Óragének (és a melatonin)
DESCRIPTION
Óragének (és a melatonin). Rékási Zoltán PTE ÁOK Anatómiai Intézet. birds. mammals. Melatonin elválasztás explantált patkány tobozmirigyből normál in vitro megvilágítási ciklusban. Melatonin elválasztás explantált csirke tobozmirigyből - PowerPoint PPT PresentationTRANSCRIPT
Óragének (és a melatonin) Óragének (és a melatonin)
Rékási Zoltán Rékási Zoltán
PTE PTE ÁOK ÁOK Anatómiai IntézetAnatómiai Intézet
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birds mammals
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5 % CO2 +95 % AIR
PERISTALTICPUMP
FRACTION COLLECTOR
FOR SAMPLEDILUTION
37 Co
WATERBATH
AIRW ITHDRAWAL SAMPLE
MEDIUMSAMPLE
CE
LL
S +
SE
PH
AD
EX
WATERJACKET
C s.V .
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Melatonin elválasztás explantált patkány tobozmirigyből normál in vitro megvilágítási ciklusban
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0
10
20
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18 0 6 12 18 0 6 12 18 0 6 12 18 0 6hours
Melatonin elválasztás explantált csirke tobozmirigybőlnormál in vitro megvilágítási ciklusban
Melatonin elválasztás explantált csirke tobozmirigybőlnormál in vitro megvilágítási ciklusban
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CH2CH2 NH2HO
NH
NH CH3
OCCH2CH2HO
NH
NH CH3
OCCH2CH2O
NH
CH3
SEROTONIN
N-ACETYLSEROTONIN
MELATONIN
N-acetyltransferase (AA-NAT)
hydroxyindol-o-methyltransferase (HIOMT) SAdM
AcCoA
MELATONIN SYNTHESIS
LIGHT
MELATONIN RELEASE
SIGNAL TRANSDUCTION
Adrenergic and VPAC-RECEPTORS
NORADRENERGIC and PEPTIDERGIC PATHWAYS
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CH2CH2 NH2HO
NH
NH CH3
OCCH2CH2HO
NH
NH CH3
OCCH2CH2O
NH
CH3
SEROTONIN
N-ACETYLSEROTONIN
MELATONIN
N-acetyltransferase (AA-NAT)
hydroxyindol-o-methyltransferase (HIOMT) SAdM
AcCoA
PHOTORECEPTORS
CLOCK (PACEMAKER) Clock-genes
MELATONIN SYNTHESIS
LIGHT
MELATONIN RELEASE
? ?
?
SIGNAL TRANSDUCTION
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period timeless
TIMPER
Clock
CLOCK BMAL1
Bmal1
Activatio
nInh
ibit
ion
time
gen
e ex
pre
ssio
n
Four-gene core transcription-translation loopFour-gene core transcription-translation loop
DELAY
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Clock-genes in Drosophila and mammals Clock-genes in Drosophila and mammals PERIOD1
PERIOD2
PERIOD3
NLS bHLH/PAS
PERIOD
TIMELESS
NLS NLS NLS
CLOCK
CYCLE BMAL1
Doubletime casein kinase I epsilon
Cryptochrome2
CryptochromeCryptochrome1
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Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken pineal gland
Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken pineal gland
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Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken retina
Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken retina
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Expression of retinal and pineal cAANAT mRNA in chicken housed
in constant darkness for 1 day or 1 week
Expression of retinal and pineal cAANAT mRNA in chicken housed
in constant darkness for 1 day or 1 week
retina pineal
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Expression of retinal and pineal cBmal1 mRNA in chicken housed
in constant darkness for 1 day or 1 week
Expression of retinal and pineal cBmal1 mRNA in chicken housed
in constant darkness for 1 day or 1 week
retina pineal
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Expression of retinal and pineal cAANAT mRNA in chicken housed in constant light for 1 day or 1 week
Expression of retinal and pineal cAANAT mRNA in chicken housed in constant light for 1 day or 1 week
retina pineal
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retina pineal
Expression of retinal and pineal cBmal1 mRNA in chicken housed
in constant light for 1 day or 1 week
Expression of retinal and pineal cBmal1 mRNA in chicken housed
in constant light for 1 day or 1 week
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Model of circadian clock-work within an individual retinal or pineal
photoreceptor cell
Model of circadian clock-work within an individual retinal or pineal
photoreceptor cell
cCry2
cCry1
cPer2
cPer3
cAANAT
Output/melatonin
Bmal1
Clock Resetting the clock by light
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