oris™ cell-based assays - amsbio cell-based assays cancer metastases cell proliferation drug...

Accelerate Your Cell Motility Research

ORIS™ CELL-BASED ASSAYSORIS™ CELL-BASED ASSAYSORIS™ CELL-BASED ASSAYS

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Imaging (HCI) cancer metastases cell proliferation drug discovery siRNA screening angiogenesis





































CELL MIGRATION

CELL MOTILITY

CELL INVASION

www.amsbio.com [email protected]

Visually Assess Cell Movement and Morphology

Oris™ and Oris™ Pro Cell Migration AssaysOris™ and Oris™ Pro Cell Migration AssaysOris™ and Oris™ Pro Cell Migration Assays

Product OverviewOris™ assays employ exclusion zone technology to form centrally located cell-free zones in the centers of cell monolayers. The zones are created by the use of patented silicone stoppers in the Oris™ format and by a proprietary patent-pending biocompatible gel (BCG) in the Oris™ Pro assays.

Oris™ stopper strip Oris™ Pro BCG

Assay Schematic

Product Highlights• Reproducible results

Achieve low well-to-well CVs

• Robust assayObtain Z’ factors suitable for compound screening

• Versatile readoutUse microscopes, high content imagers and microplate readers

• More physiological relevance Integrity of Extracellular Matrix (ECM) remains intact

• Increased productivity Treat cells with multiple dyes or antibodies to reveal more information per assay well

Product VersatilityOris™ (stopper-based) Assays in 96-well plates

Plate coatingsTC, Collagen I, Fibronectin and TriCoat (32 wells of each coating)

Plate readinessStoppers pre-populated or on the side to permit coating with your choice of ECM

Plates for bench studies96 stoppers that can be populated in strips of 4 across 4 plates for users performing fewer assays

Oris™ Pro (BCG-based) Assays in 96-well and 384-well plates Plate coatings

TC and Collagen I

Representative Data

0.0001 0.001 0.01 0.1 1 10-50

0

50

100

150

OrisTM

OrisTM Pro

OrisTM Pro 384

Cytochalasin D(μM)

Are

a C

losu

re(%

)

Cytochalasin DIC50

0.123 µM0.159 µM0.121 µM

Z’ Factor

0.570.610.71

HUVEC cells were seeded in the 96-well Oris™, 96-well Oris™ Pro and 384-well Oris™ Pro TC plates. Following attachment, stoppers were pulled and the BCG dissolved. Cells were treated with the actin polymerization inhibitor, Cytochalasin D. After 18 hours, cells were stained with TRITC-phalloidin and images were captured on an inverted microscope, n = 4 wells/condition.

Additional Features of Oris™ Pro Assays• High-throughput

Providing 96 and 384 assay wells per plate

• AutomatableUnlimited access to wells by liquid handling instruments

• Less handlingBCG self-dissolves to reveal detection zone (no need to remove stoppers)

• Fewer stepsNo wash steps or additional reagents required

Certain uses of these products may be covered by U.S. Pat. 7,018,838 and 7,842,499 and applications 10/597,118, 11/342,413, and 12/195,007.


Mimic Physiological Conditions

Product OverviewOris™ invasion assays employ exclusion zone technology to form centrally located cell-free zones in the centers of cell monolayers. The zones are created on top of a layer of extracellular matrix (ECM) by the use of patented silicone stoppers in the Oris™ format and by a proprietary patent-pending biocompatible gel (BCG) in the Oris™ Pro assays. A second layer of ECM applied to the well envelopes the cells. In this assay, in vitro movement of cells through the 3D ECM mimics an in vivo environment.

Assay Schematic

Product Highlights• Reproducible results

Achieve low well-to-well CVs

• High content amenableCollect image-based multi-parametric data

• More physiological relevanceRealize a more native environment in extracellular matrices

• Acquire 3-dimensional dataQuantify invasion in the x, y and z-planes

Product VersatilityOris™ (stopper-based) Assays in 96-well plates

Plate coatings Collagen I and other ECM overlays

Oris™ Pro (BCG-based) Assays in 96-well plates Plate coatings

Collagen I

Representative Data

0 20 40 60 80 100 1200

500

1000

1500

0 µM0.031 µM0.125 µM0.5 µM2 µM

Z-plane ( m)

Num

ber o

f Cel

ls

µ

Cytochalasin D

120 µm0 µm 40 µm 80 µm

1 µM

0 µ MCy

toch

alas

in D

Z-plane

HT-1080 cells were seeded in 96-well Oris™ Pro Collagen I invasion plates and overlaid with 3 mg/mL Collagen I. Cells were treated with the actin polymerization inhibitor, Cytochalasin D. Following a 72 hour incubation, nuclei were counted in the detection zone of each z-plane from 0 to 120 µm using Molecular Device’s Isocyte with AcuityXpressTM software.

Multiplexed Staining for High Content Analysis

HT-1080 Cells Invading into BME

Co-localized F-actin and Cortactin

Expression of MMP-9 protease

Expression of Cathepsin B


Your Search for Cell Motility Assays Ends Here

ORIS™ CELL-BASED ASSAYSORIS™ CELL-BASED ASSAYSORIS™ CELL-BASED ASSAYS

Getting Started with Oris™ Assays


oris™ cell-based assays - amsbio cell-based assays cancer metastases cell proliferation drug...

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