optical and atomic force microscopy for analysis of wet sediments from the canale dei navicelli life...
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LIFE 12 ENV IT 000652 Cleansed
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Two methods
Analysis with Optical Microscopy (shape, size, granulometry)
Analysis with scanning probe by AFM (Atomic Force Microscope)
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Optical Microscopy
Biological microscope with high-resolution (16 Mpixel) reflex on trinocular tube for digital imaging
Software:ImageJ (developed by the National Institute of Health): for quantitative analysis (real dimensions-pixel, particle counting)
Helicon Focus Pro: for image stacking (to increase the depth of field)
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AFM Microscopy
Nanosurf Easyscan 2 MicroscopeScanning head: 110*110 micronMaximum Z range: 22 micronMaximum resolution: 2048*2048 pixel
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Granulometry
The texture has great importance for the physical and biological properties of the soil
Classification (USDA):
oclay, diameter less than 2 micron osilt, diameter between 2 and 50 micronosand, between 50 micron and 2 mm.
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Sample 1
Sample 3
Total counting: 4002
Total counting: 2005
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Sample 5
Sample 7
Total counting: 2013
Total counting: 3442
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Sample 1
Cyclotella Meneghiniana (Moder. Cont.) Cyclotella Meneghiniana (MC)
Staurastrum anatinum (MC)
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Sample 1
Cyclotella Meneghiniana (MC) Melosira Varians (MC)
Navicula Rhyncotella (MC) Melosira Moniliformis (MC)
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Sample 1
Melosira Moniliformis (MC) Cyclotella Meneghiniana (MC)
Cyclotella Meneghiniana (MC) Bacillaria Paxillifer (MC to HC)
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Sample 1
AFM measurements of sedimentary rock composed by centric
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Sample 1
AFM: trace of diatom (Nitzschia Acicularis (MC to HC))
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Sample 3
Surirella Brebissonii (MC)
Nitschia Sublinearis (MC to HC) Gyrosigma Nodiferum (MC)
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Sample 3
AFM: trace of diatom (Phase contrast)
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Sample 3
AFM: trace of diatom
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Sample 3
AFM: trace of diatom
Zoom at 45 micron
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Sample 5
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Sample 7
Fragilaria Acus (SC)
Fragilaria Acus (SC)
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Sample 7
In some cases the AFM cantilever can displace a diatom:
Before:
After: