Revised 09/2007

Product Information Sheet and Operating Instructions Vivapore 2, 5, 10/20 – For Clinical Chemistry For biological samples of 2, 5 and 10/20 mL

Vivapore Concentrators (top left Vivapore 2, bottom left Vivapore 5, right Vivapore 10/20) Concentrate urine, cerebrospinal fluid, or plasma to intensify proteins indicating abnormal or pathological states (e.g., Bence Jones proteins in urine), for analysis by electrophoresis or immunoelectrophoresis).

Features

• Individual, disposable units • Efficient concentration (up to 750X depending on product selected) • Printed, easy-to-read graduation marks indicate concentration level • High speed concentrations, even with particle-laden solutions • 10,000 Dalton membrane cutoff • Up to 98% recovery rate • No additional equipment required • Dead Stop Volume of 20 µl or 50 µl • Never over-concentrates

Applications

• Concentrating proteins in urine (Bence-Jones protein) • Concentrating other clinical samples prior to electrophoresis or immunoelectrophoresis • Simple concentration of biological samples • Improved band visualization of dilute protein solutions following electrophoresis • Increasing sensitivity for subsequent testing of cerebrospinal fluid or other dilute body fluids (e.g., human milk, saliva,

semen, tears, cervical and amniotic fluids, sputum) • Enrichment of serum or plasma • ELISA test enhancement for initial protein concentrations below detection limits • Cell washing and harvesting • Buffer exchange or desalting • Concentrating enzymes or isoenzymes (alkaline phosphatase, lactic dehydrogenase, etc.)

Vivapore concentrators offer exceptional ease of use and flexibility for the concentration and/or purification of macromolecules from dilute solutions. Depending on the configuration and reservoir volume of the Vivapore selected, starting volumes may vary from 0.5 to 20mL and up to 400X concentration may be achieved. Membranes used in Vivapore devices are highly hydrophyllic and have been specially formulated to provide low protein binding and high filtration speed without the need for pressure or vacuum.

Revised 09/2007

Operation 1. Concentrating macromolecules

a. Insert Vivapore 5 or 10/20 into stand. Vivapore 2 is self-standing. b. Pipette sample through aperture at the top of the device. Device can be left unattended until desired

concentration is achieved. Solvent and micromolecules are pulled through the membrane by a high capacity absorbent. A dead stop feature prevents the sample from concentrating to dryness.

c. Once the desired volume is achieved, the concentrate is withdrawn using a Pasteur, thin plastic or gel loader type pipette. The same is now ready for further analysis.

2. 20mL capacity expansion for the VP10/20

Sample capacity of the VP10/20 can be increased to 20mL by the use of the ancillary reservoir (purchased separately). First pipette 10mL into the Vivapore10/20, then lock the ancillary reservoir to the top opening and add up to an additional 10mL of sample.

Bence-Jones Protein Recovery The level at which Bence-Jones proteins indicate a pathological condition is 0.06mg/mL (Damacco). The Vivapore is able to recover and allow visualization of at least 0.008mg/mL Bence-Jones proteins – well below that required by the clinician. (Ref. Damacco, Waldestrom, Acta Medica Scandinavica (1968) 184 403-409.) SPECIFICATIONS:

Product Name Vivapore 2 Vivapore 5 Vivapore 10/20 Brand Name Vivascience Vivascience Vivascience Device Color Blue Clear Clear

Device Volume 0.5 – 2.5 mL 1 – 5 mL 2 – 10/20 mL Filter Type PES

Deadstop Volume/Recovery Volume

20 µl 50 µl 50 µl

MWCO (molecular weight cutoff)

7,500 7,500 7,500

Number Tests/Pack 30 30 or 100 30 or 100 Max. Concentration Factor 200X 100X 400X

Part # Description

VP0201 Vivapore 2 static concentrator, 7500 MWCO, Pack of 30 (self-standing) VP0501 Vivapore 5 static concentrator, 7500 MWCO, Pack of 30*

VP0502 Vivapore 5 static concentrator, 7500 MWCO, Pack of 100*

VP2001 Vivapore 10/20 static concentrator, 7500 MWCO, Pack of 30* VP2002 Vivapore 10/20 static concentrator, 7500 MWCO, Pack of 100*

VPA002 Pack of six (6) disposable stands for use with Vivapore 5 and Vivapore 10/20

VPA005 Pack of 100 Plastic Recovery Pipettes for Vivapore 10/20 VPA006 Pack of 10 expansion reservoirs for use with Vivapore 10/20 VPA007 Pack of 100 Plastic Recovery Pipettes for Vivapore 5

*One disposable stand included with 30 and 100 packs

For more information or to order, contact your distributor:

Protein Separation and Purification Technologies

521 Great Road Littleton, MA 01460

Tel: (800) 456-4633 (Toll Free) Fax: (877) 289-8482 (Toll Free)

www.vivascience-us.com