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Nucleic Acid Purification PCR Restriction Enzymes Modifying Enzymes Reverse and in vitro Transcription RNase Inhibitors Markers, Ladders, and Primers Quantitation Miscellaneous Nucleic Acid Purification PolyATtract® mRNA Isolation Systems Z5200, Z5210, Z5300, Z5310 PolyATtract® System 1000 Z5400, Z5420 PureYield™ Plasmid Maxiprep System A2391, A2392, A2393, A6732 PureYield™ Plasmid Midiprep System A2490, A2492, A2495, A2496, A6742 PureYield™ Plasmid Miniprep System A1222, A1223, A6812 PureYield™ RNA Midiprep System Z3740, Z3741, Z3372 ReadyAmp™ Genomic DNA Purification System A7710 ReliaPrep™ Blood gDNA Miniprep System A5081, A5082 ReliaPrep™ FFPE gDNA Miniprep System A2351, A2352 ReliaPrep™ FFPE Total RNA Miniprep Sy Z1001, Z1002 ReliaPrep™ gDNA Tissue Miniprep System A2051, A2052 ReliaPrep™ Large Volume HT gDNA System A2751 ReliaPrep™ miRNA Cell and Tissue Purification System Z6210, Z6211, Z6212 ReliaPrep™ RNA Cell Miniprep System, Z6010, Z6011, Z6012 ReliaPrep™ RNA Tissue Minipreps Z6110, Z6111, Z6112 SV 96 Total RNA Isolation System Z3500, Z3505 SV Total RNA Isolation System Z3100, Z3105, Z3382 Wizard® DNA Clean-Up System, 100 preps A7280 Wizard® Genomic DNA Purification Kit A1120, A1125, A1620 Wizard® PCR Preps DNA Purification System A2180, A7170, A7181 Wizard® Plus Maxipreps DNA Purificatio A7270 Wizard® Plus Megapreps DNA Purification System A7300 Wizard® Plus Midipreps DNA Purification System A7640 Wizard® Plus Minipreps DNA Purification System A7100, A7500, A7510 Wizard® Plus SV Minipreps DNA Purification System A1330, A1340, A1460, A1465, A1470, A6762 Wizard® SV 96 Genomic DNA Purification A2370, A2371, A6782 Wizard® SV 96 PCR Clean-Up System A9340, A9341, A9342, A9345, A6792 Wizard® SV 96 Plasmid DNA Purification A2250, A2255 Wizard® SV 9600 Plasmid Purification System A2258 Wizard® SV Gel & PCR Clean-up System A9280, A9281, A9282, A9285, A6752 Wizard® SV Gel & PCR Clean-Up System with x-tracta A9283, A9284 Wizard® SV Genomic DNA Purification System A2360, A2361, A2365, A6772

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Page 1: Nucleic Acid Purificationmultimedia.promega-news.com/promega/3/3/documents/6176.pdfNucleic Acid Purification PCR Restriction Enzymes Modifying Enzymes Reverse and in vitro Transcription

