pectinase prelab please copy the following definitions: (on handout) chemical reaction: when atoms...
TRANSCRIPT
![Page 1: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/1.jpg)
Pectinase Prelab
Please copy the following definitions: (on handout)
Chemical reaction: when atoms are rearranged in a molecule or molecules.
Enzyme: a protein that speeds up a chemical reaction
Substrate: the substance an enzyme changes Catalyst: anything that speeds up a reaction
![Page 2: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/2.jpg)
The real chemical name for pectin is polygalacturose. What is the name of the monosaccharide it is made of?
![Page 3: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/3.jpg)
“Ga-lac-tur-ose”
It’s the monosaccharide subunit of poly-
galactarose.
Science words always make
sense, because they’re designed
to be used by speakers of
every language
Check Yourself:
There’s lots of different kinds of “sugars”out there.
Galacturose has 6 carbon atoms – how many oxygen atoms must it have?
![Page 4: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/4.jpg)
Here’s a small part of the long chain…
This is the bond we’ll be breaking in the lab!!!
![Page 5: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/5.jpg)
Pectinase is the
protein we’ll be using in the lab.
![Page 6: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/6.jpg)
ENZYME
SUBSTRATE
![Page 7: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/7.jpg)
Pectinase attaches to pectin at the active site…
![Page 8: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/8.jpg)
…And then breaks a bond in the polysaccharide!
![Page 9: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/9.jpg)
Some proteins (like Pectinase) are enzymes. Different enzymes speed up
all different kinds of reactions in organisms!
![Page 10: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/10.jpg)
SUBSTRATE
Enzyme
Enzymes are very selective about whichsubstrates they bind.In other words,
They’re Specific.
![Page 11: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/11.jpg)
Apples have lots of pectin
![Page 12: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/12.jpg)
We will be measuring the effect of pectinase by measuring how fast
liquid from applesauce drips through a filter.
What effect will chopping pectin into little pieces have on the amount of juice you collect?
![Page 13: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/13.jpg)
Homework:
Write a hypothesis on page 2 of the lab report based on what you know.
It should answer the question “What will pectinase do to pectin in applesauce?”
Hypothesize!
![Page 14: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/14.jpg)
Predict
• Remember what a prediction is: An if… then statement that will test your hypothesis using data from the experiment.
• If… [hypothesis] then [data you expect]
• Write a prediction on your lab report
![Page 15: Pectinase Prelab Please copy the following definitions: (on handout) Chemical reaction: when atoms are rearranged in a molecule or molecules. Enzyme: a](https://reader036.vdocuments.mx/reader036/viewer/2022062309/5697bf7a1a28abf838c82a55/html5/thumbnails/15.jpg)
Background
• With the time that remains, begin writing your background section on page 1 of the lab report. Use the bullet points listed to guide your response.