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Practical Biochemistry1 LabBIOCA2101

IUG, Spring 2014Dr. Tarek M Zaida

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• Instructor InfoDr. Tarek M. Zaida Faculty of Science/ Administration Building/ Room B304Phone: Office:2697 Mobile: 059-7193442Email: tzaida@iugaza.edu or tarekzaida@gmail.com

• Office hours:

Sat & Mon: 11-13Sun, Tue, Wed: 10-11

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Course Description

• Introduction of students majoring in Biochemistry to some of the most widely used experimental procedures in biochemistry.

• During this course students will become familiar with the principles of basic biochemistry through the very carefully selected lab activities that will focus on the fundamental biomolecules of the living system such as:

amino acids, proteins, carbohydrates, and lipids

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• This lab will focus mainly on:Qualitative tests of Carbs., Amino acids,

Proteins, and Lipids Using different widely applied methods in

biochemistry labs.

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• Prerequisite:

• Practical Analytical Chemistry CHEM 2110• Biochemistry I BIOCA2301

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Course Objectives

• By the end of this course students will be:• Ready to explain the different roles of the very

important biomolecules in the living system, through understanding the biochemical properties of such compounds.

• More self-confident in conducting experiments with their own hands.

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• Able to handle the different lab equipments with confidence.

• Familiar with the different methods applied in basic biochemistry labs.

• Students will:• Improve their communication skills• Get used to a team-work.

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Textbook • Laboratory Manual in Biochemistry1 (Dr.

Ahmed Thabet, 2005)

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Evaluation

• There will be different types of evaluation that will include:

• Reading the lab manual before the lab activity day• Answering the pre-lab questions during lab session, • Quizzes (2)• Writing Lab reports• Participation in the actual lab work.• Participation in the discussion with classmates

through the electronic learning tools.

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Course Policy

• Attendance all lab sessions is a mandatory.• Zero tolerance for not following given instructions during

lab sessions.• Wearing the lab coat as soon as you step in the lab. • Acting responsibly during the entire lab session.• Team work is a must, including communication with group

partners.• Giving up reports from the previous lab session must be

electronically and before the day of the next lab.• Quizzes will be given only electronically on a previously

announced date and time.• No make ups are allowed.

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Grading Activity Points

Participation in electronic learning

tools

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Quizzes (2) 12

Lab Reports 10

Active Engagement during lab

session

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Final Exam40

Total grades 100

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• Following up with the CourseStudents may reach the course materials through moodle system anytime.In case of anticipating any type of problems, student may contact me at my mobile or via email.

• How to use moodle systemStudents may go through the moodle system using guide found on the website of electronic learning http://elearning.iugaza.edu.ps, under educational resources for students. Students may also ask for assistance from the electronic learning center found in the IT building I400, phone 1573.

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Course TimingActivity

Lab Activity 1 Carbohydrates

Lab Activity 2 Reactions of Reducing Sugars

Lab Activity 3 Phenyl Hydrazine Reaction

Lab Activity 4 Paper Chromatography of Sugars

Lab Activity 5 Lipids

Lab Activity 6 Saponification

Lab Activity 7 Proteins 1

Lab Activity 8 Proteins 2

Lab Activity 9 Precipitation of Proteins

Lab Activity 10 Enzymes

Lab Activity 11 Metabolism

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• Let’s get started..

And have fun working biochemistry