Nucleic Acid Purification

PCR Restriction Enzymes

Modifying Enzymes

Reverse and in vitro Transcription

RNase Inhibitors Markers, Ladders, and Primers

Quantitation Miscellaneous

Nucleic Acid Purification

PolyATtract® mRNA Isolation Systems Z5200, Z5210, Z5300, Z5310

PolyATtract® System 1000 Z5400, Z5420

PureYield™ Plasmid Maxiprep System A2391, A2392, A2393, A6732

PureYield™ Plasmid Midiprep System A2490, A2492, A2495, A2496, A6742

PureYield™ Plasmid Miniprep System A1222, A1223, A6812

PureYield™ RNA Midiprep System Z3740, Z3741, Z3372

ReadyAmp™ Genomic DNA Purification System A7710

ReliaPrep™ Blood gDNA Miniprep System A5081, A5082

ReliaPrep™ FFPE gDNA Miniprep System A2351, A2352

ReliaPrep™ FFPE Total RNA Miniprep Sy Z1001, Z1002

ReliaPrep™ gDNA Tissue Miniprep System A2051, A2052

ReliaPrep™ Large Volume HT gDNA System A2751

ReliaPrep™ miRNA Cell and Tissue Purification System Z6210, Z6211, Z6212

ReliaPrep™ RNA Cell Miniprep System, Z6010, Z6011, Z6012

ReliaPrep™ RNA Tissue Minipreps Z6110, Z6111, Z6112

SV 96 Total RNA Isolation System Z3500, Z3505

SV Total RNA Isolation System Z3100, Z3105, Z3382

Wizard® DNA Clean-Up System, 100 preps A7280

Wizard® Genomic DNA Purification Kit A1120, A1125, A1620

Wizard® PCR Preps DNA Purification System A2180, A7170, A7181

Wizard® Plus Maxipreps DNA Purificatio A7270

Wizard® Plus Megapreps DNA Purification System A7300

Wizard® Plus Midipreps DNA Purification System A7640

Wizard® Plus Minipreps DNA Purification System A7100, A7500, A7510

Wizard® Plus SV Minipreps DNA Purification System A1330, A1340, A1460, A1465, A1470, A6762

Wizard® SV 96 Genomic DNA Purification A2370, A2371, A6782

Wizard® SV 96 PCR Clean-Up System A9340, A9341, A9342, A9345, A6792

Wizard® SV 96 Plasmid DNA Purification A2250, A2255

Wizard® SV 9600 Plasmid Purification System A2258

Wizard® SV Gel & PCR Clean-up System A9280, A9281, A9282, A9285, A6752

Wizard® SV Gel & PCR Clean-Up System with x-tracta A9283, A9284

Wizard® SV Genomic DNA Purification System A2360, A2361, A2365, A6772

Page 2: Nucleic Acid Purificationmultimedia.promega-news.com/promega/3/3/documents/6176.pdfNucleic Acid Purification PCR Restriction Enzymes Modifying Enzymes Reverse and in vitro Transcription

