147

PERFORMA FOR THESIS

Data Collection

Particulars of patients:

Name…………………Age/Sex……………..Religion………………Occupation……

…

Religion…………………………

Address…………………………………

Presenting complaints:

Pain: Right knee/left knee/DIP / Wrist /Hip/Ankle

Body Height & Weight (in Kg) ……………………………………………………

Symptoms: Swelling/Warmth/Stiffness/Crepitus/Tenderness

Duration of Stiffness :> 30 min/<30 min/30 min

Severity: Mild/Moderate/Severe

Previous History

Illness: Yes/No Diagnosis ……………………

1 Case No. 5 Date of Admission

2 MRD No. 6 Date of Discharge

3 Hospital No. 7 Department

4 Bed No. 8 Unit (if any)

148

Accident that may account to trauma/infection/joint intervention/RA

Family history: Hereditary disorder: Yes/No

Social and Occupational history:

Nature of occupation…………………… Use of tobacco: Yes/No

Use of alcohol: Yes/No Diet: Rice/Chapatti

General appearance

Build: Slim/Average/Overweight/Obese

Body height …..ft…..inches Weight…..kg

Treatment history

Drugs : Yes/No Surgery: Yes/No

Oral contraceptives: Yes/No Arthroscopy: Yes/No

Others…….

Radiological impressions…………… Diagnosis……………………

149

CURRICULUM VITAE

1. NAME: Sonam Choden Bhutia

2. DATE OF BIRTH : 28th /02 /1980

3. EDUCATIONAL QUALIFICATION

Graduation Year

University : North Bengal University 2002

Specialization: B.Sc

Post Graduation Year

University : Sikkim Manipal University 2005

Specialization : Msc (medical biochemistry)

Doctor of Philosophy (PhD)

Institution : Sikkim Manipal Institute of Medical Sciences

Registration Date : 14th /8/2008

150

LIST OF PAPERS

PUBLISHED ON THE BASIS OF THIS THESIS

1. Sonam Choden Bhutia, Mingma Lhamu Sherpa, S.K.Dewan and T.A.Singh

(2015) (Accepted). Indian journal of Medical Research :IJMR_1235_14

2. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa (2014).

Production of polyclonal antibody against osteogenic protein-1 (OP-1): its role

as diagnostic tool in osteoarthritis. Singapore Medical Journal; 55(7):388-

391.

3. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa (2013)

Cartilage metabolic markers and antioxidant: its correlation in osteoarthritic

patients of Sikkim. Int J Cur Res Rev, 5(1): 58-63.

4. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa (2013)

Correlation of the Osteogenic Protein-1(OP-1) with age, cartilage metabolic

markers and antioxidants in the osteoarthritic patients of Sikkim. Journal of

Clinical and Diagnostic Research, 7(8): 1565-1567.

5. Sonam Choden Bhutia, T.A.Singh, Mingma Lhamu Sherpa. Diagnostic use of

polyclonal antibody against osteogenic protein-1(OP-1) in osteoarthritic

patients of Sikkim. ACBI Souvenir 40th

National conference of Association of

clinical biochemist of India (ACBICON), New Delhi. December, 2013.

151

PRESENTATIONS IN CONFERENCES

1. Diagnostic use of polyclonal antibody against osteogenic protein-

1(OP-1) in osteoarthritic patients of Sikkim. 40th

National conference

of Association of clinical biochemist of India (ACBICON), New Delhi.

December, 2013.

.

152

CURRICULUM VITAE

1. Name : Dr. T. Amumacha Singh

2. Date of birth : 1st /03/1953

3. Qualifications : i) CBSE : March 1968

ii) Bsc : April, 1971, Jabalpur University

iii) MBBS : June 1979, Guwhati University.

iv) MD : Ma/Ap 1992, Nagpur University

v) Post MD : Aug.1998, AIIMS, New Delhi.

vi) FIMSA : 1999, India

vii) FISBT : 2008, India

4 .Medical Registration no. : 8384, Assam Medical Council

5. Present Designation : Professor & Head, Department of Biochemistry &

Biotechnology, SMIMS.

6. Permanent address : Khurai Chinganbam Leikai, Tinsit Road, Imphal-

795010,Manipir, India

153

WORK EXPERIENCE

Professor SMIMS,Gangtok 1st/7/2006 Till date 08 years

Assoc.Professor -do- 13th/5/2002 31

st

/3/2006

4yrs,01 month

Asstt. Professor RIIMS, Imphal Feb.1994 April,2002 8 yrs,7months

Demonstrator -do- Jan,1981 Feb.1994 13 yrs,1 months

MD Guide RIIMS,Imphal &

SMIMS,Gangtok

1996 Till date 18 yrs

PhD Guide SMU,Gangtok 2008 Till date 6 yrs

PhD Examiner SMU,Gangtok 2004 Till date 10 yrs

Publications

1. T.A.Singh ; K Cheirmaraj; MV Reddy; R Narang; BC Harinath .Diagnostic use

of filarial antibody and antigen isolated from hydrocele fluid for human filariasis.

Indian journal of experimental biology. 1993, Volume: 31:1- 4.

2. M Maneesh, H Jayalekshmi, T Suma, S Chatterjee,

A Chakrabarti, and T A

Singh. Evidence for oxidative stress in osteoarthritis .Indian J Clin Biochem. Jan

2005; 20(1): 129–130.

3. Sonam Choden Bhutia, Mingma Lhamu Sherpa, S.K.Dewan and T.A.Singh

(2015) (Accepted). Indian journal of Medical Research :IJMR_1235_14

154

4. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa (2014).

Production of polyclonal antibody against osteogenic protein-1 (OP-1): its role as

diagnostic tool in osteoarthritis. Singapore Medical Journal; 55(7):388-391.

5. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa (2013) Cartilage

metabolic markers and antioxidant: its correlation in osteoarthritic patients of

Sikkim. Int J Cur Res Rev, 5(1): 58-63.

6. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa (2013)

Correlation of the Osteogenic Protein-1(OP-1) with age, cartilage metabolic

markers and antioxidants in the osteoarthritic patients of Sikkim. Journal of

Clinical and Diagnostic Research, 7(8): 1565-1567.

7. Dr Tshering Paljor , Prof T A Singh and Prof. Mingma Lhamu Sherpa

ACBICON Analysis of Gall stone among patienta attending State Referral

Hospital Sikkim. ACBI Souvenir.40th National conference of Association of

clinical biochemist of India (ACBICON), New Delhi. December, 2013.

8. Dr. Anne Deborah Rai, Prof T.A Singh and Prof Mingma Lhamu Sherpa.

Comparison of bone turnover marker in pre and post menopausal women with

osteopenia and osteoporosis in Sikkim. ACBI Souvenir 40th National

conference of Association of clinical biochemist of India (ACBICON), New

Delhi. December, 2013.

155

9. Sonam Choden Bhutia, T.A.Singh, Mingma Lhamu Sherpa. Diagnostic use of

polyclonal antibody against osteogenic protein-1(OP-1) in osteoarthritic patients

of Sikkim. ACBI Souvenir 40th

National conference of Association of clinical

biochemist of India (ACBICON), New Delhi. December, 2013.

10. B.Paul, T.A.Singh, P.K. Mitra.Effect of leaves of Cassia alata Linnaeus

(Sasesalpiniaceae) on the growth of Staphylococcus aureaus Rosenbach,Pleione

6(2):341-347.

11. B.Paul, T.A.Singh, P.K. Mitra.Effect of solvent,temperature and time on

extraction process of antimicrobial factor of Staphylococcus aureus present in

Cassia alat L,Bioscience Guardian,3(1),2103:206-211.

12. B.Paul. T.A.Singh, P.K. Mitra.Isolaltion and structural determination of an

antibacterial constituent from the leaves of Cassia alata Linn, Journal of

Pharnacology and Phytochemistry: 2(1),2013.

13. Sonam Choden Bhutia and T.A.Singh .Thyroid dysfunction in Sikkimese

populations-an acute health problem. ACBI SOUVENIR 35th

National

conference of Association of clinical biochemist of India (ACBICON).Kolkata.

December, 2008.

