platelet function testing
DESCRIPTION
Platelet Function Testing. Kit for each patient The packet contains everything needed. We will now demonstrate how to perform the test. Three platelet stimulant tubes. Instruction leaflet Citrate blood collection tube. aspirin test clopidogrel test baseline test. insulated pouch - PowerPoint PPT PresentationTRANSCRIPT
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Platelet Function Testing
Kit for each patient
The packet contains everything needed
We will now demonstrate how to perform the test
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insulated pouchheating padconnecting 2way adaptorsfixing solution
aspirin testclopidogrel testbaseline test
Instruction leafletCitrate blood collection tube
Three platelet stimulant tubes
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Activate the heating pad by clicking on the circular valve inside
Warming the tubes
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When activated the gel turns white and warms up immediately
Warming the tubes
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Place the heated pad inside one pocket of the insulation pouch
Warming the tubes
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Place the three stimulant tubes in the other pocket of the insulation pouch
Warming the tubes
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The stimulant tubes are now ready to use
Warming the tubes
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Venepuncture
Select the green topped citrate monovette
Blood should preferably be collected using one of the following venepuncture procedures
ButterflyBD VacutainerSarstedt Multifly set
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Butterfly you will also need the Sarstedt multi-adaptor valve
Venepuncture
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BD Vacutainer Safety-Lok you also need the Sarstedt multi-adaptor valve
Venepuncture
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the Sarstedt multi-adaptor valve
Venepuncture
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Sarstedt MultifySet already supplied with the adaptor for the monovette tube
Venepuncture
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Filled citrate anticoagulant tube
Fill to the 10 ml line by pulling gently on the plunger (do not lock off the tube)Invert 3 times to mix the blood
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Pull the plunger pulled back (do not lock off the tube)Add the blood from the syringe up to the 10 ml markScrew on the green capInvert 3 times to mix the blood
If you have to use a needle and syringe
If using a needle and syringe the tube will need to be opened by holding the monovette upright and unscrewing the green end taking care not to spill the anticoagulant inside.
10 ml mark
Venepuncture
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Filled citrate anticoagulant tube
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Place the blood in the insulation pouch with the stimulant tubesuntil ready to perform the test
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Mix the blood and attach one of the 2way adaptors
Platelet stimulation
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Attach each stimulant tube and add blood in turn
Fill three stimulant tubes
Platelet stimulation
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To fill, pull back plunger until top of the plunger aligns with bottom arrow
Blood should flow up to4.5 ml mark
Platelet stimulation
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Place the stimulant tubes back in the pouch to keep warm
Check the time and wait 5 minutes!
Platelet stimulation
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While waiting for the 5 minutes
Discard the used citrate tube and adaptor valve
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While waiting for 5 minutes
Take the second 2way adaptor and attach to the Fixing Solution Tube
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When 5 minutes is passed
Attach each stimulant tube in turn to the 2way adaptor Pull back plunger all the way and fill the tubes with the fixing solution (liquid aligns with the 4.5 ml mark)
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Completed stimulant tubes
Invert to mix
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Snap off the plungers on the stimulant tubes
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Write patient details on the labels on each of the three stimulant tubes
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Place the completed stimulant tubes in the padding for transportation
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Place tubes inside the packageand transfer pack to Nottingham
Platelet functionwill be measured asplatelet P-selectin expression