nucleic acid purification - gene target solutions · purify dna at high yields from yeast the...

Nucleic Acid Purification

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Selection Guide

For PCR only For PCR and other applications

Nucleic Acid Extraction and Purification Products

QuickExtract™ Bacterial DNA Extraction Kit

MasterPure™ Gram-Positive DNA Purification Kit

QuickExtract™ DNA Extraction Solution

BuccalAmp™ DNA Extraction Kit

MasterPure™ Complete DNA and RNA Purification Kit

MasterPure™ DNA Purification Kit

MasterPure™ Yeast DNA Purification Kit

MasterPure™ DNA Purification Kit for Blood Version II

QuickExtract™ Seed DNA Extraction Solution

QuickExtract™ Plant DNA Extraction Solution

MasterPure™ Plant Leaf DNA Purification Kit

QuickExtract™ FFPE DNA Extraction Kit


WaterMaster™ DNA Purification Kit

Meta-G-Nome™ DNA Isolation Kit

ExtractMaster™ Fecal DNA Extraction Kit

SoilMaster™ DNA Extraction Kit

Meta-G-Nome™ DNA Isolation Kit

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Selection Guide

For PCR only For PCR and other applications


MasterPure™ RNA Purification Kit

QuickExtract™ RNA Extraction Kit


MasterPure™ RNA Purification Kit

ArrayPure™ Nano-scale RNA Purification Kit

MasterPure™ Yeast RNA Purification Kit

MasterPure™ Plant RNA Purification Kit

QuickExtract™ FFPE RNA Extraction Kit


QuickExtract™ – Rapid nucleic acid extraction for PCR applications

Rapid, efficient method to extract genomic DNA and RNA from virtually any sample for PCR-based assays. Most samples can be processed with only two short heating steps (65°C and 98°C).

The QuickExtract products provide an inexpensive method for isolating PCR-ready DNA or RT-PCR-ready RNA from one to hundreds of samples, without centrifugation, spin columns, or the use of any toxic organic solvents. The simple, rapid procedure is ideal for high-throughput assays.

MasterPure™–Nucleic acid purification for sequencing and other molecular biology applications

The MasterPure products purify nucleic acids from virtually any sample. Most protocols can be completed in less than one hour and may be scaled up or down as needed. High purity and yields are assured, and kits are free of toxic organic solvents.

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Purification of Genomic DNA

MasterPure™ Complete DNA and RNA Purification KitPurify TNA, DNA, or RNA from any source

The MasterPure Complete DNA and RNA Purification Kit enables efficient purification of intact total nucleic acid (TNA), DNA, or RNA from every type of biological material. The purified DNA is suitable for sequencing and other molecular biology applications.

• Purify DNA in 30 minutes without spin columns.• A260/A280 ratios consistently between 1.8 and 2.0.• Recover >90% of theoretical DNA yield, including both low- and high-molecular-

weight nucleic acids.• No phenol, chloroform, or other caustic solvents.

Cat. # Quantity

MasterPure™ Complete DNA and RNA Purification KitMC89010 5/10 PurificationsMC85200 200 PurificationsFor isolating TNA, DNA, or RNA.Contents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, 2X T&C Lysis Solution, TE Buffer, RNase A, RNase-Free DNase I, Proteinase K, 1X DNase Buffer, RiboGuard RNase Inhibitor.Note: Cat. # MC89010 contains reagents for 10 TNA, 10 DNA, or 5 RNA purifications and Cat. # MC85200 contains reagents for 200 TNA, 200 DNA, or 100 RNA purifications.

