polyclonal antibody p5cs - proteintech · 2020-02-21 · selected validation data mcf7 cells were...
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![Page 1: Polyclonal ANTIBODY P5CS - Proteintech · 2020-02-21 · Selected Validation Data MCF7 cells were subjected to SDS PAGE followed by western blot with 17719-1-AP(P5CS antibody) at](https://reader031.vdocuments.mx/reader031/viewer/2022041116/5f284a5ecc9f4363975d8087/html5/thumbnails/1.jpg)
P5CS Polyclonal ANTIBODYCatalog Number:17719-1-AP Featured Product 4 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:17719-1-AP
Size:150UL ,200 μg/mL
Source:Rabbit
Isotype:IgG
Purification Method:Antigen affinity purification
Immunogen Catalog Number:AG12100
GenBank Accession Number:BC106054
GeneID (NCBI):5832
Full Name:aldehyde dehydrogenase 18 family,member A1
Calculated MW:795aa,87 kDa
Observed MW:87 kDa
Recommended Dilutions:WB 1:500-1:2400 IP 0.5-4.0 ug for IP and 1:500-1:1000for WBIHC 1:50-1:500
Applications Tested Applications:IHC, IP, WB, ELISA
Cited Applications:WB
Species Specificity:human, mouse, rat
Cited Species:human
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : MCF7 cells; HeLa cells, HepG2 cells, mouse braintissue
IP : mouse brain tissue;
IHC : human breast cancer tissue;
Background Information Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and theNAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolicprecursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the causeof cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternativesplicing.
Notable Publications Author Pubmed ID Journal Application
Wei Liu 26598224 Sci Rep WB
Ling Guo 30783087 Nat Commun WB
Robert B Hamanaka 30973753 Am J Respir Cell Mol Biol WB
Storage Storage:Store at -20°C.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
![Page 2: Polyclonal ANTIBODY P5CS - Proteintech · 2020-02-21 · Selected Validation Data MCF7 cells were subjected to SDS PAGE followed by western blot with 17719-1-AP(P5CS antibody) at](https://reader031.vdocuments.mx/reader031/viewer/2022041116/5f284a5ecc9f4363975d8087/html5/thumbnails/2.jpg)
Selected Validation Data
MCF7 cells were subjected to SDS PAGE followedby western blot with 17719-1-AP(P5CS antibody) atdilution of 1:500 incubated at room temperature for1.5 hours
IP Result of anti-P5CS (IP:17719-1-AP, 3ug;Detection:17719-1-AP 1:500) with mouse braintissue lysate 5000ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using17719-1-AP( P5CS antibody at dilution of 1:200(under 10x lens). heat mediated antigen retrievedwith Tris-EDTA buffer(pH9).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using17719-1-AP( P5CS antibody at dilution of 1:200(under 40x lens). heat mediated antigen retrievedwith Tris-EDTA buffer(pH9).