Nunclon Sphera products for spheroid cultureMaking your most valuable work your best work

Suspension growth

Advancing your cell culture researchOptimizing spheroid formationThe Thermo Scientific™ Nunclon™ Sphera culture surface allows cells to grow in suspension with virtually no cell attachment. The surface supports many different cell types and their ability to generate spheroids; studies show that over time, the spheroids grow in volume, indicating that the new surface enables consistent, repeatable cell growth. 

Enhancing spheroid cultures across many cell types

Expanding size of spheroids with different seeding densities

P19.CL6 spheroid formation over time

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Outperforming nontreated petri dish growth for cancer spheroid culture

Growing 3D cancer culturesThe Nunclon Sphera surface minimizes variability and supports the consistent formation of cancer spheroids that simulate the 3D structures of tumor growth, providing a better model system for studying tumor cell progression and efficacy of anticancer agents in vitro.

HeLa

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Generating a favorable number of cancer spheroids

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Optimizing EB formation of pluripotent stem cells

Elevating your stem cell researchPromoting formation of embryoid bodies (EB)As an important milestone in the stem cell differentiation process, the Nunclon Sphera surface demonstrates superior quality for embryoid body formation of pluripotent stem cells with minimal spontaneous differentiation. The resultant cells are able to differentiate into all three germ layers.

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Nunclon Sphera surface

Non-treated surface

Cell culture–treated surface

Maintaining differentiation potential of pluripotent stem cells

Nunclon Sphera culture

Beta-tubulin (ectoderm) AFP (endoderm) Alpha-SMA (mesoderm)

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Outperforming other surfaces for consistent EB formation of different pluripotent stem cells

Alexa Fluor 488/DAPI, 63x Alexa Fluor 488/DAPI, 63x Invitrogen™ Alexa Fluor™ 488/DAPI, 63x

Alexa Fluor 488/DAPI, 63x

Find out more at thermofisher.com/sphera

For Research Use Only. Not for use in diagnostic procedures. © 2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. COL04796 0917

Ordering information

Product DescriptionUnits per bag

Units per case

Cat. No.

Nunclon Sphera Microplate

96-Well Plate, Round Bottom, 300 μL/well 1 8 174925

96-Well Plate, Flat Bottom, 400 μL/well 1 8 174927

96-Well Plate, Round Bottom, 300 μL/well (bulk pack) 5 50 174929

Nunclon Sphera Multidish

24-Well Plate, 1.9 cm2/well culture area 1 7 174930

12-Well Plate, 3.8 cm2/well culture area 1 7 174931

6-Well Plate, 9.6 cm2/well culture area 1 7 174932

Nunclon Sphera Dish

35 mm, 8.8 cm2 culture area 5 20 174943

60 mm, 21.5 cm2 culture area 5 20 174944

90 mm, 56.7 cm2 culture area 5 20 174945

T25 Nunclon Sphera Flask 25 cm2 culture area 6 18 174951

T75 Nunclon Sphera Flask 75 cm2 culture area 4 24 174952