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Faculty of Agriculture and Environment, Agriculture University of Tirana, Albania

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Page 1: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

Faculty of Agriculture and Environment, Agriculture University of Tirana, Albania

Page 2: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

•Accredited laboratory in conformity with: SSH EN ISO / IEC 17025: 2006 Generalrequirements for the competence of testingand calibration laboratories.and calibration laboratories.

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• Academic staff and students support in scientific work regardenvironmental conservation, soil and water pollution assessment, landuse and management, Natural resource Management, soil erosion andconservation.•Impact of human activity on the environment.• Evaluation of environmental indicators.

Main objectives of LAME-UBT

• Evaluation of environmental indicators.• Analysis for clients regard soil, plant, water, etc.•Participation in proficiency tests (ISE).

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No Parameter Equipment Methods1 Cr AAS ISO2 Cu AAS ISO3 Pb AAS ISO4 Cd AAS ISO5 Ni AAS ISO6 Fe AAS ISO7 Co AAS ISO8 Mn AAS ISO9 K AAS ISO

10 Na AAS ISO11 Ca AAS ISO12 Mg AAS ISO

Zn AAS ISO

I. Soil analysis No Parameter Equipment Methods1 pH pH-meter ISO2 COD Spectrometry ISO3 BOD5 Ox top System SMFEWW/WTW4 N-NH4 Spectrometry Test kit/ISO5 Dissolved Oxygen Ox meter ISO6 N –total Spectrometry ISO7 N – NO3 Spectrometry Teskit/ SMFEWW8 N–NO2 Spectrometry Test kit/ISO9 P–PO4 Spectrometry Test kit/ISO

10 P- total Spectrometry ISO11 Phenol Alkalinity Volumetric ISO12 Cl Volumetric ISO13 Conductivity Conduct meter ISO

II. Water analysis

13 Zn AAS ISO14 N –tot Spectrometry ISO15 P-tot Spectrometry ISO16 pH pH-meter ISO17 Organic matter Spectrometry ISO18 Dry Matter Gravimetric ISO19 Specific weight Gravimetric ISO20 N-NO3 Spectrometry ISO21 N-NH4 Spectrometry ISO22 P-PO4 Spectrometry ISO23 Oils and grease Gravimetric SMFEWW24 Particle size distribution Hydrometer method MS25 Conductivity Conductmeter ISO26 Sulphate Spectrometry ISO27 Sampling ISO

13 Conductivity14 Salinity Salinity meter Eq. Ins. 15 Turbidity Turbid meter ISO16 TSS Gravimetric ISO17 SO44 Spectrometry ISO18 Ca Volumetric/AAS ISO19 Mg Volumetric/AAS ISO20 TDS Gravimetric ISO21 Permanganate index Volumetric ISO22 Oils and grease Gravimetric SMFEWW23 Coliform total M.F.M SMFEWW24 Fecal Coli forms M.F.M SMFEWW25 Fecal Streptococci M.F.M SMFEWW26 Color Colorimetric SMFEWW27 HCO3 Volumetric SMFEWW28 CaCO3 Volumetric SMFEWW

Page 5: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

No Metals in water

Equipment Methods1 Sampling ISO2 Cr AAS ISO3 Cu AAS ISO4 Pb AAS ISO

No Parameter Equipment Methods1 Sampling ISO2 Digestion Microwave ISO3 Cr AAS ISO4 Cu AAS ISO5 Pb AAS ISO6 Cd AAS ISO

III. Plants AnalysisII.I Water analysis

4 Pb AAS ISO5 Cd AAS ISO6 Ni AAS ISO7 Fe AAS ISO8 Co AAS ISO9 Mn AAS ISO

10 K AAS ISO11 Na AAS ISO12 Ca AAS ISO13 Mg AAS ISO14 Zn AAS ISO

6 Cd AAS ISO7 Ni AAS ISO8 Fe AAS ISO9 Co AAS ISO

10 Mn AAS ISO11 K AAS ISO12 Na AAS ISO13 Ca AAS ISO14 Mg AAS ISO15 Zn AAS ISO16 N-tot Spectrometry ISO17 P-tot Spectrometry ISO18 Dry Matter Gravimetric ISO

Page 6: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

OHRI PROJECT

Page 7: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

Samples

Page 8: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

Samples

Page 9: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

Measure of SRP and TP in extracts

The method is subdivided into two parts;extraction and determination.