14. Dr.A Ghosh, Ms Y.Bhutia, Dr.T.A.Singh and Dr.M.L.Sherpa. Nitric oxide and

lipid profile in type II Diabetes mellitus-A case control study in East Sikkim,

Indian Journal of Clinical Biochemistry (suppl),2009/24:246.

156

CURRICULUM VITAE

Name : Dr. Mingma Lhamu Sherpa. MD, FIBST

Designation : Professor

Contact information : Department of Biochemistry, SMIMS, 5th

Mile,Tadong

Gangtok-737102, Sikkim, India

Email : mingmals@yahoo.com

Date of birth : 12th of December 1973.

Academic Background

DEGREE Year COLLEGE

MBBS 1998 Lady Hardinge Medical College, Delhi

M.D. (Biochemistry) 2004 Lady Hardinge Medical College, Delhi

WORK EXPERIENCE

JOB

DESCRIPTION

DURATION INSITUTE

Resident Intern

Jan 1998 to Dec 1998 Lady Hardinge Medical College,

Delhi

Junior Resident,

Department of Blood

Bank

Jan 1999 to Jun 1999 Lady Hardinge Medical College,

Delhi

Junior Resident, Jul1999 to Oct 1999 Lady Hardinge Medical College,

157

Department of Surgery Delhi

Junior Resident,

Biochemistry

July2001 to April 2004 Lady Hardinge Medical College,

Delhi

Assistant Professor,

Biochemistry

July 2004 till July 2009 Sikkim Manipal Institute of

Medical Sciences, Gangtok,

Sikkim, India

Associate Professor,

Biochemistry

July 2009 till July

2013

Sikkim Manipal Institute of

Medical Sciences, Gangtok,

Sikkim, India

Professor, Biochemisty May 2013 onwards Sikkim Manipal Institute of

Medical Sciences, Gangtok,

Sikkim, India

Awards and Honors:

1. Received the Mariamma Verghese Award, 1999 for the most compassionate house

surgeon at Lady Hardinge Medical College, New Delhi

2. Nominated for the Sita Devi Award for the Best Scientific paper 2006 at

ACBICON 2006 at Pune for the paper titled ‗Association of NO with c-FOS and c-

JUN in Breast Cancer‘

3. Fellow of International Society of Biotechnology, 2008

4. Received the Exemplary Performance Award- Programme Implementation

(Biochemistry) 2008 at the 8th Convocation, Sikkim Manipal University.

5. Fellow of Fogarty International Center, USA-2013-2014

MEMBERSHIP OF NATIONAL AND INTERNATIONAL BODIES:

1. Life Member of Association of Clinical Biochemists of India

2. Life Member of International Society of Biotechnology, India

158

3. Member of Institutional Ethics Committee, SMIMS, Gangtok, Sikkim, India 2012

onwards

4. Member of American Psychopathological Association, New York, 2013-2014

5. Reviewer for Addiction 2014

6. Reviewer for International Journal of Medicine and Public Health 2014

7. Unit Head , UNESCO Bioethics Chair, Sikkim, India

Training:

1. Organizing member of CME on ‗‘Molecular Medicine‘‘ in Lady Hardinge

Medical College, New Delhi,2003

2. Regional Workshop on ‗‘ Telemedicine & E-Healthcare Service Delivery‘‘,

Sikkim Manipal University.

3. Workshop on ‗‘Teaching learning‘‘ India, Sikkim Manipal University, 2006.

4. Member and Delegate of the ‗‘ Regional workshop on RNTCP-WHO‘‘,

October ,SMU,India,2006.

5. Workshop on ‗‘Scientific writing‘‘ SMIMS,Nov,2006

6. Participated in the CME ‗‘Postgraduate Curriculum in the

Millenium‘‘,SMU,Nov,2006

7. Workshop on ‗‘Laboratory Management‘‘ in AFMC Pune, Nov,2006.

8. Workshop on ‗‘Clinical skills and OSCE‘‘May 2008,Sikkim,India

9. Organizing member and Delegate in CME‘‘Awesome

Pulmonlogy‘‘May,2008

10. Delegate in update on‘‘Respiratory diseases in children‘‘, May 2008

159

11. Resource person,CME on Tuberculosis by State Task Force, Sikkim ,June

2008

12. Organising member in CEZIPS-East,Gangtok,2009.

13. Participated in the ‗‘Update on Respiratory diseases in children‘‘ held in

SMIMS,June 2011

14. A member of organizing committee for ‗‘24th annual conference if All India

Rhinology Society-Rhinocon-2011‘‘,SMU,April 2011

15.Attended the ‗‘ Hands on workshop on Mesenchymal stem cells held at Manipal

Institute of Regenerative medicine, Bangalore,July 4th -9

th, 2011.

