PRACTICAL SCIENCE 101 Safety

MicroscopyFrugal Science - Foldscope

★ LISTEN to your instructors.★ Always FOLLOW the directions provided for the lab.★ Do not EAT or DRINK anything in the lab. ★ Please be OFF your CELLPHONES during lectures or

experiments. It is disrespectful and disturbing to others. You may be allowed to use your phones only for taking pictures or videos.

General Safety

★ Tie back LOOSE HAIR.

★ Roll up LOOSE SLEEVES.

★ Please keep your HANDS AWAY from your face. It may cause serious irritations or pain if chemicals get in your eye.

General Safety

★ Never SMELL, TASTE, or TOUCH any chemicals in the lab.

★ If an accident or a spill occurs, please NOTIFY THE TEACHERS.

★ After working with chemicals, always WASH YOUR HANDS

with soap & water.

★ PROPERLY DISPOSE chemicals as instructed to do so.

General Safety

Personal Protective Equipment (PPE)★ Wear SAFETY GOGGLES at all times during an experiment.

★ GLOVES should be worn to protect your hands from touching

chemicals when appropriate.

★ Always wear closed-toe shoes in the lab. No sandals or

slippers in the lab!

★ Aprons/lab coats will be given if necessary.

★ Know the LOCATION of the fire extinguisher, fire blanket, eyewash station, and safety shower.

★ Use FIRE BLANKET to extinguish fires on PEOPLE★ Although it is unlikely, if a fire occurs in the laboratory, you

should FOLLOW THE DIRECTIONS of your instructors. Remember the acronyms RACE and PASS. Do not panic.

Fire Precautions

FIX THE LAB #1

FIX THE LAB #2

Intro to Microscopy● A microscope is an instrument used to see objects that

are too small to be seen by the naked eye.● Microscopy is the science of investigating small objects

and structures using such a microscope.● The first simple compound microscope was made by

Robert Hooke in the 1600s.

Parts of a Microscope

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1. Eyepiece/Ocular lens2. Diopter adjustment3. Nosepiece4. Arm5. Objective lens6. Slide holder7. Stage8. Condenser9. Iris Diaphragm

10. Coarse focus knob11. Fine focus knob12. Light source13. Base14. On/Off Switch

Foldscope and Frugal ScienceFrugal science: The development of ultra low-cost tools for the improvement and accessibility of science in underdeveloped and developing regions

● Ex. 3D Fuge, Electropen, 3D printed pipettes

Foldscope: an “ultra-affordable, paper microscope” that is considered to be the cheapest microscope in the world. It was developed by Manu Prakash at Stanford University. It is capable of magnifying 140x.

Credits: Prakash Lab at Stanford

Onion Cells Algae Mosquito

Examples

Credit: Microcosmos Community

Procedure and MaterialsMaterials● Plastic pipette or dropper● Sterile, individually packed cotton swabs● Glass microscope slides● Plastic coverslips● Paper towels or tissue

Protocol:1. Pipette one drop of pond water onto microscope slide.2. Place clear plastic cover over sample on microscope slide.3. Remove any excess solution by allowing a paper towel to touch one side of the

coverslip.4. Place the slide in the Foldscope, with 4 x or 10 x objective in position and find a cell.

Then view your slide at higher magnification.

JEOPARDY - LET’S REVIEW

https://docs.google.com/presentation/d/1ViM_jcfKhzQitAV0xuX4wvi4dTfyP0lcPMivuhe_E3E/edit?usp=sharing

Always listen to your instructor

WHAT SAFETY PROCEDURES SHOULD ONE

TAKE IN THE LAB?

WHAT IS THE FOLDSCOPE + HOW

DOES IT WORK? WHAT IS MICROSCOPY?

A microscope is an instrument used to see objects that are too small to be seen

by the naked eye.

Foldscope: an “ultra-affordable, paper

microscope” that is considered to be the

cheapest microscope in the world. It was developed by Manu Prakash at Stanford University. It is capable of

magnifying 140x.

TODAY WE LEARNED...

THANK YOU!Does anyone have any final questions?

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THE END.