powerpoint プレゼンテーション2015.igem.org/files/presentation/ut-tokyo.pdf · 2015. 10....
TRANSCRIPT
![Page 1: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/1.jpg)
![Page 2: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/2.jpg)
![Page 3: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/3.jpg)
![Page 4: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/4.jpg)
Regenerative medicine and tissue formation is really important in recent years.
Regenerative Medicine Tissue Formation
![Page 5: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/5.jpg)
We need to understand mechanisms of development to regenerate our body.
Regenerative Medicine Tissue Formation
![Page 6: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/6.jpg)
Turing Patterns exist everywhere in the natural world.
![Page 7: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/7.jpg)
Simple mechanism makes complex Turing Pattern.
![Page 8: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/8.jpg)
Because of the complexity of an organisms’ development, it is difficult to investigate Turing Pattern in vivo.
![Page 9: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/9.jpg)
In order to understand mechanisms of development…
OUR GOAL: to create synthetic system forming Turing Pattern.
![Page 10: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/10.jpg)
![Page 11: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/11.jpg)
Two factors have three features to form Turing Pattern.
Features:1.Activation.2.Inactivation.3.Inhibitors diffuse
faster.
![Page 12: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/12.jpg)
Two factors have three features to form Turing Pattern.
Features:1.Activation.2.Inactivation.3.Inhibitors diffuse
faster.
![Page 13: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/13.jpg)
Two factors have three features to form Turing Pattern.
Features:1.Activation.2.Inactivation.3.Inhibitors diffuse
faster.
![Page 14: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/14.jpg)
Two factors have three features to form Turing Pattern.
Features:1.Activation.2.Inactivation.3.Inhibitors diffuse
faster.
![Page 15: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/15.jpg)
The demonstration of the interaction of activator and inhibitor.
![Page 16: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/16.jpg)
![Page 17: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/17.jpg)
Basic Strategy: System
![Page 18: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/18.jpg)
Lateral Inhibition by E. coli and AHL reaction
![Page 19: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/19.jpg)
Local Activation by E. coli self-reproduction
![Page 20: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/20.jpg)
E. coli as activator and AHL as inhibitor
![Page 21: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/21.jpg)
E. coli emits AHL and AHL kills E. coli
![Page 22: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/22.jpg)
Lateral Inhibition: AHL is emitted outside from E. coli.
![Page 23: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/23.jpg)
Lateral Inhibition: AHL activates Barnase.
![Page 24: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/24.jpg)
E.coli emits AHL and AHL kills E. coli
![Page 25: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/25.jpg)
Fig. 1 time development in basic strategy
Convergent when positive feedback is weak.
![Page 26: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/26.jpg)
Meaning of Positive Feedback
![Page 27: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/27.jpg)
Meaning of Positive Feedback
![Page 28: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/28.jpg)
Meaning of Positive Feedback
![Page 29: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/29.jpg)
Fig. 1 time development in basic strategy
Fluctuation will be 0 without positive feedback
![Page 30: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/30.jpg)
![Page 31: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/31.jpg)
Improved Strategy: System
![Page 32: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/32.jpg)
Lateral Inhibition by reactions between E. coli and AHL
![Page 33: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/33.jpg)
Local Activation by the mutual inhibition
![Page 34: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/34.jpg)
Comparing two strategies: System
Basic Strategy Improved Strategy
![Page 35: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/35.jpg)
Comparing two strategies: System
Basic Strategy Improved Strategy
![Page 36: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/36.jpg)
Comparing two strategies: System
Basic Strategy Improved Strategy
![Page 37: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/37.jpg)
The growth rate of improved strategy is smaller in the beginning.
Basic Strategy Improved Strategy
![Page 38: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/38.jpg)
The growth rates are the same at the end.
Basic Strategy Improved Strategy
![Page 39: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/39.jpg)
It is growth rate of growth rate that counts.
Basic Strategy Improved Strategy
![Page 40: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/40.jpg)
Basic Strategy: solid Improved Strategy: pattern
From the same condition, it forms pattern only in improved strategy.
![Page 41: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/41.jpg)
Turing Pattern Simulation of improved strategy.
Fig. 3 pattern by mathematical simulation
![Page 42: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/42.jpg)
Co-activator produces Colicin and Colicin kills activator E. coli.
![Page 43: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/43.jpg)
Local Activation: co-activator repress activator by Emitting Colicin E3.
![Page 44: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/44.jpg)
Colicin E3 has some advantage.
1. Molar weight is large enough:
60000 ~ 61000 Da
2. Safe: only affects microbes.
Soelaiman, S., Jakes, K., Wu, N., Li, C., & Shoham, M.
(2001). Crystal structure of colicin E3: implications for cell
entry and ribosome inactivation.Molecular cell, 8(5), 1053-
1062. Figure 2. A
![Page 45: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/45.jpg)
Local Activation: co-activator repress activator by Emitting Colicin E3.
![Page 46: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/46.jpg)
Local Activation: activator repress co-activator because of growth competition.
