product overview allergen analysis - r-biopharm ag – allergen analysis 2011 6 allergens...

Download Product Overview Allergen Analysis - R-Biopharm AG – Allergen Analysis 2011 6 Allergens Product Description

Post on 23-Mar-2020

1 views

Category:

Documents

0 download

Embed Size (px)

TRANSCRIPT

  • R-Biopharm AG

    Product Overview Allergen Analysis

    2011

  • 2R-Biopharm AG – Allergen Analysis 2011

    Allergens

    Allergen Lateral-Flow ELISA real-time PCR Enzymatic

    Almond   

    Brazil Nut 

    Casein 

    Cashew Kernel  

    Celery 

    Coconut 

    Crustacean  

    Egg  

    Fish 

    Gliadin/Gluten   

    Glutamic Acid 

    Hazelnut   

    Histamine 

    ß-Lactoglobulin 

    Lactose 

    Lupine  

    Lysozyme 

    Macadamia nut 

    Milk  

    Mollusc 

    Mustard   

    Peanut   

    Pistachio  

    Sesame  

    Soya  

    Sulfite 

    Walnut  

    R-Biopharm‘s Test Kit Portfolio

  • 3

    Allergens

    Advantages of ELISA:

    • quantitative results using certified standards (e.g. NIST CRM) • unique method to dectect milk and egg protein • simple sample preparation (20 min) and test procedure (3 x 10 min) • possibility of using automates (e.g. Chemwell) • evaluation with the software RIDA®SOFT Win

    RIDASCREEN®FAST Tests Quantify allergenic protein

    bioavid Lateral Flow Tests Swab for on-site testing

    SureFood® Allergen real-time PCR

    Gluten Reaction Mix 1 Store at -20°C

    Lot No: 04449

    SureFood® Allergen real-time PCR

    Gluten Inhibition Control Mix 2 Store at -20°C

    Lot No: 04449

    SureFood® Allergen real-time PCR

    Gluten Positive Control DNA 3 Store at -20°C

    Lot No: 04449

    SureFood® Allergen real-time PCR

    Gluten Taq Polymerase 4 Store at -20°C

    Lot No: 04449

    SureFood® Allergen real-time PCR

    Gluten

    FDE 5 Store at -20°C

    Lot No: 04449

    SureFood® Tests Uniform sample preparation

    Surface and hygiene control

    Clean and controlled allergen production conditions are a prerequisite for allergen-free food products. Therefore, swabs within production sites should be carried out regularly with test strips from bioavid or RIDA®QUICK. No lab equipment is required and results from these immunochromatographic tests are available within 5 - 10 min.

    Product testing

    For food testing different analytical methods exist: ELISA and PCR. While an ELISA detects proteins; PCR is detecting DNA. These two methods are ideal for reciprocal confirmation of screening results. RIDASCREEN® ELISAs detect directly allergenic proteins using a microtiter-plate-photometer. SureFood® Allergen from Congen is based on real-time PCR. For sample preparation SureFood® PREP (S1012) is used to extract DNA.

    Advantages of real-time PCR:

    • robust, stable target molecule (DNA) in highly processed food samples • highly specific system with minimum tendency to cross reactions • sensitivity in “low ppm” range • one sample preparation (90 min) for 15 parameters • standardized handling and test procedure (1 - 2 hrs) • in development: real-time PCR allowing quantification

    Your turn-key solution to ensure allergen free food

    Allergen Analysis on Surfaces and in Food with highly specific and sensitive test kits using ready-to-use reagents

    Even small traces of allergenic substances in food can provoke allergic reactions in sensitized persons. Therefore, monitoring of cross contaminations in raw materials and production lines as well as correct labelling of food products are an important part of quality control in the food industry.

  • 4R-Biopharm AG – Allergen Analysis 2011

    Allergens

    Product Description No. of Tests/Amount Code No. Enzyme immunoassay for quantitative analysis

    RIDASCREEN® Gliadin AOAC-RI certified Codex Alimentarius Method (Type 1)

    Official R5 Mendez method: Sandwich ELISA to quan- tify prolamines from wheat, rye and barley in e.g. food declared as gluten-free. Sample extraction with R7006 or R7099 (not contained in the kit). Detection limit: 1.5 mg/kg (ppm) gliadin or 3 mg/kg (ppm) gluten

    96 determinations Incubation time: 1 hr 30 min

    R7001

    RIDASCREEN®FAST Gliadin R5 sandwich ELISA to quantify prolamines from wheat, rye and barley in e.g. food declared as gluten-free. Sample extraction with R7006 or R7099 (not contained in the kit). Detection limit: 2 mg/kg (ppm) gliadin or 4 mg/kg (ppm) gluten

    48 determinations Incubation time: 30 min

    R7002

    RIDASCREEN® Gliadin competitive (2nd generation)

    R5 competitive ELISA to quantify potential toxic peptide sequences from prolamines from wheat, rye and barley in fermented and hydrolyzed food (e.g. beer, starch, starch syrup, malt extracts). The standard material is a hydrolysate (mixture of wheat, rye and barley); the results can be related to the limit values of the Codex Alimentarius. Detection limit: 1.36 mg/kg (ppm) Gliadin

    96 determinations Incubation time: 40 min

    R7021

    Cocktail Solution Extraction solution for all e.g. heat treated food samples in conjunction with R7001 and R7002. The extraction with Cocktail Solution is the official recommended method (Mendez).

