oxford electron microscope proposal · web viewfor the review process, the application must include...

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Application for use of Cryo Electron Microscopy (Titan Krios) Completed application should be emailed to [email protected]. Pre-screening of grids on Arctica is required before data collection on Krios. Project Title Principal Investigator Details: Name Department/ Institution Email & Contact No. Cost Centre chargeable Start date of grant: End date of grant: Details of research staff using Titan Krios: Name Department/ Institution Email & Contact No. Designation Postgraduate Student PO/RA Postdoctoral Fellows Others _______________________ Project’s Detail: Brief Sample Information (Molecular weight, multimerisation state, type of grids) * Please submit sample declaration form to the EM manager Please indicate the amount of time required. Time required: _______ days /year Safety/Risk Assessment I have submitted OHSE’s online risk assessment for work conducted in CryoEM facility and transportation of samples to CryoEM facility. I have gone through and understood the RA and safety work procedures prepared by the EM lab and have signed the acknowledgment form.

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Page 1: Oxford Electron Microscope Proposal · Web viewFor the review process, the application must include images and evidence that suitable cryo-EM samples have already been produced (e.g

Application for use of Cryo Electron Microscopy (Titan Krios)

Completed application should be emailed to [email protected]. Pre-screening of grids on Arctica is required before data collection on Krios.

Project Title

Principal Investigator Details:Name

Department/Institution

Email & Contact No.

Cost Centre chargeable Start date of grant: End date of grant:

Details of research staff using Titan Krios:Name

Department/Institution

Email & Contact No.

Designation ☐ Postgraduate Student ☐ PO/RA ☐ Postdoctoral Fellows☐ Others _______________________

Project’s Detail:Brief Sample Information (Molecular weight, multimerisation state, type of grids)

* Please submit sample declaration form to the EM manager

Please indicate the amount of time required. Time required: _______ days /year

Safety/Risk Assessment☐ I have submitted OHSE’s online risk assessment for work conducted in CryoEM facility and transportation of samples to CryoEM facility.☐ I have gone through and understood the RA and safety work procedures prepared by the EM lab and have signed the acknowledgment form.

Declaration:To agree by the rules and regulations in using CryoEM facility.I declare that I have the necessary funding and to acknowledge NISB in publications. I understand that any violations could result in cessation of the use of facilities in the future.

Signature of Principal Investigator:

Date:

Page 2: Oxford Electron Microscope Proposal · Web viewFor the review process, the application must include images and evidence that suitable cryo-EM samples have already been produced (e.g

Brief Project Details (1000 words max ) & key objectives (300 words max). For the review process, the application must include images and evidence that suitable cryo-EM samples have already been produced (e.g. field views of grids, images of 2D classes calculated from the cryo-EM sample under investigation which demonstrate secondary structure). Failure to provide these data will result in the application being returned. All proposals are awarded time based on scientific merit. Users are required to process data using WARP software during collecting data and to save a screenshot of the processing results.

Key Objectives:

Project Details:

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