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利用纳米和 eHG干扰技术抑制 6d_J>HG

在 63*V" "/"/$& 细胞中的复制和表达

何艳! 蒋永芳! 王谷丰! 罗红雨! 肖新强! 邓春明! 罗开忠! 苏先狮

!中南大学湘雅二医院肝病研究所" 长沙 [$##$$ #

!摘要"!目的#通过 eHG干扰和纳米技术抑制 6d_J>HG在体外的复制和 6d_核心抗原的表达$

方法#制备靶向 6d_核心抗原"6dBGA#的纳米小干扰 eHG"0@eHG# !利用 8' 启动子质粒转染入 63*V"

"/"/$& 细胞&eFJ=Ye和 i30)3D2 印迹检测转染细胞 6d_核心抗原在 1eHG和蛋白水平的表达情况&D3J

<CJ)@13=Ye检测上清液 6d_J>HG!放射免疫法检测细胞 6d_表面抗原 "6d0GA# %3抗原 "6d3GA# %核心

抗原"6dBGA# $ 结果#成功构建了含磁性纳米的 0@eHG质粒&多种方法检测均显示转染后的细胞 6d_核

心抗原表达明显下降&其表面抗原%3抗原和 6d_J>HG值均较对照组降低$ 结论#eHG干扰联合纳米技

术可明显下调 6d_核心抗原的表达!抑制 6d_J>HG复制$

!关键词"!小干扰 eHG&!6d_&!6d_核心抗原&!磁性纳米

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