praktikum 1 biokimia

7/18/2019 praktikum 1 biokimia

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LAPORAN PRAKTIKUM

BIOKIMIA

NAMA

NIM

KEL.PRAKTIKUM/KELAS

JUDUL

:

:

:

:

MUHAMMAD RIZKY

08121006024

B/GENAP

DETEKSI GULA PEREDUKSI

LABORATORIUM BIOKIMIA

JURUSAN FARMASI

FAKULTAS MATEMATIKA DAN ILMU

PENGETAHUAN ALAM

UNIERSITAS SRI!IJAYA

201"

LAPORAN PRAKTIKUM



BIOKIMIA 1

NOMOR PRAKTIKUM : I

NAMA PRAKTIKAN : MUHAMMAD RIZKY

NIM : 08121006024

JUDUL PRAKTIKUM : DETEKSI GULA PEREDUKSI

TUJUAN :

Mahass!a "a"#$ "%"aha" #&'s# (%)%*s +$,a #%&%($*s s%-a&a $"$"

.a'+ "%&$#a*a' *%)%&a"#,a' (asa& (a,a" /(a'+ *%ah,a' /*"a *,'*

PRINSIP KERJA :

M%,a*$*a' #&'s# (%)%*s +$,a #%&%($*s (%'+a' &%a*s %h,'+ (a' /%'%(-)

DASAR TEORI

Ka&/h(&a) "%&$#a*a' s%'.a!a .a'+ )%&/%')$* (a& ",%*$, *a&/'

h(&+%' (a' *s+%'3 Ka&/h(&a) a(a,ah #,sa*a&(a "%&$#a*a' s$"/%& %'%&+

$)a"a #a(a "a*a'a'3 Nas *%)%,a a+$'+ a(a,ah /%/%&a#a -')h "a*a'a'

"%'+a'($'+ *a&/h(&a)3

Ka&/h(&a) a(a,ah s%'.a!a .a'+ "%'+a'($'+ $'s$&5$'s$&: H (a' O

)%&$)a"a )%&(a#a) ((a,a" )$"/$h5)$"/$ha' .a)$ *&a5*&a 793 D'a"a*a'

*a&/h(&a) *a&%'a s%'.a!a5s%'.a!a ' s%/a+a h(&a) (a& *a&/' (a,a" s%'.a!a

)%&s%/$) #%&/a'('+a' a')a&a H (a' O s%&'+ 2 /%&/a'('+ 1 s%#%&) a&3

Ja(6H12O6 (a#a) ()$,s 6;H2O<6 12H22O11 s%/a+a 12;H2O<11 (a'

s%)%&$s'.a (a' #%&$"$sa' %"#&s ()$,s s%/a+a 'H2'O' a)a$ ' ;H2O<'3

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sa)'.a )%&ha(a# =a)5=a) #%'+h(&,ss3 Ka&/h(&a) a)a$ +$,a (/a+ "%'a( %"#a)

*%,as #**:

13 G$,a .a'+ s%(%&ha'a a)a$ "'sa*a&(a *%/a'.a*a' a(a,ah s%'.a!a5

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a,(%h(a .a'+ (s%/$) a,(sa (a' #,h(&*s *%)' (s%/$) *%)sa3

23 O,+sa*a&(a s%'.a!a /%&s ($a a)a$ ,%/h +$,a s%(%&ha'a .a'+

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+$,a ' (s%/$) *a)a' +,*s(a3

>3

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(%*s)&'33

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+a,a*)sa3 U')$* (sa*a&(a -')h'.a a(a,ah ,a*)sa (a' "a,)sa3 S%(a'+*a' .a'+

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a(a'.a s)&$*)$& &a')a )%&/$*a s%h'++a )(a* &%')a' )%&ha(a# #&s%s *s(as

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+$,a &%($*s ( (a,a" )$&$'a' a",$"@#a) (s%/$) (%'+a' (%?)&s% %$a,%')

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/%')$* D5 .a*' D5+,$*sa3

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sa"#a #,"%&3 Ka&/h(&a) .a'+ (*'s$"s )$/$h $"$"'.a ($/ah "%'a(

+,$*sa (a' "%'+a,a" s&*$,as (a,a" )$/$h ;(a,a" (a&ah "%'+a'($'+ 0089

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h(&,sa a",$" G,$*sa /asa'.a )%&s%(a (a,a" /%')$* =a) /%&!a&'a #$)h a)a$*&s)a, #a(a)3 G,$*sa (#&($*s s%-a&a *"%&s, "%,a,$ h(&,ss #a) (%'+a'