Sample Sample Size TNA µg DNA µg RNA µgHeLa/HL60 cells 1 x 106 cells 10-30 3-12 7-15TissuesLiver 5 mg 33-42 5-10 13-25Brain 5 mg 9-13 6-9 4-11Heart 5 mg 6-10 4-7 4-5Kidney 5 mg 10-17 3-8 14-17Thymus 5 mg 15-30 6-12 9-18Blood 200 µl 3-10 3-9Buffy coat 300 µl 40-55 40-55 3-6Mouse tail 0.5 cm 25-30 9-11 E. coli 3.5 x 106 cells 2.5-2.8 1.3-1.6 1.6-1.8S. mutans 1.5 ml 0.9 Yeast* 2.2 x 106 cells 11-18(S. cerevisiae) 1.1 x 107 cells 70-78

*For extraction of yeast DNA, the MasterPure™ Yeast DNA Purification Kit is recommended.

Table 1. Typical yields obtained using the MasterPure™ Complete Kit.

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MasterPure™ DNA Purification Kit for Blood Version IIPurify DNA from whole blood or buffy coat

The MasterPure DNA Purification Kit for Blood Version II can be used to purify genomic DNA from whole blood or buffy coat. With the optimized protocol for DNA, up to 100 µg of DNA can be recovered from 600 µl of buffy coat in less than 30 minutes. The purified DNA can be used for sequencing, PCR, digestion with restriction enzymes, Southern blotting, or other molecular biology applications.

• Purify DNA in 30 minutes without spin columns.• A260/A280 ratios consistently between 1.8 and 2.0.• Recover >90% of theoretical DNA yield, including both low- and high-molecular-

weight nucleic acids.• No phenol, chloroform, or other caustic solvents.• Scalable protocol for large or small samples.

Cat. # Quantity

MasterPure™ DNA Purification Kit for Blood Version IIMB711740 For 40 ml of whole bloodMB711400 For 400 ml of whole bloodContents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, RNase A, TE Buffer.

Starting Material Yield

600 µl Buffy Coat 87-109 µg

200 µl Whole Blood 3-9 µg

0.6 ml Whole Blood 9-27 µg

0.8 ml Whole Blood 12-36 µg

1 ml Whole Blood 15-45 µg





Table 1. Yields of DNA using the MasterPure™ DNA Purification Kit for Blood Version II.

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MasterPure™ Plant Leaf DNA Purification KitPurify DNA from challenging plant-leaf samples

The MasterPure Plant Leaf DNA Purification Kit is designed to purify DNA from 35 to 100 mg of fresh plant-leaf tissue. The purified DNA can be used for sequencing, microsatellite typing, PCR amplification, restriction digestion, Southern blotting, or other molecular biology applications. Plants tested include Arabidopsis, apple, fern, grape, maize, pine, quillwort, sugarcane, sunflower, and tomato.

• Designed for plants high in polyphenolics, like grapevine.• Scalable protocol takes less than 1 hour.• Yields high-molecular-weight DNA.

Cat. # Quantity

MasterPure™ Plant Leaf DNA Purification KitMPP92010 10 PurificationsMPP92100 100 PurificationsContents: Plant DNA Extraction Solution, Cleanup Solution, TE Buffer.

A 10-µl aliquot of each DNA preparation was separated by electrophoresis on a 0.7% agarose gel; the gel was stained with SYBR® Gold and viewed with a Dark Reader™ Transilluminator. Lanes M, 1-kb DNA ladder; lanes 1-4, Pinot noir grape leaf DNA purified with the MasterPure Kit; lanes 5-8, Pinot noir grape leaf DNA purified with Supplier G Kit. HMW DNA, high-molecular-weight DNA.

MasterPure™ Kit

1 2 3 4 M 5 6 7 8 M

HMW DNA —

Supplier G

Figure 1. Comparative yields of DNA isolated with the MasterPure™ Plant Leaf Kit and another supplier’s kit.

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MasterPure™ Yeast DNA Purification KitPurify DNA at high yields from yeast

The MasterPure Yeast DNA Purification Kit enables efficient, high-yield purification of high-molecular-weight DNA from yeast and other fungi. The protocol involves nonenzymatic cell lysis at 65°C, followed by removal of protein by precipitation, and nucleic acid precipitation and resuspension. The protocol can be easily scaled for larger or smaller samples, including single yeast colonies. The recovered nucleic acid can be used directly in most applications, including sequencing, PCR amplification, restriction endonuclease digestion, Southern blotting, and genomic library preparation.

