ppe & safety in lab - prac. microbiology
TRANSCRIPT
![Page 1: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/1.jpg)
![Page 2: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/2.jpg)
PERSONAL PROTECTIVE EQUIPMENT (PPE)
![Page 3: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/3.jpg)
Personal Protective Equipment (PPE)
“Specialized clothing or equipment worn by an employee for protection against infectious materials”
![Page 4: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/4.jpg)
How Can I Select PPE?
ICC HEEPF ICEP
PPE selected for use should:Protect HCP clothing and skin from
contamination by blood and body fluids.Protect patients from acquiring
microorganisms from HCP.
![Page 5: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/5.jpg)
What are the types of PPE?
GlovesGowns and apronsMaskEye gogglesOverheadFoot protection ???
![Page 6: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/6.jpg)
Types of Gloves
Sterile GlovesNon-sterile Gloves.Heavy-duty Gloves.
![Page 7: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/7.jpg)
Sterile Gloves
Disposable, individually wrapped.
They are mostly used during surgery and during performing invasive procedures.
![Page 8: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/8.jpg)
Non- Sterile Gloves
Disposable, single use, usually not singly wrapped.
They are mostly used to protect skin against exposure to blood and body fluids
(e.g. when changing dressing and handling specimens).
![Page 9: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/9.jpg)
Heavy – Duty Glovesreusable after decontamination
Used for: Handling medical waste Performing environmental
cleaning
![Page 10: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/10.jpg)
Aprons and Gowns
Used during procedures that generate splashes of blood or body fluids.
Apron Gown
![Page 11: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/11.jpg)
Eye Goggles
Used for protection against splashes of blood or
body fluids (eg. during certain surgical procedures).
![Page 12: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/12.jpg)
Overhead
Used to contain hair during surgical procedures
![Page 13: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/13.jpg)
MasksPlaced over nose, mouth and
chin
Standard mask Used when there is risk of
exposure to droplets containing infectious agents
N95 masksUsed when there is risk of
exposure to airborne infectious agents such as M. tuberculosis (prevent >95% of particles less than 1 micron).
![Page 14: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/14.jpg)
Closed Foot WearIn operating theatre
Over-shoes
“NOT RECOMMENDED”
Ideal way of transferringmicro-organisms from floor and shoes to the hand.
![Page 15: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/15.jpg)
What’s WRONG ?????
![Page 16: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/16.jpg)
Safety in the laboratory
![Page 17: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/17.jpg)
The laboratory safety procedures must be read prior to attending the laboratory session.
![Page 18: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/18.jpg)
WHY??
Important in prevention of: Infection Injury by harmful
chemicals Mechanical injury by
glassware, open flames, and sharp objects.
![Page 19: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/19.jpg)
Personal belongings
away from workstation
Wash your hands with soap at the
- start of the laboratory session
- before performing any procedures
- before leaving the laboratory at the end of the session.
![Page 20: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/20.jpg)
Personal Protective Equipment (PPE) used:
o Gloves are essential.
o Lab coats are required.
o Safety glasses ( goggles) may be required to avoid splashes.
![Page 21: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/21.jpg)
Avoid touching objects (e.g., pencils, cell phones, door handles) while wearing gloves.
![Page 22: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/22.jpg)
Long hair must be tied back or covered to minimize fire hazard or contamination of experiments.
![Page 23: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/23.jpg)
Laboratory personnel should not wear sandals
![Page 24: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/24.jpg)
![Page 25: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/25.jpg)
Smoking in the lab is essentially prohibited
![Page 26: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/26.jpg)
Eating and drinking in the laboratory are absolutely prohibited.
![Page 27: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/27.jpg)
All cultures should be handled as potentially pathogenic.
Liquid cultures must always be kept in a test tube rack.
Broth cultures must never be pipetted by mouth.
![Page 28: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/28.jpg)
In case of Blood Spills
Cover with paper towel.
Apply disinfectant.
Leave for 15 minutes.
Remove while wearing gloves.
Discard in a red bag.
![Page 29: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/29.jpg)
Report all accidents, no matter how minor, to your supervisor!!
![Page 30: PPE & Safety in Lab - Prac. Microbiology](https://reader036.vdocuments.mx/reader036/viewer/2022081419/5561e541d8b42af10c8b4cb7/html5/thumbnails/30.jpg)
BE CAREFUL
BE SAFE