peptide synthesis and purification...4 biotage 13 peptide synthesis and purification biotage ®...

© Biotage 2013

Peptide Synthesis and PurificationSystems and Accessories

2 Peptide Synthesis and Purification© Biotage 2013

©2013 Biotage

Trademark Acknowledgement

Biotage, Biotage ZIP, Advancer, Advancer Kilobatch, Advancer 350, AFFINILUTE, Endeavor, EVOLUTE, EVOLUTE EXPRESS, ExploraSep, Firefly design, FLASH+, FlashMaster, FlashVac, Horizon, HPFC, HP-SIL, HP-Sphere KP-Sphere, ZIP-Sphere, Initiator, Initiator+, Initiator+ Alstra, Initiator+ Robot 8, Initiator+ Robot 60, Initiator+ SP Wave, Isolera, Isolera Prime, Isolera One, Isolera Four, Isolera LS, Isolera Dalton, Isolera Spektra, Isolera Spektra LS, Isolera Spektra One, Isolera Spektra Four, ISOLUTE, IST, IST design, KILOPREP, KP-C18-HS, KP-C18-WP, KP-C4-WP, KP-NH, KP-Sil, MIP4SPE, MIP Rule of 6, MIP[4]Process, MIP[4]Proteins, PathFinder, PRESSURE+, PRESSURE+ 48, PRESSURE+ 96, QuEChERS, RapidTrace, RapidTrace+, Resolux, Robot 8, Robot 60, Samplet, SIM, SNAP, SNAP Ultra, SP1, SP4, SP Wave, SPE Dry , SPE Dry 96, SPE Dry 96 Dual, Syro Wave, SPx, TurboVap, VacMaster, V-10, ZIF, ZIF-SIM, ZIP-Sphere, 1-Point Support.

Other product and company names mentioned herein may be trademarks or registered trademarks and/or service marks of their respective owners, and are used only for explanation and to the owners' benefit, without intent to infringe.

Delivering Innovative Technologies for Peptide Professionals

The global need for custom and synthetic therapeutic peptides is growing, as academic researchers and the pharmaceutical and biotech industries renew their interest in peptide-based drug discovery. As researchers require higher quality peptides in ever greater quantities and complexity, the need for reliable and innovative tools that offer greater productivity and increased performance is also growing.

Biotage is dedicated to meeting these needs and committed to developing the tools needed by the global peptide community for peptide synthesis and purification.

InstrumentsBiotage is the only manufacturer of traditional, room temperature peptide synthesizers as well as microwave peptide synthesizers in both entry level and high throughput configurations. We offer a range of manual, semi-automated and automated systems depending on your peptide synthesis needs. Whether you require a system that makes a single peptide or multiple branched peptides, we have an instrument to suit your requirements.

PurificationBiotage has a long history in traditional chromatographic separation and now offers Resolux™ reversed-phase HPLC columns as an ideal solution for the purification of synthetic peptides. Resolux is a range of high resolution, reversed-phase HPLC columns and guard cartridges with different packing pore size, column bonded phases and diameters available to optimize separation of any peptide.

ResinsChemMatrix® is a patented, 100% PEG (polyethylene glycol) based resin composed exclusively by primary ether bonds. The highly cross-linked PEG matrix gives the resin unmatched mechanical and chemical stability in a wide range of solvents. ChemMatrix resins have been shown to be the perfect choice for the solid phase synthesis of long, complex and hydro-phobic peptides. They are also the resin of choice for microwave assisted peptide synthesis as no leaching is observed when used with microwave peptide synthesizers at 80 °C. Peptides produced with ChemMatrix® have been shown to have higher purity and can be obtained with better yield.

ConTenTs

3 The Biotage Peptide Family4 Biotage® Initiator+ Alstra™

6 Biotage® Initiator+ SP Wave

8 Biotage® Syro Wave™

10 Syro I and Syro II12 SAP and SAM14 Biotage® Initiator Peptide Workstation15 Biotage® Resolux™

16 ChemMatrix®

18 Ordering Information

4 8

15 16

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The Biotage Peptide Family

Instrument Automation Microwave Room Temp.

Single channel

Multiple (Parallel)

Number of peptides

Scale range per reactor

vial (mmol)**UV Inert

Gas MAOS

Biotage® Initiator+ Alstra™

Full × 1 0.005-2.00

Biotage® Initiator+ SP Wave

Semi × 1 0.005-0.30 ×

Biotage® Initiator Peptide Workstation

Manual × 1 0.05-0.5 × ×

Biotage® Syro Wave™

Full 1-48

(288)* 0.001-0.30 × ×

Syro I

Full × × 1-48

(288)* 0.001-0.30 × ×

Syro II

Full × × 1-192(576)* 0.001-0.30 × ×

SAP

Semi × × 1 0.5-20 × × ×

SAM

Semi × × 1-24 0.05-0.30 × × ×

*Tip Synthesis option **Dependent on resin type and loading



Automated Microwave Peptide Synthesizer

The Biotage® Initiator+ Alstra™ is a fully automated microwave peptide synthesizer with built in flexibility for both small and large scale synthesis.

Microwave irradiation is still the most effective solution for providing a fast and precise heating method for synthesizing peptides, peptoids, PNA and peptidomimetics with higher purity and yield compared to conventional synthesis methods.

A flexible reagent setup with modular amino acid racks and reagent bottles makes the Biotage® Initiator+ Alstra™ ideal for both small and large scale synthesis. Reagents for capping and multiple coupling can also be used during a synthesis. Digital syringe pumps guarantee accurate dispensing of all reagents, and the dual needle design reduces the possibility of cross contamination. Oscillating mixing within the microwave cavity ensures homogeneous heat distribution and allows for larger scale capability up to 2 mmol.

Complete ControlThe system is controlled via the large 10" touch screen with drag and drop capability, using either pre-defined or custom methods. The built-in wizard makes sequence programming effortless, and the calculation table generated provides the quantities of reagents required.

Users can easily pause a running synthesis, check a coupling and make changes based on the result without having to reprogram the synthesis, with the additional volume of reagents required adjusted in the calculation table (“Edit on the Fly”). This avoids unnecessary double couplings.

Versatile softwareThe Branches feature is a tool that allows peptide chemists to make complex peptide modifications such as labeling of side chains, MAPs, cyclization and disulfide bridge formation.

Simplicity of operation using the versatile touch-screen interface.

The robot liquid handler dispenses concentrated liquid reagents which allows a lower excess of activated amino acids to be used.

Flexible rack set-up allows reagents to be positioned anywhere.

Five reagent bottles allowing different coupling reagents and capping steps during a synthesis.

Three external system solvents.

Accurate dispensing of reagents using digital syringe pumps.

