Respirometry chambers

Fernando Estellés, Salva Calvet & David Yañez-Ruiz

2 rabbitchambers

2 rabbitchambers

CH4 chambers at EEZ-CSIC

video

CH4 chambers at EEZ-CSIC

CH4 chambers at EEZ-CSIC

GI tract

1. Calibration of gas analyzer

2. Recovery test

Calibration gas analyzer

Calibration gas analyzer

Recovery test

Recovery test

Recovery test

1 hour releasing CH4

CH4 flow (mL/min) Air flow out (m/s) Air flow out (L/min) Expected concentration (ppm)

Measured concentration (ppm) % Recovery

Chamber 1 54,54 3,5 1648,5 33,08 31 93,70Chamber 2 55,04 3 1413 38,95 37,4 96,01Chamber 3 55,04 3,5 1648,5 33,39 32 95,84Chamber 4 55,55 3,35 1577,8 35,21 35,25 100,12

Recovery test

CO2 injection

(95 grams)

Recovered: 80,34 grams(84,6%)

System operation

0

5

10

15

20

25

30

35

40

45

50

9:36

10:4

611

:56

13:0

614

:16

15:2

616

:36

17:4

618

:56

20:0

621

:16

22:2

623

:46

0:56

2:06

3:16

4:36

5:46

6:56

8:06

CH4 CON

CH4 CLOU

CH4 T3

CH4 T4

-10

-5

0

5

10

15

20

9:36

10:4

611

:56

13:0

614

:16

15:2

616

:36

17:4

618

:56

20:0

621

:16

22:2

623

:46

0:56

2:06

3:16

4:36

5:46

6:56

8:06

H2 CON

H2 CLOU

H2 T3

H2 T4

24 hourly patternCH4 H2

ppm

0

5

10

15

20

25

30

35

40

45

50

9:04

10:1

411

:24

12:3

413

:44

14:5

416

:04

17:1

418

:24

19:3

420

:44

21:5

423

:04

0:14

1:24

2:34

3:44

4:54

6:04

7:14

CH4 CON

CH4 CLOU

CH4 T3

CH4 T4

-10

-5

0

5

10

15

20

9:04

10:1

411

:24

12:3

413

:44

14:5

416

:04

17:1

418

:24

19:3

420

:44

21:5

423

:04

0:14

1:24

2:34

3:44

4:54

6:04

7:14

H2 CON

H2 CLOU

H2 T3

H2 T4

CH4 H2

ppm

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