Dot-blot enzyme immunoassay for the detection of bovine viral diarrhea virus antibodies

Dot-blot enzyme immunoassay for the detection of bovine viraldiarrhea virus antibodiesPresented By :I Made Adi SuardhyanaI Kadek Fuck Ther SutejaNi Luh Gede Dicha Vivi SaputriIntroductionBovine viral diarrhea virus (BVDV), a member of the genus Pestivirus, causes a range of symptoms in cattle, leading to widespread economic losses worldwidePrenatal infection can also result in immunotolerance and persistent infection (PI) in calvesAnimals persistently infected (PI) with BVDV are regarded as the major source of spread of infection within and among herdsIntroductionVarious detection methods have been developed, most extensively used protocols for serodiagnosis of BVD are based on the plate ELISA and serum neutralization (SN) methodsEach of these methods may have disadvantages and objective of this study was to develop and evaluate a convenient and inexpensive sytem for detection of BVDVMaterials and methodsSampling1250 sera were collected from twenty-eight herds with a history of BVDV infection included 809 individual SN negative sera.SN and ELISA testsStep virus neutralization testSeraheat-inactivatedwere initiallyAntibody titersmeasured againstUse microtitrationmethodNADL strain of BVD virusMaterials and methods Serial twofold dilutions of sera in duplicate

50 L of Dulbeccos modifified EaglesWas preparedthan mixed50 L of virus suspension containing of BVD

Mixture50 L of virus suspension containing of BVD

Incubation for 1h at 37 C