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Any position in the sequence can be preactivated, using a designated position on the amino acid rack for premixing. The amino acid can be activated in situ or preactivated in a cycle as required.

The Biotage® Initiator+ Alstra™ can keep you informed when you are away from the instrument and send error messages or notifica-tions via e-mail after defined steps or after a completed sequence.

extended FunctionalityOptional add-on kits enable more advanced functions on the Initiator+ Alstra.

The Alstra UV Monitoring Kit allows the user to follow the removal of the Fmoc group and fully control the deprotection and coupling parameters during deprotection.

The Alstra MAOS Kit configures the system for both microwave assisted peptide and organic synthesis modes of operation with

dual mode of mixing: oscillating mixing for peptide synthesis and magnetic stirring for organic synthesis.

Advantages » Fully automated or semi-automated operation

» Branches

» Preactivation of amino acids

» “Edit on the fly”

» E-mail notification

» UV monitoring

» MAOS mode

» Synthesis on 5 µmol–2 mmol scale

» Flexible reagent setup with modular racks

» Pre-defined or custom methods

specifications

Heating ProcessTemperature range 40–100 ºCTemperature increase Typically 2–5 ºC/sPressure range Run at atmospheric pressurePower range 0–120 W at 2.45 GHzReactor vial sizes 5, 10 and 30 mLAgitation Oscillating mixing unitVial volume range 5 mL (0.6–3.5 mL)

10 mL (3.5–10 mL)30 mL (4.5–20 mL)

Inert gas (microwave cavity)

Approx. 2 L/min (0.07 cubic feet/min); 0.5 bar (0.05 MPa; 7.25 PSI)

Inert gas (manifold option)

Approx. 4 L/min (0.14 cubic feet/min); 0.5 bar (0.05 MPa; 7.25 PSI)

Liquid handlingSyringe pump 3 x digital syringe pumps, with 10 mL

sample loopFlow rate 1–50 mL/minReagent bottles 5 x 185 mL GL45 glass bottlesSolvent bottles 1 x 5 L, 1 x 2 L, and 1 x 1 L, GL45

brown glass bottlesAmino acid rack 32 x 30 mLWaste bottle 10 L

InterfacesTouch screen 10.4”Ethernet LAN Complies with IEEE 802.3

(ANSI 8802-3)USB USB 2.0Archiving/back-up Via USBPrinting Via LAN

System requirementsOperating temperature 15–35 °CStorage temperature -25 °C to 60 °CHumidity 20–75% at temperatures up to 31 °CElectrical supply Europe: 220–240 V~, 50 Hz (5 A)

US and Japan: 100–120 V~, 50/60 Hz (10 A)

Max. power consumed 1100 VAWeight 42 kg (92.6 lbs.)Dimensions (W x D x H) 640 x 430 x 640 mm

(25.2” x 16.9” x 25.2”)Vacuum pump Minimum partial pressure: 100 mbar

Minimum flow rate: 11 L/min

Certifications CE, CAN/CSA certified



Biotage® Initiator+ sP WaveMicrowave Peptide SynthesizerA versatile and programmable synthesizer that can perform microwave assisted synthesis of both peptides and organic compounds. The system delivers an industry-high 300 °C temperature and safe 30 bar pressure, making this a versatile instrument choice for any lab.

The Biotage® Initiator+ SP Wave can be used as a standalone peptide synthesizer or easily converted to a high-end MAOS system. Ideal for the synthesis of peptides, peptidomimetics, PNA and for organic, medicinal, materials, nano and polymer chemistry applications.

VersatileIn peptide synthesis mode, this is a powerful development tool for chemists synthesizing peptides and peptidomimetics, including difficult modifications and labeling of sequences, by

using single or multi-step procedures. Synthesis can be started immediately with the pre-defined methods.

By using microwave irradiation and standard reagents, this system can synthesize difficult sequences that are problematic or even impossible using conventional methods.

Complete ControlThe instrument functions are controlled directly on the touch screen interface. Deprotection and washing steps are automated, while adding activated building blocks is manual. With this approach, intermediate reaction steps are easily monitored – failure of which would only be identified upon completion of the synthesis.

Reduce CostsThe semi-automated operation reduces the consumption and waste of expensive reagents such as PNA monomers or

The intuitive interface provides complete control of the process.

The Biotage® SP Wave pump module guarantees accurate liquid dispensing using a digital syringe pump.

Perform small scale microwave synthesis by using single or multistep procedures.

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glycoamino acids that can occur in other systems due to priming or use of large dead volumes. Manual addition of exact reagent amounts minimizes waste. Save even more by benefiting from the ability to perform synthesis on a small scale from as low as 5 μmol.

Dual Mixing ModePeptide synthesis mode uses efficient vortex mixing within the microwave cavity to ensure homogeneous heat distribution. In organic synthesis mode with much higher temperature and pressure, magnetic stirring is now employed for efficient mixing.

Advantages » For peptide and organic synthesis

» Semi-automated for increased control

» Synthesis on 5–300 μmol scale

» Disposable PP-reactors for peptide synthesis

» Inert gas capability

» Use pre-defined methods or define your own

» Vortex or magnetic stirring depending on synthesis mode

» Single robot arm and digital syringe pump

optional Accessories & Consumables » PP-Reactor with PTFE frit (2, 5, and 10 mL)

» Vacuum pump ME 1C, 100–230 VAC

» Waste and scrubber kit

» Microwave vials for organic synthesis from 0.2 to 20 mL

specifications

Heating ProcessTemperature range 40–100 °CTemperature increase Typically 2–5 °C/secPressure range Run at atmospheric pressurePower range 0–100 W from magnetron at 2.45 GHzReactor vial sizes 2, 5 and 10 mLAgitation Vortex unit (500–1300 rpm)Vial volume range 2 mL reactor vial: 0.8–1.1 mL

5 mL reactor vial: 1.6–3.2 mL10 mL reactor vial: 3.2–6.4 mL

Inert gas (optional) Approximately 2 L/min (0.07 cubic feet/min), 0.5 bar (0.05 MPa; 7.25 PSI)

Liquid HandlingSyringe pump Digital single syringe pump, 11 mL

with sample loopFlow rate 1–50 mL/minReagent bottle rack 3 x 100 mLSystem solvent 1 x 2000 mL

InterfacesTouch screen 10.4”Ethernet LAN Complies with IEEE 802.3

(ANSI 8802-3)USB USB 2.0Archiving/back-up Via USBPrinting Via LAN

System RequirementsOperating temperature 18–32 °CStorage temperature -25 °C to 60 °CHumidity 20–95% at room temperatureElectrical supply Europe: 220–240 V~, 50 Hz (5 A)

US and Japan: 100–120 V~, 50/60 Hz (10 A)

Max. power consumed 1100 VAWeight 33 kg (72.7 lbs.)Dimensions (W x D x H) 400 mm x 500 mm x 685 mm

(15.7″ x 19.7″ x 27.0″)Vacuum source Minimum partial pressure: 100 mbar

Minimum flow rate: 11 L/minCertifications CE, CSA certified

Biotage® Initiator+ SP Wave


Biotage® syro Wave™

Automated Microwave and Parallel Peptide SynthesizerThe Biotage® Syro Wave™ system is the only programmable peptide synthesizer that is capable of both conventional room temperature parallel peptide synthesis and microwave assisted peptide synthesis. The system is fully automated and computer controlled utilizing a pipetting robot with a single arm.

The Biotage® Syro Wave™ is a union of the MultiSynTech Syro I and Biotage Initiator instruments, controlled by a single PC.

Syro Wave offers the unique ability to make part of the peptide sequence using conventional solid phase peptide synthesis on the parallel reactor block and then transfer the reactor vial to the microwave cavity to drive difficult couplings to completion. With the Syro Wave peptide synthesizer, Biotage offers a system with a proven pedigree and history of performance and reliability.

The Syro XP software controls the instrument functions and calculates all amounts and volumes of amino acids and

reagents required. Each cycle of the synthesis can receive a completely different protocol. This flexibility allows not only single or multiple couplings, but also complete freedom in the choice of coupling strategies within any synthesis cycle.

Intuitive Syro XP software controls instrument functions and calculates amounts and volumes of amino acids and reagents.

Microwave irradiation allows synthesis of sequences that are problematic or even impossible to synthesize using conventional methods.

Automated parallel operation allows for synthesis of more peptides in less time resulting in higher productivity.

The precise digital syringe pump guarantees low amino acid and reagent consumption, reducing coupling and running costs compared to other systems.

Intuitive Syro XP software controls instrument functions and calculates the quantities of amino acids and reagents.

Flexible reactor block formats increase synthesis throughput and result in higher productivity.

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The optional off-line cleavage and transfer process improves throughput, resulting in substantial time savings.

specifications

System SpecificationsHeating Microwave cavityAutomation Single robotic armSyringe pump Single 5 mLReactor block U-type reactor block for 2, 5 and

10 mL reactor vials. Choice of either 24 or 48 positions

Mixing Separate vortex mixers for reactor block and microwave cavity

Vacuum pump ME 2CAmino acid rack 32 x 50 mL Falcon tubesReagent bottle rack 2 x 500 mL, 3 x 200 mLWaste bottle 1 x 10 liter Nalgene bottleIncluded equipment Desktop PC, flat panel monitor,

printer and Syro XP softwareDimensions (W x D x H) 90 cm x 70 cm x 91 cmWeight 85 kg

Heating ProcessTemperature range 40–80 ºCTemperature increase Typically 2–5 ºC/secPressure range Run at atmospheric pressurePower range 0–200 W at 2.45 GHz, capped at

60 W during steady stateAgitation VortexReactor vial sizes 2, 5 and 10 mL

Microwave Unit Vial Volume Range2 mL reactor vial 0.8–1.1 mL5 mL reactor vial 1.6–3.2 mL10 mL reactor vial 3.2–6.4 mL

Power RequirementsRobot EU: 230 V~, 50 Hz (3.4 A)

USA: 120 V~, 60 Hz (6.7 A)Japan: 100 V~, 50/60 Hz (8 A)

Vacuum pump EU: 230 V~, 50/60 Hz (1.8 A)USA: 120 V~, 60 Hz (3.4 A)Japan: 100-115 V~, 50/60 Hz (3.4 A)

Biotage® Syro Wave™

Advantages » Microwave and parallel peptide synthesis

» Vortex mixing in microwave cavity and reactor block

» Single robotic arm

» Preactivation (parallel or single)

» Variable scale from 1–300 μmol

» Off-line cleavage

optional Accessories & Consumables » Inert gas option

» Inert gas cover plates

» Disposable polypropylene reactor vials

» Cleavage and Transfer Workstation

» Standalone vortex mixer for cleavage unit

» 24 and 48 position reactor blocks

» Tip synthesis module for the synthesis of 96 peptides on 1–5 μmol scale

Standalone vortex mixer for two reactor blocks, used for cleaving peptides from the resin after adding the cleavage solution.


syro I and syro IIAutomated Parallel Peptide Synthesizers

Syro I and Syro II systems are fully automated computer controlled peptide synthesizers with one or two pipetting robot arms. The modular construction offers the greatest flexibility of reaction vessels and storage containers for amino acids and reagents.

The Syro I and Syro II peptide synthesizers are the perfect choice for demanding peptide synthesis applications. Digital syringe pumps enable exact dispensing of all reactants with microliter precision. Optimal mixing of the reactants is provided by a variable speed vortex mixer. This guarantees the best synthesis results in terms of yield and purity while minimizing waste and costs.

Modular DesignDifferent reactor blocks with 24, 48 or 96 positions allow for parallel synthesis of peptides in a range from 1–300 μmol in each reaction vessel. Syro I is configured with one reactor

block and Syro II can be configured with two reactor blocks for maximum throughput and productivity.

PreactivationPreactivation of the amino acids can be performed for all the synthesis cycles or just for individual ones. The amino acids can be activated in parallel or individually directly prior to their distribution.

Tip synthesisThis option is designed for applications that require large numbers of peptides in small quantities (1–5 μmol). A Tip Synthesis Module can make up to 96 peptides at a time. Each peptide is synthesized in a 0.4 ml tip with PE frit where accurate dispensing of reagents is essential. The Syro I system can accommodate 1–3 Tip Synthesis Modules, and the Syro II system can accommodate 3–6 modules, allowing up to 576 peptides to be made at the same time.

Syro I Syro II

Accurate dispensing of exact volumes of all reactants and vortexing of the reaction mixture guarantee the best synthesis results.

Automated parallel operation allows for the synthesis of more peptides in shorter times. Flexible reactor block formats increase synthesis throughput and result in the highest productivity.

The unique U-block reactor assembly has a truly independent flow path for each reactor vial with no possibility of cross-contamination.

3-tip washcomb for faster washing and dispensing of reagents.

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Advantages » Single or dual robotic arms

» Customizable reactor blocks with 24, 48 or 96 positions

» Disposable amino acid containers

» Variable scale from 1–300 μmol

» Off-line cleavage

optional Accessories » Inert gas option for reagent containers,

amino acid rack and reactor vials

» Inert gas cover plates

» Disposable polypropylene reactor vials


» Standalone vortex mixer for cleavage unit

» Reactor blocks

» Tip Synthesis Module

specifications

System Specification Syro I Syro IIAutomation Single robotic arm Dual robotic arm with a triple pipetting washcombLiquid handling Two 5 mL digital syringe pumps Four digital syringe pumps*Mixing Vortex mixer for reactor block Vortex mixer for two reactor blocksReactor block U-type reactor block for 2, 5 and 10 mL reactor vials.

