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PATTERNS OF CYTOCHROME OXYDASE ACTIVITY IN THE FRONTO-OPERCULAR CORTEX OF MACAQUE MONKEYS.

SHIN-ICHI ITO and HISASHI OGAWA. Dept. Physiol., Kumamoto Univ. Med. Sch., Honjo 2-2-1, Kumamoto 860

The laminar pattern of the cytochrome oxydase (CO) activity was studied in the fronto-opercular cortex (Fop) of three Japanese macaques following the Wong-Riley method. Several areas were demarcated with their characteristic pattern of stripes of enzymatic activity across the cortical layers, which were largely in accordance with the cytoarchitectonic areas with some exceptions. At the area 3b around the inferior precentral sulcus, five stripes were evident. The external stripe was dark and thin. Below the pale zone of stripe 2 lay a thick, intensively stained band, stripe 3. The most internal stripe (fifth) was less dark than the third. Between stripes 3 and 5 a lightly stained sharp band, stripe 4 stood out. Both stripes 3 and 4 made this area quite identifiable. Lateral to area 3b, and in a large part of the buried Fop lay the area 1-2, where stripe 3 became thinner and less dark and stripe 4 and 5 merged into a unity, diminishing the pale line of stripe 4. The lack of this stripe was characteristic in most cortices of buried Fop. Medial to the area 1-2, around the superior limitting sulcus (sls), lay the taste nerve projection area (G). In the medial part of area G and the adjacent so-called insula, stripe 3 again became quite dark and thick. Rostrally, this thick, dark band extended over the rostral end of the areas 1-2, where it became less dark and thinner, and merged into OFO-Prco which occupied Fop around the rostral limits of sls. Five stripes were evident there, all of which were less sharply demarcated because of moderate staining. Caudally, stripe 3 of this area seemed continuous to that of second somatic sensory area (SII) whose stripe 3 was similarly dark and thick. This intensively stained band extended from there in Fop to a large part of the buried parietoperculum, along the course of SII. Studies on the visual cortex have identified the CO-rich layer as termination zone of the specific thalamocortical projection. Therefore, it is suggested that the area around the sls, including area G, receives heavy projections from the thalamic relay nuclei, as the case of area 3b and SII.

TELENCEPHALON SPECIFIC ANTIGEN - AN IMMUNOH]STOCHEMICAL ANALYSYS USING A MONOCLONAL ANTIBODY

KENSAKU MORI a), SHINOBU C. FUJITA b), YASUYOSHI WATANABEC~ d), KUNIH]KO OBATA b) and OSAMU HAYAISHI a), Departments of a)Physiology and ~DJPharmacology, Gunma University School of Medicine, Maebashi, Gunma 371, UJDepartment o[1 Medical Chemistry, Osaka Medical College, Takatsuki, Osaka 569, and 'Hayaishi Bfoinformation Transfer Project, Research Development Corporation of" Japan, c/o Osaka Medical College, TakatsukJ, Osaka'569, Japan

Monoclonal antibodies were produced using a mouse immunized with fractions

rich in the dendrodendritic synaptosomes of the rabbit olfactory bulb.

]mmunohistochemical examination of the rabbit nervous system demonstrated that

one of the HAbs (MAb 271A6) strongly labeled the grey matter of" all the

telencephalic regions such as the neocortex, the piriform cortex, the

hJppocampus, the striatum, and the amygdaloid nucleus~ in addition to the

external plexiform and granule cell layers of the olfactory bulb. However, MAb

271A6 did not label non-telencepha]ic regions of the central nervous system. No

immunoreactivity was observed in the peripheral nervous system so far examined.

Thus, the antigen 271A6 (the molecule(s) Recognized by MAb 271A6) seems to be

expressed specifically in all Regions of the telencephalon. These results

indicate a molecular specificity of the most rostral brain segment, the

telencepha]on. MAb 27]A6 may provide a means fOR studying the molecular basis

of' segmental organization and/or segment specific function of the [)rain.