, Christopher NlUes .' ... '" ':S;~t;f';;j .. ·· Personal Identification Sy§*em$'c'R~search Oirect~'ri ........ Human Factors/BehavioralSciencqDivision' ...... .

, c '. • • ? ' _ '. • , "'. .' ~',

: .. ,' ".

~D\\;~I); iHi· ".> .' ..•.•.. '.' .. ' '~;" .... ' ... Rapid @Qd low:co.~t; DNA-based verifi~tiQJl

of famU~Jelatiqn~hips (kinship )and)tV~lql1 "Jist ch~C~s of tnq$.~seeking asylum or ' . ... immigrationintotne United ~tates; ct}·ildren 'put upf~r overseas adoptions; and rri~~~-

,.:~asualtY,~jdentifi~tion$ . "'}.'

~1i~ach~J!'" .,0;QEI~ ne~ds aod. r~quiremE;)nt$ study. . ..,,\<<x.;, . ;.'I;{ ..•. , .••.. ' ......••

• R&D to automate and int~grat~.Q~~~; laboratp;J)':~~p~g~,~~'iO\e;;;.~~t~P§;, . . 'cr'!;/;;;;",;.;·· .. ... Use of DHS~mallbusin~~sinn9v~tive researGb::;'~Ii3JR1;prQ' " ;

ColiaborativeR&O progralJ'with,PQD and DOS"':"', . • Additional R&D toextend~il'l$t'iR:'anaJysi$capabilitie§!. , ....":.', .....

.... ..• NIST standardsdeveI9pm~nf~ndevaJ~n Ptl?rotQtY~pr~~~Cts . . ..... : User and public acceptanCei1i\ssi.... ." ...../ ]i~~~~';'.'i ·'·,c·::2~~ ... ;. 'i',':'Payoff"; ·'''''''·'';!·''4.:;i\l\\~;);;.,,22~

, • DNA screE;)ning for kinship i$ " . $500 to $100 per sample,

.;~~i;~~15"a~kid·Qj~AaqSed Screen i ns .. <" ;1,c'W,hy:Does DHS';oNeedRap,id"",DNA? ." ", '

·~eea'~.and.·~~~~ Uire'm~njt~' Fin401~9;;"'Y"":",:,,:,,,"""'~: "~~~~~. '<'){!i:,;~" ,,".. . .. . ... ::,. "'-, ;'; .. :'.' ,.:::, ',' ." " <

~il'\"

... ~ .. I)' tI I'f' f -, , ,~.. f I A .1Y III Ie ,1 e u USUS YIif/IJ '

