The Octave™ Chromatography System is designed for highly productive

puri�cation of chemical and biological compounds. This bench top

8-column system is capable of simulated moving bed chromatography

(SMBC) and other continuous multicolumn protocols that increase

productivity up to 20-fold vs. conventional batch methods. Rather than

�owing �uids sequentially through one static column bed and collecting

fractions as in batch chromatography, SMBC uses a series of interconnected

smaller columns and “moves” the beds in the opposite direction of the �uid

to achieve countercurrent �ow, which greatly facilitates mass transfer and

resolution. Simulated movement of the beds is achieved via multiport valves

that enable periodic switching of the input and output �uids between

adjacent columns in the direction of �uid �ow. All �uid streams are

simultaneously applied and withdrawn at appropriate points between the

columns. When running at a steady state the various stages of separation

are performed simultaneously in di�erent columns in a continuous cycle.

The Octave System overcomes many of the previous limitations of

continuous chromatography, including size and complexity of equipment

and tedious method development. Proprietary valve blocks and control

software automate multicolumn protocols. Intuitive software tools facilitate

conversion of single column data to SMBC parameters. With its small footprint

and modular design, the Octave System brings the high resolution and

e�ciency of SMBC to the bench top, packing more puri�cation capability

into this space than any other similar-size instrument.

• Higher recovery and purity

• Up to 20-fold greater productivity than batch systems

• Up to 90% cost reduction in adsorbents and solvents/bu�ers

• Easy-to-use method development tools and control software

• Robust design with proven performance for many separation chemistries

• Easily customized for speci�c applications

• Available in 3 sizes to obtain grams to kilograms of product per day

ChemistriesA�nity

Chiral

Hydrophobic interaction

Ion exchange

Ion exclusion

Mixed mode

Normal phase

Reversed phase

Size exclusion

TargetsAPIs

Amino acids

Antibiotics

Enantiomers

Fatty acids

Natural products

Oils

Oligosaccharides

Organic acids

Proteins

Sugars

Sugar alcohols

OctaveTM Chromatography SystemContinuous (SMB) chromatography made simple

The power of SMBC on your benchThe power of SMBC on your benchhe power of SMBC on your bench

Modes of operation: Isocratic and StepIsocratic mode is the “classical” SMBC method that utilizes true countercurrent

separation. It is characterized by continuous �ow of a single solvent between

usually 3 or 4 separation zones de�ned by Feed and Desorbent inlets and

Extract (slow moving component) and Ra�nate (fast moving component)

outlets. Isocratic mode is widely used for separating closely related small

molecules such as sugar isomers, enantiomers, and fatty acids.

Step mode is characterized by operation of four or more distinct zones having

di�erent solvent conditions in a bind-elute strategy. Step mode can be used

when the separation factor is large; for example, in a�nity puri�cation of

recombinant proteins and antibodies or fractionating crude preparations by

sequential step elution. A�nity capture e�cency is dramatically enhanced by

having 2 or more columns in the capture zone and loading the �rst column to

near static capacity at high �ow rates; unbound target molecules are captured

by the downstream columns.

Isocratic ModeSeparation of vitamin B12 (1,355 Da; red) and Blue Dextran (2 x 106 Da; blue) by size exclusion on Sephadex® G-25 using eight 5-ml columns in a 3-zone isocratic con�guration on the Octave™ 10 System.

Step ModeProductivities obtained with �ve di�erent Protein A capture resins on the Octave™ 10 System. The 5-step process shown above was used with eight 1-ml columns to purify a human IgG1 mAb from culture �uid at 5 and 7.5 g/L concentrations.

Speci�cations: Octave™ System Dimensions (W / D / H) 21.5 / 21 / 19.5 in (55 / 53 / 50 cm)

Weight 100 lb/45 kg (without pumps)

Power requirements 100–120/220–240 VAC; 2/1 A

Gas inlet supply 330–350 psi (23–24 bar); recommend 99.99% N2

Valve pressure At least 15 psi (1 bar) above �uid pressure

Maximum �uid pressure 270 psi (18.6 bar)

Pumps Four in standard con�guration with maximum of 8; stepper motor dual piston; PEEK™ or stainless steel �ow path; self-�ush pistons; accuracy +/– 3%

Flow rate range 0–12, 0–100, 0–300 ml/min (depending on system)

Valve block 72 pneumatic two-way valves; Ultem® or PCTFE �ow path

Inlets 4 ; each can be shared by 2 pumps

Outlets Maximum 4

Columns Maximum 8

UV-VIS detector Octave™ 4XE UV-VIS Detector available separately; wavelength range 190–840 nm with up to 4 wavelengths measured simultaneously

Ordering Information Maximum Typical OutputCon�guration Cat. No. Pump Speed Column Size Per Day

Octave 10 System H1001K 12 ml/min 1–10 ml 1–30 gOctave 10-HS System H1020K

Octave 100 System H1002K 100 ml/min 10–100 ml Up to 300 gOctave 100-HS System H1014K

Octave 300 II System H1036K 300 ml/min 50–1,000 ml Up to 1 kgOctave 300 II-HS System H1081K

Octave 4XE UV-VIS Detector H1034K

HS systems include PCTFE (rather than Ultem) valve blocks for use with most organic solvents. Users may specify Octave 12-S, 100-S, or 300-S pumps containing stainless steel rather than PEEK (non-metal) �ow paths. Please inquire for custom con�gurations.

Please inquire about Semba’s ProPD™ and ProGMP™ Systems for bioprocess development and single-use manufacturing.

ContactUSA info@sembabio.com +1 866 634 1114

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