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s%/a+a +,$s),3 D"a'a +$+$s a,(%h(a #a(a +,$*sa (+a') "%'a( +$+$s

a,*h, (%'+a' a(a'.a #%'a"/aha' +as h(&+%'

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()a"/ah*a' (%'+a' ,a&$)a' #%&a* ')&a) a"'a* a*a' )%&a( -%&"' #%&a*3

M%&%($*s ,a&$)a'5,a&$)a' %h,'+ .a)$ (%'+a' )"/$,'.a %'(a#a' *$#& *s(a

La&$)a' %h,'+ )%&(& a)as ,a&$)a' %h,'+ A: ,a&$)a' $SO4 (a' ,a&$)a' %h,'+

B: ,a&$)a' (a& Na5K5)a&)&a)

ALAT DAN BAHAN



LALU MASING MASING LARUTAN DIPANASKAN SELAMA 20 DETIKLALU DITAMBAHKAN DENGAN NaOH 2 TETES SEBAGAI INDIKATORSEHINGGA BERUBAH FARNA3 HITUNG DENGAN STOPFATH LAMA

PERUBAHAN YANG TERJADI SEMENJAK DITAMBAHKAN NaOH

BUAT AMPURAN GLUKOSA LARUTAN BENEDIT DENGAN AIRDENGAN KONSENRASI PERBANDINGAN BENEDIT DAN AIR DENGAN

KONSENTRASI 1:1 1:2 1:> 1:4

SIAPKAN ALAT DAN BAHAN

A,a) :

Ta/$'+ &%a*s

Ra* )a/$'+ &%a*s

G%,as $*$&

P%'a'+as a&

S#!a)-h

Baha' : La&$)a' B%'%(-)

La&$)a' %h,'+ A (a' B

La&$)a' +,$*sa

La&$)a' A$a(%s))

ARA KERJA UJI BENEDIT



MASING MASING LARUTAN DIPANASKAN HITUNG LAMATERJADINYA PERUBAHAN FARNA DENGAN STOPFATH

BUAT GLUKOSA DENGAN KONSENTRASI MENAMPRKAN HLING ADAN B MASING 10 TETES DENGAN MEMASUKKAN EHLING A

TERLEBIH DAHULU BARU EHLING B SETELAH ITU TAMBAHKANDENGAN AIR DENGAN KONSENTRAS EHLING DAN AIR 1:1 1:2 1:> 1:4

SIAPKAN ALAT DAN BAHAN

ARA KERJA UJI ELING



DATA HASIL PENGAMATAN

13 D%'+a' &%a+%' B%'%(-)

KONSENTRAS

I

DITAMBAH

BENEDIT

HASIL DENGAN

PEMANASAN

SELAMA 20

DETIK

SETETES NaOH

FAKTU

N&"a, @ &a'#a

a&

B%&!a&'a a+a* /&$ B%&!a&'a -*,a) 7 (%)*

1 : 1 +,$*sa

(a' a&

B%&!a&'a a+a* /&$ *,a) -%'(%&$'+

'++a

17 (%)*

1:2 +,$*sa (a'

a&

B%&!a&'a a+a* /&$ B%&!a&'a -*,a)

"$(a

24 (%)*

1: 4 +,$*sa (a'

a&

B%&!a&'a a+a* /&$ B%&!a&'a '++a

-%'(%&$'+ &a'+%

4 (%)*

23 D%'+a' &%a+%' %h,'+ ; %h,'+ A B <

K's%')&as DITAMBAH

EHLING A

DITAMBAH

EHLING BDIDIAMKAN

SELAMA 1

MENIT

HASIL DENGAN

PEMANASANSELAMA 20

DETIK

SETETES NaOH

FAKTU

N&"a, @

&a'#a a

B%&!a&'a /&$

)%&a'+

5A!a, : B&$

5S%)%,ah ((a"*a' :

Ha$

B%&!a&'a "%&ah

/a)a

1> D%)*

1 : 1 +,$*sa

(a' a&

B%&!a&'a /&$

)%&a'+

5A!a, : B&$

5S%)%,ah ((a"*a' :Ha$

B%&!a&'a "%&ah

/a)a

17 D%)*

1:2 +,$*sa

(a' a&

B%&!a&'a /&$

a+a* +%,a#

5A!a, : B&$

5S%)%,ah ((a"*a' :

Ha$

B%&!a&'a "%&ah

/a)a

2 D%)*

1: 4 +,$*sa B%&!a&'a /&$ 5A!a, : B&$ B%&!a&'a "%&ah 4 D%)*



(a' a& a+a* +%,a# 5S%)%,ah ((a"*a' :