16. The fogarty international centre INDO-US program in Non-communicable

Diseases (CNCD‖S ) Workshops on Research Skills building on 14th and 15

th of May

2013.

17. CME on stem cell , 20th June 2013 conducted by Dept of Biochemistry, SMIMS

18.Organizing member for the annual convocations for graduates of SMU.

19. Coordiantor of BScMLT course,SMU.

PUBLICATIONS

2002

1.Jain A,Mangal A,Sherpa M.L, Gayatri R and Goyal U .Placental ALP in serum and

placenta in pregnancy in Preganancy Induced Hypertension SOUVENIR,9th Asia

Pacific Congress of Clinical Biochemists and 28th annual conference of ACBI,2002.

160

2003

2.Battacharjee J and Sherpa M L.Role of Nitric oxide as endogenous scavenger of

lipid peroxidation trends in clinal Biochemistry and Laboratory medicine,2003.

3. Battacharjee J and Sherpa M L.Nitrix oxide and Transcription factor API in Breast

Cancer,ACBI Souvenir,29th annual conference of Association of clinical

Biochemistry of India,Jaipur,2003.

2006

4.Sherpa ML,Bhattacharjee J,Ravi B,Prusty BK and Das BC.Association o fNO with

c-FOS and c-JUN in breast cancer,ACBI Souvenir,33rd

annual conference of

Association of clinical biochemisty of India,Pune,2006.

2008

5.Mingma Lhamu Sherpa,Jayashree Bhattacharjee ,BINa Ravi,Bhudev C Das.NITRIC

OXIDE,MDA LEVEL SIN SERUM AND GPX ACTIVITY IN WHOLE BLOOD

BREAST CANCER PATIENTS.Indian Journal of Clinical Biochemistry;Dec

2008,vol.23(suppl).

2009

6.Mingma L Sherpa,‘‘Seasonal Affective Disorder‘‘,CEZIPS-East Zone

Souvenir,Gangtok,2009.

7. Dr.A Ghosh, Ms Y.Bhutia,Dr.T.A.Singh and Dr.M.L.Sherpa. Nitric oxide and lipid

profile in type II Diabetes mellitus-A case control study in East Sikkim,Indian Journal

of Clinical Biochemistry(suppl),2009/24:246.

2013

161

8. Dr Tshering Paljor, Prof T A Singh and Prof Mingma Lamu Sherpa ACBICON

Analysis of Gall stone among patienta attending State Referral Hospital Sikkim.

ACBI Souvenir. 40th National conference of Association of clinical biochemist of

India (ACBICON), New Delhi. December, 2013.

9. Dr. Anne Deborah Rai, Prof T.A Singh and Prof Mingma Lamu Sherpa.

Comparison of bone turnover marker in pre and post menopausal women with

osteopenia and osteoporosis in Sikkim. ACBI Souvenir 40th National conference of

Association of clinical biochemist of India (ACBICON), New Delhi. December,

2013.

10. Sonam Choden Bhutia, T.A.Singh, Mingma Lhamu Sherpa. Diagnostic use of

polyclonal antibody against osteogenic protein-1(OP-1) in osteoarthritic patients of

Sikkim. ACBI Souvenir 40th

National conference of Association of clinical biochemist

of India (ACBICON), New Delhi. December, 2013.

11. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa. Production of

polyclonal antibody against osteogenic protein-1 (OP-1): its role as diagnostic tool in

osteoarthritis. Singapore Medical Journal (accepted), SMJ-2012-623R1.

12. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa (2012) Cartilage

metabolic markers and antioxidant: its correlation in osteoarthritic patients of Sikkim.

Int J Cur Res Rev, 5(1): 58-63.