Choice of either 24 or 48 positionsTwo U-type reactor blocks for 2, 5 and 10 mL reactor vials. Choice of either 24 or 48 positions

Vacuum pump ME 2C ME 4CAmino acid rack 40 x 50 mL Falcon tubes 40 x 50 mL Falcon tubesReagent bottle rack 2 x 500 mL, 3 x 200 mL 3 x 500 mL, 4 x 200 mLWaste bottle 1 x 10 liter Nalgene bottle 1 x 20 liter Nalgene bottleEquipment Includes desktop PC, flat panel monitor, printer, and

Syro XP softwareIncludes desktop PC, flat panel monitor, printer and Syro XP software

Dimensions (W x D x H) 56 cm x 70 cm x 91 cm 82 cm x 70 cm x 91 cmWeight 60 kg 85 kg

Power RequirementsRobot (max. 250 W) EU:230 V~, 50 Hz (1.5 A)

USA: 120 V~, 60 Hz (2.5 A)Japan: 100 V~, 50/60 Hz (3.0 A)

Vacuum pump EU: 230 V~, 50/60 Hz (1.8 A)USA: 120 V~, 60 Hz (3.4 A)Japan: 100–115 V~, 50/60 Hz (3.4 A)

* 1 x 5 mL digital syringe pump for amino acid addition, 3 x 10 mL digital syringe pumps for solvent wash and reagent addition.

Disposable polypropylene reactor vials with PTFE frits. Available in 2 mL, 5 mL and 10 mL sizes.

Optional Tip Synthesis System for applications that require large number of peptides in small quantities (1–5 μmol). This is composed of three modules: Synthesis module, Split-off module and Cleavage module.

Syro I and Syro II


The SAP (Semi-Automated Single Peptide Synthesizer) and SAM (Semi-Automated Multiple Peptide Synthesizer) are user-friendly synthesizers with a high level of flexibility.

SAP and SAM are operated using an external controller with an LCD display and six function keys. The controller is used for single commands (i.e. FILL, REACT, EMPTY) as well as for creation and execu-tion of entire complex programs, and it can store 10-programs with up to 20 steps each.

sAP and sAMSemi-Automated Synthesizers of Single or Multiple Peptides

SAP SAM

Digital syringe pump and eight-way valve ensures accurate dispensing of reagents.

The controller has a very simple design with a LCD display and function buttons.

Vortex mixer for effective mixing.

0.5–20 mmol scale synthesisSynthesize up to 24 peptides at a time.

single PeptideSAP is a semi-automated peptide synthesizer ideal for the synthesis of single peptides, operated using an external controller. The synthesis is performed in a glass reaction vessel with glass frit with a scale that can be varied from 0.5–20 mmol.

Seven individual solvents and reagents can be pumped into the reaction flask via an eight-way valve (right). A digital syringe pump provides accurate dispensing of reagents. The sprinkler head is specially constructed to allow splash-free addition of the reagents, spraying of the wash solvent and also cleaning of the flask. The variable shaker guarantees homogeneous mixing of the reactants.

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Multiple PeptidesSAM is a semi-automated multiple (parallel) peptide synthe-sizer producing up to 24 peptides in parallel. The 24-position U-block reactor can be operated with 2 mL, 5 mL, or 10 mL disposable polypropylene reactors (with PTFE frits) for Fmoc-synthesis between 5 and 300 μmol per reactor vial. The U-type reactor block and reactor vials are the same used on the fully automated systems Syro I and II.

Four individual solvents and reagents can be pumped into the reaction flask. A digital syringe pump provides accurate dispensing of reagents while a special dispensing plate allows the reagents and solvents to be distributed into the reactor vials. The liquid distribution proceeds in parallel for 6 reactors at a time, with the variable vortexer guaranteeing homogeneous mixing of the reactants.

Advantages » Compact footprint

» Cost-effective

» Variable synthesis scales

» Digital syringe pumps enable precise addition of reagents

optional Accessories

sAP only » Glass reactors with glass frit

sAM only » Disposable polypropylene reactor vials


» Vortex mixer for cleavage unit

specifications

System Specifications SAP SAMLiquid handling 10 mL digital syringe pump 10 mL digital syringe pumpLiquid ports 8-way valve with 1 outlet port and

7 reagents/solvents ports8-way valve with 4 outlet ports and 4 reagents/solvents ports

Valve 3-way PTFE-valve 3-way PTFE-valveMixing Shaker 24 V, 30–300 rpm Vortex mixer 24 V, 100–600 rpmLiquid dispensing Sprinkler head Dispensing plateVessel holder Single vessel stand 24 position U-type reactor blockReaction vessels Glass reactor with glass frits. 250 mL as standard and

25 mL, 50 mL, 100 mL and 500 mL optional reaction vessels

PP-reactors with PTFE-frits 2 mL, 5 mL, 10 mL

Synthesis scale 0.5–20 mmol 0.05–0.3 mmol

VacuumVacuum pump ME 2C ME 2CVacuum waste container 10 L Nalgene bottle 10 L Nalgene bottle

ControllerType Microprocessor MicroprocessorDisplay LC-display with 40 x 16 characters LC-display with 40 x 16 charactersPresets 10 program files with 20 steps.

Automatic fill and wash procedures10 program files with 20 steps. Automatic fill and wash procedures

Manual mode Yes Yes

Power RequirementsInput voltages 10–220 VAC +/- 10 % 10–220 VAC +/- 10 %Power supply 24 V max. 150 W 24 V max. 150 W

Dimensions (W x D x H)Synthesizer 460 mm x 250 mm x 360 mm 540 mm x 250 mm x 345 mmController 195 mm x 65 mm x 170 mm 195 mm x 65 mm x 170 mm

SAP and SAM


Biotage® Initiator Peptide WorkstationManual Microwave Peptide Synthesis

A cost effective and versatile accessory for manual microwave peptide synthesis and cleavage when used with a Biotage® Initiator+ microwave synthesizer.

The workstation can be used to perform various types of chemistry including solution phase and solid phase peptide synthesis, organic and PNA synthesis. Reactions are performed in the microwave peptide vial under atmospheric conditions at temperatures up to 100 °C in an Initiator. The vial contents are quickly filtered and washed using the wash station.

Reusable snap cap with septum.

Check valve allows for easy emptying of the vial when inserted in the vacuum head.

Attach a vacuum source to the vacuum head for fast and easy emptying.

Material can be collected in a standard GL 45 laboratory glass bottle (shown right), 50 mL centrifuge tube (shown left) or a round bottom flask.

