hnolo

the bands in the SSCP gels, 37 clones of eubacterium and 17 clonesof archaebacteria were obtained (Diaz et al., 2006). The 37 clonesof eubacterium were classified into the following six phyla: Pro-teobacteria, Chloroflexi, Firmicutes, Bacteroidetes, Actinobacteriaand Thermotogae with a percentage of 48.66%, 16.22%, 13.60%,10.91%, 8.11% and 2.70% respectively. About 95% of the 17 clones ofarchaebacteria were affiliated with Methanogenium while 4% wasunclassified in the GeneBank.

S636 Abstracts / Journal of Biotec

Liu, H., Gort, S., Logan, B.E., 2005. Electrochemically assisted microbial productionof hydrogen from acetate. Environ. Sci. Technol. 39, 4317–4320.

Liu, W.Z., Wang, A.J., Ren, N.Q., Zhao, X.Y., Liu, L.H., Yu, Z.G., Lee, D.J., 2008. Elec-trochemically assisted biohydrogen production from acetate. Energy Fuels 22,159–163.

doi:10.1016/j.jbiotec.2008.07.1472

VII3-Y-031

Algicidal effect of Pseudomonas fluorescens against Stephan-odiscus Hantzschii (Bacillariophyceae) during winter freshwaterblooms: Biological impact of algicidal activity in mesocosms

Jung Seung Won 1,∗, Lee Jin Hwan 2, Han Myung-Soo 1

1 Department of Life Science, Hanyang University, Seoul 133-791,Republic of Korea2 Department of Life Science, Sanmyung University, Seoul 110-743,Republic of Korea

E-mail address: diatoms@hanyang.ac.kr (J.S. Won).

Stephanodiscus hantzschii frequently evokes massive winter bloomsin freshwater. We studied response of an algicidal effect againstnatural S. hantzschii blooms and planktonic community to theinoculation of an algicidal bacterium, Pseudomonas fluorescensHYK0210-SK09 (SK09) (Jung et al., 2008) in mesocosm experi-ments. The inoculation density of SK09 (5 × 106 cells mL−1) in smalland large-scale mesocosms caused maximum 94.87% and 84.93%decrease of S. hantzschii cells, respectively. Therefore, the algicidalbacterium (SK09) was effective a bio-agent to control S. hantzschiiblooms in field application as well as indoor enclosure experiments(Jung et al., 2008). In terms of physico-chemical factors, results oftwo mesocosms studies were different. In small-scale mesocosm,the pH and dissolved oxygen concentrations were decreased, whiledissolved inorganic nitrogen, phosphate and silicate concentra-tions were significantly increased by activity of SK09. However, inlarge-scale mesocosm, the environmental factors were less variedthan those of small scale mesocosms. These variations of environ-mental factors between small- and large-scale mesocosms may beaffected by structure of each mesocosm setting such as enclosurescale, involvement of sediment and water exchange of a large-scale mesocosm. In planktonic communities, abundances of somephytoplankton species a little increased with rapidly decreased S.

hantzschii cells. Heterotrophic flagellate and ciliate communitieswere significantly increased after bacterial assemblages, probablyas a result of their consumption of increased SK09. Previous reports(Mayali and Azam, 2004) demonstrated that the bacterial bloommay be associated with an increase in algicidal bacteria, causinga secondary problem of the high abundances of algicidal bacteria.However, in this study, increasing bacteria were controlled by theirpredators such as heterotrophic protists. It is therefore possible tohave stable biological control without disturbing the natural systembecause of self-cleansing action of nature.

References

Jung, S.W., Kim, B.-H., Katano, T., Kong, D.-S., Han, M.-S., 2008. Pseudomonas fluo-rescens HYK0210-SK09 offers species-specific biological control of winter algalblooms caused by freshwater diatom Stephanodiscus hantzschii. J. Appl. Micro-biol. 105, 186–195.

Mayali, X., Azam, F., 2004. Algicidal bacteria in the sea and their impact on algalblooms. J. Eukaryot. Microbiol. 51, 139–144.

doi:10.1016/j.jbiotec.2008.07.1473

gy 136S (2008) S633–S646

VII3-P-001

Performance and microbial community analysis of an anaerobicbaffled reactor treating molasses wastewater

Sheng Chang 1,2,∗, Jianzheng Li 1,2, Liu Feng 1,2, Yan Zhao 1

1 State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology, Harbin 150090, China2 Department of Environmental Science & Engineering, Harbin Insti-tute of Technology, Harbin 150090, China

E-mail address: ljz6677@163.com (S. Chang).

A seven-compartment anaerobic baffled reactor (ABR) with aneffective volume of 64.4 L was introduced to treat molasses wastew-ater. The performance characteristics and microbial communitiesin the ABR were investigated within the 120-day operation period.A chemical oxygen demand (COD) removal of 93% was achievedin the ABR during the steady stage at the organic loading rate of3.2 kg (m3 d)−1 (Baloch et al., 2007). The alternation of microbialcommunities along the compartments were estimated by single-strand conformation polymorphism (SSCP) (Marion et al., 2004)targeting the V1–V3 region of 16S rDNA genes during the steadystate under the OLR of 3.2 kg (m3 d)−1. The results indicated thatthe population and the structure of microbial community in eachcompartment had no succession anymore, while a remarkable dif-ference was observed among the seven compartments. Accordingto cloning and sequencing results of the bands excised from SSCPgels and phylogenetic reconstruction, the acidogenic bacteria, suchas Escherichia sp., Anaerophaga sp., Corynebacterium sp., Anaerolineasp. and Clostridium sp., etc., dominated in the first three compart-ments, while methanogenic bacteria such as Methanobacterium,Methanogenium, Methanosarcina, Anaeroliea, etc., existed in the lastthree compartments. The largest number of population was in the4th compartment resulting from its function as a buffer zone. From

References

Baloch, M.I., Akunna, J.C., Collier, P.J., 2007. The performance of a phase separatedgranular bed bioreactor treating brewery wastewater. Bioresour. Technol. 98,1849–1855.

Diaz, E., Stams, A., Amils, R., Sanz, J.L., 2006. Phenotypic properties and micro-bial diversity of methanogenic granules from a full-scale UASB reactor treatingbrewery wastewater. Appl. Environ. Microbiol. 72, 4942–4949.

Marion, L., Jean, P.D., Jean, J.G., 2004. Diversity of the archaeal community in 44anaerobic digesters as determined by single strand conformation polymorphismanalysis and 16S rDNA sequencing. Environ. Microbiol. 6 (8), 809–819.

doi:10.1016/j.jbiotec.2008.07.1474