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Download Operating Manual Axio Observer Inverted microscope Axio Observer... Carl Zeiss Contents Axio Observer

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  • O p e r a t in

    g M

    a n u a l

    A x io

    O b s e r v e r

    In v e r t e d m

    ic r o s c o p e

  • Carl Zeiss Copyright / Trademarks Axio Observer

    2 B 46-0111 e 12/06

    Knowledge of this manual is required to operate the instrument. You should therefore familiarize yourself with the contents of this manual and pay special attention to instructions concerning the safe operation of the instrument.

    Technical specifications are subject to change; this operating manual will not be updated.

    © Unless expressly authorized, dissemination or duplication of this document and commercial exploitation or communication of its contents are not permitted. Persons in contravention of this copyright are liable to pay compensation for damages.

    All rights reserved in the events of granting of patents or registration as a utility patent.

    Any company and product names used in this manual may be trademarks or registered trademarks. Third party products are cited for information purposes only and this does not represent approval or recommendation of these products.

    Carl Zeiss MicroImaging GmbH accepts no liability for the performance or use of such products.

    Published by: Carl Zeiss MicroImaging GmbH P.O.B. 4041, 37030 Göttingen, Germany Phone: +49 (0) 551 5060 660 Fax: +49 (0) 551 5060 464 E-mail: micro@zeiss.de www.zeiss.de

    Manual number: B 46-0111 e

    Date of publication: Version 5 – 12/01/2006

  • Axio Observer Contents Carl Zeiss

    B 46-0111 e 12/06 3

    CONTENTS

    Page

    1 INTRODUCTION ..................................................................................................................8 1.1 Safety notes ..........................................................................................................................8 1.2 Notes on warranty ..............................................................................................................12 1.3 Stand types (overview) ........................................................................................................13

    2 DESCRIPTION ....................................................................................................................14 2.1 Designation and intended use .............................................................................................14 2.2 Description and main features.............................................................................................15 2.3 Equipment and compatibility table ......................................................................................16 2.4 System overview .................................................................................................................19 2.5 Objectives ...........................................................................................................................23 2.6 Eyepieces ............................................................................................................................25 2.7 Condensers.........................................................................................................................25 2.8 Specimen stages .................................................................................................................26 2.9 Binocular tubes ...................................................................................................................26 2.10 Technical specifications .......................................................................................................27

    3 SETTING UP THE INSTRUMENT ........................................................................................29 3.1 Unpacking and installing the microscope.............................................................................29 3.2 Attaching the binocular (photo) tube ..................................................................................30 3.2.1 Inserting the eyepieces and centering telescope...................................................................30 3.2.2 Inserting the eyepiece reticle ...............................................................................................31 3.3 Fitting the transmitted light illuminator................................................................................32 3.3.1 Fitting the 100 W transmitted light illuminator carrier..........................................................32 3.3.2 Fitting the 35 W transmitted light illuminator carrier............................................................32 3.4 Screwing in the objectives ...................................................................................................33 3.5 Fitting the microscope stages ..............................................................................................34 3.5.1 Fitting the mechanical stage 130x85 R/L and mounting frame K for the mechanical stage ...34 3.5.2 Fitting a scanning stage.......................................................................................................35 3.5.3 Attaching the specimen stage 250x230 , object guide and mounting frame M for the

    object guide........................................................................................................................36 3.5.4 Fitting the heating stage .....................................................................................................37 3.5.5 Fitting the gliding stage Z....................................................................................................38 3.6 Attaching the condensers....................................................................................................38 3.6.1 Condensers for the Axio Observer .......................................................................................38 3.6.2 Condensers from the Axio Imager range .............................................................................39 3.6.3 Changing the DIC prism in the condenser turret..................................................................40 3.7 Reflector turret....................................................................................................................41 3.7.1 Fitting the reflector turret....................................................................................................41 3.7.2 Loading the reflector turret .................................................................................................41 3.7.3 Changing the filter set in the FL P&C reflector module.........................................................42 3.7.4 Changing the beam splitter in the FL P&C reflector module .................................................43 3.8 Fitting the TFT display to the Z1 motorized stand.................................................................45

  • Carl Zeiss Contents Axio Observer

    4 B 46-0111 e 12/06

    3.9 Fitting the TFT display to the docking station.......................................................................45 3.10 Connecting the microscope to the mains power supply.......................................................46 3.11 Sockets on the rear of the Axio Observer.............................................................................47 3.11.1 A1 stand.............................................................................................................................47 3.11.2 D1 stand.............................................................................................................................47 3.11.3 Z1 stand..............................................................................................................................48 3.12 Switching the microscope and the power supply for the HBO 100 on and off .....................49 3.13 HAL 100 halogen illuminator...............................................................................................50 3.13.1 Attaching the HAL 100 halogen illuminator.........................................................................50 3.13.2 Adjusting the HAL 100 halogen illuminator .........................................................................51 3.13.3 Fitting and removing the diffuser for fine adjustment of the halogen illuminator .................52 3.13.4 Replacing the HAL 100 halogen bulb ..................................................................................53 3.14 HBO 100 illuminator ...........................................................................................................54 3.14.1 Inserting the HBO 103 W/2 mercury vapor short-arc bulb....................................................54 3.14.2 Attaching the HBO 100 illuminator .....................................................................................54 3.14.3 Adjusting the HBO 100 illuminator......................................................................................55 3.15 Fitting the Aqua Stop II .......................................................................................................56 3.16 Attaching the mounting adaptor for Laserport to the stand.................................................57

    4 OPERATION.......................................................................................................................58 4.1 Overview of components and controls ................................................................................58 4.1.1 Axio Observer.A1 (manual)..................................................................................................58 4.1.2 Axio Observer.D1 (coded, semi-motorized)................................

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