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Choosing the best secondary antibody for your research

IHC ELISA FCWB EMSAIHC

Important selection steps:1. Match the host of the primary antibody2. Match the class/subclass of the primary antibody 3. Select the correct reporter for your application

Rockland is the trusted source for secondary antibodies for over 50 years.

Criteria Description

HostSecondary antibody should specifically detect the species that produced the primary antibody.

Polyclonal or Monoclonal

Polyclonal antibodies are sufficient for most needs. Monoclonal antibodies are more difficult to produce but are highly specific and yield more consistent results over time.

Conjugates by Application

For Western blot and ELISA, an enzyme conjugated secondary is most likely the best choice. Good examples are Peroxidase or Alkaline phosphatase. For IP experiments, a special product that does not detect the precipitating antibody is essential for best results. Rockland’s TrueBlot® products are useful for the accurate detection of secondary antibodies used for IP followed by western blot.

Other Specificities

Subclass, Fab or F(ab’)2 fragments, reduced cross reaction to other species.

To Learn More, Visit: www.rockland-inc.com/reliable-results

For our full list of over 2,500 secondary

antibodies, visit www.rockland-inc.com

B

Product Name Catalog # Size ApplicationAnti-ARMENIAN HAMSTER IgG (H&L) (GOAT) Peroxidase Conjugated 619-103-002 1 mg ELISA, WB, IHC

Anti-CHICKEN IgG (H&L) (GOAT) Peroxidase Conjugated 603-103-002 2 mg ELISA, WB, IHC

Anti-CHICKEN IgG (H&L) (RABBIT) Peroxidase Conjugated 603-4302 1.5 mg ELISA, WB, IHC

Anti-GOAT IgG (H&L) (DONKEY) Peroxidase Conjugated 605-703-002 2 mg ELISA, WB, IHC

Anti-GOAT IgG (H&L) (RABBIT) Peroxidase Conjugated 605-4302 2 mg ELISA, WB, IHC

Anti-HUMAN IgG (H&L) (CHICKEN) Peroxidase Conjugated 609-9302 1 mg ELISA, WB, IHC

Anti-HUMAN IgG (H&L) (RABBIT) Peroxidase Conjugated 609-4302 2 mg ELISA, WB, IHC

Anti-MONKEY IgA (alpha chain) (GOAT) Peroxidase Conjugated 617-103-006 1 mg ELISA, WB, IHC

Anti-MONKEY IgM (mu chain) (GOAT) Peroxidase Conjugated 617-103-007 1 mg ELISA, WB, IHC

Anti-MOUSE IgG (H&L) (GOAT) Peroxidase Conjugated 610-1302 2 mg ELISA, WB, IHC

Anti-MOUSE IgG1 (Gamma 1 chain) (RABBIT) Peroxidase Conjugated 610-4340 1 mg ELISA, WB, IHC

Anti-RABBIT IgG (H&L) (DONKEY) Peroxidase Conjugated 611-7302 2 mg ELISA, WB, IHC

Anti-RABBIT IgG (H&L) (GOAT) Peroxidase Conjugated 611-1302 2 mg ELISA, WB, IHC

Anti-CHICKEN IgG (H&L) (RABBIT) 603-101-002 2 mg ELISA, WB, IHC

Anti-HUMAN IgG F(c) (GOAT) 609-1103 5 mg ELISA, WB, IHC

Anti-HUMAN IgG F(c) (GOAT) (Min X MOUSE Serum Proteins) 609-101-017 1 mg ELISA, WB, IHC

Anti-HUMAN IgM (Fc5u) (RABBIT) 609-4131 2 mg ELISA, WB, IHC

Anti-MONKEY IgG (gamma chain) (GOAT) 617-101-012 1 mg ELISA, WB, IHC

Anti-MONKEY IgG IgA IgM (H&L) (GOAT) 617-101-130 1 mg ELISA, WB, IHC

Anti-MOUSE IgG (H&L) (GOAT) 610-1102 2 mg ELISA, FLISA, WB, IHC, IF

Anti-MOUSE IgG F(c) (GOAT) 610-1103 2 mg ELISA, WB, IHC

Anti-MOUSE IgG2b (Gamma 2b chain) (RABBIT) 610-4142 1 mg ELISA, WB, IHC

Anti-RABBIT IgG (H&L) (GOAT) 611-1102 2 mg ELISA, WB, IHC, IF

Horseradish Peroxidase Conjugated

Product Name Catalog # Size ApplicationAnti-GOAT IgG (H&L) (DONKEY) DyLight™ 680 Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) 605-744-125 100 µg FLISA, WB, IF

Anti-HUMAN IgG (H&L) (GOAT) DyLight™ 649 Conjugated (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins) 609-143-123 100 µg FLISA, WB, IF

Anti-MOUSE IgG (H&L) (DONKEY) DyLight™ 680 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) 610-744-124 100 µg FLISA, WB, IF

Anti-MOUSE IgG (H&L) (DONKEY) DyLight™ 800 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) 610-745-124 100 µg FLISA, WB, IF

Anti-MOUSE IgG (H&L) (GOAT) DyLight™ 488 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) 610-141-121 100 µg FLISA, WB, IF

Anti-MOUSE IgG F(c) (GOAT) DyLight™ 488 Conjugated 610-141-003 100 µg FLISA, WB, IF

Anti-MOUSE IgG F(c) (GOAT) DyLight™ 680 Conjugated 610-144-003 100 µg FLISA, WB, IF

Anti-RABBIT IgG (H&L) (GOAT) DyLight™ 549 Conjugated 611-142-002 100 µg FLISA, WB, IF

Anti-RABBIT IgG (H&L) (GOAT) DyLight™ 549 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) 611-142-122 100 µg FLISA, WB, IF

Anti-RABBIT IgG (H&L) (GOAT) DyLight™ 680 Conjugated 611-144-002 100 µg FLISA, WB, IF

Anti-RABBIT IgG (H&L) (GOAT) DyLight™ 800 Conjugated 611-145-002 100 µg FLISA, WB, IF

Anti-RAT IgG (H&L) (GOAT) DyLight™ 549 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) 612-142-120 100 µg FLISA, WB, IF

Anti-CHICKEN IgG (H&L) (RABBIT) Fluorescein Conjugated 603-4202 1.5 mg FLISA, IF

Anti-DOG IgG F(c) (GOAT) Fluorescein Conjugated 604-1203 2 mg FLISA, IF

Anti-FERRET IgG IgA IgM (H&L) (GOAT) Fluorescein Conjugated 618-102-130 1 mg FLISA, IF

Anti-GOAT IgG (H&L) (DONKEY) Fluorescein Conjugated 605-702-002 2 mg FLISA, IF

Anti-HUMAN IgG (gamma chain) (RABBIT) Fluorescein Conjugated 609-4212 1 mg FLISA, IF

Anti-MONKEY IgM (mu chain) (GOAT) Fluorescein Conjugated 617-102-007 1 mg FLISA, IF

Anti-MOUSE IgG (H&L) (GOAT) Fluorescein Conjugated 610-1202 2 mg FLISA, IF

Anti-MOUSE IgG (H&L) (GOAT) Fluorescein Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) 610-102-121 1 mg FLISA, IF

Anti-MOUSE IgG2a (RABBIT) Fluorescein Conjugated 610-4241 1 mg FLISA, IF

Anti-RABBIT IgG (H&L) (GOAT) Fluorescein Conjugated 611-1202 2 mg FLISA, IF

Anti-RABBIT IgG (H&L) (GOAT) Fluorescein Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) 611-102-122 1 mg FLISA, IF

Anti-RAT IgG (H&L) (GOAT) Fluorescein Conjugated 612-1202 2 mg IF

Anti-RAT IgG F(c) (GOAT) Fluorescein Conjugated 612-1203 2 mg FLISA, IF

Anti-RAT-IgG (H&L) (RABBIT) Fluorescein Conjugated 612-4202 2 mg FLISA, IF

F(ab')2 Anti-HUMAN κ (kappa chain) (GOAT) Fluorescein Conjugated 709-1210 1 mg FLISA, IF

F(ab')2 Anti-HUMAN λ (lambda chain) (GOAT) Fluorescein Conjugated 709-1211 1 mg FLISA, IF

Fab Anti-RABBIT IgG (H&L) (GOAT) Fluorescein Conjugated 811-1202 1 mg FLISA, IF

F(ab')2 Anti-RABBIT IgG (GOAT) (Min X Human Serum Proteins) 611-1124 5 mg ELISA, WB, IHC

F(ab')2 Anti-HUMAN IgG (H&L) (GOAT) Min X Bv Hs Ms & Rt Proteins 709-1116 1 mg ELISA, WB, IHC

F(ab')2 Anti-HUMAN IgG F(c) (GOAT) Min X Bv Hs Ms & Rt Proteins 709-1117 1 mg ELISA, WB, IHCF(ab')2 Anti-MOUSE IgG (H&L) (DONKEY) Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins 710-708-124 500 µg IF

F(ab')2 Anti-MOUSE IgG F(c) (GOAT) Min X Bv Hs & Hu Proteins 710-1122 1 mg ELISA, WB, IHC

F(ab')2 Anti-MOUSE IgM (mu chain) (GOAT) 710-1107 1 mg ELISA, WB, IHC

Fab Anti-CHICKEN IgG (H&L) (GOAT) Biotin Conjugated 803-1602 1 mg ELISA, WB, IHC

Fab Anti-CHICKEN IgG (H&L) (GOAT) Peroxidase Conjugated 803-1302 1 mg ELISA, WB, IHC

Fab Anti-GOAT IgG (H&L) (DONKEY) Biotin Conjugated 805-7602 1 mg ELISA, WB, IHC

Fab Anti-GOAT IgG (H&L) (DONKEY) Peroxidase Conjugated 805-7302 1 mg ELISA, WB, IHC

Fab Anti-GS HAMSTER IgG (H&L) (GOAT) Peroxidase Conjugated 807-1302 1 mg ELISA, WB, IHC

Fab Anti-GUINEA PIG IgG (H&L) (GOAT) Fluorescein Conjugated 806-1202 1 mg FLISA, IF

Fab Anti-HUMAN IgG (H&L) (GOAT) 809-1102 1 mg ELISA, WB, IHC

Fab Anti-MOUSE IgG (H&L) (GOAT) 810-1102 1 mg ELISA, WB, IHC

Fab Anti-RABBIT IgG (H&L) (DONKEY) Fluorescein Conjugated 811-7202 1 mg FLISA, IF

Fluorescein Conjugated

Unconjugated

Antibody Fragments

DyLight™ Conjugated