pl01b-pwtsew-efl-bal e˜ochem potable w ater / …[email protected] eflochem chemicals • p.o....

EFLOCHEM CHEMICALS • P.O. BOX# 12282 • DUBAI-UAETEL:# +9714 3472900 • FAX# +9714 3477072 • [email protected] • www.eflochem.com

MANUAL

PL01B-PWTSEW-EFL-bal

E�ochem Potable W ater / Sewage / Ballast Water Kit

TEST

How to dilute samples to extend test range

Chloride Tablet Count Methode

Permanganate Value (PV/BOD/COD/TOC)

Electronic Meter pH/Temperature

Electronic Meter Electronic COnductivity (EC)

E.Coli and Pseudomonas Aeruginosa / TTC Bacteria Test

Pseudomonas Species and Coliform Bacteria Test

Bacteria Test Dipslide Incubator

Enterolert Enterococci Test

Diagnose Cholera Test

PrimeLab 1.0 Photometer

PrimeLab - Turbidity Adapter

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FILTRATION

While conducting water testing, there are cases in which collected samples may be turbid. This complicates the end point color & the results may not be correct. In such cases, filtration of the turbid sample with a special filter paper Is necessary. After filtration it may be necessary to dilute the sample, if it is still turbid, by adding distilled water (DW). The result of the test is multiplied by the corresponding factor which is the dilution factor, as given below.

Sample Vol.(ml)

DW Vol.(ml)

Dil Factor

WATER SAMPLING POINTS

Feedwater: From main feedline (as close as possible the boiler).

Boiler: From Boiler Blowdown, as instructed by the manufacturer. Sample should be cooled to < 26° C. Sampling Procedure: Flush sampling line a few minutes then reduce the flow for 3-5 minutes with no disturbance. Flush the sample bottles a few times with the sample water. Collect the sample in the bottle w/o touching the samplingline. If the analysis is to be delayed, sample must be kept in tightly closed sample bottles.

< 28 Bar28 - 56 Bar

&

/ TDS

Oxygen Scavengers

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-1. Use 2 ml to measure range 500 - 5000 mg/l Cl or 800 - 8000 mg/l NaCl- Use 10 ml to measure range 100 - 1000 mg/l Cl or 160 - 1600 mg/l NaCl

- Use 50 ml to measure range 20 - 200 mg/l Cl or 32 - 320 mg/l NaCl- Use 100 ml to measure range 10 - 100 mg/l Cl or 16 - 160 mg/l NaCl

2. Add one tablet of Chloride with gentle mixing. The sample should turn yellow.3. Add tablets of Chloride one at a time with gentle mixing until the brown colour disappears. Count the number of tablets used.

Determination of Chloride-Cl (10 - 5000 mg/l)

NaCl (16 - 8000 mg/l)

SVZ1600Kit contains:

Black plastic carrying case100 ml shaker tube (SVZdev100)

Stirring rod (SPstr1)Cleaning brush (Spclb1)

250 tablets Chloride (TbsRCD250)

Attention!Wear protective gloves and safety gogles when performing any tests using corrosive, harmful or irritant reagents. Do not ingest.


-Clmg/l

NaClmg/l

mg/l 1 1.6

Conversion factors

-Cl NaCl

2 ml 10 ml 50 ml 100 ml 2 ml 10 ml 50 ml 100 ml

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2 500 100 20 10 800 160 32 16

3 1000 200 40 20 1600 320 64 32

4 1500 300 60 30 2400 480 96 48

5 2000 400 80 40 3200 640 128 64

6 2500 500 100 50 4000 800 160 80

7 3000 600 120 60 4800 960 192 96

8 3500 700 140 70 5600 1120 224 112

9 4000 800 160 80 6400 1280 256 128

10 4500 900 180 90 7200 1440 288 144

11 5000 1000 200 100 8000 1600 320 160

N° of tablets

Results

-20 - 70 mg/l Cl : Maintain daily blowdown!-100 - 220 mg/l Cl : Acceptable chlorides in low pressure boiler!-240 - 400 mg/l Cl : Reduce chlorides by increased blowdown!

Note!If measuring boiler water, it is possible to use the completed Alkalinity test sample, then follow instructions from step 2 above.