Ha$

/a)a

LAMPIRAN GAMBAR DATA HASIL PENGAMATAN

P%'a"/aha' +,$*sa

B%'%(-) (a' #%'a"/aha''.a

A$a(%s)



La&$)a' %h,'+ #%'a"/aha' (a' has,'.a



PEMBAHASAN

P%&%a*s B%'%(-) /%&$#a ,a&$)a' .a'+ "%'+a'($'+ *$#& s$,a) 'a)&$"

*a&/'a) (a' 'a)&$" s)&a)3 U (,a*$*a' (%'+a' "%'a"/ah*a' &%a+%') B%'%(-)

*%(a,a" ,a&$)a' *a&/h(&a) (a' (,a*$*a' #%"a'asa'3 S%)%,ah (,a*$*a' #%"a'asa'

)%&a( #%&$/aha' #a(a /%/%&a#a ,a&$)a' .a)$ G,$*sa &$*)sa (a' ,a*)sa3

P%&$/aha' ' /%&$#a )%&/%')$*'.a %'(a#a' "%&ah /a)a #a(a ,a&$)a' )%&s%/$)3 P&s%s #%'('+'a' *%)+a ,a&$)a' ' )(a* "%"#%'+a&$h #%&$/aha' #a(a %'(a#a'3

S%(a'+*a' ,a&$)a' .a'+ ,a' /%&!a&'a /&$ .a'+ "%&$#a*a' !a&'a as, (a& &%a+%'3

Ga,a*)sa s%'(& "%'+a,a" s%(*) #%&$/aha' s%)%,ah (#a'as*a' .a)$ )%&(a#a)

!a&'a "%&ah #a(a /a+a' #%&"$*aa' ,a&$)a' (a' s%)%,ah (('+'*a' !a&'a '

"%'+h,a'+3 Ga,a*)sa "%&$#a*a' sa,ah sa)$ *a&/h(&a) .a'+ "%'+a'($'+ +$+$s

a,(%h( (a,a" #%'+$a' )%&(a#a) +a,a*)sa )(a* )%&a( #%&$/aha' .a'+ (ha&a#*a'

s%#%&) +,$*sa &$*)sa (a' ,a*)sa3 Ha, ' "$'+*' )a/$'+ )%,ah )%&*')a"'as

,%h s%'.a!a ,a' s%h'++a )(a* "%'+has,*a' %'(a#a' s%#%&) ha,'.a *a&/h(&a)

,a' .a'+ "%'+a'($'+ +$+$s *%)' (a' a,(%h(3

B%&(asa&*a' has, #%'+a"a)a' (a)as /ah!a (a& /%&/a+a "a-a" %'s ,a&$)a'

*a&/h(&a) s%)%,ah (&%a*s*a' (%'+a' ,a&$)a' B%'%(-) )%&'.a)a &%a*s'.a )(a*

)%&a( s%-a&a /%&sa"aa'3 Ha, ' (s%/a/*a' )%&+a')$'+ a)as *'s%')&as *a&/h(&a)

.a'+ (#%&*sa /a* (sa*a&(a a)a$ "'sa*a&(a3 Pa(a (asa&'.a (a& /%&/a+a

"a-a" %'s ,a&$)a' *a&/h(&a) .a'+ ($ )%&'.a)a .a'+ /%&%a*s #s) (%'+a'

B%'%(-) ha'.a +,'+a' (sa*a&(a (a' "'sa*a&(a s%(a'+*a' ,a&$)a'

#,sa*a&(a s%#%&) a",$" )(a* (a#a) /%&%a*s (%'+a' B%'%(-)3 E'(a#a'5%'(a#a'

.a'+ (has,*a' #a(a #%&-/aa' ' a(a,ah "%&ah3 K$''+ )%&+a')$'+ a)as *$a)'.a

,a&$)a' +$,a3 B%'%(-) $+a /%&sa) /asa ,%"ah *a&%'a a(a'.a 'a)&$" *a&/'a) (a'

'a)&$" s)&a)3



Pa(a $ %h,'+ "%'++$'a*a' ,a&$)a' %h,'+ .a'+ )%&/$a) (a& ,a&$)a'