162

13. Sonam Choden Bhutia, T.A.Singh and Mingma Lhamu Sherpa (2013)

Correlation of the Osteogenic Protein-1(OP-1) with age, cartilage metabolic markers

and antioxidants in the osteoarthritic patients of Sikkim. Journal of Clinical and

Diagnostic Research, 7(8): 1565-1567.

2014

Bhutia SC, Singh TA, Sherpa ML. Production of a polyclonal antibody against

osteogenic protein-1, and its role in the diagnosis of osteoarthritis. Singapore Med J

2014; 55(7): 388-391.

Khandelwal B, Khatri D, Patnaik SK, Sherpa ML, Kar S. Microalbuminuria as an

overrated indicator of target organ damage in hypertension: a hospital based cross

sectional study. Int J Res Med Sci. 2014; 2(3): 881-885 doi: 10.5455/2320-

6012.ijrms20140822

Bhutia SC, Singh TA, Sherpa ML.Correlation of cartilage metabolic markers &

antioxidants with the severity of knee OA & its demographic characteristics.(

Accepted for publication by IJMR_1235_14).

PAPER/ POSTER PRESENTATION AT NATIONAL AND INTERNATIONAL

CONFERENCES

1. Placental ALP in Serum and Placenta in Pregnancy Induced Hypertension 9th Asia

Pacific Congress Clinical Biochemistry & 28th Annual Conference of Association of

Clinical Biochemist of India, 2002

2. Nitric Oxide and Transcription factor in AP1 of Breast Cancer 29TH Annual

Conference Of Association of Clinical Biochemist of India, Jaipur, 2003

3. Association of NO with c-FOS and c-JUN in Breast Cancer, Sita Devi Award

Session. 33RD Annual Conference of Association of Clinical Biochemist Of India,

Pune, 2006

163

4. Current Status – Biochemical Diagnosis of Human Tuberculosis. CME on

Tuberculosis by State Task Force, Sikkim, June 2008

5. Nitric Oxide, MDA levels in Serum and GPx Activity in Whole Blood of Breast

Cancer patients. 35th Annual Conference of Association of Clinical Biochemist Of

India, Kolkata, 2008

6. Attended CEZIPS, Sikkim 2009

7.Screening and laboratory diagnosis of Diabetes Mellitus , Training on NCD and

Mental Health for Medical Officers Department of Health and Human Services and

Family Welfare Department. Government of Sikkim, 17th August 2011, CRH,

Gangtok.

8. Evaluation of non enzymatic antioxidants and electrolyte status in hypertensive

patients, attending Central Referral Hospital, Gangtok. Cardiovascular Symposia.

39th Annual Conference of Association of Clinical Biochemist of India. Ranchi, 2012

9. Attended ―10th Anniversary BRAIN Disorders in the Developing world

Symposium, Bethesda, Maryland, DC‖ 11-13 Feb 2014

10. Sherpa ML, Lasopa SO, Vaddiparti K, Striley CW and Cottler LB. Does the age

of child affect drinking or depression status in the mother? Poster presented at the

103rd Annual Meeting of the American Psychopathological Association (APPA),

New York, March 3-5, 2014

11. Lasopa SO, Cottler LB, Sherpa ML and Acheampong A. Characteristics of heavy

drinking women: A community based study. Poster presentation at Research Day at

the College on Public Health and Health Professions at the University of Florida in

April 2014.

