preparation of competent e. coli cells preparation of probes by pcr digestion of pbr322 and plasmid...
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Preparation of competent E. coli cells
Preparation of probes by PCR
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Ligation of fragments with pBR322 plasmid
Transformation of the ligated construct into pBR322
Selection of recombinant E. coli cells
Isolation and purification of the pBR322 plasmid with insert
Digestion of pBR322 with restriction enzymes
.Identification of inserts by electrophoreses and Southern blotting
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Ligation of fragments with pBR322 plasmid
Preparation of competent E. coli cells
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Ligation of fragments with pBR322 plasmid
Transformation of the ligated construct into pBR322
Preparation of competent E. coli cells
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Ligation of fragments with pBR322 plasmid
Transformation of the ligated construct into pBR322
Selection of recombinant E. coli cells
Preparation of competent E. coli cells
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Ligation of fragments with pBR322 plasmid
Transformation of the ligated construct into pBR322
Selection of recombinant E. coli cells
Isolation and purification of the pBR322 plasmid with insert
Preparation of competent E. coli cells
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Ligation of fragments with pBR322 plasmid
Transformation of the ligated construct into pBR322
Selection of recombinant E. coli cells
Isolation and purification of the pBR322 plasmid with insert
Digestion of pBR322 with restriction enzymes
Preparation of competent E. coli cells
Preparation of probes by PCR
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Ligation of fragments with pBR322 plasmid
Transformation of the ligated construct into pBR322
Selection of recombinant E. coli cells
Isolation and purification of the pBR322 plasmid with insert
Digestion of pBR322 with restriction enzymes
.Identification of inserts by electrophoreses and Southern blotting
Preparation of competent E. coli cells
Preparation of probes by PCR
Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV
Purification of the digested fragment
Ligation of fragments with pBR322 plasmid
Transformation of the ligated construct into pBR322
Selection of recombinant E. coli cells
Isolation and purification of the pBR322 plasmid with insert
Digestion of pBR322 with restriction enzymes
.Identification of inserts by electrophoreses and Southern blotting