~;,:.",-,;, '. ~,:;",,,:,.~:,~,:.\ -< ..... " ,: ... ,

t~~Ba'pcid ... PJ:.JA eqseg 'Scre~n i ngc~> ;~{iLll:iL."~;~1,. .~!~c,ppes DH~.~~~d~R~~!g;-DN~~ . ;'J ••

~~~~i\><"':t~',eed§;;:and. ,Re.q u i reme nt§., fJn~qirlg§: ~<.,.~:,<" ,', ' -" "', :,','" .:~.'::::. .':.' ';'. . , ',"",,'.. '::"!"~ ': .... : ... ';.. ," >:., ';"

,.~\I

~·A , , ." 1.;'''! A D,lr ill the Life of USCIS

, "'! , filii'"

• DOJ mandate to C(.)lIectDNA from . <d'i7~j~~;Q@I;lI(,m$ ',;,,,,,.., .•

. • PHS also Neegs DNA to'" ··"JiO.d Missiil~F"£d:?~,,, Persons Identification

\~ ~i>~:?c;T' "~c':g~rliBb'NA.:.a~sed~gr~~n i ng l~i:,"'~~S S~J~.l~apid DN~i~ased ~JQm.~t(iG.s Award~

~W1:~,.\DemqJ\t~trate~n automated des~tpp prot9t~~~ '., ..devic~i,~ibat verifi~s identity or kin~blp within" ' . . ~~ .. an hQui'fromONA samples '," ~~roadi .... : ''2hasetThree, 6 month, $100K stuqi~s

. ';. DHS needs and requirernents ~.~.seSSf:nent . ..... Performance metricsestabUsbed .. ' '. .... •.......... .., - Risk' 'assessment &··t~Chnic~r@r9hitecture·g.~~~lop.d

Phase .11: TWQ, 2 yeClr,$7S0Kllfototype de'l~I.QPme.nl.~ffQrt§ .... ; ............ . - Develop~ test, ~nd deUyer.Rf9totype RNA-bas'ed,gigrrietrip:ge"i,ca::: ' .. ,'.4,>,:.:.(;::1;;0:<.'

- Substantiate cost, speed,~ndperforma~ceJ~gain$ttbePhaseJrnetfi~t,l <,,: .;~. Phase III: Fully commerciatd .... ·>forO ........ ,other apRli.~~ti~o~:M;"\.~.'

. -Mas.~ .. g~sqalty situations, . JS foflowingmi:iii.:::.Z".;;c.w'¥!': evaqu~tions, igentifjqatiQn. . ............ ,' proces~ingof crirt1e~ . scene and suspectDNA . . ..... ·· .. f)ducatiQnaluse.s . ' ..

',"'" ,'.

",~,':' -'J:'~! ~,<{:: ::~,':~:~~~, >:' /~~; >"".

: 6 rll0nth, $10QK • Develop cjetaUf3d use c~ses

Jdentif¥key teqbnical risks and \

Phase oals: 2year,$75QK . . . " .'. ..'... ...... , .. '......... .'.'0." .. . '. D~velop a prototypesystf3rn •.. y~ipg a dispos.~bl~miQrQfluigig"Qa·r1(i,g,g§'("J~:;:;';'~\;;'·', •.

containing all of the reqLJjred"f~agents and mat~[ials ..••.....•. . >~· ••..••.•••..• ·.<.L::·;.:}>4~~.,.l, .•. ~ii.(.'.:N.i .• •••.••...•.

• Employsalow-costdiSPQ§~ble mi .... '. qhipen~Dlingpr~ci~e~m~ni.p:ijlitiQ\h.~ of liquid drQplets using an array . ,.'. ··ectrQd~§formedQll.;Eloin~~Rij'r~iv~i· .. print~d~cirquit-board (PCB) , .' .... , "'~ '"~"\,,,,i{L,,:.~1f~:t~\:~~~~;\~Y,,;J.~~L;i:'"

,'~.'.' .. '''-. ~'~U)id,;pNA Prpjects ······j'Qig~tal rV1;icrofluid,l9~": '.'

' .... ;~. . . ); .<~.;:~, ':.. . :.... , '. .:., \: .. '. .

· .•.•...• 'c .i~~pid;~PNAProj~gt~ .' ........ ' "BiQCh'ip" ' . . .

Qal~~;i:fmo. $100K :: DHSJleeds and requirements

, , :~ ':: '. '; -;. , ' . . . .~,~ ' .. ' ,

~'i'surve~< .', '.. ..... ..... '" ?"Sstaijt~h pE:)qormancE:) met;ies • '§tudy:'<'andiaate technologies.1~~-·~r2 (~1r.isks'.' .. .. Define a viable architecture .

:.>;\\,"

'>:~'Phase 11,2 years, $7~OK • R&D to expand kinsgip,

analysis beyond parent/chil" relationships to siblings grandpClrents/ grandchi

.. )'\4R~~.i~~·dNA· ~.~.se·(f·.S:q£Q~o.ing· "J.\g~~l~rated NucJ~a~.DNJ.\··§g4IR~1~pr'····

·;·~ded.d~D, DOJ, DH,§Rroj~()t ,', ·.con~~ct 18f'T)onth R&D eff9rttod~\(~I() 'Rr9tQt¥p,ei~2

';~";';In itial:~eskt8R-§i~e, aut9mat~d 'S¥Sf~,Qi·W!f~19.W1¥~.I.Mm~£jLl~~~~·" 'upit cQ~~t of$,75K availaple 6 mqn~h§att~r.'S}Qrqtq~ype;::J:'!::'::?:"~0: .·aUrreH\ manual proQeS§ingstep§ al,lt~tniMeaari([ ,.,\ .. ~:;;, t. .~.

integrated into ade§ ", ··..~i?e devjc~.i . "Y';"C~;;;<";;;;i

secure data processing. data analysis. & comm

. "':"'<:'" ,~;:~< :;: )r~'iL;·· < ," .. .,">«.'"' j;>.r~ .. ".. .. >~ .. " ': ;'. ,,'

