11948.4Rhz350.6RM27357.8
RM25265.7
RM31778.3
RM349104.3OSR15106.1RM348107.2RM127116.4
RM280123.5
Chromosome 4
RM600.0
RM23115.2RM5883(10)19.9
OSR1344.0Rhz252.1OSR1653.4RM3657.8RM25166.5RM28275.1RM455184.0RM33889.5
RM156116.2
RM4626133.6RM6097142.7OSR31145.8RM55157.3RM1(1)165.0RM2525167.9RM4612180.9
RM114203.1
RM442224.2
Chromosome 3
RM14603
Rhz2
OSR161.1
2.1
RM119
Rhz3
RM1700
0
0.3
0.4
9.528kb~35kb
![Page 41: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/41.jpg)
Progress in Phase II Microarray
In this study, the specific gene
expression patterns across five tissues
in O. longistaminata, especially in the
rhizome were characterized by using the
Affymetrix microarray platform.
Results showed that the different gene
sets were determined exclusively
expressed in five tissues, 58 and 61
genes were identified as prevalent sets
in rhizome tip and internode
respectively. Cis-element analysis and
co-localization of rhizome related QTLs
for the rhizome prevalent gene set were
further performed
1a 1b 1c 2a 2b 2c 3a 3b 3c 4a
4b 4c 5a 5b 5c
The results will be
accepted by Plant
biology of BMC
![Page 42: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/42.jpg)
Progress in Phase II Microarray
0
10
20
30
40
50
60
70
80
Up-regulated Genes
Down-regulated Genes
Functional classification of the differentially expressed
genes in the rhizome tip in comparison with shoot tip.
![Page 43: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/43.jpg)
Progress in Phase II Fosmid library
• The library consists of 110,000 clones, which has insertion with an average size of about 43kb and represents 10 genome equivalents and is no bias (10X). The results indicate that the fosmid library has high quality and deep coverage that is sufficient for target gene isolation, physical mapping,gene functional analysis and so on. The Fosmid library of O. longistaminatais first report.
![Page 44: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/44.jpg)
Breeding lines
Progress in Phase II
BC1 RLR504
• Two rhizome
gene locus are
heterozygote in
O. Longi (AaBb);
• 5% grain filling;
• Normal pollen
fertility
• Strong Rhizome
• Less cultivar-
like plant type
• Short awn
![Page 45: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/45.jpg)
Breeding lines
Progress in Phase II
BC1 RLR540
• Two rhizome
gene locus are
heterozygote in
O. Longi (AaBb);
• 5% grain filling;
• Normal pollen
fertility
• Strong Rhizome
• Less cultivar-
like plant type
• Short awn
![Page 46: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/46.jpg)
Breeding lines
Progress in Phase II
200808_11 BC1 plant
• Two rhizome gene
locus are homozygote
in O. Longi (AABB);
• 75% grain filling;
• Normal pollen
fertility
• Strong Rhizome
• Self-compatibility
• Less cultivar-like
plant type
• Short awn
![Page 47: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/47.jpg)
Breeding lines (F2) AaBb AaBB AABb AABB
Progress in Phase II
36-1 F2 plant AaBb
Two rhizome
gene locus are
heterozygote;
Normal grain
filling;
Strong rhizome
presence
![Page 48: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/48.jpg)
Breeding lines
Progress in Phase II
• One rhizome gene
locus is heterozygote,
other one is
homozygote
• Normal grain filling;
• Normal pollen fertility
• Strong rhizome
presence
• Self-compatibility
22-93 F2 plant AaBB
![Page 49: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/49.jpg)
Progress in Phase II
34-31 AABb • One rhizome gene
locus is heterozygote,
other one is
homozygote
• Normal grain filling;
• Normal pollen fertility
• Strong rhizome
presence
• Self-compatibility
Breeding lines
![Page 50: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/50.jpg)
Progress in Phase II
14-2 AABB
• Two rhizome gene
locus are homozygote
•
• Normal grain filling;
• Normal pollen fertility
• Strong rhizome
presence
• Self-compatibility
Breeding lines
![Page 51: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/51.jpg)
Breeding lines
Progress in Phase II
• Two rhizome gene
locus are homozygote
in O. longi;
• 75% grain filling;
• Normal pollen fertility
• Rhizome absence
• Self-compatibility
• Awn less
6-28 F2 pant
![Page 52: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/52.jpg)
Breeding lines
Progress in Phase II
• Two rhizome gene
locus are homozygote;
• 75% grain filling;
• Normal pollen fertility
• Rhizome absence
• Self-compatibility
10-25 F2 plant
![Page 53: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/53.jpg)
Breeding lines
Progress in Phase II
• Rhz2 is heterozygote,
Rhz3 is homozygote;
• 70% grain filling;
• Normal pollen fertility
• Rhizome absence
• Self-compatibility
• Cultivar-like plant type
• Short awn12-38 F2 plant
![Page 54: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/54.jpg)
Spikelet fertility improvement
Progress in Phase II
Rang:
0%-90%
above
0% ~2% ~10% ~30% >50% >90%
![Page 55: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/55.jpg)
• NILs with Rhizome construction
Progress in Phase II
0% ~2% ~10% ~30% >50% >90%
F2
F3
F4
F5
Fn
NILs with Rhziome
![Page 56: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/56.jpg)
Reproductive Ability after three times for cutting of F2 population
Progress in Phase II
Aug.,
2007
Feb.,
2008
Aug.,
2008
Sep.,
2007
Apr.,
2008
After Aug.,
What happen?
