prof. mohamed labib salem's students
DESCRIPTION
A summary of the master and PhD works under the supervision of Prof. Mohamed Labib SalemTRANSCRIPT
Director, Dr. Mohamed L. Salem, PhD
Prof. of Immunology
Tanta University, Egypt
Center of Excellence in Cancer Research
(CECR)
Location
Tanta University Educational Hospital
Location
Established Date
June 2013 through a project funded by Science and
Technology development Fund (STDF)
PI: Prof. Mohamed Labib Salem
Affiliation: Tanta University
Established Date
Dr. Mohamed L.SalemProf. of immunology
Dr. Said HammadProf. of clinical Pathology
Dr. Mohamed RamadanProf. of Pediatric
Dr. Mohamed AttiaAssis. Prof. of Clinical Pathology
Dr. Abdel-Aziz ZidanPhD of Immunology
Ms. Mona Fouad ZidanResearcher Assistant
Research Team
Research Team
Organogram
Correlating immunologic and clinical performancebetween genomic, transcriptomic and proteomicprofiling of circulating tumor cells versus primarytumor cells
CECR Main Project
CECR Core Facility
Nomination:The BD FACSCanto derives its name from the musical term “bel canto” meaning
operatic singing that focuses on ease and purity of tone with an agile and precise
vocal technique.
Flow Cytometry Unit
• BD FACSCanto II Cytometer
• The BD FACSCanto system has a capacity for three lasers, eight-parameter
detection while capturing particle sizes from 0.5 to 50 μm in diameter.
• Digital electronics for processing up to 10,000 events per second, and a novel
sample injection tube supporting
Multiparameter detection helps in:
• Advanced cell subset analysis.
• Multiparameter functional studies.
• Soluble protein detection.
• Quantification studies for cell-surface antigens and soluble proteins.
• Decreases the total sample volume necessary for a specific project as more
parameters.
Specifications of the instrumentSpecifications of the instrument
• Immunophenotyping by flow cytometry is an important tool in the diagnosis and
staging of patients with a haematological neoplasm as well as solid tumors.
• Some of the flow cytometry applications in diagnosis of different diseases are listed
below:Detection of
minimal residual disease
BD FACSCanto Applications
Stem cell enumeration
Analysis of Leukemias and
Lymphomas
Reticulocyte analysis
Detection of Immunodeficiency
diseases
Detection of HIV infection
Analysis of different types of solid
tumors.
Detection of autoantibodies
Research & Clinical applications of the instrumentSpecifications of the instrument
The IsoFlux system enriches rare cells from biological samples and prepare them for further analysis , It gives immediate access to high-quality , viable cells in a high density format , ready for use in the application of your choice
Benefits:
• Access a wide range of samples.
• Use your own capture criteria.
• Capture intact, pure cells.
• Minimize Sample Diluation
• Save Time
• Save Space
• Utilize validated CTC analysis workflows.
Cell Sorting Unit
Cell Sorting Unit
IsoFlux Applications
Enumeration ImmunofluorescenceFluorescence in situ
hybridization
Next generation sequencing
Mutation detection Gene expression
Microarray
A high throughput technology that allows detection of thousands of genes simultaneously.
Features:
• High samples throughput.
• Laser excitation at 532nm, dual- laser excitation .
• Scanning pixel resolution 2µm
• Scan sequence: simultaneous scan and independent scan.
• Scan/read time: approximately 5 samples/hour.
Genomic & Proteomic Unit
Genomic & Proteomic Unit
Microarry Applications
TransiptomemRNA ExpressionmiRNA ExpressionSplice Variance
Genome StructureDNA Copy number
Genome VariationSingle Nucleotide Polymorphism
Copy number Variations
ProteomeProtein-Protein InteractionsDNA – Protein inreraction
EpigeneticsDNA Methylation
Chromatin Structure
• LumineX200
Genomic & Proteomic Unit
Flexible Multiplexing Technology
• Open Platform for Genomic & Proteomic assays
• Life Science Research & Diagnostic assays available.
• Broad menu of commercial assays and laboratory developed tests
• Multiplex up to 500 tests in one well of microliter plate.
• Decrease sample volume requirements.
• Reduce assay reagent volume, expense & labor.
• Generate more information on interrelationships between analytes within a single sample.
Genomic & Proteomic Unit
Genomic & Proteomic Unit
Luminex Applications
Clinical Diagnostics Clinical Research Life Science Research
Biosurveillance Assay Development Tools
CECR CollaboratorsCECR Collaborators
Monday 24 February 2014 The 1st Annual Conference of
The Center of Excellence in Cancer ResearchNext Generation Cancer Research & Diagnosis
In Collaboration withResearch Centers of Excellence
(STDF- Affiliated Centers)
CECR Conferences
CECR PhD. & MSc. Students
Thank You !