1rRa6t~:DNA.· .~a§ed·· .. ·§cr~eni.ng J~nt R~pid-DtiJ.\ ArJa,ly~i~,PtoJ~qt

'~SV~tiona~;~igUire~~t; . Sam~le Ab!,I;t~iS ~\'l'~\;~()n~techni~l~iir with 1tl~Of training 1. Sample-in\tQ:profU~outin T::;~1 '2~:Pr~fil~,generatio~,from bUccalswab h, G=45 m ,,' ", ",' ,

',"" p()m~nual user:iPan;pulati,onsrequired

3.$'t~Ptlytwo Pt~on lift in,,$l~ min & ruggedi~ed to t;' ;' ortatio,,'vibrations , .... \\... . ":," .";."; ": ..

4. SetacifYr.liabilitv~'f. systemJ<MTIF 5. Ro~tin~maint~~:~~intenlals a= 1

month;;;" . "S' ' • ./~~\ :

' •. ~.,~o routine alignment or recalibration ':i.,)'Qperate using 120\1, drawing < 1:5 'Ltmps '

",'et,gverall dimensionss 6 cu.ft., oolto ixceed 30 in., weight < 50 kg :;)';.' . . ".

9~;;;$.vstemcost $$275K, ;~stlsample s $100

~;';1, Q.Cc;msumables must, be disposable, <;~~;>pre .. loaded with reC;lgents and sealed

i£Prototypes at TRl6 at end of 18 mo .. : "k~.·. ~.'., >":,:

2. SirnuJtaneou,pro~ssing ofT:;;ft samples, G =1,.16 samples' .

3. Rea~entstabili~ oft=3 m~~At ." 20-30oC. G=6 mQ.At -1Q..50oC

'0. \" ,", ':.".,. :' .... "' .. ,,', '-

~. " DNA extraction &pu~ficatJQn . compara9,e te) bena~tQP .' .. methods"':: . . ' .. ; ..... ':. ", .. ," "

5.PGR"rnpl'fication of ~ 16,.tQ9t . :...(GPRI.~) inS 30 min, G= '·:<.\.\c./

Additiogat reagent sets . .>; •• \ .,'$" , '. '. , • ~~~ '. : • ' . ." . ." .' "

",'.,: "'0 ". :,'

r;,;".,"'· ___ ~~~~~.~~:~! · .. that ••• rtli~··p()sili~~~ &r_:~~i,~;f\.:;s.\ief"~··i:.·. . QnPQard'cgmpJ.l ., ..... .;.. ..' . ,

"'S'.:.'<'J.~~:.; ... ' '~;<:.'" ":", , ".:.". . .. ~;~:.,; ,;.\2 . .J .,.,' :l:.\".:·:~. ;~:·\yJ~~~'t;:{:}';'::~;:\{f:.~<:·>, !~~;::~.(~\~:< .. ' .;' ..

6. So1\WC;lr,,:.'M\IiodoY/sXp,:;netwmt.:2'·· connectiot\·,~.... . 't¥?

.~TRaliele calling::p •.. generation, Comms? Sy.st~mgontrol

7~>'Wirelf;lss;.wj(ed, . & USB, natwolrk

",: ' -_,~'2'

• Stq;rndardsJqr Rel.ationship·Testlng·· LabQratori.e.s, 9th edition . .. '.

t Rei~jrem~nts that mu~tbeimpl~l11~p!~S! by laboratories ClccreditedbyM§J3tQ[.

h;/relationship testi.ng ·!').·;.x.'·':'· Y .....

• .. Includes data CQ.U~ct~g;>"~~ith the Simp(~,;~5;,; hoW long sampl~ssh9\:i1~ be. retained,fgf: how many test ~art1p/~S Jd .... .. n'tf6..;, ..... . valid.ate device perfo.. .thaf':::·;,;·::;+t should be maintaineQ,' , ... ' ··"«1.\';'~·;;;;::·~~~,';{Ji~~~i~8t[:':i~:C.~;~;,~~;

test reports,etc.

~::Rapi~;C'NA .Data'i ... SbariO.g'Standards ," ',". • ,·v. ," :", .. - . . . '.... . . .. : .. " ," .," • ;;:": :.:.; .... ,. . ,"I .,

'<::. ;.~}~ .. :.?,':'"''

tliST Special Publication 500-271 ,:;&.c~N<··:···<;~:'<·~' ':

hang~:QUingerprint info~~ati9.Q~~~., . .,~,'.~;t,t.!; E:::;:n::::::::, • pd,"~,;.!l, ed i ,,<200 7a,nd~O, "OS",/ ',?, •• ".·?·,~:.,.)i;;" J,i;.: LY' Facial, & Other Biometric Information - Part 1

,,"~ . , )-:/ :~'<"':.L·?i';.· ..

~ Irii~exCh~l1:ge' ," ", ,\;"),<i,,,;:

",- Conformance toPm~.~~np~rds :,~'~1".; ~Updated faci,alC:l9(fJin~~rRrint specifl~~li~m~,

,. I, . , .•

~~\ ., , ,,',' A )~>,,' ,-,.'t> ~J:"'" ,

...

Ntsr

nY9,QY and Civil Right§l~ib~rties 1',FBI~'s BEl:'"c}~Yigil .. nt: ·h'>' ...••• / ",

., CQO.IS locj'are chosen tQ.Q.Qt re\(~~1 phx~lcal traits, race,', eth~icity, d.i~easesusceptibUity

, or.otper sensitive·infqrmati,pn ' .. ' .' Jud~,~,aryoversight cornrnittees' ~~~----~........,..,...~~~---­,<,advised 180day~t;>eforeany

<.' change to core' g~net'G,J1Jarke(§,," • No instance of rn.~iSUSEt'Jn· .. < .' ............ ~." ••• ~::<, .... ~~~~

~ 40 year hlstorY~i19f P.£Q9fCim • . Congress, ACLLJlg~tQlnstilut.e

and' others remain ·sensif .... " u.<~:; .

/ Privacy Act Qf19Z4

o