Dec. 2009??
2010???
Oct.,
2007
![Page 57: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/57.jpg)
Progress in Phase II
F3 population from 34-31(F2) used to reproduced ability
test from 2008 to now
![Page 58: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/58.jpg)
Progress in Phase II
Reproductive Ability after three times for cutting
2008 2009 2010
![Page 59: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/59.jpg)
• Genetic study on perenniality of rice
• Perennial rice breeding
• Other perennial crops screening in Kunming
Progress in Phase II(2010)
![Page 60: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/60.jpg)
Two rhizome gene cloning
1, Whole genome de novo sequence of O. longistaminata (finished and data analysis on going)
2, Fine mapping
3, Candidate gene of Rhz2 and Rhz3
4, Transformation for candidate genes
Progress in Phase II(2010)
![Page 61: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/61.jpg)
Whole genome sequence of O. longistaminata
70X coverage the physical
map of O. longistaminata
![Page 62: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/62.jpg)
Transgenic results
RI CE_13428 3 Os03t 0216000- 01Si mi l ar t o Zi nc- f i nger pr ot ei n
KNUCKLES, Zi nc f i nger , C2H2- t ype
RNAi
and
Overpress
AABB as
receiptor
![Page 63: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/63.jpg)
Perennial Rice Breeding
1, NILs
2, Obtained the materials with Rhizome in F3,
F6, F7 with different genotype
3, Problem?
Progress in Phase II (2010)
![Page 64: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/64.jpg)
NILs of RD23/O. longistaminata
• F7 population with 336 family lines
• Rhizome with normal pollen fertility
• Larger varieties of agronomic traits: plant high, tiller,
• Favorable genes mining, stem borer-resistance
![Page 65: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/65.jpg)
Breeding materials for PR
F3 lines from 22-93(AaBB)
Two plants, AaBB, aaBB
22-93(18), aaBB
22-93(36), AaBB
MAS
![Page 66: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/66.jpg)
F3 lines from 34-31 (AABb)
Two plants, AABb, AAbb
Breeding materials for PR
34-31(6-12), AABb
34-31(4-29), AAbb
MAS
![Page 67: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/67.jpg)
• F6 and F7 lines from NILs (AABB)
Breeding materials for PR
F6
F7F6
![Page 68: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/68.jpg)
• Problems and prospect
Progress in Phase II (2010)
![Page 69: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/69.jpg)
Rhizome gene Rhz2, Rhz3 cloning
Hope to understand the genetics of Rhizome
and using transgenic method for perennial
rice breeding.
Next step
![Page 70: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/70.jpg)
Next step
Large population (F3, F4, F5 ) in MAS with 4
different genotypes: AaBB, AABb, AaBb and
AABB.
Hope to select the plant with rhizome and
good fertility panicle.
![Page 71: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/71.jpg)
Next step
The individuals with Rhizome and
Fertility after selected screening both
Upland and Aerobic land.
![Page 72: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/72.jpg)
Next step
International Cooperation
2009, in field at experimental station in Sanya, China
![Page 73: Progress in Perennial Rice Breeding and Genetics...O.eichingeri Peter 24 CC Sri Lanka,Africa O.rhizomatis Vaughan 24 CC Sri Lanka O.punctata Kotschy ex Steud. 24,48 BB,BBCC Africa](https://reader034.vdocuments.mx/reader034/viewer/2022043002/5f7f6cddaa8d31574b4e00d0/html5/thumbnails/73.jpg)
Next step
Washington State University: perennial wheat
The Land Institute: perennial sorghum, sunflower, wheat, +.
University of Manitoba: (potentially) perennial rye, wheat
Yunnan Academy of Agricultural Sciences: perennial rice
FFI-CRC: perennial wheat
云南农科院,多年生水陆稻
澳大利亚美国土地研究所
Washington State University: perennial wheat
The Land Institute: perennial sorghum, sunflower, wheat, +.
University of Manitoba: (potentially) perennial rye, wheat
Yunnan Academy of Agricultural Sciences: perennial rice
FFI-CRC: perennial wheat
International Network of Perennial Crops
云南农科院,多年生水陆稻
澳大利亚美国土地研究所
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NATURE|Vol 456|4 December 2008
可能改变世界的5个作物科学家
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Acknowledgment
Financial support from
• National Natural Science Foundation
of China
• The Land Institute
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Dr. E. Sacks
Prof. Dayun Tao
Thanks to….
The UR Group
Peng Xu
Xiangneng Deng
Jiawu Zhou
Jin Li
Wei Deng
Qiong Li
Lijuan Li
Yang Yu
Wenting Wan
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Thank you for your attention!