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PERMANGANATE VALUE (PV)(BOD / COD & TOC)

The Permanganate Value is determining the general quality of Final Effluents in sewage. Based on the results it will give the OK for the discharge of Sewage water. The test procedure is relatively easy and consists of 4 steps:

Ÿ Three sample containers in the set must be filled with 100 ml of Sewage Effluent.Ÿ Add two Acidifying SE tablets per sample container and shake it to mix well.Ÿ Add one permanganate value tablet to the first sample container, then add two permanganate tablets to the second and three tablets to the third, and again shake them well until it all of them are mixed and dissolved.Ÿ Wait 30 minutes! Then check how many tubes have remained pink and read the result from the following table:

Container Pink Permanganate Value Grading

3

2

1

None

0 - 10

10 - 20

20 - 30

30 or more

Perfect

Satisfactory

Dubious

Not allowed to discharge

In the Test Kit you will also find instructions for the testing of Crude waste, the same is valid for Settled sewage.

BOD / COD & TOC

BOD = Biochemical Oxygen Demand COD = Chemical Oxygen Demand TOC = Total Organic Carbon

The results from this test are in connection with the Permanganate Value (PV) as done in paragraph1. to convert the PV for domestic sewage and effluent to probable BOC, COD and TOC values multiplyby the following factors:

Sewage Effluent

Probable BOD

Probable COD

Probable TOC

PV x 5

PV x 10

PV x 3

PV x 1,5

PV x 7

PV x 2

In general there is a connection between the Turbidity and the BOD-value of settled sewage and efluent. The BOD can also be calculated from the result of the turbidity test usig the fomula:

Probable BOD = + 5Turbidity

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BOD-values can be cross checked that way with the PV tests. Recommendation for Effluent is not more than 20 mg/l.

Kit contains:3 x 100 ml shaker tube (SVZdev100)

Stirring rod (SPstr1)Cleaning brush (SPclb1)

200 tablets Acidifying SE (TbsHASE200)100 tablets Permanganate Value (TbsPPMGNV100)

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Electronic Meter FT15pH 0.00 - 14.00(0.0 - 50.0 °C)

Ÿ 1 point manual calibration (pH)Ÿ Incl. 4 x 1.5 V batteriesŸ In a dark blue plastic caseŸ English manual

Operation:

Make sure the tester is calibrated to pH 7.001) Remove protective cap and switch meter on.2) Immerse electrode in solution to be tested.3) Stir gently and wait for the reading to stabilize.4) Press “°C/°F“ to switch between Celsius and Fahrenheit.5) Read the result, than rinse the electrode with water.6) Replace the protective cap and switch the meter off.7) Make sure the electrode is kept wet all the time!

Buffer solution:

20 ml sachet / 7.00 pH / 1-time-use


Operation:Calibration:

Battery:

FT15 “7.00 PH“

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Electronic Meter FT35EC / Electronic conductivity: 0.00 - 19.99 mS/cm

Ÿ 1 point manual calibrationŸ Incl. 4 x 1.5 V batteriesŸ In a leather like bag with belt clip

Operation:Calibration:

Battery:


Operation:

Make sure the tester is calibrated to 12.88 mS/cm1) Remove protective cap and switch meter on.2) Immerse electrode in solution to be tested.3) Stir gently and wait for the reading to stabilize.4) Read the result, than rinse the electrode with water.5) Replace the protective cap and switch the meter off.

Buffer solution:

20 ml sachet / 12.88 mS/cm / 1-time-use

4FT35 „12.88 mS/cm“

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DipslidesSamling & Testing Notes

Weekly monitoring of bacteria levels has been recommended by many legislators and professional authorities as a visual performance indicator to both system and treatment regime. This allows theuser to gauge how effective a chemical or biocide product is in the particular application and a trend can be quickly established identifying changes and taking quick remedial action where required. It should be noted from the outset that Dipslides alone do not detect Legionella as a select micro-organism, however it is generally accepted that overall bacteria levels in excess of 10“ are considered able to support Legionella and obviously a serious risk.

The dipslide consists of a plastic paddle with culture media on each side, the tube keeps the media both moist & sterile until required. The product most suitable is a slide based on a standard nutrient agar with a red dye supplement added during production, this has the advantage of showing any viable colonies as red dots, easily identified and compared against the comparison chart.

IMPORTANT - Monitoring is not a substitute for a treatment regime, it is always best to seek the advice of a professional water treatment or environmental company who will supply a risk assessment together with the required treatment system. The advantage of your weekly monitoring program is you can see how well the system is performing and identify any problems in house in-between visits. Counts should never exceed 10‘ at any time.

1 - Prior to use please keep the slides in a cool place (not a fridge) at around 10 - 15°C.

Dipslides have a typical shelf life of 8 - 9 months. Once the dipslide is opened care must be taken not to touch the media or expose the media to the atmosphere in order to prevent false contamination.

2 - Ideally the sample should be taken in a clean container rinsed with the water to be tested.

You can also sample directly from the tower sump ensuring you do not touch any of the surfaces. Submerge the dipslide to the top of the culture media for around 2 seconds and then shake gently to remove excess fluid replacing in the tube.

