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Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke Moe (NIVA) deWELopment project meeting 10.02.2011, Warszaw

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Page 1: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Estimation of uncertainty in status class assessment

for Wel waterbodies

Jannicke Moe (NIVA)

deWELopment project meeting

10.02.2011, Warszaw

Page 2: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

From the deWELopment project description:

Third phase: from single metrics to BQE-level assessment

• We will test different methods of combination of these single metric results to obtain a total result at the whole element level, taking into account the uncertainty in the different single metrics

• In our project we will test alternative approaches [ to the one-out-all-out principle] using different methods– simple averaging, weighted averaging, multimetric approach

Page 3: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

From the deWELopment project description:

Fourth phase: from BQE-level to waterbody-level assessment

• Testing different ways of combining the assessment results for different BQEs into one final result for the whole waterbody. – Here the recommended by one-out-all-out rule will be compared

to other alternative methods.

• The risk of misclassification will be estimated– software STARBUGS ( WISERBUGS)

Page 4: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Outline

• Uncertainty and risk of misclassification at BQE level

• Integration of uncertainty from BQE level to waterbody level

• WISERBUGS tool: examples with deWELopment results

• Next steps

Page 5: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Uncertainty and risk of misclassification at BQE level

Page 6: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Uncertainty: "accuracy" vs. "precision"

• High accuracy, but low precision– "roughly right"

• High accuracy and high precision– optimal result

• Low accuracy, but high precision– "precisely wrong"

metric

True value

metric

True valuemetric

True value

5

5

5

• We can never know the ”true value”of a BQE metric – only the measured value

• Standard Deviation (SD) is a measure of precision, not accuracy

Page 7: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Uncertainty in BQE level

• If we can never know the ”true value” of a BQE metric – how can we say something about uncertainty???

• We must assume that the measured mean value represents the true value and the true status class

• We can assume that measured metric values follow normal distribution due to sampling uncertainty

• We can let measured SD represent sampling uncertainty

Example:Measured metric values: 3, 5, 5, 6, 6Mean: 5Standard Deviation: 1.22

Histogram of DATA

De

nsi

ty

0 2 4 6 8 10

0.0

0.2

0.4

0.6

0.8

BQE metric value0 2 4 6 8 10

Page 8: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Risk of misclassification - BQE level

• With the given mean and SD, we can test: if we re-sample the BQE many times, how often will we get the ”true” status class?

• Assuming that measured status class = true status class

0 2 4 6 8 10

0.0

0.2

0.4

0.6

0.8

BQE metric value

pro

ba

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sity

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meanSD = 1.22

p=0.7% p=20% p=58.6% p=20% p=0.7% Risk of misclassification:

= proportion of ”new samples” which result in wrong status class

= 0.7% + 20% + 20% + 0.7%

= 41.4%

Page 9: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

0 2 4 6 8 10

0.0

0.2

0.4

0.6

0.8

BQE value

pro

ba

bili

ty d

en

sity

fun

ctio

nmean

SD = 1.5

p=2.3% p=23% p=49.5% p=23% p=2.2%

Risk of misclassifcation increases with SD

- Higher SD gives flatter distribution=> higher probability that new BQE values fall outside the true class

Example:

- SD increases from 1.0 to 1.5

- Probability of misclassification increases from 32% to 50%

- ”True class” is Moderate, but 25% probability that new samples will result in Good or High

0 2 4 6 8 10

0.0

0.2

0.4

0.6

0.8

BQE value

pro

ba

bili

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meanSD = 1

p=0.1% p=15.7% p=68.3% p=15.7% p=0.1%

Page 10: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Risk of miscl. increases near class borders

- If the measured mean BQE is close to a class border, then new BQE values are more likely to fall into a neighbour class

Example:

- Mean BQE decreases from 5 to 4.2

- Probability of misclassification increases from 32% to 46%

- ”True class” is moderate, but 42% probability that new samples will result in Good or High

0 2 4 6 8 10

0.0

0.2

0.4

0.6

0.8

BQE value

pro

ba

bili

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en

sity

fun

ctio

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meanSD = 1

p=0.1% p=15.7% p=68.3% p=15.7% p=0.1%

0 2 4 6 8 10

0.0

0.2

0.4

0.6

0.8

BQE value

pro

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ctio

nmean

SD = 1

p=1.4% p=40.7% p=54.3% p=3.6% p=0%

Page 11: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

How can we obtain SD for BQE level?

• Can we get reliable SD estimates from deWELopment data?– Many samples are needed per BQE and waterbody –

we have ”only” 1-2 samples per BQE and waterbody (?)– Metric values are not necessarily normally distributed

• Other distributions can be considered

• What are the alternatives?– ”Use best-available information from replicated sampling studies

on environmentally similar waterbodies” (WISER data?)– Use best guesses– (Ask WISER WP6.1 for advice)

Page 12: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Integration of uncertainty from BQE level to waterbody level

Page 13: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Macroinvertebrates

Phytobenthos

Hydrology

Acidification

Organic

Combining metrics and BQEs

Page 14: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Two issues:1) How to combine status classes for different metrics and BQEs

• Average; weighted average; all-out-one-out; etc. • Will not be discussed here (see my presentation June 2010)

2) How to combine uncertainty from different metrics and BQEs • Tool: WISERBUGS

Combining metrics and BQEs

Page 15: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

WISERBUGS – brief introduction

• WISER Bioassessment Uncertainty Guidance Software

• Excel-based tool developed within EU project WISER

• Purpose: assist in quantifying uncertainty in the assessment of ecological status of waterbodies

• Can be used for testing impact on classification of:– Combination rules for metrics and for BQEs– Class boundaries and reference conditions– Sampling uncertainty – SD (per metric)– Sorting/identification uncertainty – SD (per metric)– Uncertainty in reference condition – SD (per metric)

• Can not be used for – estimating SD for metrics (must be done separately)– estimating type I/II errors (because true status class is not known)

Page 16: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

WISERBUGS – brief introduction

WISERBUGS output: probability of each status class for...

• each waterbody (overall assessment)

• each BQE within a waterbody

• each metric within a BQE

High Good Moderate Poor Bad

0.0

0.2

0.4

0.6

0.8

Status class

pro

ba

bili

ty

Page 17: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

WISERBUGS – brief introduction

WISERBUGS input

• For each metric:– Measured value for each sample in each waterbody– SD representing sampling variation– Class boundaries (H/G, G/M, M/P, P/B)– ”E1”: metric value for which EQR = 1 (Reference value)– ”E0”: metric value for which EQR = 0 (bottom of metric scale)– (SD representing sorting/identification variation)– (SD for reference value)

• For overall assessment:– Combination rules for metrics within BQE– Combination rules for BQEs within waterbody– (Correlation between metrics)

Page 18: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

WISERBUGS tool: examples with deWELopment results

Page 19: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Data received from deWELopment

• Selected metrics:– Rivers:

• MP: River Macrophyte Index

• PB: Multimetric Diatom Index for rivers

• MI: Benthic Macroinvertebrate Index

• FI: European Fish Index +

– Lakes: • PP: Chlorophyll a; Phytoplankton Metric for Polish Lakes

• MP: Ecological State Macrophyte Index

• PB: Diatom Index for Lakes

• MI: Benthic Quality Index based on Chironomid Pupal Exuvial Techn.

• FI: Fish Index 'Summ Best’ (no class boundaries yet)

Page 20: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Data received from deWELopment

• Metric values:– 13 rivers + 10 lakes– Usually all BQEs for each waterbody

• Standard deviations per metric: not available– Randomised values used for this excercise– (To be discussed)

• Class boundaries and reference conditions (”E1”) per metric:– Sometimes waterbody-specific (OK for WISERBUGS)

• ”E0” – given in correct scale?

Page 21: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Metric specification - 1 (Lakes)

Class boundaries

Metric names

Other details

Page 22: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Metric specification - 2 (Lakes)

Other types of variation

SD from sampling variation

NB: SD values are made up!

Page 23: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Metric specification - 3 (Lakes)

Grouping of metrics by pressure within BQE

Grouping of metrics by BQE

Page 24: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Metric specification - 4 (Lakes)

Weighting of each BQE

Rule for combining BQEs within waterbody(here: one-out-all-out)

Rule for combining metrics within BQE

Page 25: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Results: assessment for individual metrics

NB: Fake SD values - results must not be interpreted as real.

Page 26: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Results: assessment combined per BQE and per waterbody

• Combination rule for total assessment: ”worst case” (all 4 BQEs)

Page 27: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Results: assessment for BQEs and waterbody

• Combination rule for total assessment: ”average” (all 4 BQEs)

Page 28: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Next steps

Page 29: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Next steps for data analysis

• Try to obtain SD estimates / best guesses from BQE groups

• Quality-check of class boundaries, E0, E1 etc. – Confusion EQR scale vs. original metric scale?

• Explore impact on classification of...– different level of uncertainty (sampling SD)– different combination rules– etc.– what is most useful for deWELopment?

• Estimate risk of misclassification for selected cases– ”True class” will be determined by the given metric values,

and an agreed set of SD and combination rules

• Include physico-chemical parameters in assessment?

• Other suggestions?

Page 30: Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund Estimation of uncertainty in status class assessment for Wel waterbodies Jannicke

Polsko-Norweski Fundusz Badań Naukowych / Polish-Norwegian Research Fund

Publication

• 1 manuscript on exploring risk of misclassification using the metrics and classification system developed by deWELopment and the WISERBUGS tool

• Potential co-authors– BQE group leaders ? – Gosia ? – Coordinators: Hanna and Anne (or acknowledgement?)– WISERBUGS author: Ralph Clarke (or acknowledgement?)