Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand *e-mail: g5237169@student.mahidol.ac.th

Abstract: In Thailand, there are reports of gibbon species on illegal trade. Gibbons are smal apes that are threatened to extinction. They are listed in appendix I of CITES. In this study molecular markers were developed to use as identification tool for 3 gibbons Hylobares lor Hylobates pileatus, and Nomascus concolor. Species-specific PCR primers were designec based on polymo~phisms of the mitochondria1 cytochmme b gene. Experiment! demonstrated that optimal magnesium concentration for this primer set was 2.0mM. Increast of annealing temperature in the thennocycling condition clearly increased the specificity 01

all 3 amplification reactions. Eaeh primer pair can specifically amplify its target specie! when amplification was carried out under high smngent condition. No PCR product wen detected when specificity of these primers were tested against human, dog and pig DNA.

H-HOOSS: SURFACE PLASMON RESONANCE STUDIES O F ESTROGEN BINDING TO THE ESTROGEN RECEPTOR A: NEW INSIGHT INTO RECEPTOR-LWAND INTERACTIONS Purada Chokoraiakchad', Insee Sensom', Toemsak Srikhirin2.', Darawan pinthong'.' I Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

Center of Intelligent Materials and Systems, Faculty of Science, Mahidol University. Bangkok 10400, Thailand 'e-mail: sctsk@mahidol.ac.th; scdpt@mahidol.ac.th

Abstract: Estrogenic drugs or compounds have recently been widely used by the general population. A simple assay method for characterizing the specificity and efficacy is thus very important especially in drug screening. Estmgen receptor alpha ( E k ) is a superfamily of estrogen receptor, which can interact with ligand and activate cell proliferation and growth of breast cancer. Surface plasmon resonance (SPR) is a powerful technique for detecting an analyte without any labeling. In this study, we have developed the equilibrium condition for observing the specificity of & binding to ERJERE complex on biotin-polyethylene glycol (biotin-PEG) coated surface sensor. To get optimum condition, we used 100 pglml of streptavidin (SA), 0.5 pM of DNA complex and 0.1% ween for preventing non-specific binding. The dissociation rate constant (k,,) that we investigated was 2 nM. The result was closed to an equilibrium dissociation constant (Kd) 1.8 -+ 0.6 nM of ER interaction with ERE sequences in vitro under equilibrium conditions by fluorescence anisotropy.

H-H0059: Staphylococcus aureus INFECTION DECREASES NUMBER O F CILIA ON OSTEOBLASTIC CELLS Anuohan ~ittichokechaiwut'.', Niratcha p ha is om boon^, Suttimas yuakyon& I Department of Preventive Dentistry, Faculty of Dentishy, Naresuan University, Phitsanulok 65000, Thailand

Research laboratory, Faculty of Dentistry. Naresuan University, Phitsanulok 65000, Thailand *email: anuphans@nu.ac.th

Abstract: The non-motile primary cilium is a hair-like solitary cellular smcture consisting of a membrane continuous with the cell membrane. This smchlre is presented in almost all vertebrate cells and have recently become the focus of research interest because loss of primary cilia function or defect of primary cilia controlling is associated with human diseases and developmental abnormalities. This non-motile primary cilum serves as a mechanosensor and chemosensor in bone cells. The aim of this study was to investigate the number of primary cilia between normal and infected MC3T3-El osteoblastic cells. Cells

238 37' Conpss on Science and Technology of Thailand

Preventive

Dentistry

Preventive

Dentistry