• Higher yields of yeast chromosomal DNA than with other kits (Fig. 1).• Recover DNA from a wide variety of yeast species, including Candida, Saccharomyces,

Pichia, and Schizosaccharomyces, and filamentous fungi such as Aspergillus and Penicillium.

• Purify high-molecular-weight DNA in less than 40 minutes.• No bead-beating, columns, or phenol or other organic extractions.

Cat. # Quantity

MasterPure™ Yeast DNA Purification KitMPY80010 10 PurificationsMPY80200 200 PurificationsContents: Yeast Cell Lysis Solution, MPC Protein Precipitation Reagent, TE Buffer, RNase A.

DNA was quantitated specifically with Hoechst fluorescent dye 33258, which gives minimal fluorescence with RNA. The data represent the average DNA yields determined by fluorometry from two experiments with S. cerevisiae and C. albicans. The MasterPure Kit produced up to 17 times more DNA from C. albicans and 12 times more DNA from S. cerevisiae than other kits.

0 1,000 2,000 3,000 4,000 5,000 6,000 7,000 8,000DNA, nanograms

MasterPure™ Kit – Supplier Q – Supplier F – MasterPure™ Kit –

Supplier Q – Supplier F –

C. albicans

S. cerevisiae

Figure 1. Comparative yields of DNA.

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MasterPure™ Gram-Positive DNA Purification KitPurify genomic DNA from Gram-positive bacteria

The MasterPure Gram-Positive DNA Purification Kit provides all of the reagents needed to purify DNA from Gram-positive bacteria. The purified DNA is suitable for fosmid library construction, PCR, restriction digests, Southern blotting, and other molecular biology applications. The protocol can also be adapted for total RNA purification.

• Scalable method for large sample volumes.• Yields high-quality, high-molecular-weight DNA.• Lysozyme included; no separate purchase required.• Tested on a variety of species.• Can also be used with Gram-negative bacteria.

Cat. # Quantity

MasterPure™ Gram-Positive DNA Purification KitMGP04020 20 ReactionsMGP04100 100 Reactions

Contents: Gram-Positive Cell Lysis Solution, MPC Protein Precipitation Reagent, Ready-Lyse

Lysozyme, Proteinase K, TE Buffer, RNase A.

DNA purified from B. subtilis (ATCC 6051) was separated on a 1% agarose gel and stained with SYBR® Gold. Lane M, kilobase ladder; lane 1, 300 ng of B. subtilis DNA.

12 kb

1 kb

M 1

Figure 1. Electrophoretic analysis of DNA purified using the MasterPure™ Gram-Positive DNA Purification Kit.

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Cat. # Quantity

Meta-G-Nome™ DNA Isolation Kit MGN0910 10 PurificationsContents: Extraction buffer, Filter Wash Buffer, Meta-Lysis Solution (2X), MPC Protein Precipitation Reagent, Proteinase K, Fosmid Control DNA, RNase A, Ready-Lyse Lysozyme Solution, TE Buffer, 0.45-µm cellulose acetate filter membranes, 1.2-µm cellulose acetate filter membranes.

Meta-G-Nome™ DNA Isolation KitPurify metagenomic DNA from water or soil samples

The Meta-G-Nome DNA Isolation Kit is used to isolate DNA from unculturable or difficult-to-culture microbial species present in environmental water or soil samples. The simple extraction procedure involves low-speed centrifugation prior to filtration, and lysis with Ready-Lyse™ Lysozyme and Proteinase K. The kit isolates high-molecular-weight DNA that is randomly sheared (with fragment sizes approximately 40 kb), does not require size selection, and can be used directly for end-repair and construction of fosmid libraries. The kit can also be used to prepare 16S rRNA metagenomic libraries for phylogenetic classification or species-abundance studies.

• Isolated DNA is also suitable for PCR (Fig. 1) or sequencing.• No bead-beating, agarose plugs, pulse-field gel electrophoresis, or size selection.• No phenol/chloroform extractions or CTAB.

M 1 2 3 4 5 6DNA was isolated following the kit protocol, and PCR was performed using 16S rRNA primers using various dilutions of the template DNA. Lane M, Kilobase ladder; lanes 1 and 2, 1 µl and 5 µl of PCR products from 1:10 template dilution; lanes 3 and 4, 1 µl and 5 µl of PCR products from 1:100 template dilution; lane 5, 1 µl of PCR products from undiluted template; lane 6, 1:10 dilution of sample from lane 5.

Figure 1. PCR amplification of soil metagenomic DNA.

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FosmidMAX™ DNA Purification KitBACMAX™ DNA Purification KitPurify high-quality fosmid or BAC DNA

The FosmidMAX and BACMAX DNA Purification Kits were developed for easy, reliable isolation of high-quality fosmid or BAC DNA for many applications, including sequencing, fingerprinting, PCR, and preparation of shotgun libraries.

• No columns or binding resins that reduce yields and shear the DNA.• No toxic organic solvents.• Very low chromosomal DNA background.• Ideal for isolating DNA from single-copy clones, and those induced using

CopyControl™ Vectors.

Fosmid DNA isolated from six different fosmid clones (lanes 1 to 6) at single-copy number was digested with Hind III and analyzed by agarose gel electrophoresis. Lane M, Kilobase ladder.

M 1 2 3 4 5 6 M 1 10

BAC DNA isolated from 10 different clones (lanes 1 to 10) was digested with Hind III and analyzed by agarose gel electrophoresis. Lane M, Kilobase ladder.

Cat. # Quantity

FosmidMAX™ DNA Purification KitFMAX046 1 KitReagents sufficient for 150 x 1.5 ml; 10 x 40 ml; or 5 x 100 ml purifications.Contents: FosmidMAX Solutions 1-4, RiboShredder RNase Blend, TE Buffer.

BACMAX™ DNA Purification KitBMAX044 1 KitReagents sufficient for 150 x 1.5 ml; 30 x 10 ml; 10 x 40 ml; or 5 x 100 ml purifications.Contents: BACMAX Solutions 1-4, RiboShredder RNase Blend, TE Buffer.

Figure 1. Quality of DNA prepared with the FosmidMAX™ Kit.

Figure 2. Quality of DNA prepared with the BACMAX™ Kit.

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Purification of Cloned DNA

Figure 1. Stability of autoinduced BAC and fosmid clones.

BACMAX96™ DNA Purification KitPurify BAC or fosmid DNA in a 96-well format

The BACMAX96 DNA Purification Kit provides easy, reliable isolation of high-quality BAC or fosmid DNA, that is free of contaminating chromosomal DNA, in a 96-well format. The DNA can be used for sequencing, fingerprinting, PCR, and the preparation of shotgun libraries.

• Isolate up to 1 µg of DNA from a single-copy BAC or fosmid clone and more than 2.5 µg from a CopyControl™ BAC clone.

• Purify 384 clones in less than 3 hours without automation.• DNA is free of E. coli genomic DNA and RNA.• Concentrated DNA obtained.

DNA was isolated from seven different pCC1™ BAC clones (A) and eight different pCC2™ fosmid clones (B), from induced (+) and uninduced (–) cultures, using the BACMAX96™ Kit. For each preparation, an 8-μl aliquot was digested with Hind III and analyzed on a 1% agarose gel for 16 hours at 4°C. The identical fingerprints for induced and uninduced cultures show that autoinduction did not cause deletion or rearrangement.