3 - Place the slide into the Incubator, the correct temperature is 30°C for a period of 48 hours.

Incubation is vital for accurate results. The dipslides should only be incubated in an incubator. If you are mobile you must ensure you incubate the dipslides in a dual voltage incubator which will operate in a vehicle.

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DipslidesApplication, Storage & Disposal

StorageDipslides should be stored in cool dry conditions, but not in a refrigerator - prior to despatch ourstock is stored in a cold room at 10'C in order to prevent condensation and de-hydration of theagar and ensure Dipslides arrive in perfect condition.

Shelf LifeDipslides have a typical use by date of 9 months dependent of manufacture cycle, they can beused after this date as long as no contamination or visible shrinkage shows on the agar surface -excess water in the bottom of the slide would indicate the storage temperature was too high.

ApplicationDipslides can be used to monitor microbial growth wherever the potential may exceed 1000 (101)organisms per ml of sample fluid. Industrial Waters: For detection of slime forming bacteria in cooling & industrial waters, storage tanks and for evaluating biocide perfomiance in treated systems.

Industrial Fluids: For detection of bacteria & moulds in metal-working fluids, paper processing waters, fuel tanks etc.

Environmental Hygiene: To monitor the surface contamination within domestic or institutional sites.

Leather Industry: For detection of spoilage organisms in hide and skin soaking liquors.

Product Safety - MSDSDipslides when new are non-hazardous.

Disposal of Used SlidesContaminated slides should be sterilised before disposal by immersing in a disinfectant or byautoclave or incineration. After soaking in a disinfectant for 24 hours the dips can be disposed ofin any normal non hazardous waste service.

Quality ControlDipslides are manufactured in a clean environment and stored at constant temperature prior todespatch. All materials and each batch are traceable from source to our customer.

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Organism

Colony Size (mm)

Shape & Surface

Colour

Comments

Escheria Coli

1.0 to 2.0

CV.E.G

Blue-Purple

Enterobacter Aerogenes 1.5 - 2.5 C.VE.G Rose Pink

Pseudomonas Aeruginosa 0.5-1.0 FED/C.VE.G Buff Enterococcus faecalis No growth

Staphylococcus aureus

No growth

CV.E.G = Convex entirely glossy, FED = Full entire dull



Bacteria Testing BactD005E.Coli, Coliforms, Pseudomonas Species / TTC (Total Viable Count)

Ÿ 10 E.Coli / TTC Dipslide TestsŸ Flexible paddle for effective surface contact

The E.Coli/TTC dipslide allows convenient enumeration of aerobic bacteria (TVC) together with E.Coli, Coliforms and Pseudomonas bacteria in a single test. The dipslide is prepared with Nutrient TTC agar on the lighter side (responsive to aerobic bacteria) and a chromogenic agar on the darker side. The nutrient agar reacts with enzymes to produce a colour change which is specific to the bacteria type, allowing easy enumeration.

SAMPLING: FLUIDSThe sample should be taken by immersing both sides of the paddle into the fluid to be tested, it havingfirst been removed from the sterile container. Excess sample should be gently shaken from the paddlebefore it is replaced in the container.

SAMLPLING: SURFACESThe sample should be taken by allowing direct contact between te agar surface and the test material.The paddle is flexible and can be bent at the upper end to allow both surfaces to come into intimatecontact. Bacterial recovery rate is about 50% so that sweeping an area approximately twice that of thepaddle will give a more accurate result.

INCUBATION:Incubate at 30°-35°C C for 24-48 hours, when full enumeration should be completed.

DISPOSALUsed slides should be incinerated or autoclaved. Alternatively, immerse in a 10% bleach solution for 24 hours.

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Organism

Colony Size (mm)

Shape & Surface

Colour

Comments

Pseudomonas Aeruginosa

Spreading

Glossy, Thin

Yellow-Green

PDM

P. fragi 1.0 - 3.0 CVEG Grey PDM

E. aecalis 1.0-2.0 CVEG Red MacConkey No. 3

E. coli 1.5-2.5 CVEG Pink/ Red MacConkey No.3

S. typhimurium

0.5-1.0

FED

Colourless

McConkey No. 3

CV.E.G = Convex entirely glossy, FED = Full entire dull



Bacteria Testing BactD006Pseudomonas species and Coliforms

Ÿ 10 PDM / MAC Dipslide TestsŸ For potable water, pools and spasŸ Flexible paddle for effective surface contact

The PDM/MAC dipslide allows convenient enumeration of Pseudomonas spp. on the pale strawPseudomonas differential medie (PDM) and enterobacteriaceae on the reddish/brown MacConkeyside. PDM agar is a differential media that selects for Pseudomonas spp, while MacConkey agar isdifferential, all in one test for gram negative bile tolerant enterobacteriaceae.

SAMLLING: AIRThe sample should be taken by inverting the paddle and inserting in the vial. Let it sit for 15 minutesexpodes to the air, the replace the paddle in the vial and sela tightly.

SAMPLING: FLUIDSThe sample should be taken by immersing both sides of the paddle into the fluid to be tested, it havingfirst been removed from the sterile container. Excess sample should be gently shaken from the paddlebefore it is replaced in the container.

SAMLPLING: SURFACESThe sample should be taken by allowing direct contact between te agar surface and the test material.The paddle is flexible and can be bent at the upper end to allow both surfaces to come into intimatecontact. Bacterial recovery rate is about 50% so that sweeping an area approximately twice that of thepaddle will give a more accurate result.

INCUBATION:Incubate at 30° C for 24-48 hours, when full enumeration should be completed.

DISPOSALUsed slides should be incinerated or autoclaved. Alternatively, immerse in a 10% bleach solution for 24 hours.

Surface

AEROBIC MICROBES -

TVC Fluids

0/ / / / / 0/ / / / /

MACCONKEY AGAR:Colourless: Salmonella typhimurium /Proteus mirabilis / Pseudomonas aeruginosaPink: Aerobacter aerogenes Pink/Red: Escherichia coli Yellow/Green + Buff colonies: Total coliforms

PDM AGAR:Green/Blue: Pseudomonas aeruginosaBuff/Grey colonies: Other Pseudomonas Species Green/Blue and Buff/Grey: Total Pseudomonas

Approx Colony Count per 100 ml

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Incubator BactEDT059

Ÿ Incubator for 10 dipslides and/or petri dishesŸ Applicable for Enterolert IncubationŸ Variable temperature: +5 - 45°C (+/- 0.5°C)

The BactEDT059 incubator is specifically designed for Dipslides (but is easily capable of a several 100mm Petri-dishes) and applications both on site and in a vehicle, the efficient design helps minimise drain on the vehicle battery and the robust ABS case is ideal for a life out in the field.

The BactEDT059 incubator is supplied ready for mains or 12 volt uses as standard and features our world standard plug & power adaptor - all ready to go!

Our tested MX solid state temperature control technology and heat reservoir chassis guarantee superb heat distribution without any fans or moving parts, only 1.2Kg in total weight!

Ultra VersatileFeaturing the new MX Precision Electronic Temperature Control Technology plus Worldwide Universal Power Supply with Interchangeable Power plugs for UK, Europe, and America & Australia together with Automatic voltage selection – 110 or 240 volts mains. To use in a vehicle just requires the standard & included cigar lighter cable.

Ultra SafeThe incubator runs from 12 volts DC, the mains stays at the adaptor/plug so reducing risk In situations where the Incubator could be used with liquid samples or wet hands, compliant whatever legislation brings....

Ultra ReliableMX Temperature Control Technology is fully solid state with no moving parts, element switching is transistorised and designed for constant long term operation.

Ultra AccurateTogether with our temperature control technology the BactEDT059 incubator features a heat reservoir chassis - this holds latent heat during operation adding thermal inertia to keep temperature stable even when largerloads are introduced.

Temperature RangeAmbient + 5 to 45°C +/- 0.5°C The thermometer is located on a custom ledge, safe from the load and easy to view through the polycarbonate clear internal lid, the BactEDT059 incubator is supplied with universal mains power pack and 12 volt cigar lighter cable as standard

Specification & SizePower supply – Adaptor – 100-240v AC @ 320 mAPower supply – Incubator 12 Volts DC @ 1AmpTemperature Control – MX Darlington switched solid stateTemperature Range – Ambient + 5 to 45°C +/- 0.5°CChamber Size (useable) – 11 x 13 x 70 (H) cmExternal case size- (footprint) 27 x 21 x 10(H) cmTotal Weight – Inc cables & adaptor 1.2 KgGuarantee – 2 Years

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ENTEROLERTEnterococci Test

Please refer to the manual given with the ENTEROLERT pack

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DIAGNOSE CHOLERACholera Test

Please refer to the manual given with the Diagnose Cholera pack

PrimeLab 1.0 PhotometerMulti Test Photometer

Please refer to the manual given with the PrimeLab 1.0 Photometer

PrimeLab Turbidity AdapterTurbidity Testing (NTU)

Please refer to the manual given with the PrimeLab Turbidity Adapter

pl01b-pwtsew-efl-bal e˜ochem potable w ater / …[email protected] eflochem chemicals • p.o....

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