)%"/a+a s$,a) (%'+a' 'a)&$"5*a,$" )a&)a) (a' 'a)&$" h(&*s(a3 U %h,'+s

(+$'a*a' $')$* "%'+%)ah$ a(a'.a +$,a #%&%($*s (a,a" sa"#%,3 Da& 4 sa"#%,

()%)%s s%/a'.a* 10 )%)%s ,a&$)a' %h,'+ A (a' B ;1:1< .a'+ )%,ah (-a"#$&*a'

s%/%,$"'.a3 La,$ (#a'as*a' (a,a" !a)%&/a)h s%,a"a 20 (%)* (a' (a"a) sa"#a

"$,a /%&$/ah !a&'a (a' )%&/%')$* %'(a#a'3 P%"a'asa' (,a*$*a' $')$*

"%"#%&-%#a) &%a*s3 U %h,'+s (+$'a*a' $')$* "%'+$ +,$*sa3 Da& *%%"#a)

sa"#%, )%&/%')$* %'(a#a' /%&!a&'a "%&ah )$a@ "%&ah /a)a33 La&$)a' %h,'+s (a#a)

"%'+*s(as +,$*sa .a'+ "%'+a'($'+ +$+$s a,(sa .a'+ "%",* +$+$s

a,(%h(a3 E'(a#a' .a'+ )%&/%')$* "%&$#a*a' U2O *a&%'a ,a&$)a' %h,'+s A (a'

B (a#a) "%'+has,*a' ' U2 s%/a+a #%'+*s(as sa"#%, .a'+ "%'+a'($'+a,(%h(3

P%'+%'-%&a' +,$*sa .a'+ )%&a( s%)%,ah sa"#%, (a' &%a+%' (#a'as*a'

s%,a"a 20 (%)* (a' ()a"/ah*a' (%'+a' NaOH has,'.a &%,a) (a' )(a* /%&/%(a

a$h (%'+a' *%,"#* *%,"#* ,a''.a3 a&as !a*)$ ' )%&a( *a&%'a S%"a*'

/a'.a* +,$*sa (a' "a*' (*) a& &%a*s a*a' s%"a*' -%#a)3

P%'a"/aha' NaOH ()$$*a' s$#a.a +,$*sa /%&%a*s s#%s* (%'+a' /asa

*a&%'a ,a&$)a' /%'%(-) a)a$ %h,'+ /%,$" -$*$# "a"#$ $')$* "%"/%&*a' &%a*s

.a'+ s#%s* a')a&a +$,a (%'+a' s /asa3S%,a' )$ ' $2 ha'.a (a#a) (%'(a#*a'

s%/a+a $2O ha'.a *a (a,a" s$asa'a /asa s%h'++a (a#a) "%"/%&*a' !a&'a .a'+

*has3



KESIMPULAN

13 K)a "%'++$'a*a' &%a+%' /%'%(-) (a' &%a+%' %h,'+ s%/a+a '(*a)&

$')$* "%'(%)%*s +$,a

23 P%&/a'('+a' G,$*sa (a' a& .a'+ (+$'a*a' a(a,ah '&"a, a)a$ )a'#a a&

1:1 1:2 1:4

>3 P%"a'asa' (+$'a*a' $')$* "%"#%&-%#a) &%a*s a')a&a &%a+%' (a' s%'.a!a

43 P%'a"/aha' NaOH ()$$*a' s$#a.a +,$*sa /%&%a*s s#%s* (%'+a' /asa

*a&%'a ,a&$)a' /%'%(-) a)a$ %h,'+ /%,$" -$*$# "a"#$ $')$* "%"/%&*a'&%a*s .a'+ s#%s* a')a&a +$,a (%'+a' s /asa

3 a&as !a*)$ &%a*s #%&$/aha' !a&'a )%&a( *a&%'a S%"a*' /a'.a* +,$*sa

(a' "a*' (*) a& &%a*s a*a' s%"a*' -%#a)3



DATAR PUSTAKA

13 %ss%'(%' Ra,#h J3a'( Ja' S3 173 O&+a'- h%"s)&. >&(

23 %(3 B%,"a') a,&'a3 I&a!a' A'!a&3 20073 Ka&/h(&a)3 P&)' S#&)

S-%'-% a'( P%&&"a'-% La/3 III 1: 154

>3 Ha'('3 Ka&/h(&a) Pa(a U K$a,)a)3 Ba'($'+3 2003 Da*s%s )a'++a, 6

Ma&%) 201> #$*$, 1:00

4. Martoharsono, Soeharsono. 1975. Biokimia. Gadjah Mada University Press. :

Yoyakarta

5. Poedjiadi, !nna. 1994. "asar#"asar Biokimia. U$#Pres : %akarta

praktikum 1 biokimia

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