platform for the hypoxic analysis of cell behaviour (hyp-acb) · [email protected]...

Main Applicant Francesca Cutruzzolà (Dept.Biochemical Sciences-DSB)

Co-Applicants Andrea Bellelli (DSB), Rita Mancini (Dept. Clinical and Molecular Medicine, DMCM)

Platform for the Hypoxic Analysis of Cell Behaviour (Hyp-ACB)

Call 2016

HYPEROXIA HYPOXIA

Cellbehaviourdependsonenvironmentaloxygen

Oncology Immunity and inflammation Neurobiology Regenerative medicine Host-Pathogen interaction Drug discovery Toxicology Environmental biology

Re-shapingCELLULARMETABOLISM

andGENEEXPRESSION

PHYSIOXIA


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Cellular Metabolism Gene expression

LIVING CELLS Oxygen Consumption rate

Glycolitic activity ATP production Carbon source

(glucose, glutamine, fatty acids)

Core Checkpoint Gene Set

(CCGS) hif, idh, shmt, myc…..

Specific Gene Set (SGS) Custom-made

Biologicalsample

pO2(20,10,5-3%)


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THEAPPLICATION

SupportingStaffMembers:52members

8DepartmentsInvolved:

ScienzeBiochimicheBiologiaebiotecnologieDarwinMedicinaclinicaemolecolare

MedicinamolecolareScienzeebiotecnologiemedico-

chirurgicheMedicinaSperimentaleBiologiaAmbientale

Fisiologiaefarmacologia

THELAB•  35m2dedicatedrenovatedlab

•  DepartmentofBiochemicalSciences(maincampus)buildingCU027,roomsS32/S33(groundfloor).

THEPERSONNELProf.FrancescaCutruzzolà(FullProfessor-ManagingDirectorandScientificOfficer)Prof.SerenaRinaldo(AssociateProfessor-DataanalysisManager)Dr.AlessioPaone(RTD-A–Cellbiologist)Dr.GiovannaBoumis(Technician–technicalsupport)

NUMBERSANDFACTS


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CellBiology

SeahorseanalyserDedicatedHypoxicchamber

MolecularBiology

UNIQUE IN ITALY


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Exp-1 Exp-2 Exp-3 Exp-4

ACCESS to the Equipment

ASSISTANCE with operation

SUPPLY reagents and materials

METHOD DEVELOPMENT

SELECTION of methodology

EXPERIMENTAL design

DATA storage

METABOLICEXPERIMENTFLOWCHART


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TECHNICALREPORT

REPORTS

DETAILEDSCIENTIFICREPORT


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Biologicalsamples:examplesfromongoingexperiments

Cancer cell lines (Leukemias, Lung adenocarcinoma,

Prostate and Cervical cancer..)

Normal eukaryotic cells (Fibroblasts, prostate cells

microglia)

Bacteria

Neurospheres (neural stem cells)


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DRUGS

TRANSFECTIONS

DIFFERENTIATION

STIMULATION WITH COMPOUNDS

•  STEP1:Accessisrequestedbye-mailtotheManagingdirectororadelegate(seecontacts).Experimentalbackground,numberofsamplestobeassayed….

•  STEP2:EvaluationoftherequestbytheHypACBteam.Criteria:

Feasibilityoftheexperimentandtypeofaccessrequired.Scheduledpriority: Level1:Internalacademicusers. Level2:Externalacademicusers. Level3:Externalnon-academicinvestigators.

• STEP3:Experimentisrunbytheplatformpersonnel.

Theresultsoftheexperimentswillbeprovidedwithallexperimentaldetails[…].Rawdatawill be stored as a copy for 5 years, according to international rules for scientificcorrectness.Dataanalysiscanbeperformedbytheend-usersorbytheHypACBteambasedonspecificagreement.

USERPOLICIESANDGUIDELINES


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•  STEP1:Accessisrequestedbye-mailtotheManagingdirectororadelegate(seecontacts).Experimentalbackground,numberofsamplestobeassayed….


