phenofab by lemnatec and keygene
DESCRIPTION
Plant Phenotyping Services by PhenoFabTRANSCRIPT
PhenoFabEurope
For some details you need sharper eyes –
we‘ve got them!
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rbicide To
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(a)b
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Growth characteristics Root development
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isease
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Pheno
Fab
We make you know.
In the plant breeding industry phenotyping is one of the most crucial aspects of variety
development. Phenotyping is laborious and requires significant experience and a profes-
sional breeder’s eye. The high complexity of agronomically important traits makes it even
more difficult to perform objective and robust phenotyping in a high throughput manner.
In contrast, the enormous accomplishments that have been achieved in high-
throughput, sequence-based plant genotyping emphasize the need to scale up the
development of automated phenotyping technology. PhenoFab™ is a greenhouse
service operation that combines high-throughput, non-invasive technology with trait
interpretation to exploit phenotypic variation. It closes the “phenotyping gap”.
(a)b
iotic
str
ess
anal
ysis
Cold
Toler
ance
Growth characteristics Root development
Herit
abilit
y ca
lculat
ions D
isease
screening Salt Tolerance
Ph
enoFab
We make you know.
The PhenoFab™ services are delivered with a robust phenotyping platform in a greenhouse setup. Determination of the phenotype is
based on moving pots or trays with plants through a greenhouse compartment and fixed scanning areas containing image technology
to capture digital data. The plants grow in individual containers/tray-wells and are scanned at preset time points from various angles
using VIS (visible light), NIR and fluorescent imaging technology.
The facility has climate-controlled compartments that allow plants to grow up to 2.6 m under different environmental conditions, en-
abling experiments with different growth or stress factors. Depending on the plant size and experimental setup the capacity will vary
around an average of 1,100 plants.
Trait Spotting – our Business
The Fab – our Facility
The facility for the PhenoFab™ service provides:
n More than 300 m2 modern greenhouse space (PK2 certified) allocated for phenotyping
n Objective phenotyping studies in controlled environments, using
a dedicated greenhouse with specific hardware
n Digital phenotyping combined with specifically designed statistical approaches
developed for different type of Crops
n Trait analysis on e. g. colour, shoot & biomass development, heritability,
root development, canopy structure, as well as (a)biotic stress analysis
n Multilevel digital trait observations of individual plants
(e. g. angles, time series, light filtering)
n Proprietary software tools for image-to-digital phenotype and statistical analysis
n Completely integrated image and result database to store experimental
data (similar to LIMS)
n Water/nutrient supply controlled at individual plant level
Digital trait analysis is based on visible light and NIR (near infrared spectrum) imaging
as well as fluorescent imaging. RGB images are analysed using pixel values to identify
shape, colour and other morphological digital phenotypes. The NIR-infrared images are
specifically employed to reveal internal structures of plants, water content or other
(e. g. chemical) compositions. Lastly, the fluorescence imaging allows GFP-protein and
chlorophyll analysis to be performed.
Combinations of all imaging technologies can be used to resolve objects that have low
variability in any one spectrum.
The Service – Specifications
The Technology – a wide Spectrum
Scientific image processing since 1998It’s a Green Gene Revolution
KeyGene delivers sustainable molecular genetic responses to the world’s
need for stability in the yield, quality and health of crops. It’s our pas-
sion to explore and exploit genetic variation in vegetables and other
6F crops (Food, Feed, Fiber, Fuel, Flowers and Fun crops). For more than
20 years, we assist breeding companies by providing cutting edge mo-
lecular breeding technology and trait improvement platforms for their
own crop development. Therefore KeyGene has invested strongly in next
generation high-throughput Phenotyping technology to accelerate new
applications for breeding. Using this advanced digital technology makes
it possible to accelerate linkage of complex traits to the genetic variation.
KeyGene has its headquarters in Wageningen, the Netherlands, a subsi-
diary in Rockville, Maryland, USA and a Joint Lab at the Shanghai Institute
of Biological Sciences in Shanghai, China.
Contact: [email protected] · Phone: +31 317 466 866
Founded in May 1998 in Aachen (Germany) with 8 partners coming from
different backgrounds LemnaTec’s philosophy has always been to consoli-
date various competences. The interdisciplinary LemnaTec team combines
biologists, physicists, chemists, bioinformaticans, ecotoxicologists as well
as hard- and software engineers.
The LemnaTec team of scientists develops hard- and software solutions for
plant phenomics, high-throughput and high-content screening of plants,
seedlings, insects and other organisms and for the automated evaluation
of bio tests in ecotoxicology. Digital images are primarily taken by the
scanalyzer3D and scanalyzerHTS systems all set up in a modular design.
Using advanced LemnaTec image processing algorithms and fully inte-
grated data analysis, every visible parameter (e. g. colour, shape, size,
and architecture) is subsequently measured and further processed to be
correlated with experimental records (e. g. genetic data). Our aim: to
visualise and analyse the biology beyond human vision.
Contact: [email protected] · Phone: +49 2405 412 600
Dirk VandenhirtzCEO LemnaTec GmbH
Arjen J. van Tunen CEO Keygene N.V.
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For some details you need sharper eyes –
We do the PhenoFab
www.phenofab.comPhenoFab™ Europe
Agro Business Park 90
6708 PW Wageningen
The Netherlands
Phone: +31 317 466 866
Fax: +31 317 424 939
Email: [email protected]
Web: www.phenofab.com
Contact us!