A B

Cat. # Quantity

BACMAX96™ DNA Purification Kit (with consumable plastics)BAC96116 1 KitReagents sufficient for 4 x 96-well plates.Contents: BACMAX96 Solutions 1-3, BACMAX96 RNase Blend, Plamid-Safe ATP-dependent DNase, Plasmid-Safe 10X Buffer, 100 mM ATP Solution, TE Buffer, BAC and Fosmid Autoinduction Solutions, Bacterial Growth Plates, Filter Plates, Collection Plates, Gas Permeable Growth Plate Sealers, Standard Plate Sealers.


Purification of Total RNA

Cat. # Quantity

MasterPure™ RNA Purification KitMCR85102 100 PurificationsContents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, 2X T&C Lysis Solution, TE Buffer, RNase-Free DNase I, Proteinase K, 1X DNase Buffer, RiboGuard RNase Inhibitor.

MasterPure™ RNA Purification KitPurify RNA from any source

The MasterPure RNA Purification Kit enables efficient purification of intact total RNA from a variety of biological materials. The process retains small RNAs that are typically lost when using column-based RNA purification. The purified RNA can be used directly in many molecular biology applications, e.g., RT-PCR, Northern blots, miRNA analysis, or expression profiling with microarrays.

• No spin columns, phenol, chloroform, or other caustic solvents.• Near-quantitative recovery of even small amounts of RNA.

Sample Sample Size RNA Yield (µg)HeLa/HL60 cells 1 x 106 cells 7-15Liver 5 mg 13-25Brain 5 mg 4-11Heart 5 mg 4-5Kidney 5 mg 14-17Thymus 5 mg 9-18Buffy coat 300 µl 3-6E. coli 3.5 x 106 cells 1.6-1.8

Both adherent and suspension cell cultures were purified. A 200-ng aliquot of each RNA preparation was separated by denaturing formaldehyde agarose gel electrophoresis and visualized with SYBR® Gold. Lane M, RNA Marker; lane 1, HeLa cell RNA; lane 2, Ramos cell RNA; lane 3, BDCM cell RNA; lane 4, NIH3T3 cell RNA; lane 5, NRK cell RNA.

M 1 2 3 4 5 M

— 28 S

— 16 S

Table 1. RNA yields obtained using the MasterPure™ RNA Purification Kit.

Figure 1. Quality of RNA purified with the MasterPure™ RNA Purification Kit.

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MasterPure™ Yeast RNA Purification KitPurify RNA from yeast and filamentous fungi

The MasterPure Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces, Pichia) and filamentous fungi. The kit uses a rapid salting-out process to remove contaminating macromolecules. The purified RNA is suitable for cDNA synthesis and microarray gene expression analysis.

• No hot acid phenol, spin columns, or bead-beating.• Faster than spheroplasting.• No extra enzymes or equipment to purchase.• Yields 25-50 µg RNA from 1 ml of mid-log S. cerevisiae.

RNA was extracted from S. cerevisiae using the MasterPure™ Kit and stored at –20°C for 2 months before analysis on an Agilent Technologies 2100 Bioanalyzer. The electropherogram demonstrates the high-purity, intact RNA.

Figure 1. Purity of RNA.

Cat. # Quantity

MasterPure™ Yeast RNA Purification KitMPY03010 10 ReactionsMPY03100 100 ReactionsContents: Extraction Reagent for RNA, MPC Protein Precipitation Reagent, TE Buffer (in 100-reaction kit only), Proteinase K, RNase-Free DNase I, RiboGuard RNase Inhibitor, 10X DNase Buffer, 2X T & C Lysis Solution.



MasterPure™ Plant RNA Purification KitPurify high-quality RNA from plant tissues

The MasterPure Plant RNA Purification Kit enables purification of intact total RNA from plant tissues. The RNA obtained is suitable for many applications, such as RNA-Seq, RT-PCR, Northern blots, miRNA analysis, and expression profiling with microarrays. Unlike other plant RNA purification kits, a DNase I treatment step is included. Plants tested include banana, citrus, cotton, geranium, grape, maize, maple, raspberry, soybean, strawberry, and tomato leaves, alfalfa sprouts, soybean seedlings and seeds, maize seedlings and mature roots, pine needles, and field mustard seeds.

• Optimized technology for plant tissues to remove polyphenols and polysaccharides. • No spin columns that reduce yields; small RNAs are purified effectively. • Scalable protocol takes less than 1 hour. • No phenol, chloroform, or other caustic solvents.

Cat. # Quantity

MasterPure™ Plant RNA Purification KitMPR09010 10 PurificationsMPR09100 100 PurificationsContents: Plant Tissue and Cell Lysis Solution, 2X T and C Lysis Solution, MPC Protein Precipitation Reagent, RNase-Free Water, RNase-Free DNase 1, Proteinase K, RiboGuard RNase Inhibitor, 100 mM DTT, 10X DNase Buffer.

M 1 2 3 4 5 6 7 MPlant tissue RNA was purified using the MasterPure™ Plant RNA Purification Kit, and 500 ng of each type of RNA was separated on a 1% formaldehyde gel and visualized with SYBR® Gold I. Lanes M, RNA ladder; lane 1, maize seedling RNA; lane 2, grape leaf RNA; lane 3, alfalfa sprout RNA; lane 4, strawberry leaf RNA; lane 5, raspberry leaf RNA; lane 6, pine needle RNA; lane 7, maize root RNA.

Figure 1. Purification of intact plant RNA from a variety of plant tissues.

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Rapid Extraction of PCR-Ready DNA

ExtractMaster™ Fecal DNA Extraction KitExtract PCR-ready DNA from fecal samples

The ExtractMaster Fecal DNA Extraction Kit provides all of the reagents necessary to recover PCR-ready DNA from fecal samples. The kit removes small-molecule inhibitors that often coextract with DNA from such samples. The extracted DNA can be used to amplify bacterial, protist, or animal templates for a variety of physiological and pathophysiological research applications.

• Gentle procedure with no toxic solvents or mechanical cell disruption.• Final DNA recovered in a small volume, making downstream analysis easier.

Figure 1. PCR analysis of fecal DNA extracts.

Extracted DNA was amplified using PCR using primers for a eubacterial rRNA gene. Lane M, 100-bp ladder; lane1, human fecal DNA; lane 2, rat fecal DNA; lane 3, rooster fecal DNA; lane 4, no template. The amplicon is 350 bp.

M 1 2 3 4

Figure 2. Identification of rotavirus from infant fecal samples.

Rotavirus dsRNA was isolated using the ExtractMaster™ Kit and used as templates for RT-PCR. Lane M, 100-bp ladder; lanes 1 and 3, RT-PCR products from nucleic acids isolated by phenol-chloroform extraction; lanes 2 and 4, RT-PCR products from nucleic acids isolated using the ExtractMaster Kit. The 1,062-bp amplicon that appears in lanes 2 and 4 corresponds to the full-length vp7 RNA,

M 1 2 3 4

Cat. # Quantity

ExtractMaster™ Fecal DNA Extraction KitFD05005 5 PurificationsFD05025 25 PurificationsContents: Fecal DNA Extraction Buffer, Proteinase K, Fecal Lysis Buffer, Protein Precipitation Reagent, DNA Precipitation Solution, Pellet Wash Solution, TE Buffer, Inhibitor Removal Resin, Spin Columns.


Rapid Extraction of Nucleic Acids

Cat. # Quantity

QuickExtract™ DNA Extraction SolutionQE0905T 5 mlQE09050 50 ml

QuickExtract™ DNA Extraction KitsRapidly prepare PCR-ready DNA from various sample types

QuickExtract products offer a rapid and efficient method for extracting genomic DNA from virtually any sample for PCR-based assays. Most samples can be processed in 8 minutes with only two sequential heating steps.

• Fast—PCR-ready DNA in 3-8 minutes, ideal for high-throughput workflows.• Safe—No organic extraction.• Efficient—No columns, transfers, or sample loss.

M 1 2 3 4 5 6

M 1 2 3 4 5 6 7 M

Figure 1. FailSafe™ PCR amplification of extracted DNA

Figure 2. PCR amplification of FFPE DNA

Buccal cells were extracted using the BuccalAmp™ DNA Extraction Kit, and all other samples with QuickExtract™ DNA Extraction Solution. PCR was performed using primers to amplify the regions indicated: Lanes 1-3, human β-globin (human buccal cells, HeLa cells, and human hair follicle, respectively); lane 4, transgenic mouse GAPDH (mouse tail snip); lane 5, E. coli 16S ribosomal RNA gene (bacteria); lane 6, transgenic SV40 T antigen (mouse tail snip).

DNA was extracted from a slide-mounted, FFPE-preserved human skeletal muscle tissue section with the QuickExtract™ FFPE DNA Extraction Kit. Two microliters of undiluted, extracted DNA was amplified with primers for three different loci: tumor protein 53 (TP53), dystrophin (DMD), and tumor necrosis factor (TNF). The products were separated on a 3% agarose gel and were visualized with SYBR® Gold. Lane M, 100-bp DNA ladder; lane 1, exon 2 of TP53; lane 2, exon 3 of TP53; lane 3, exon 11 of TP53; lane 4, exon 6 of DMD; lane 5, exon 50 of DMD; lane 6, exon 3 of DMD; lane 7, exon 4 of TNF.

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Rapid Extraction of Nucleic Acids

Products Time Applications Samples Tested

QuickExtract™ DNA Extraction Solution(QE0905T, QE09050)

3-8 min Genotyping, genetic studies, identity testing, viral/microbial screening

Hair follicles, feathers (quill-end cells), tissue-culture cells, buccal cells, zebrafish (organs, scales), mouse tail snips

BuccalAmp™ DNA Extraction Kits(BQ0901SCR, BQ0908SCR, BQ0916SCR)

3-8 min Genotyping, genetic studies, identity testing

Buccal cells from human or animal subjects. Catch-All™

Buccal Swabs included.

QuickExtract™ RNA Extraction Kit(QER09015, QER090150)

6 min RT-PCR Cultured cells: human, mouse, rat, E. coli, S. aureus

QuickExtract™ Plant DNA Extraction Solution(QEP80705, QEP70750)

8 min PCR, e.g., GMO testing Arabidopsis, barley, maize, emmer, pepper, rice, spelt, spinach, soybeans, wheat

QuickExtract™ Seed DNA Extraction Solution(QES08095T, QES080950)

8 min PCR, e.g., GMO testing Apple, cotton, sunflower, tomato, barley, maize, oats, rice, rye, wheat

QuickExtract™ Bacterial DNA Extraction Kit(QEB0905T, QEB09050)

15 min PCR, restriction digests, PFGE, optical mapping

Gram-positive: Bacillus subtilisBifidobacterium sppBrevibacterium linensClostridium perfringensLactobacillious plantarumListeria monocytogenesStaphylococcus equorumStreptococcus agalactiaeStreptococcus pyrogenesStreptococcus thermophilus

Gram-negative: E. coliSalmonella entericaSalmonella typhimuriumVibrio gazogenes

QuickExtract™ FFPE DNA Extraction Kit(QEF81805, QEF81050)

62 min Microsatellite, single-nucleotide polymorphisms (SNP), tumor heterogeneity studies, copy number variations (CNV), methylation analysis, short tandem repeats (STR)

Formalin-fixed, paraffin-embedded human tissue samples

QuickExtract™ FFPE RNA Extraction Kit(QFR82805, QFR82050)

32 min RT-PCR Formalin-fixed, paraffin-embedded human tissue samples

For more information, see: www.epicentre.com/quickextract

QuickExtract™ Family of Products



Figure 1. BuccalAmp™ extraction protocol.

DNA was extracted from 18 self-collected samples with the BuccalAmp™ DNA Extraction Kit, and amplified using primers to the β-globin gene. Lane M, 100-bp DNA ladder; lanes 1-18, representative PCR results.

M 1 18

BuccalAmp™ DNA Extraction KitExtract PCR-ready DNA from buccal samples

The BuccalAmp DNA Extraction Kit is a single-tube system for rapid preparation of DNA from human or other mammalian buccal (cheek) swab samples for PCR-based assays, such as human or animal identity testing, and SNP analysis. The kits incorporate the QuickExtract™ DNA Extraction Solution that permits processing of samples using a simplified protocol. The kits provide a choice of foam Catch-All™ Swabs or MasterAmp™

brushes (see next page), and include aliquots of the QuickExtract Solution as individual sample tubes.

• Gentle sample collection using a swab made of soft, porous foam on a flexible plastic handle; alternative to blood draws.

• Reduces risks from sample collection (e.g., needle sticks) and uses no phenol, chloroform, or other toxic solvents.

• Suitable for high-throughput and automated assays.

Figure 2. PCR amplification of extracted DNA.

Rotate swab inQuickExtract DNA Extraction Solution

™Rotate swab on

the inside of cheekHeat at 65ºC for 1 minute

and 98ºC for 2 minutesPCR-ready

DNA

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Buccal Swabs and BrushesEpicentre’s buccal swabs and brushes are designed for safe, gentle buccal cell collection for subsequent DNA extraction and PCR analysis. Both swabs and brushes are provided individually packaged in sterile hard-pack plastic cylinders (hard pack) or as a soft pack.

Cat. # Quantity

BuccalAmp™ DNA Extraction Kits with Catch-All™ Hard Pack Buccal SwabBQ0901SCR 1 Kit (15 Tubes & Swabs)BQ0908SCR 8 Kits (120 Tubes & Swabs)BQ0916SCR 16 Kits (240 Tubes & Swabs)

BuccalAmp™ DNA Extraction Kits with Catch-All™ Soft Pack Buccal SwabBQ0901SSC 1 Kit (15 Tubes & Swabs)BQ0908SSC 8 Kits (120 Tubes & Swabs)BQ0916SSC 16 Kits (240 Tubes & Swabs)

BuccalAmp™ DNA Extraction Kits with MasterAmp™ Brush Hard PackBQ0901SRB 1 Kit (15 Tubes & Brushes)BQ0908SRB 8 Kits (120 Tubes & Brushes)BQ0916SRB 16 Kits (240 Tubes & Brushes)

BuccalAmp™ DNA Extraction Kits with MasterAmp™ Brush Soft PackBQ0901SBS 1 Kit (15 Tubes & Brushes)BQ0908SBS 8 Kits (120 Tubes & Brushes)BQ0916SBS 16 Kits (240 Tubes & Brushes)

Catch-All™ Sample Collection Swab (Hard Pack)QEC091H 100 buccal swabs in hard-pack plastic carrier

Catch-All™ Sample Collection Swab (Soft Pack)QEC89100 100 buccal swabs

MasterAmp™ Buccal Brush (Hard Pack)MB100BR 100 buccal brushes in hard-pack plastic carrier

MasterAmp™ Buccal Brush (Soft Pack)MB100SP 100 buccal brushes

Catch-All™ Sample Collection Swabs are soft foam swabs on soft, flexible plastic handles. Catch-All Swabs provide gentle, safe buccal sample collection, even for infants, and the porous foam on these swabs catches more of the sample than buccal brushes.

MasterAmp™ Buccal Brushes are soft bristle-type buccal brushes.

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nucleic acid purification - gene target solutions · purify dna at high yields from yeast the...

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