![Page 47: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/47.jpg)
Local Activation: Mutual Inhibition
![Page 48: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/48.jpg)
Co-activator produces Colicin and Colicin kills activator E. coli
![Page 49: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/49.jpg)
![Page 50: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/50.jpg)
The way to achieve our goal.
![Page 51: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/51.jpg)
Detailed plan to achieve first step.
a. Diffusion rate of E. colib. Strength of growth inhibitionc. Activity of Colicin Lysis Protein
![Page 52: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/52.jpg)
a. Diffusion rate
![Page 53: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/53.jpg)
Approach for calculating the diffusion rate.
2h
11h
19h
Semi-solid agar gel
![Page 54: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/54.jpg)
The stronger expression of CheZ, the bigger diffusion rate.
Fig. 1 Motility control by CheZ
0
0.8
1.6
Strong2 CheZ Strong1 CheZ Medium CheZ CheZ - (NC)
Dif
fusi
on
Rat
e [
mm
^2/h
]
![Page 55: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/55.jpg)
b. Strength of growth inhibition
![Page 56: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/56.jpg)
Barnase repressed the growth of E. coli as we expected.
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
0 20 40 60 80 100 120 140
OD
60
0
time[hr]
Plac GFP (IPTG+)Plac GFP (-)plac Barnase GFP (IPTG+)plac Barnase GFP (-)
Fig. 2 growth curve repressed by barnase
![Page 57: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/57.jpg)
Barnase repressed the growth of E. coli as we expected.
0
0.005
0.01
0.015
0.02
0.025
Plac (IPTG-) Plac (IPTG+) plac Barnase(IPTG-)
plac Barnase(IPTG+)
Gro
wth
Rat
e [/
min
]
Fig. 3 growth rate repressed by barnase
![Page 58: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/58.jpg)
c. Activity of Colicin Lysis Protein
![Page 59: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/59.jpg)
Colicin Lysis Protein work together with ColicinE3 and help it secret from E. coli.
![Page 60: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/60.jpg)
Colicin Lysis Protein worked well in E. coli.
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
2
0 1 2 3 4 5 6 7 8
OD
60
0
Time [hr]
Plac GFP (IPTG+)Plac GFP (-)Plac ColL (+)Plac ColL (-)
Fig. 4 growth curve repressed by Col L
![Page 61: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/61.jpg)
The way to achieve our goal.
a. Diffusion rate of E. coli
b. Strength of growth inhibition
c. Activity of Colicin Lysis Protein
![Page 62: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/62.jpg)
![Page 63: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/63.jpg)
Discussion
![Page 64: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/64.jpg)
![Page 65: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/65.jpg)
Policy and Practice: Public Engagement
May Fes.Techno Edge
![Page 66: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/66.jpg)
Policy and Practice: Survey
![Page 67: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/67.jpg)
![Page 68: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/68.jpg)
New Parts : CheZ generator (BBa_K1631018-20)
Parts Characterization: Plac Barnarse –GFP(Bba_K1631012)
Parts Improvement (BBa_I7162111→BBa_K1631000)
Policy and Practice
- Public Engagement
- Intellectual Property and Synbio
![Page 69: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/69.jpg)
References
[1]Turing, A. M. (1952). The chemical basis of morphogenesis. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 237(641), 37-72.
[2] Blanchard, A. E., Celik, V., & Lu, T. (2014). Extinction, coexistence, and localized patterns of a bacterial population with contact-dependent inhibition. BMC systems biology, 8(1), 23.
![Page 70: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/70.jpg)
AdvisorDr. James EllingerDr. Claig Hamilton
Laboratory, AdviseYouichi Wakamoto, a professor of SynBio, from University of Tokyo
OverallMother of Mr. Nakamura for her warm heartLecture room, University of Tokyo
![Page 71: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/71.jpg)
Policy and PracticeInterviewHiroyuki Takahashi, from Leave a Nest corp. Youichi Muratomi, a professional of Intellectual Property,
from Yokohama National University Many Students from all over the world
OverallGerardo AugusutoUrbina Sánchezguests of May Festival
![Page 72: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/72.jpg)
![Page 73: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/73.jpg)
![Page 74: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/74.jpg)
Our attribution page can’t be seen from Judging Form
VISIT: http://2015.igem.org/Team:UT-Tokyo/Attribution
or click “Attribution” icon from our wiki top page!!
THANKS FOR YOUR COOPERATION
Team UT-Tokyo 2015
![Page 75: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/75.jpg)
![Page 76: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/76.jpg)
![Page 77: PowerPoint プレゼンテーション2015.igem.org/files/presentation/UT-Tokyo.pdf · 2015. 10. 8. · Policy and Practice Interview Hiroyuki Takahashi, from Leave a Nest corp. Youichi](https://reader034.vdocuments.mx/reader034/viewer/2022051606/6029d0ef76b8cd48ba73888c/html5/thumbnails/77.jpg)