Objectives To determine the properties and

quality of the horse joint supplements Legend and Hyalovet by:

•Using the rheometers to measure the viscosity and elasticity.

•Finding the intrinsic viscosity using capillary viscometers.

Rheometers

Rate Control Strain Control

Viscometer Glass tubes that

can be used to calculate the intrinsic viscosity of polymer solutions by measuring the rate at which a liquid falls through the capillary at different dilutions.

Capillary

Timing Lines

Hyaluronic Acid

•Biopolyelectrolyte•High molecular weight•viscoelastic properties•costs 100,000 per kilogram

Sources of HA•rooster combs•micro-organisms•umbilical cords

Intrinsic Viscosities of HA Supplements in PBS

0.00

200.00

400.00

600.00

800.00

1000.00

1200.00

1400.00

1600.00

1800.00

2000.00

0.00E+00 2.00E-04 4.00E-04 6.00E-04 8.00E-04 1.00E-03 1.20E-03

Concentration HA (mg/ml)

(T-T

S)/

(TS

*C)

Trial 1 Hyalovet

Trial 2 Hyalovet

Trial 1 Legend

Trial 2 Legend

Linear (Trial 2Hyalovet)Linear (Trial 1Hyalovet)Linear (Trial 2Legend)Linear (Trial 1Legend)

IV = 534IV = 531(ml/g)

Intrinsic Viscosity = 1074 ml/g

IV = 991 ml/g

Moduli of Legend and Hyalovet

0.00

0.01

0.10

1.00

10.00

0.10 1.00 10.00

Frequency (hz)

Mod

uli (

Pa) Legend Elastic Modulus (Pa)

Legend Viscous Modulus (Pa)

Hyalovet Elastic Modulus (Pa)

Hyalovet Viscous Modulus (Pa)

Viscosity of Legend and Hyalovet

0.01

0.10

1.00

0.10 1.00 10.00 100.00 1000.00

Shear Rate (1/s)

Vis

cosi

ty (

Pas

)

Legend Viscosity

Hyalovet Viscosity

They Came to Tame the Lame

Conclusions The experiments performed on the

rheometer showed similar readings for the flow of Legend and Hyalovet

Legend and Hyalovet showed the same viscous and elastic moduli.

The intrinsic viscosity of Hyalovet is twice that of Legend which shows that Hyalovet has a larger molecular weight.