practical -git spotters (amirul aiman's conflicted copy 2013-07-05)
DESCRIPTION
TTRANSCRIPT
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Important Parasites
Entamoeba histolytica - AmoebiasisGiardia lamblia - GiardiasisCryptosporidium parvum CryptosporidiasisTaenia solium/T saginata (tape worms)Ascaris lumbricoides (round worms)Trichuris trichiura (Whip worm)Ancylostoma duodenale (Hook worm)Enterobius vermicularis (pin/thread worm)
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Irregular in shape with PseudopodiaSize varies from 10-60 microns
Active and motileSpherical nucleus with central karyosome
In acute case, trophozoites are with engulfed erythrocytes
Trophozoites divide by binary fission
Trophozoite
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Trichome stain - Trophozoites
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Spherical , 10-20 micronsFour nucleiMass of glycogen1-4 chromidial bars
CYST
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Trichome stain - cystIodine mount - Cyst
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Adhesive DiskOr Suction Disc
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Trophozoite Cysts Giardia intestinalisTrophozoite pear shaped with 2 nuclei , 8 flagella & a suction disk (for attachment to intestinal wall)Cyst Oval, 4 nuclei, and internal fibers
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A = cyst B= trophozoite of Giardia lamblia
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Cryptosporidium parvum oocysts Acid Fast staining
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Tape worm Taenia saginata(5-10 meters)Adult wormEggEgg : Bile stained, 3 pairs of hooklets
Cannot differentiate eggs of Taenia solium (pork tapeworm) from Taenia saginata (Beef tapeworm)
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The eggs measure 30-35 micrometers in diameter and are radially-striated. The internal oncosphere contains six refractile hooks.
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Trichuris trichura adult wormsResembles whip, 2-5 cms
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Whipworm Trichuris trichiura Adult wormEggEggEgg: it is barrel shaped, 50 x 20m in size. It is brownish (bile stained) and has a translucent polar plug at either ends.
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Adult worm of Enterobius vermiculais
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Adult worm of Enterobius vermiculais
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Egg: 50 to 60 u by 25 , persimmon seed-like, colorless and transparent, thick and asymmetric shell, with larva.
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Ancylostoma duodenale-Hook wormNon bile stained- ColourlessEgg with blastomeres
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Ascaris lumbricoides : Round worm
Cylindrical , 15-40 cms long
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Fertilized egg ((60 x 45m) : Bile stained (brownish yellow)Oval, mammiliated thick albuminous coat Unfertilized egg (80-95u): Elongated, NonfertileAscaris lumbricoides : Round worm eggs
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Vibrio cholerae Gram negative, Comma shaped bacilli
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Campylobacter jejuniComma, S, girdle, spiral shaped