TP

Procedure

2 g of sample extracted with 25 ml 0.1M NaOH (shake over 18 hours); determination of reactive phosphorus (SRP) and total phosphorus (TP) in extracts. The determination of SRP & TP is in conformity with ISO 6878:2004 Water quality -- Determination of phosphorus --Ammonium molybdate spectrometric method

SRP

Page 10: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

Phosphorus in sediment Determination of P-total - finally measured as P-PO4Digestion in conformity with EN 16173: August 2012. Sludge, treated biowaste and soil - Digestion of nitric acid soluble fractions of elements

Method

elementsDetermination of P-PO4 in conformity with ISO 6878: 2004- Water quality – Determination of phosphorus –Ammonium molybdatespectrometric method.

Principle: Ammonium molybdate and potassium antimonyl tartrate react in acid medium with orthophosphate to form a phosphomolybdic acid that is reduced to intensely colored molybdenum blue by ascorbic acid.

The absorbance is measure at 880 nm between 10-30 min.

Page 11: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

Nitrogen in sediment• Determination of N-total -finally measured as N- NH4• Digestion in conformity with ISO 11261:1995 Soil quality --Determination of total nitrogen -- Modified Kjeldahl method 2016).

Method

2016). • ISO 11261 specifies a method for the determination of the total nitrogen (ammonium-N, nitrate-N, nitrite-N and organic N) content of a soil. • Determination of N-NH4 in conformity with ISO 7150-1:1984 -Water quality --Determination of ammonium --Part 1: Manual spectrometric

•ISO 7150-1 specifies a spectrometric measurement at about 650 nm of the blue com-pound formed by reaction of ammonium with salicylate and hypochlorite ions in the presence of sodium nitrosopenta-cyanoferrate III (sodium nitroprusside).

Page 12: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

Particle size distribution (Sand, Clay, Silt)

Particle size distribution (Sand, Clay, Silt)

• Hydrometer method The sample is treated with sodium hexametaphosphate to complex Ca++, Al3+, Fe3+, and other cations that bind clay and silt particles into aggregates.

• Organic matter is suspended in this solution. The density of the soil suspension is determined with a hydrometer calibrated to read in grams of solids per liter after the sand settles out and again after the silt settles.

Page 13: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

prEN 15935:2010 (E) Sludge, treated biowaste, soil and waste —Determination of loss on ignition. NOTE: The loss on ignition is often

•Measure of organic matter (O.M)Method

NOTE: The loss on ignition is often used as an estimate for the content of non-volatile organic matter in the sample. It should be noted that inorganic substances or decomposition products (e.g. H2O, CO2, SO2, O2) are released or absorbed and some inorganic substances are volatile under the reaction conditions.

The change in mass as e result of heating a sample under specified conditions.The loss on ignition is expressed as e weight percentage of the dry mass.

Page 14: OHRI PROJECT ppt (2) - WordPress.com · E } D o ] v Á µ ] u v D Z } í^ u o ] v P/^K î ^ /^K ï µ ^ /^K ðW ^/^K E }W u µ ] u v D Z } í^ u o ] v P/^K

Results

CodeO .M (%)

SRP (mg/kg)

TP(mg/kg)

MicrowaveN –Tot

(mg/kg)

Particle size distribution

Sand(%)

Clay (%)

Silt (%)

P (mg/kg)

LO1 Kalishta(2 m) 2.9 4.67 10.01 315.5 1192.3 56.4 7.93 35.7LP1 Stenje 0.99 26.93 30.64 221.0 545.4 80.6 0.0 19.4Ohri bay (2m) 3.9 17.29 25.62 1286.7 1925.4 65.0 0.0 35.0Ohri bay (2m) 3.9 17.29 25.62 1286.7 1925.4 65.0 0.0 35.0Konjosko (2m) 1.2 10.80 17.61 522.3 554.8 88.0 0.0 12.0Tushemisht (2m) 2.3 9.98 19.43 208.0 1682.4 92.2 0.0 7.8LP5 Krani 0.3 16.48 18.58 210.6 132.5 95.9 0.0 4.1LO6 Lin (2m) 2.3 6.26 7.91 636.5 456.8 96.1 3.9 0.0LP3 Galunbah 7.5 2.35 9.61 556.2 3856.9 9.4 12.4 78.2LP6 Sirhan 0.7 19.79 20.52 308.3 227.2 96.0 3.98 0.0LP4 Pustec 5.2 9.52 18.56 552.5 174.5 34.3 8.2 57.5

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