physiology and biochemistry of ripening fruit
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Physiology and Biochemistry of
Fruit Ripening
Presented By 1) Vivek yadav
Department of Horticulture School of life Sciences
Sikkim University
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INTRODUCTION
• Ripening is the process by which fruits attain their desirable flavor, quality, color, palatable nature and other textural properties.
• Ripening is associated with change in composition i.e.
• conversion of starch to sugar.
• Change in colour
• Change in firmness
• Shape and size
• Odour /smell
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Changes occurs During Ripening
• Dev. Of wax on screen
• Flavour volatiles
• Proteins
• Organic acid
• Carbohydrate composition
• Pectic substance
• Tissue rigidity
• Ethylene
• Denaturation of chlorophyll
Ripening
Seed maturat
ion
Deg. chlorop
hyll
Abscission
Pectic sub.
Rigidity
Organic acid
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Metabolic Changes
• Increases in biosynthesis
• Evolution of ripening hormone
• Increase in respiration mediated
• Alternation of cell structure
• Hydration of cell wall
• Decrease in structural integrity
• Increase in intercellular space
In fruit ripening
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Respiratory Pattern
• Climacteric
• Non climacteric
Climacteric Non climacteric
Respiration Increase Not shows respiratory
climacteric
Ethylene More production Less amount
Detachment ripening occurs On tree only
FruitsApple,Apricot,Banana,
Guava,KiwifruitCherry,Cucumber,Grape,
Grapefruit,Lemon
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Ripening Graph Growth, respiration and ethylene production in climacteric and non climacteric fruits
Respiration rate
Ethylene production
Growth
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Mechanism of RipeningPathway of ethylene Biosynthesis and Metabolism
• Malonyl ACC• ACC synthase
SAM
SAM =S- AdenosylmethionineACC =Aminocyclopropane carboxylic acid
Yang and Hoffman 1993
At the onset of fruit ripening , expression of multiple ACC synthase genes are activated , resulting in increase production
Deamination of ACC to α- kitobutrate by over expressing ACC deaminize enzyme also inhibited ethylene formation and fruit ripening.
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Degradation of Chlorophyll and Pigment Synthesis
Chlorophyll (green)
Pheoporbide (Brown )
Chlorins, Purpurins (colourless product)
Chlorophyllin (bright green ) Phaeophytin (Olive green )
Phytol Mg++
Degradation of chlorophyll due to Chlorophyllase enzyme.
Splitting of chlorophyll into Phytol chain and porphyrin.
Loss of Mg ++ ion and conversion of porphyrin into Phaeophytin.
Change in tetrapyrolic chain and it becomes bilviridin.
Oxidation or saturation of double bonds.
Chlorophyllase Mg +
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Hydrolysis and Aroma
Enzyme Mode of action
α Amylase Mixture of glucose and maltose
Starch phosphorylase Glucose 6-phosophate
α (1-6) glucosidase Amylopectin
Starch to sugars …………
Aroma volatile after ripening …..
Product Compound
Apple Ethyle 2- methyle butyrate
Banana 2 hexanol
Lemon Citral
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Textural changes
Textural changes
Enzyme degradation of polysaccharides
Different rates i.e. Degree
Breakdown of starch
Pectic substance
Hydrolysis starch- firmness
Cell wall breakdown
PG (Polygalacturonase)
PME (Pectin methylesterase )
Other hydrolases
Cell wall Degradation
Depolymerisation Deesertification
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Phenolic Compound
S NO Crop Phenol Status
01. Grapes Flavon-3-ol monomers Decreases
02. Loquat Hydrobenzoic acid Decreases
03. Citrus Limonoids Decreases
Astringency to fruit e.g. Tannins.
Involve in oxidative browning due to Polyphenoloxidase
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Enzyme Activity
Softening –Pectin esterase
Oxidation- Catalse and peroxidase
Glycolytic action- Glucose phosphate isomerase
Hydrolytic action –Amylase cellulose, beta amylase
Pigmentation- Chlorophyllase
Many of the chemical and physical affects during ripening and after ripening processes are attributed to the enzyme action.
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Enzymatic action 05/01/2023 [email protected] 14
Unripe Fruit
Green- Chlorophyll
Hard - Pectin
Sour – Acid
Mealy- Starch
Odourless- Large orgs
Ripe fruit
Anthocyanin- Red
Less pectin- Soft
Neutral
Sugar sweet +juicy
Small orgs+odor
Hydrolase
Pectinase
Kinase
Amylase
Hydrolysis
Physical condition – Chemical cause Chemical cause-Physical condition
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Regulation of Ripening
Ethylene regulation
Regulation of O2
andCO2
• MAS• CAS
Chemical treatment • Calcium• 1MCP
methylcyclopropane
Bioregulatores • Auxin GA,CK• Ehhylene ,ABA
Ionized radiation• Gamma
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05/01/2023 [email protected] 16Thanking You