Feasibilityoftheexperiment.Scheduledpriority: Level1:Internalacademicusers. Level2:Externalacademicusers. Level3:Externalnon-academicinvestigators.

• STEP3:Experimentisrun.TypeofaccessbytheHypACBpersonnel.

AcknowledgementsInvestigatorsarerequiredtoacknowledgetheHyp-ACBplatform(andSapienzaFinancialContributionbyGrantNumber)inanypublicationthatresultsfromtheuseoftheplatform.Namesofindividualsfromplatformwillbeincluded,ifpreviouslyagreedoriftheyprovidedanyintellectualinputoradditionaleffort.


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•  STEP1:Accessisrequestedbye-mailtotheManagingdirectororadelegate(seecontacts).Experimentalbackground,numberofsamplestobeassayed…..


Feasibilityoftheexperimentandtypeofaccessrequired.Scheduledpriority: Level1:Internalacademicusers. Level2:Externalacademicusers. Level3:Externalnon-academicinvestigators.

• STEP3:TypeofaccessisagreedandExperimentisrun.

AcknowledgementsInvestigatorsarerequiredtoacknowledgetheHyp-ACBplatform(andSapienzaFinancialContributionbyGrantNumber)inanypublicationthatresultsfromtheuseoftheplatform.Namesofindividualsfromplatformwillbeincluded,ifpreviouslyagreedoriftheyprovidedanyintellectualinputoradditionaleffort.



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•  STEP1:Accessisrequestedbye-mailtotheManagingdirectororadelegate(seecontacts).Experimentalbackground,numberofsamplestobeassayed……..


Feasibilityoftheexperiment.Scheduledpriority: Level1:Internalacademicusers. Level2:Externalacademicusers. Level3:Externalnon-academicinvestigators.

• STEP3:TypeofaccessisagreedandExperimentisrun.Resultsareprovidedandstored.

AcknowledgementsInvestigatorsarerequiredtoacknowledgetheHyp-ACBplatform(andSapienzaFinancialContributionby

GrantNumber)inanypublicationthatresultsfromtheuseoftheplatform.Namesofindividualsfromplatformwillbeincluded,ifpreviouslyagreedoriftheyprovidedany

intellectualinputoradditionaleffort.



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TYPEOFACCESS

Directaccessbyendusers

Experiment(fromcell

culturetodata)

Fullexperimentalplanningandfollow-up


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TimeallocationTime

MaterialsPersonnel

AnalysisInterpretation

Methoddevelopment

TYPEOFACCESS


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TimeallocationTime

MaterialsPersonnel


Methoddevelopment

150€ 1200€

OFFICIAL PRICE LIST 2019 UNDER APPROVAL

+35%forhypoxia

ExampleofExperimentalCosts(Metabolism96-wellplate)

TYPEOFACCESS:Serviceonly


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FOR SAPIENZA USERS: COST REDUCTION IS PLANNED (APPROX. 30-50% )

TimeallocationTime

MaterialsPersonnel


Methoddevelopment

150€ 1200€

OFFICIAL PRICE LIST UNDER APPROVAL

+35%forhypoxia

ExampleofExperimentalCost(Metabolism96-wellplate)

TYPEOFACCESS:Collaboration

CHARGE REDUCTION


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Accessonly Experimentandaccess Full

TYPEOFACCESS:Training


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NEW REQUESTS WELLCOME!

TEMPORARY WEBSITE www.macinmec/hypacb-facility/


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CONTACTS

•  Managing director: prof. Francesca Cutruzzolà [email protected] Tel. 0649910955

Platform Staff

Data Manager: Prof. Serena Rinaldo [email protected]

Cell Biology: Dr. Alessio Paone [email protected]

Technical staff: Dr. Giovanna Boumis [email protected]


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platform for the hypoxic analysis of cell behaviour (hyp-acb) · [email protected]...

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