Phage hunters 2014

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<p>Phage Hunters 2014</p> <p>Phage Hunters 2014Aida VargasDanilo Trinidad Prez RiveraAnthony Maldonado ColnHernn Mndez</p> <p> ProgramDepartment of BiologyProf. Eneida Daz PrezProf. Michael RubinObjectivesPresent the bacteriophages of interest.Establish adequate hunting strategies.Explain the processes carried out for preparing the phages after localization.Demonstrate growth acquired from the project so far.What is a phage?Bacteriophages, informally known as phages, are viruses capable of infecting a bacterium and replicating inside of it.These are typically composed of proteins which encapsulate their DNA or RNA genome.</p> <p>www.theepochtimes.comwww.shapeways.comWhy search for phages?Due to their specific nature, many phages can shed light not only on viruses, but also on their host.Moreover, identification of wild species can reveal how the given type of phage is evolving.Understanding these two things is transcendental to medical applications.Mycobacteria &amp; Bacilli </p> <p> smegmatisB. brevisExperimental DesignQuestionsCan new phages be found in the environment in Puerto Rico?What environments should be inspected to obtain a phage?HypothesesNew phages are widespread throught most ecosystems, therefore, there should be phages in Puerto Rico.The probability of finding a phage in a given environment depends on if it is adecuate for the phages host.Sample Collection</p> <p>Phage IsolationResultsSampleCoordinatesDescription PlaceDateAMC - #118.2 12 40 N; 66.1 W Slightly moist26C; 2cmCaguas, PRFebruary 2, 2014AMC - #218.212 67 N; 66.0 0 41.46 WLow moisture26C; 1cmCaguas, PRFebruary 17, 2014AMC - #318.2 12 9 N; 66 1 11.9 WModerate moisture26C; 3cmCaguas, PRFebruary 23, 2014AMC - #418.2 12 1.35 N; 66 2 13.7 WWet 26C; 3cmCaguas, PRMarch 2, 2014AMC - #518.2 12 58 N; 66 1 9.8 WVery moist 26C; 3 cmCaguas, PRMarch 9, 2014ResultsSampleCoordinatesDescription LocationDateAMC - 6#18 7' 2.553" N;66 6' 58.72" WMoist24C; 1cmCayey, PRFebruary 4, 2014AMC - #718 7' 7.172" N;66 6' 58.33" WDry26C, 2cmCayey, PR</p> <p>February 18, 2014AMC - #818 7' 6.686" N;66 9' 46.61" WMoist26C, 1cmCayey, PR</p> <p>February 24, 2014AMC - #918 7 8 N; 66 9 45 WMoist27.2CCayey, PR</p> <p>March 6, 2014</p> <p>YalinaResultsSampleCoordinatesEnvironmental descriptionLocationDateDTP - #118 0446.08N; 66 2147.29WDry; Superficial (1 cm); 20.6CCoamo, PRFeb/06/145:45 AMDTP - #218 0211.57N; 66 2222.25WMoist; Superficial (1 cm); 21.7CLos Baos de CoamoFeb/19/146:36 AMDTP - #318 0712.19N; 66 0926.60WMud-like; Superficial (3 cm); 21.7CCayey, PRMar/06/149:52 AMResultsSampleCoordinatesDescriptionLocationDateDTP - #418 0712.75N; 66 0926.89WMoist soil; Superficial (1 cm); 17.8CCayey, PRMar/18/149:13 AMDTP - #518 07'5.430" N;66 09'42.58" WMoist soil; Superficial (1 cm); 17.8CCayey, PRMar/18/149:24 AMDTP - #618 0712.19N; 66 0926.60WMud-like consistency; Superficial (3 cm); 18.3CCayey, PRMar/18/149:43 AMResultsSampleCoordinatesDescriptionLocationDate:TimeAV - #118427 N; 66.1156 WMoist; Superficial sample (1cm); 24CCayey, PRFeb/2/143:56PMAV - #218284 N; 661952 WVery moist, sample of dirt underneath clover leaves (1cm); 30C Dorado, PRMar/2/143:05 PMAV - #318730 N; 661548 WNormal moisture (underneath grass), 1cm;23CAibonito, PRMar/9/1411:07 AMAV - #418714N;661622WDry (underneath dried leaves; chicken nesting area), 1cm; 23CAibonito, PRMar/9/1411:36 AMResultsSampleCoordinatesDescriptionLocationDate:TimeAV - #518419N;661220WSome moisture, 1cm; 27CCayey, PRMar/16/144:19PMAV - #6 18429N;661156WMoist (underneath grass and dried feces), (1cm) 27CCayey, PRMar/16/144:45PMAV - #718 431N;661151WWet (mud) Superficial (1cm); 27CCayey, PRMar/16/146:24PM</p> <p>CacimarAiditaJR</p> <p>So, does Puerto Rico have phages?</p> <p>Where to hunt?</p> <p>X</p> <p>SummaryUnderstanding and characterizing bacteriophages provides plenty of useful information.</p> <p>Puerto Rico has an ample amount of uncharacterized phages in its soil.</p> <p>One should search for phages where their host could possibly found.Future WorkDilute obtained and purified filtrate to be able to obtain a web-pattern and high-titer phage filtrate.Characterize the capsid of the obtained phages through proteomics.Describe the structure of these phages utilizing Electron Microscopy.Sequence and annotate the genetic material of these phages.Lessons learnedLosing is winning.</p> <p>Practice makes perfect.</p> <p>Friends make it a lot easier.</p> <p>Patience is a virtue.</p> <p>Quitters never win. Special ThanksThe RISE program for opening the door for us to such a great opportunity.Dr. Rubin for volunteering to give us such a full and enriched experience.Nicolle Rosa for her help as our assigned TA.Gustavo and Giovanny for always being there to help.The cafeteria for the free sample collectors.Our moms for the Ziplock bags.</p> <p>Phage Hunters 2014Aida VargasDanilo Trinidad Prez RiveraTA: Nicolle RosaDr. Michel Rubin</p> <p></p>