    105 ml R7006

    RIDA® Extraction Solution Alternative to the Cocktail Solution: The extraction is faster (35 min compared to 1 hr 50 min with the Cocktail). It is used for all e.g. heat treated samples in conjunction with R7001 and R7002. R7099 is also used for Casein extraction of bakery goods and sausages (see R4612).

    105 ml R7099

    Set of 3 Gliadin Assay Controls

    Three control materials: 1 negative, 2 positive (homogenized flour samples)

    3 x 1.5 g R7010

    RIDASCREEN®FAST Milk Sandwich ELISA to quantify milk proteins (casein and ß-lactoglobulin) in food containing traces of milk components. The assay is calibrated to NIST SRM 1549 non fat milk powder. Detection limit: 0.7 mg/kg milk protein

    48 determinations Incubation time: 30 min

    R4652

    RIDASCREEN®FAST Casein (2nd generation)

    Sandwich ELISA to quantify casein in food containing traces of milk or casein/caseinates. Detection limit: 0.24 mg/kg (ppm) casein in ice cream, chocolate, beverages. Detection limit: 1.36 mg/kg (ppm) casein in bakery goods and sausages (extraction with R7099, not contai- ned in the kit)

    48 determinations Incubation time: 30 min

    R4612

    RIDASCREEN®FAST ß-Lactoglobulin

    Sandwich ELISA to quantify native and processed ß-lactoglobulin in food containing traces of milk or whey. Detection limit: 0.19 mg/kg (ppm) ß-lactoglobulin

    48 determinations Incubation time: 30 min

    R4902

    RIDASCREEN® ß-Lactoglobulin

    Competitive ELISA to quantify native and processed ß-lactoglobulin in hydrolyzed milk products (e.g. hypoallergenic baby food). Detection limit: 0.115 mg/kg (ppm) ß-lactoglobulin

    96 determinations Incubation time: 2 hr 45 min

    R4901

  • 5

    Allergens

    Product Description No. of Tests/Amount Code No. Enzyme immunoassay for quantitative analysis

    RIDASCREEN® FAST Ei/Egg Protein

    Sandwich ELISA to quantify traces of egg in food. The assay is calibrated to NIST SRM 8445 whole egg powder. No cross-reactivity to chicken meat (raw and cooked). Detection limit: 0.27 mg/kg (ppm) whole egg powder

    48 determinations Incubation time: 30 min

    R6402

    RIDASCREEN®FAST Lysozym

    Sandwich ELISA to quantify traces of lysozyme in wine, cheese and sausage. Detection limit: 0.02 mg/kg (ppm) lysozyme

    48 determinations Incubation time: 30 min

    R6452

    RIDASCREEN®FAST Peanut AOAC-RI certified

    Sandwich ELISA to quantify traces of peanut in food. The assay is calibrated to NIST SRM 2387 peanut butter. Detection limit: 1.5 mg/kg (ppm) peanut

    48 determinations Incubation time: 30 min

    R6202

    RIDASCREEN®FAST Hazelnut

    Sandwich ELISA to quantify traces of hazelnut in food. Detection limit: 1.5 mg/kg (ppm) hazelnut

    48 determinations Incubation time: 30 min

    R6802

    RIDASCREEN®FAST Mandel/Almond

    Sandwich ELISA to quantify traces of almond in food. Detection limit: 1.7 mg/kg (ppm) almond

    48 determinations Incubation time: 30 min

    R6901

    RIDASCREEN®FAST Senf/ Mustard

    Sandwich ELISA to quantify traces of mustard in food. The assay detects yellow, white, brown and black mustard. Detection limit: 0.22 mg/kg (ppm) mustard powder

    48 determinations Incubation time: 30 min

    R6152

    RIDASCREEN®FAST Lupine Sandwich ELISA to quantify traces of lupin in food. Detection limit: 0.6 mg/kg (ppm) lupin protein

    48 determinations Incubation time: 30 min

    R6102

    RIDASCREEN® Histamin Competitive ELISA to quantify histamine in food. Detection limit: 0.1 - 100 mg/kg (ppm) histamine depending on matrix

    96 determinations 48 determinations Incubation time: 1 hr 15 min

    R1601 R1604

    RIDA®QUICK Histamin

    Colourimetric assay to quantify histamine in fishmeal and fresh fish. Detection limit: 20 mg/kg (ppm) (fresh fish) Detection limit: 125 mg/kg (ppm) (fish meal)

    48 determinations Incubation time: 5 min

    R1603

    RIDA® Sampling Tools for Swab Tests

    Plastic tubes and swabs 100 of each Z0010

    Test Strips (immunochromatographic tests)

    RIDA®QUICK Gliadin The immunochromatographic test is based on the R5 antibody and detects prolamines from wheat, rye and barley. The strips can be used directly for swa

Recommended

View more >