The microwave peptide vial is available in two different sizes; 2–5 mL and 10–20 mL for synthesis on 50–500 μmol scale respectively.

Initiator Peptide Workstation

Advantages » Entry-level microwave peptide synthesis accessory

» For use on Biotage® Initiator systems without modifications

» Fast emptying and washing for rapid cycle times

» Perform solution and solid phase microwave peptide synthesis

» Reusable 2–5 mL or 10–20 mL microwave peptide vials

» Synthesis on 50–500 μmol scale

» Suitable for PNA and peptoid synthesis

optional Accessories » 2–5 mL microwave peptide vial barrel

» 10–20 mL microwave peptide vial barrel

» Check valve o-ring

» Snap cap

» Septum

» Disposable HDPE frit

Chemists can now easily access the benefits of microwave assisted peptide synthesis using the Biotage® Initiator+ with the Biotage® Initiator Peptide Workstation.

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Biotage® Resolux™

HPLC Columns for Purification of Synthetic Peptides

Biotage has applied its long history in traditional chromatographic separation to Resolux reversed-phase HPLC columns and can now offer an ideal solution for purifying synthetic peptides. The Resolux HPLC columns provide excellent resolution and are an economical choice for the peptide chemist.

Purifying peptides from crude synthetic mixtures can be extremely challenging. The Biotage® Resolux™ range of high resolution reversed-phase HPLC columns and guard cartridges with different packing pore size, column bonded phases and diameters are available to optimize separation of any peptide.

All Resolux products are available as analytical, semi-prepar-ative and preparative HPLC columns, ranging from 2.1 mm to

20 mm diameter for predictable scaleup. HPLC guard cartridges for in-line front-end protection of all HPLC columns are also available. Optimized phase bonding and column packing techniques ensure unsurpassed performance and column-to-column reproducibility and reliability.

Advantages » High resolution reversed-phase HPLC

columns and guard cartridges

» Analytical, semi-preparative and preparative columns in 3 different lengths (100 mm, 150 mm, 250 mm)

» C4, C8 and C18 bonded phases for improved selectivity

» Separation of peptides differing by as little as one amino acid

» Three different pore sizes; 120 Å, 200 Å, 300 Å

» Economical while delivering exceptional performance and unrivaled efficiency in demanding situations

See also "Specifications Biotage® Resolux™" table on page 21–22.

Guard cartridges are available for all scales: analytical scale with 4.5 μm particle size, semi-preparative and preparative scale with either 4.5 or 10 μm particle size.

Semi-preparative and preparative columns with internal diameters of 10 mm and 20 mm. Each of these columns are available in lengths of 100, 150 and 250 mm.

Choice of three analytical columns with different internal diameters; 2.1, 3.0 and 4.6 mm. Each column is available in three different lengths; 100 mm, 150 mm and 250 mm.

Biotage® Resolux™


ChemMatrix®

Resin Technology Opening New Possibilities for Peptide Drug Research

ChemMatrix® is a patented, 100% PEG (polyethylene glycol) based resin that offers substantial advantages over traditional PEG based polystyrene resins for solid phase peptide synthesis. ChemMatrix helps chemists make longer peptides with higher purity and yield.

ChemMatrix® resin, developed by PCAS, is composed exclu-sively of primary ether bonds that are highly cross-linked, forming the PEG matrix. This composition makes the resin mechanically and chemically stable in the presence of a wide range of solvents. ChemMatrix overcomes the challenges of synthesizing longer and more complex therapeutic peptides. Peptides produced with ChemMatrix have higher purity and can be obtained with better yield.

Advantages » Exceptional stability

» Superior properties

» High loading

» Solvent compatibility

» Versatile choices

» Pre-loaded options (on request)*

» Microwave compatibility

Complete Worldwide support » Extensive polymer knowledge

» Full technical support

» Uncompromising product quality

» Available in all quantities, from research to industrial scale*Subject to minimum order quantity.

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ProductRink Amide-ChemMatrix® HMPB-ChemMatrix® Trityl-ChemMatrix® Wang-ChemMatrix® PAL-ChemMatrix®

Description Rink Amide-ChemMatrix is one of the most popular resins for both manual and automated SPPS, under microwave or non-microwave conditions. This is an excellent support for the synthesis of peptide amides and is compatible with our range of microwave peptide synthesizers such as the Biotage® Syro Wave™ or Initiator+ Alstra.

For peptide acids, we recommend using the HMPB-ChemMatrix as this resin will provide high crude purity and a recovery yield of 90–95%. The Wang-ChemMatrix will produce similar crude peptide purity, but the recovery yield is lower (60–70%).

Trityl-ChemMatrix is mainly used to obtain a protected peptide as it requires very low TFA cleavage conditions (under 1%). This resin therefore uses the same cleavage conditions as the Cl-Trityl-polystyrene. Please note that the Cl-Trityl-ChemMatrix resin is not offered for stability reasons. It is more effective to start with the Trityl-ChemMatrix resin, chlorinate the resin and then add the amino acid required.

Wang ChemMatrix was the first ChemMatrix resin developed for peptide acids. While the purity of peptides synthesized with this resin are excellent, the recovery yield is limited to 60–70%. For enhanced yields and higher recoveries (90–95%), we recommend using the HMPB-ChemMatrix.

PAL-ChemMatrix is also an excellent support for the synthesis of peptide amides and is highly recommended for microwave synthesis. There is some evidence to suggest that PAL ChemMatrix can provide higher purity peptide amides.

Category Peptide amide resin Peptide acid resin Peptide acid resin Peptide acid resin Peptide amide resin

Cleavage conditions

TFA/H2O/Et3SiH (95/2.5/2.5)

TFA/H2O/Et3SiH (95/2.5/2.5)

TFA < 1% TFA/H2O/Et3SiH (95/2.5/2.5)

TFA/H2O/Et3SiH (95/2.5/2.5)

Loading 0.4–0.6 mmol/g 0.4–0.6 mmol/g 0.40–0.65 mmol/g 0.40–0.65 mmol/g 0.4–0.5 mmol/g

Bead size 100–200 mesh dry 100–200 mesh dry 100–200 mesh dry 100–200 mesh dry 100–200 mesh dry

ChemMatrix®

Hints and TipsChemMatrix resins may swell more than polystyrene resins especially during the cleavage step where high concentrations of TFA are used. It is important to use a reaction vessel of suitable volume to accommodate this normal swelling during use.

Comparison of ChemMatrix® and polystyrene swelling profiles in a variety of SPPS solvents. The increased swelling in the case of ChemMatrix confers much greater solvent and reagent accessibility and thereby reduced the occurrence of by products or impurities such as incomplete couplings and deletion sequences.



Product Part Number

Initiator+ Alstra Microwave Peptide Synthesizer 220–240 V

356017

Initiator+ Alstra Microwave Peptide Synthesizer 110–120 V

356018

Optional Accessories and ConsumablesInert gas manifold 3562085 mL Reactor vial with PTFE frit (50/pk) 35628810 mL Reactor vial with PTFE frit (50/pk) 35628930 mL Reactor vial with PTFE frit (50/pk) 3562905 mL Reactor vial extension (5/pk) 35629110 mL Reactor vial extension (5/pk) 35622130 mL Reactor vial extension (5/pk) 35622210 mL Amino acid tubes (100/pk) 35623930 mL Amino acid tubes (100/pk) 35624050 mL Amino acid tubes (100/pk) 35624120 x 10 mL Amino acid rack 35616224 x 30 mL Amino acid rack 35616732 x 30 mL Amino acid rack 35619328 x 50 mL Amino acid rack 35619820 x 10 mL Rack cover plate 35616324 x 30 mL Rack cover plate 35616832 x 30 mL Rack cover plate 35619428 x 50 mL Rack cover plate 356199R1-4 reagent bottle cover plate 356158R5 reagent bottle cover plate 35620320 x 10 mL Foil septa (5/pk) 35616624 x 30 mL Foil septa (5/pk) 35619232 x 30 mL Foil septa (5/pk) 35619728 x 50 mL Foil septa (5/pk) 356202R1-4 reagent bottle foil septa (5/pk) 356161R5 reagent bottle foil septa (5/pk) 3562065 mL/10 mL Reactor vial caps (50/pk) 35625230 mL Reactor vial caps (50/pk) 356253Reactor vial plugs (50/pk) 356292185 mL Reagent bottle, glass 356254Vacuum pump ME1C, 100-230 VAC 50-60 Hz 356330

ServiceInstallation and training SER-IA-INService Agreement - Priority - Alstra SER-IA-SAPPeptide Application Training – 1 day SER-PEP-AT

Biotage® Initiator+ sP Wave

Product Part Number

Initiator+ SP Wave (EU) 356014Initiator+ SP Wave (USA/JPN) 356015

Optional Accessories and ConsumablesPP-Reactor, 2 mL, with PTFE frit, 100/pk V020TF051PP-Reactor, 5 mL, with PTFE frit, 100/pk V050TF062

Product Part Number

PP-Reactor, 10 mL, with PTFE frit, 100/pk V100TF086Vacuum pump ME1C, 100-230 VAC 50-60 Hz 356330Waste and scrubber kit 356016Microwave Vials 0.2–0.5 mL, 100/pk 355458Microwave Vials 0.5–2 mL, 100/pk 352016Microwave Vials 2–5 mL, 100/pk 351521Microwave Vials 10–20 mL, 50/pk 354833Cap 10 mL vial 355838Cap 5 mL vial 355839Cap 2 mL vial 355918

ServiceInstallation and training SER-ISPW-INService Agreement - Priority - SP Wave SER-SPW-SAPPeptide Application Training – 1 day SER-PEP-AT

Biotage® syro Wave™

Product Part Number

Microwave and Parallel Peptide Synthesizer 48 x 2 mL reactor block

60 Hz, 115 VAC (USA)No options S1MP-1A-X-XInert Gas S1MP-1A-IG-XInert Gas, Tip Synthesis S1MP-1A-IG-TSTip Synthesis S1MP-1A-X-TS

50 Hz, 230 VAC (UK/EU)No options S1MP-2A-X-XInert Gas S1MP-2A-IG-XInert Gas, Tip Synthesis S1MP-2A-IG-TSTip Synthesis S1MP-2A-X-TS

50/60 Hz, 100 VAC (JPN)No options S1MP-3A-X-XInert Gas S1MP-3A-IG-XInert Gas, Tip Synthesis S1MP-3A-IG-TSTip Synthesis S1MP-3A-X-TS

Microwave and Parallel Peptide Synthesizer 24 x 5 mL reactor block

60 Hz, 115 VAC (USA)No options S1MP-1B-X-XInert Gas S1MP-1B-IG-XInert Gas, Tip Synthesis S1MP-1B-IG-TSTip Synthesis S1MP-1B-X-TS

50 Hz, 230 VAC (UK/EU)No options S1MP-2B-X-XInert Gas S1MP-2B-IG-XInert Gas, Tip Synthesis S1MP-2B-IG-TS

ordering Information

19

Product Part Number

Tip Synthesis S1MP-2B-X-TS

50/60 Hz, 100 VAC (JPN)No options S1MP-3B-X-XInert Gas S1MP-3B-IG-XInert Gas, Tip Synthesis S1MP-3B-IG-TSTip Synthesis S1MP-3B-X-TS

ServiceService, installation and initial training SER-MP-INService Agreement - Priority - Syro Wave SER-SYW-SAPPeptide Application Training – 1 day SER-PEP-AT

syro I

Product Part Number

Parallel Peptide Synthesizer 48 x 2 mL reactor block

60 Hz, 115 VAC (USA)No options S1PS-1A-X-XInert Gas S1PS-1A-IG-XInert Gas, Tip Synthesis S1PS-1A-IG-TSTip Synthesis S1PS-1A-X-TS

50 Hz, 230 VAC (UK/EU)No options S1PS-2A-X-XInert Gas S1PS-2A-IG-XInert Gas, Tip Synthesis S1PS-2A-IG-TSTip Synthesis S1PS-2A-X-TS

50/60 Hz, 100 VAC (JPN)No options S1PS-3A-X-XInert Gas S1PS-3A-IG-XInert Gas, Tip Synthesis S1PS-3A-IG-TSTip Synthesis S1PS-3A-X-TS

Parallel Peptide Synthesizer 24 x 5 mL reactor block

60 Hz, 115 VAC (USA)No options S1PS-1B-X-XInert Gas S1PS-1B-IG-XInert Gas, Tip Synthesis S1PS-1B-IG-TSTip Synthesis S1PS-1B-X-TS

50 Hz, 230 VAC (UK/EU)No options S1PS-2B-X-XInert Gas S1PS-2B-IG-XInert Gas, Tip Synthesis S1PS-2B-IG-TSTip Synthesis S1PS-2B-X-TS

50/60 Hz, 100 VAC (JPN)No options S1PS-3B-X-XInert Gas S1PS-3B-IG-XInert Gas, Tip Synthesis S1PS-3B-IG-TS

Product Part Number

Tip Synthesis S1PS-3B-X-TS

ServiceService, installation and initial training SER-PS1-INService Agreement - Priority - Syro I SER-SY1-SAPPeptide Application Training – 1 day SER-PEP-AT

syro II

Product Part Number

Parallel Peptide Synthesizer 2 (48 x 2 mL) reactor blocks

60 Hz, 115 VAC (USA)No options S2PS-1A-X-XInert Gas S2PS-1A-IG-XInert Gas, Tip Synthesis S2PS-1A-IG-TSTip Synthesis S2PS-1A-X-TS50 Hz, 230 VAC (UK/EU)No options S2PS-2A-X-XInert Gas S2PS-2A-IG-XInert Gas, Tip Synthesis S2PS-2A-IG-TSTip Synthesis S1PS-2A-X-TS

50/60 Hz, 100 VAC (JPN)No options S2PS-3A-X-XInert Gas S2PS-3A-IG-XInert Gas, Tip Synthesis S2PS-3A-IG-TSTip Synthesis S2PS-3A-X-TS

Parallel Peptide Synthesizer 2 (24 x 5 mL) reactor blocks

60 Hz, 115 VAC (USA)No options S2PS-1B-X-XInert Gas S2PS-1B-IG-XInert Gas, Tip Synthesis S2PS-1B-IG-TSTip Synthesis S2PS-1B-X-TS

50 Hz, 230 VAC (UK/EU)No options S2PS-2B-X-XInert Gas S2PS-2B-IG-XInert Gas, Tip Synthesis S2PS-2B-IG-TSTip Synthesis S2PS-2B-X-TS

50/60 Hz, 100 VAC (JPN)No options S2PS-3B-X-XInert Gas S2PS-3B-IG-XInert Gas, Tip Synthesis S2PS-3B-IG-TSTip Synthesis S2PS-3B-X-TS

ServiceService, installation and initial training SER-PS2-INService Agreement - Priority - Syro II SER-SY2-SAPPeptide Application Training – 1 day SER-PEP-AT

Ordering Information


sAP

Product Part Number

115 VAC, 60 Hz (USA) S3PS-1230 VAC, 50 Hz (UK/EU) S3PS-2100 VAC, 50/60 Hz (JPN) S3PS-3Service, installation and initial training SER-PS3-INService Agreement - Priority - SAP SER-SAP-SAP

sAM

Product Part Number

115 VAC, 60 Hz (USA) S4PS-1230 VAC, 50 Hz (UK/EU) S4PS-2100 VAC, 50/60 Hz (JPN) S4PS-3Service, installation and initial training SER-PS4-INService Agreement - Priority - SAM SER-SAM-SAP

Initiator Peptide Workstation

Product Pack Qty. Part Number

Initiator Peptide Workstation, 2–5 mL vial (355964 + 355968)

355985

Initiator Peptide Workstation, 10–20 mL vial (355965 + 355968)

355986

2–5 mL Microwave peptide vial (barrel, check valve, frit, snap cap, and septum) and additional septum (5), frit (5), and check valve o-ring (2)

355964

10–20 mL Microwave peptide vial (barrel, check valve, frit, snap cap, and septum) and additional septum (5), frit (5), and check valve o-ring (2)

355965

InstrumentsInitiator Microwave System (EU) 355301Initiator Microwave System (US/JPN) 355302Initiator+ Microwave System (EU) 356006Initiator+ Microwave System (US/JPN) 356007

ConsumablesWash Station 1 3559681000 mL GL 45 lab glass bottle 1 3558142–5 mL Microwave peptide vial barrel 1 35578210–20 mL Microwave peptide vial barrel

1 355803

Check valve 1 355957Check valve o-ring 2 355962Snap cap 1 355804Septum 5 355960Disposable HDPE frit 5 355961Magnetic stirring bar for 2–5 mL vials 5 355543Magnetic stirring bar for 10–20 mL vials

5 353930

Vacuum seal, kalrez 1 355959Vacuum seal, viton 5 355966Rubber feet 4 355967

Accessories

Product Part Number

Inert Gas Cover Plates24 x 2 mL reactor Z002IC02424 x 5 mL reactor Z005IC02424 x 10 mL reactor Z010IC02448 x 2 mL reactor Z002IC04896 pos. for Tip-reactors Z004IC096

Cleavage and Transfer UnitsTransfer Unit 24 position S011TU024Transfer Unit 48 position S011TU048Transfer Unit 24-position & 48-position S011TU424Vortex mixer, stand-alone, for two U-block reactors during cleavage

S006VO002

U-Block Reactors and Empty HeadsU-block Reactor, 24 x 2-5-10 mL Z024UB050U-block Reactor, 48 x 2 mL Z048UB020U-block Reactor, 96 x 2 mL (Syro II only) Z096UB020Empty Head, 24-position Z024AK000Empty Head, 48-position Z048AK000Empty Head, 96-position (Syro II only) Z096AK000

Glass Reaction Vessel for SAP500 mL Z500RK003250 mL Z250RK003100 mL Z100RK00350 mL Z050RK00325 mL Z025RK003

Consumables

Product Pack Qty. Part Number

PP-Reactor, 2 mL, with PTFE frit 100 V020TF051PP-Reactor, 5 mL, with PTFE frit 100 V050TF062PP-Reactor, 10 mL, with PTFE frit 100 V100TF086PP-Reactor Tip, 0.4 mL with PE Frit 96 V004PE050PP-Reactor, 10 mL, with PTFE frit (for Inert Gas)

100 V100TF073

Luer Stoppers 100 V000LS100

ChemMatrix Resins

Resin Volume Part Number

HMPB-ChemMatrix 5 g 7-820-1310-510 g 7-820-1310-1025 g 7-820-1310-25

PAL-ChemMatrix 5 g 7-550-1310-510 g 7-550-1310-1025 g 7-550-1310-25

Wang-ChemMatrix 5 g 7-320-1310-510 g 7-320-1310-1025 g 7-320-1310-25

21

Resin Volume Part Number

Trityl-ChemMatrix 5 g 7-420-1310-510 g 7-420-1310-1025 g 7-420-1310-25

Rink Amide-ChemMatrix 5 g 7-600-1310-510 g 7-600-1310-1025 g 7-600-1310-25

Resolux Guard Cartridge Holders

Holder Size (mm) Connection Pack

Qty. Part Number

Guard holder analytical FM

20 X 3.2 Female/Male 1 R4-2032-GHOLD

Guard holder analytical FF

20 X 3.2 Fem/Fem 1 R4-2032-GHFF

Guard holder semi-prep

30 X 10 Fem/Fem 1 R4-3010-GHOLD

Guard holder preparative

25 X 20 Fem/Fem 1 R4-2520-GHOLD

Resolux

Product Phase Size (mm)

Dia. (μm)