164

Experience in Educational, Research, Administration & Management

1. Member of the Cultural Committee SMIMS, 2006-2007

2. Supervisor and co-supervisor for dissertations of MSc Medical Biochemistry

3. Supervisor and co-supervisor for dissertations of MD students,

4. Co-supervisor for dissertations of DNB (Medicine) students

5. Supervisor and co-supervisor for dissertations of PhD students

6. Organizing Member for the Annual Convocations for graduates of Sikkim Manipal

University since 2008

7. Organizing member in various conferences and workshops held at SMIMS and

SMU

8. Appointed Member of SMIMS Institutional Ethics Committee in 2012.

9. NABL ASSESSOR IN MAY 2013

10. Course co-ordinator of BScMLT course, SMIMS since 2009

11. Pre Clinical ( MBBS) co-ordinator since 2013

12. Organised guest lecture on Regenerative Medicine June 2013

13. Conducted a ―Hands on Workshop on good practices of Phlebotomy by vacutainer

system‖. Trained doctors, nurses, students and technicians. June 2013

14. Attended the seminar on Bioethics at CMC Vellore, Nov 2014

15. Attended the Conclave on Asia Pacific Network of Bioethics , SRM, Chennai,

2014

Continued Medical Education (CME), Workshops and Trainings attended

1. Participated in Workshop on Youth and Promotion of Mental Health organized by

the Vishwa Yuwak Kendra Nov, 1996

165

2. Organizing member of CME on Molecular Medicine in Lady Hardinge Medical

College, New Delhi, 2003

3. Attended Regional Workshop on Telemedicine & E- Healthcare Service Delivery

SMU 2005

4. Participated as organizing member in the Regional Workshop on RNTCP- WHO,

October, SMU, 2006

5. Participated in the workshop on Scientific Writing, November, 2006

6. Participated in the workshop on ―Neonatal Advanced life Support‖ in SMU, April

2007

7. Participated in the workshop ―Pediatric Advances life Support‖ in SMU, May 2007

8. Participated in the CME- Emerging and Re-emerging infectious Diseases in India ,

SMU, 2008

9. Resource person, CME on Tuberculosis by State Task Force, Sikkim, June 2008

10. International Society of Biotechnology conference, as organizing Member. 2008

11. Organizing member of 35th Annual Conference of Indian Psychiatric Society,

Eastern Zonal Branch. (CEZIPS-2009) ―Theme: Substance abuse & Suicide‖ SMIMS

Gangtok, Sikkim.

12. Hands on workshop on Mesenchymal Stem Cells (MIRM-2011) Bangalore

13. Participated in the ―Update on Respiratory Diseases in Children‖ held in SMIMS ,

June 2011

14. Resource person for training on NCD and Mental Health for Medical Officers

Department of Health and Human Services and Family Welfare Department.

Government of Sikkim, 2011

15. ICMR: Sensitization workshop on Research Methodology: at SMU, 16-17th

October 2012

16. Attended and qualified the NABL Assessor Training course at CIPET,

Bhubaneshwar, March, 2013

166

17. Fogarty International Center, National Institutes of Health, USA, Indo-US

Training Program on Chronic Non-Communicable Diseases skills building workshop

on grant writing May, 2013

18. BD FACS Orient (BD Accuri C6) A course in Flow Cytometry Basics, August 2013,

BD-JH FACS Academy, New Delhi

19. NABL Assessors Orientation and Awareness Program (ISO15189: 2012) course at

Kolkata, August, 2013

20. Fogarty Post Doctoral Fellowship-Nov 2013-May 2014 , University of Florida,

Gainesville, Florida, USA

21. Basic Course Workshop in Medical Education Technologies, August, SMIMS,

Gangtok, 2014

22. Intellectual Property Rights protection Workshop, Gangtok, Sikkim, November 2014

167

DOCTORAL COMMITTEE

I. Air Marshal (Retd) Dr. G.S. Joneja, Dean, Administration Chairperson.

II. Dr. T.S.K.Singh , Professor & Head Microbiology, Member Secretary.

III. Dr.Selvam Ramachandran PT Associate Professor Physiotherapy, Asst.

Member Secretary.

IV. Dr. Bidita Khandelwal Professor & Head, Medicine, Member.

V. Prof. Mridula Das,Professor & Principal, SMCoN, Member.

VI. Dr. Sudip DuttaProfessor & Head, Pediatrics, Member.

VII. Dr. Sanjiba Dutta Professor & Head, Psychiatry, Member.

VIII. Dr. Luna AdhikariProfessor, Microbiology, Member.

IX. Dr. Nikita Joshi P Physiotherapy, Member.

Departmental Research Committee

X. Dr. T.A.Singh, Professor & Head, Biochemistry department.

XI. Dr.Mingma Lhamu Sherpa, Professor,Biochemistry department.

XII. Dr.S.K.Dewan,Associate Professor,Orthopedic department.

XIII. Dr.Sanjeev Dhakal,Associate Professor,Biochemistry department.

XIV. Dr.Sameer Bhandari,Associate Professor, Biochemistry department.