oxygen absorption in hemoglobin and brain activity measurement · motivation za need for...
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Oxygen Absorption in Hemoglobin and Brain Activity Measurement
Quantitation of Oxygen ConcentrationShakera Guess
Advisor: Dr. Britton Chance Ph. D, Sc. D, M.D
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Motivation
Current methods of lie detection– Polygraph
Breathing rate, pulse, blood pressure, perspirationAnxiety Detector
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Motivation
Current methods of lie detection– fMRI
Brain mappingCurrently most favorableImmobilizationExpensive
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Motivation
A need for non-invasively measuring cognitive function
Current methods of measuring brain function include positron emission tomography (PET imaging) and electroencephalography (EEG).
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Background
•Near Infrared Spectroscopy (NIRS)•Many benefits
•Used in a variety of procedures
•700-900 nmLCC HbOHbOHbHbeGII )(
022αα +−=
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– Absorption in body contains information about hemoglobin level changes
– Thinking = BV level changes
– Biological tissue transparent 700-900 nm
– NIRS can predict certain thought processes
Background
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Project Goals
Different methods of quantitationNIRS and Cognosensor
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Oxygen Absorption in Yeast
Oxygen Absorption Measurement in Yeast UsingOxygen Electrode
0
50
100
150
200
1 2 3 4 5 6 7
Time (min)
Absorption
Yeast
Water
•Results of experiment performed with Clarke Oxygen Electrode, yeast, and water.
•Electrode used to connect with the non-metallic part of a circuit
using equation
O2 + H2O + 2e- → H2O2 + 2OH-
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Oxygen Absorption in Hemoglobin
Oxygen Electrode Data 7/19
0
50
100
150
200
250
0 7
14
21
28
35
42
49
56
63
70
77
Time
Intensity
Intensity•Results of oxygen absorption measurement in hemoglobin performed with Clarke Oxygen Electrode.
Oxygen (Electrode) and Hemoglobin (Brain Probe)Data Merged
-300
-200
-100
0
100
2000
27.7
50.5
53.3
61.1
88.8
117
144
Time
Intensity/Blood
Volume
Oxygen
Hemoglobin (x5)
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Further Uses
Early breast cancer detection– Women under 40– High risk– Underserved population
Right Left Right Left
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Further Uses
Lie detection– Many ground-
breaking studies– Changes in blood
oxygenation in cortex– Cognitive components
of deception and emotional response
– NIRS used to differentiate
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Conclusions
Near Infra-Red Spectroscopy (NIRS) used to measure the change of blood volumes in the brainSimple and cheap handheld device used to non-invasively measure cognitive functionMany uses including lie detection and early breast cancer detection
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Acknowledgements
SUNFEST ParticipantsDr. Britton ChanceDr. Van der Spiegel– His office, DeloresSpecial thanks to the
National Science Foundation and the University of Pennsylvania.
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Thank You!
Shakera Guess