phg 461. king saud university pharmacy collage pharmacognosy depaartment lab#1 chemical &...
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PHG 461
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King Saud UniversityPharmacy Collage Pharmacognosy Depaartment
LAB#1
Chemical & chromatographic detection of A.B in milk
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N.B-:
Chemical reaction depend on color (reaction with A.B + specific reagent)
Part I
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* A.B is found in milk in small amount since it is used as animal feed additives to promote growth (weight gain) &prevent infection.
-*Chlortetracycline, for example is added by commercial fisherman to the ice in which ocean fish are packed in order to reduce decomposition.
Why antibiotic is add to our food
product?
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Milk is NOT allowed to be marketed if the milk gives +ve penicillin test, Why?
• Sensitization of consumers to penicillin
• The emergence of strains of pathogenic
microorganisms resistant to these drugs
• Allergic reactions in people sensitive to
penicillin might result from its ingestion
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Chemical tests for detection of antibiotics in milk
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1- a.Detection of penicillin in milkFew mgs + 2-3 drp sat. aq. phosphomolybdic acid
Intense blue color
Principle of reaction: color reaction involve 2 steps:-
a. Liberation of parent acid from its salt by phosphomolybdic acid b. Hydrolysis of the organic acid to give penicillamine which is a mercaptane (b, b-dimethyl cysteine) and is immediately oxidized to the corresponding disulfide by the phosphomoybdic acid with simultaneous formation of molybdenum blue.
Boiling water
close T.T With cotton
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Penicillamine (mercaptan)
Di sulfide + molybdenum metal ( blue)
Na
Oxid.
-Test is given by all pen. antibiotics
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Principle-:
this test depend on forming ferric hydroxamate whish is pinkish in color, it is formed when hydroxylamine reacts with the B-lactam group in the presence of fecl3
1)b. detection of Penicillin G in milk-:
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Principle of reaction:
NH2OH
Fe+3
ferric hydroxamate
Test is given by all -lactam -containig antibiotics (pen.,cephalosporine) & can be used for their detection.
NH
ONH
OFe+3
cont.
Na
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*test:- place apiece of filter paper on top of 250ml beaker
1-add 2 drops
1%KOH/ANHYDROUS MEOHIn center of the paper
follow immediatly
2-add 10 drops unhydrous
fecl3 /MEOH saturated with NH2OH.HCL
Sprinkle milk powder over wet area of the
paper
Procedure:
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1)b. Procedure for detection Penicillin G in milk-: cont.
Remove exss .
Observe the paper against
light
Pinkish spots
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2-Test for detection of doxycycline & oxytetracycline-:
*Sakagushi test-:-place drops of reagent in petri dish
Sprinkle milk powder on the surface of
reagent
Intense red (oxytetracycline)
Intense yellow (doxycycline)
Detection of some A.B in milk-:cont.
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-Sakagushi test differentiate between the different types of tetracyclines.
-it is give –ve result with penicillin &chloramphinicol.
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Detection of some A.B in milk-:cont.
3 -Detection of chloramphenicol-:
*Principle-:
Chloramphenicol is only known A.B which has character of an aromatic nitro compound. The NO2 group can readily be reduced to NO (nitroso) group by warming an aqeous sol. Of chloramphenicol with calcium chloride and zinc. The nitroso compound thus produced condenses, in acetic acid solution, with alfa-naphthylamine to yield aviolet azo dye.
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Principle of reaction:
CaCl2Zn
ON }
The NO2 group can be reduced to the NO (nitroso) group by warming
with CaCl2 and Zn
The nitroso compound produced condenses to yield a violet azo dye
N ]AzodyeNC10H17
naphthyl amine/acetic acid
Boiling water
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Milk(solid) contain chloramphenicol + 2 drops
10%cacl2
+several mg zn
dust
WB.
2min
2drops 5%sol.alfa-naphthylamine/ace
tic acid sol.
WB.
Intense violet color
3 -Procedure for detection
of chloramphenicol-: cont.
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Chromatographic detection of pen.G in milk-:
1-Thin layer chromatography (TLC):
Use: precoated silica plate
Solvent system: aceton-CHCL3-glacial acetic acid
(50:45:5)
RF =Distance traveled by the substance
Distance traveled by the solvent
Rate flow
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1cm
Drop of sample(Milk
sample+2drop of solvent )
Solvent front
Dist.travell by sub.
Dist. Travell by solv.
**Dry plate then spray it with potassium ferriccyanide followed by exposing the plate to iodine vapor
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RF value of penicillin G spot is consider as reference for comparison with the result obtained from our
sample .
RF pen. G =0.6-0.7
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• Preparation of Procaine Penicillin G• Detection of procaine penicillin G• Calculation of % yield • Determination of physical constants• Calculation of I.U of Penicillin G Na.
Part II
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Procaine penicillin, is a combination of benzylpenicillin with the local anesthetic agent procaine.
Following deep intramuscular injection, it is slowly absorbed into the circulation and hydrolysed to benzylpenicillin .
It is used where prolonged low concentrations of benzylpenicillin are required.
Preparation of procaine penicillin G
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Preparation of procaine penicillin G
- It is not effective orally.
- Different prepration to give different dose
-Procaine penicillin G is prepared by mixing equimolar of pen. G salt (Na or K ) and procaine HCL.
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Na
Penicillin G sodium
Procaine penicillin G
Procaine HCl
Preparation of Procaine penicillin
Penc.G + Procain HCl ProcainePen. G + NaCl
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Preparation of procaine penicillin G
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- Test for detection of procaine penicillin G:
*Phosphomolybdic acid test-:
-give +ve result with all penicillin
Procaine pen. G +2-3 drops saturated aqueous phosphomolybdic acid sol .
Cotton pecie
WB
Intense blue color
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**Color reaction involve two steps-:
First:- libration of parent acid from its salt by phosphomolybdic acid.
Second:- hydrolysis of acid to give penicillamine which meroptane=
B,B dimethyl cysteine oxidation of penicillamine to give the disulfide by phosphomolybdic acid to give disulfid with MO metal (blue in same time )
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1) Solubility2) PH3) Melting point
Physical constants of Procaine Penicillin G
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Physical constants of Procaine Penicillin G
1) Solubility
Pulverize the dry procaine penicillin you prepared
Weigh out 100mg and determine how much water is
required to dissolve this quantity.
Add 10ml of water to the salt in 50ml conical flask
and shake vigorously for several minutes.
If the salt has not dissolved, add 5ml more of water
and repeat
Continue the process, and record your results
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Physical constants of Procaine Penicillin G
2) PHMeasure the PH of the previously prepared solution of
procaine penicillin using a universal PH paper or a PH meter.
3) Melting pointUsing the melting point
instrument
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International unit of penicillin salt-:
1mg pencillin G Na =1667 I.U.
The international unit of penicillin is the specific penicillin activity contained in 0.6 mg of the crystalline sod. Salt of Penicillin G
The strength and dosage of penicillin are measured in terms of international units.
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- How many I.U in 1mg of procaine pen.G?
(procaine pen.G MWT=588.7 , PEN.G Na MWT =356.4)
1mmolProcaine pen. G 1mmol Pen.G Na
588.7 356.4
1mg X
X= 1 x 356.4/588.7 =0.605 mg
1mg pen.G 1667 I.U .
0.605 mg X
x= 1008.535 I.U.
Exampels 1
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-How many I.U in 1mg of benzathine penicillin? (benzathine pen. MWT=909.1 , PEN.G Na MWT =356.4)
( N.B:- 2mmol pen.G Na =1mmol benzathine penicillin)
Exampels2
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King Saud UniversityPharmacy Collage Pharmacognosy Depaartment
LAB#2
Glucose urine analysis & interaction with A.B.
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*Urinary glucose detection is of great importance for monitoring the condition of diabetic patients. The detection is done by the following reagents:
1 -Benedict,s reagent:- The reagent has clear deep blue color
- It contains acomplex of cupric citrate, when mixed with an equal volume of urine then warmed gently glucose will reduct to formed ppt.of cuprus oxide (CUO2)which is
bright red
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If glucose more or equal 0.15% +ve result
( bright red color )
Cupric citrate Cuprous oxide (CuO2)
Red.
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2 -Clinitest reagent:-The reagent comes as tablets, so we should prepare fresh solution for urine test.
-It contain copper acetat, so the reaction with the urine glucose with same principle (between cupric ion & glucose in urine ) bright red
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Advantage of Clini test over Benedict,s reagent-:
1-Clinitest provide fresh prepared solution of cupric acetat that superior sensitivity to Benedict,s solution which tend to give in accurat result when stored for extended period of time.
Advantage:
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Interaction of A.B:
* Some anti-infective agent give false-positive test
for glucose when use any reagent for testing
e.g,(tetracyclines, chloramphenicol,streptomycin,
P.amino salicylic acid, ascorbic acid in high dose
>( 1.5G/day) )
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Comment-:
1 -tetracyclines, chloramphenicol,streptomycin give
false-positive result with glucose in urine due to
their reducing property.
2-all cephalosporins interfer by different mechanism
a-react with CU ion produce insoluble brownish
black ppt. of cupric sulfat(CUS), these ppt. will
mask +ve color of test.
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Comment-:
b-Moxalactam is acephalosporin A.B that lacks sulfer in its ring structure, so it does not cause this interference
cont.
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-1ml of benedic,s sol or-1mg of clini powder+1pelet of NaOH
+1ml of sample
WBObserve color
How the test is do it?
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1( Sugar free urine
2( Sugar containing urine
3( Solution of a tetracycline
4( Solution of Cephalexin
5( Solution of Streptomycin
1( Sugar free urine
2( Sugar containing urine
3( Solution of a tetracycline
4( Solution of Cephalexin
5( Solution of Streptomycin
Sample
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Benedict,sCilin test
Sugar+urineRed or orange
orange
Urine+ceph.brownbrown
Urine+strep.blueblue
Urine+TCNbrowngreen
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To overcome this a disadvantage of previous reagent, lilly company has special tape which is selective
only for the dextrose :it is test-tape
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Glucose enzymatic test strip=test-tape method-:
This reagent is in form of paper strips
impregnated with :
*2 oxidizing enzyme: (glucose oxidase,peroxidase)+
oxidizable substrate(o-tolidine)+
yellow dye.
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How test-tape is work?
when paper impregnated into urine contain glucose
Glucose gluconic acid +H2O2
H2O2 +o-tolidine blue color
With the addition of a yellow dye ,The color range fromYellowlight greendeep blue
O2 from air
Glucose oxidase
peroxidase
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**compare color result with the chart and determine the % of glucose in
urine.
**yellow color mean NO glucose in urine
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* Non of the anti-infective agents interfere with the results of tes-tape
method
* Tes-tape method should be the method of choice for urinary glucose detection if the patient is receiving
any of these agents