Pack Qty. Part Number

Resolux 120 GuardsAnalytical guard

C8 20 X 2.1 4.5 5 R1-0221-1085

20 X 3.2 4.5 5 R1-0232-1085C18 20 X 2.1 4.5 5 R1-0221-1185

20 X 3.2 4.5 5 R1-0232-1185Semi-preparative guard

C8 30 X 10 4.5 2 R1-0310-1085

C18 30 X 10 10 2 R1-0310-1181Preparative guard

C8 25 X 20 4.5 2 R1-0220-1085

C18 25 X 20 10 2 R1-0220-1181

Resolux 120 Columns/CartridgesAnalytical column

C8 100 X 2.1 4.5 1 R1-1021-1085

150 X 2.1 4.5 1 R1-1521-1085250 X 2.1 4.5 1 R1-2521-1085100 X 3.0 4.5 1 R1-1030-1085150 X 3.0 4.5 1 R1-1530-1085250 X 3.0 4.5 1 R1-2530-1085100 X 4.6 4.5 1 R1-1046-1085150 X 4.6 4.5 1 R1-1546-1085250 X 4.6 4.5 1 R1-2546-1085

C18 100 X 2.1 4.5 1 R1-1021-1185150 X 2.1 4.5 1 R1-1521-1185250 X 2.1 4.5 1 R1-2521-1185100 X 3.0 4.5 1 R1-1030-1185150 X 3.0 4.5 1 R1-1530-1185250 X 3.0 4.5 1 R1-2530-1185


Dia. (μm)


100 X 4.6 4.5 1 R1-1046-1185150 X 4.6 4.5 1 R1-1546-1185250 X 4.6 4.5 1 R1-2546-1185

Semi-preparative

C8 100 X 10 4.5 1 R1-1010-1085

150 X 10 4.5 1 R1-1510-1085250 X 10 4.5 1 R1-2510-1085

C18 100 X 10 4.5 1 R1-1010-1185150 X 10 4.5 1 R1-1510-1185250 X 10 4.5 1 R1-2510-1185

Preparative C8 100 X 20 4.5 1 R1-1020-1085150 X 20 4.5 1 R1-1520-1085250 X 20 4.5 1 R1-2520-1085

C18 100 X 20 4.5 1 R1-1020-1185150 X 20 4.5 1 R1-1520-1185250 X 20 4.5 1 R1-2520-1185


C4 20 X 2.1 4.5 5 R2-0221-2045

20 X 3.2 4.5 5 R2-0232-2045C18 20 X 2.1 4.5 5 R2-0221-2185


C4 30 x 10 10 2 R2-0310-2041


C4 25 X 20 10 2 R2-0220-2041

C18 25 X 20 10 2 R2-0220-2181

Resolux 200 Columns/CartridgesAnalytical column

C4 100 X 2.1 4.5 1 R2-1021-2045

150 X 2.1 4.5 1 R2-1521-2045250 X 2.1 4.5 1 R2-2521-2045100 X 3.0 4.5 1 R2-1030-2045150 X 3.0 4.5 1 R2-1530-2045250 X 3.0 4.5 1 R2-2530-2045100 X 4.6 4.5 1 R2-1046-2045150 X 4.6 4.5 1 R2-1546-2045250 X 4.6 4.5 1 R2-2546-2045

C18 100 X 2.1 4.5 1 R2-1021-2185150 X 2.1 4.5 1 R2-1521-2185250 X 2.1 4.5 1 R2-2521-2185100 X 3.0 4.5 1 R2-1030-2185150 X 3.0 4.5 1 R2-1530-2185250 X 3.0 4.5 1 R2-2530-2185100 X 4.6 4.5 1 R2-1046-2185150 X 4.6 4.5 1 R2-1546-2185250 X 4.6 4.5 1 R2-2546-2185

Semi-preparative

C4 100 X 10 4.5 1 R2-1010-2045

150 X 10 4.5 1 R2-1510-2045250 X 10 4.5 1 R2-2510-2045

C18 100 X 10 4.5 1 R2-1010-2185150 X 10 4.5 1 R2-1510-2185250 X 10 4.5 1 R2-2510-2185

Ordering Information



Dia. (μm)



C18 100 X 20 4.5 1 R2-1020-2185150 X 20 4.5 1 R2-1520-2185250 X 20 4.5 1 R2-2520-2185


C4 20 X 2.1 4.5 5 R3-0221-3045

20 X 3.2 4.5 5 R3-0232-3045C18 20 X 2.1 4.5 5 R3-0221-3185


C4 30 X 10 10 2 R3-0310-3041


C4 25 X 20 10 2 R3-0220-3041

C18 25 X 20 10 2 R3-0220-3181

Resolux 300 Columns / CartridgesAnalytical column

C4 100 X 2.1 4.5 1 R3-1021-3045

150 X 2.1 4.5 1 R3-1521-3045250 X 2.1 4.5 1 R3-2521-3045100 X 3.0 4.5 1 R3-1030-3045150 X 3.0 4.5 1 R3-1530-3045


Dia. (μm)


250 X 3.0 4.5 1 R3-2530-3045100 X 4.6 4.5 1 R3-1046-3045150 X 4.6 4.5 1 R3-1546-3045250 X 4.6 4.5 1 R3-2546-3045

C18 100 X 2.1 4.5 1 R3-1021-3185150 X 2.1 4.5 1 R3-1521-3185250 X 2.1 4.5 1 R3-2521-3185100 X 3.0 4.5 1 R3-1030-3185150 X 3.0 4.5 1 R3-1530-3185250 X 3.0 4.5 1 R3-2530-3185100 X 4.6 4.5 1 R3-1046-3185150 X 4.6 4.5 1 R3-1546-3185250 X 4.6 4.5 1 R3-2546-3185

Semi-preparative

C4 100 X 10 4.5 1 R3-1010-3045

150 X 10 4.5 1 R3-1510-3045250 X 10 4.5 1 R3-2510-3045

C18 100 X 10 4.5 1 R3-1010-3185150 X 10 4.5 1 R3-1510-3185250 X 10 4.5 1 R3-2510-3185


C18 100 X 20 4.5 1 R3-1020-3185150 X 20 4.5 1 R3-1520-3185250 X 20 4.5 1 R3-2520-3185

specifications Biotage® Resolux™

Resolux 120 Resolux 200 Resolux 300

Backbone Silica Silica SilicaPhase C8, C18 C4, C18 C4, C18Particle size 4.5 μm, spherical 4.5 μm, spherical 4.5 μm, sphericalPore size 120 Å 200 Å 300 ÅPore volume 0.8 mL/g 1.1 mL/gm 0.9 mL/gmSurface area 330 m2/g 200 m2/gm 100 m2/gmCarbon content C8 = 10%; C18 = 18% C4 = 4.5%; C18 = 14% C4 = 3%; C18 = 8%Phase type Fully end-capped monofunctional

phasesFully end-capped monofunctional phases

Fully end-capped monofunctional phases

© Biotage 2013

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