processing of clinical specimen for immunofluorescence staining & viral culture exercise ii –...
TRANSCRIPT
Processing of Clinical Specimen for Immunofluorescence staining & Viral
culture
Exercise II – Day 4 Virology Section
Department of MicrobiologyJIPMER
XIII NATIONAL WORKSHOP ON “DIAGNOSTIC METHODS IN CLINICAL MICROBIOLOGY”
8th – 11th October, 2014
XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER
2
Introduction
Viral detection methods:•Staining
Direct stainingImmunofluorescence staining
• Culture Cell culture Animal / embryonated egg inoculation
• Enzyme immunoassays• PCR methods
XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER
3
Objective
To observe and understand the steps in processing a clinical
specimen for immunofluorescence staining and viral culture
XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER
4
Steps – Processing of specimen
Sample
receipt
Vortex &aliqu
ot
Centrifug
e
Use for Viral culture
Use for Immunofluorescence staining
XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER
5
IFA
XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER
6
Positive IFA- Adenovirus Negative IFA
XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER
7
Detection of virus growth in cell cultures
Cytopathic effect
Metabolic inhibition
Hemadsorption
Interference
Transformation
Immunofluorescence
Normal cell line – Hep2 CPE- Adenovirus
XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER
Grape like clusters of round cells
Polygonal cells
Thank you
XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER