PEGASUS ® GC-HRT + 4D

The solution for complex sample analysis has arrived. Our PEGASUS ® GC-HRT+ 4D combines the highest performance Time-of-Flight mass spectrometer with industry leading GC×GC, giving analytical scientists the ability to identify components with more confidence, convert unknowns to real knowns, and find out what else is in their sample. The Pegasus GC-HRT+ 4D takes advantage of four dimensions of separation and resolution.

• 1st dimension chromatographic resolution

• 2nd dimension chromatographic resolution

• High mass resolution and exceptional mass accuracy

• High Resolution Deconvolution® (HRD ®) from the leaders in deconvolution

The Ultimate GC×GC Game Changing Technology

®®HHRRDD

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• A combination of the highest performance GC×GC and TOF on the market gives users an unprecedented ability to interrogate complex samples

• Find more analytes than ever before and identify components with the ultimate confidence

• Mass accuracies of 1 ppm and peak capacities at least two times greater than any other product in the marketplace

• The industry’s most established GC×GC system; thermal modulation with liquid nitrogen or cryogen-free versions

• Novel Encoded Frequent Pushing® (EFP ®) allows increased sensitivity

• Chemical Ionization Source (HR-CI) data has the same mass accuracy and high resolution on pseudo- molecular ions, which complements the traditional Electron Ionization Source (HR-EI), to provide the comprehensive characterisation of unknowns

• Integrated software platform acquires data, controls all hardware, and analyses and reports results with a high level of automation; tailored to get the most out of High Resolution TOF data

• Designed to maintain 1st dimension chromatographic separation and harness the power of a 2nd dimension of chromatography

An Integration of Two High Performance Technologies

+

KADAS – Patented acquisition system, which dramatically reduces noise, ensures mass accuracy, and reduces file size

New Encoded Frequent Pushing™ – a patented method of pulsing the Orthogonal Accelerator multiple times to increase duty cycle (x10)System designed for very fast

acquisition rates needed to define narrow second dimension peaks

® ®Folded Flight Path (FFP ) provides high order TOF focusing which delivers high resolution sensitivity with two modes of operation (25k and 50k mass resolution)

HR-EI or HR-CI sources

Carrier Gas Flow

Sampling Inlet

CapillaryColumn

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Main Oven

Secondary Oven

Thermal Modulator

st1 Dimension Column

Gas Chromatograph

Detector

Periodic Ion LensesGridless Mirror

Gridless Mirror

Note: Not to Scale

New EI Ion Sources optimized for GCxGC performance

Ÿ Chromatographic symmetry is quantified with peak tailing calculation

Ÿ HR-EI compatible with both H 2

and He carrier gases

Conventional HR-CI source also available with superior GCxGC performance

The industry's most established and reliable performance GCxGC technology.

Capable of mass resolutions of up to 50,000 and acquisition rates up to 200 spectra/second.

Methyl Undecanoate GCxGC 1 modulation

4800.650

4800.700

4800.750

4800.800

4800.850

4800.900

1st Time (s)2nd Time (s)

Peak Tailing < 1.5

EI Source

60 msec

For more information on EFP refer to technical brief 209-066-018.

HR-EI or HR-CI sources

Folded Flight Path® (FFP ®) provides high order TOF focusing which delivers high resolution sensitivity with two modes of operation (25 k and 50 k mass resolution)

System designed for very fast acquisition rates needed to define narrow second dimension peaks

KADAS – Patented acquisition system, which dramatically reduces noise, ensures mass accuracy, and reduces file size

New Encoded Frequent Pushing – Patented method of pulsing the Orthogonal Accelerator multiple times to increase duty cycle (x10)

For more information on EFP refer to technical brief 209-066-018.

The industry’s most established and reliable performance GC×GC technology.

Capable of mass resolutions of up to 50,000 and acquisition rates up to 200 spectra/second.

PEGASUS ® GC-HRT + 4D Hardware

Our integrated all-in-one ChromaTOF ® brand software controls all hardware elements, automatically calculates mass accuracies, and identifies 2nd dimension peaks. With innovative features and functions based on our

years of experience in GC×GC, the software is an exceptional tool to efficiently deliver maximum chemical information per sample.

Make GC×GC easier

• Take advantage of the structured nature of GC×GC chromatograms with the Classification feature

• Simply GC×GC® method development tool

• A new standard GC×GC column setup and methods, allows users to be successful out of the box

• Easily switch between one-dimensional and GC×GC modes of operation

• GC×GC parameter checking for standard application column set

• Enhanced data filtering tools (peak true filter)

• Two-dimensional chromatographic resolution calculator

Unknown Compound

HRD of GC × GC (both EI and CI)

1

1 2

3 4

Tentative Assignment

Spectral Match Top Hit

2Confidence

Grows

Retention time in both

1st and 2nd dimensions

3Identification

Compare to standards and use of

deconvoluted fragments

4

PEGASUS ® GC-HRT + 4D Software

Identify more with confidence

The complementary nature of HR-CI is shown in this example. Pseudo-molecular ions (loss of H atom) confirm the presence of the identification of pentadecane from the CI mass spectrum.

Identify Components with ConfidenceHigh Resolution Deconvolution® (HRD ®) Example: Diesel GC×GC

The combination of HRD and mass accuracy gives users exceptional confidence in identification.

Identification close, but not precise.

C H O (192)11 12 3

O

O

O

Calculated MassMass Accuracy

(ppm)

C H O11 12 3 192.0782 192.0781 0.29

C H O10 12 2 164.0833 164.0832 0.53

C H O8 7 2 135.044 135.0441 -0.75

C H O7 6 2 122.0363 122.0362 0.47

C H O7 5 105.0335 105.0335 0.38

C H6 5 77.03855 77.03858 -0.4

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500

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1.600

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1.800

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2.000

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2.200

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2.400

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2.600

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500

1000

1500

2000

2500

3000

1st Time (s)

2nd Time (s)

XIC(45.033±0.005) XIC(93.069±0.005) XIC(105.070±0.005)XIC(205.158±0.005) XIC(131.085±0.005)

Unknown identification is made easy with the PEGASUS ® GC-HRT + 4D. Example: Perfume Investigation

Identification close, but not precise

Odor Type: Spicy

Odor Type: Floral

Make Your Unknowns Known

The process of improving identifications is simplified by the GC-HRT+ 4D. In this case, there is a moderate identification from our Pegasus 4D (top) of an analyte with very different odour properties.

The Pegasus GC-HRT+ 4D (bottom) has improved the identification of this component to a more odour appropriate hit, as well as being more consistent with the data.

One modulation period is depicted below, fast spectra acquisition rates are vital to deconvolute complex regions of the chromatogram. Fast modulation is needed to maintain the first dimension of chromatography.

If a peak in the second dimension chromatogram is 60 msec wide, it requires 200 Hz to sample appropriately for effective deconvolution.

Find more peaks

Our Commitment to Quality and Service

LECO instruments are noted for superior precision, speed, and ease-of-use. We are an international company with over 25 subsidiaries worldwide. Our global network of sales/support is dedicated to customer service and satisfaction, and our commitment to quality is further underscored with ISO-9001:2015 certification. We conform to CE quality and safety specifications, fully testing our instruments at our on-site Compliance Testing Centre.

Find more peaks than ever before using high resolution GC×GC. These insets of a diesel sample show the

difference in data collected using one-dimensional (left) and two-dimensional chromatography (right).

Find More Peaks Using GC×GC and Discover:What Else Is In Your Sample

Be More Confident In Your AssignmentsBetter understand the chemistry of your sample by finding out what else is in your sample.

ISO-9001:2015 Q-994 | LECO is a registered trademark of LECO Corporation. LECO, Pegasus, ChromaTOF, Folded Flight Path, FFP, High Resolution Deconvolution, HRD, KADAS, Encoded Frequent Pushing and EFP are registered trademarks of LECO Corporation. Microsoft, Windows are registered trademarks of Microsoft Corporation.

PEGASUS ® GC-HRT+ 4D

Deconvolution | GC×GC

Productivity | Reproducibility

Accurate Mass | identification

Form No. 209-245-EUW 7/19-REV2 © 2019 LECO Corporation

LECO Corporation3000 Lakeview Avenue | St. Joseph, MI 49085Phone: 269 – 985 – 5496info@leco.com | www.leco.com

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