PCR

4 dNTP's U1240, U1330, U1410, U1420

5X PCR Buffer A through D D2301, D2311, D2321, D2331

5X PCR Buffer E through H D2341, D2351, D2361, D2371

dATP, 100mM U1201, U1202, U1205

dCTP, 100mM U1221, U1222, U1225

dGTP, 100mM U1211, U1212, U1215

DNA 5'-End Labelling System U2010

dNTP Mix, 10mM U1511, U1515

dTTP, 100mM U1231, U1232, U1235

dUTP, 100mM U1191

GoTaq® 1-Step RT-qPCR System A6020

GoTaq® 2-Step RT-qPCR System A6010

GoTaq® Colorless Master Mix M7132, M7133

GoTaq® DNA Polymerase, 100u M3001, M3005, M3008

GoTaq® Flexi DNA Polymerase M8291, M8295, M9596, M8297, M8298

GoTaq® G2 Colorless Master Mix M7832, M7833

GoTaq® G2 DNA Polymerase M7841, M7846, M7848

GoTaq® G2 Flexi DNA Polymerase M7801, M7805, M7806, M7808

GoTaq® G2 Green Master Mix M7822, M7823

GoTaq® G2 Hot Start Colorless Master Mix M7432, M7433

GoTaq® G2 Hot Start Green Master Mix M7422, M7423

GoTaq® G2 Hot Start Polymerase M7401, M7405, M7406, M7408

GoTaq® Green Master Mix, 100 Reactions M7122, M7123

GoTaq® Hot Start Colorless Master Mix M5132, M5133

GoTaq® Hot Start Green Master Mix M5122, M5123

GoTaq® Hot Start Polymerase M5001, M5005, M5006, M5008

GoTaq® Long PCR Master Mix M4021

GoTaq® MDx Hot Start Polymerase, 100u D6001, D6006, D6006, D6008

GoTaq® MDx Hot Start Polymerase, Glycerol Free D6201

GoTaq® MDx Hot Start Taq Polymerase D6101

GoTaq® MDx Taq Polymerase D4001, D4005, D4006

GoTaq® MDx Taq Polymerase, Glycerol Free D4101

GoTaq® PCR Core System I 200rxs M7660, M7665

GoTaq® Probe 1-Step RT-qPCR System A6120

GoTaq® Probe 2-Step RT-qPCR System A6110

GoTaq® Probe qPCR Master Mix A6101, A6102

GoTaq® qPCR Master Mix A6001, A6002

PCR Master Mix M7501, M7502, M7505

PCR Nucleotide Mix, 10mM C1141, C1145

PCR Nucleotide Mix, 25mM U1431, U1432

PCR Optimization Kit D2381

Pfu DNA Polymerase, 100u M7741, M7745

pGEM®-T Easy Vector Systems A1360, A1380

pGEM®-T Vector Systems A3600, A3610

Prime-a-Gene® System U1100

Page 3: Nucleic Acid Purificationmultimedia.promega-news.com/promega/3/3/documents/6176.pdfNucleic Acid Purification PCR Restriction Enzymes Modifying Enzymes Reverse and in vitro Transcription

rATP (100mM), 400ul E6011

rATP, 10mM, 0.5ml P1132

rATP, rCTP, rGTP, rUTP each at 10mM P1221

rCTP (100mM), 400ul E6041

rCTP, 10mM, 0.5ml P1142

rCTP, rGTP, rUTP, & rATP (100mM ea) E6000

rGTP (100mM), 400ul E6031

rGTP, 10mM, 0.5ml P1152

Ribo m7G Cap Analog P1711, P1712

rUTP (100mM), 400ul E6021

rUTP, 10mM, 0.5ml P1162

Set of dUTP, dCTP, dGTP, dATP 100mM each U1245, U1335

Restriction Enzymes

AccI R6411, R6415

AgeI, 100u R7251

AluI, 500u R6281

ApaI, 5,000u R6361

BamHI R6021, R6025

BamHI (HC) R4024, R4027

BanI, 200u R6891

BclI, 1,000u R6651

BglI R6071, R6077

BglII R6081, R6085, R6087

Bovine Serum Albumin, Acetylated R3961, R9461

BssHII, GQ R6831, R6835

BstEII, 2,000u R6641

CfoI, 3,000u R6241

ClaI, GQ R6551. R6555

DdeI R6291

DpnI, 200u R6231

DraI, 2,000u R6271

EcoRI R6011, R6017

EcoRI (HC) R4014, R4017

EcoRV R6351, R6355

HaeII, 1,000u R6661

HaeIII R6171, R7175

HhaI, 1,000u R6441

HincII R6031, R6035, R6037

HindIII R6041, R6045

HinfI R6201, R6205

HpaI R6301, R6305

HpaII R6311, R6315

Hsp92I, 500u R7151

Hsp92II, 1,000u R7161

Page 4: Nucleic Acid Purificationmultimedia.promega-news.com/promega/3/3/documents/6176.pdfNucleic Acid Purification PCR Restriction Enzymes Modifying Enzymes Reverse and in vitro Transcription

I-PpoI, 10,000u R7031

KpnI R6341, R6345

MboI, 200u R6711

MluI, GQ, 1,000u R6381

MspI R6401, R6405

NarI, 200u R6861

NciI, 1,000u R7061

NcoI R6513, R6515

NdeI, 500u R6801

NheI, GQ R6501, R6505

NotI (HC) GQ, 1,000u R4434

NotI, GQ R6431, R6435

NruI, 200u R7091

NsiI, 250u R6531

PstI R6111, R6115

PvuI R6321, R6325

PvuII R6331, R6335

RsaI (HC), 5,000u R4374

RsaI, 1,000u R6371

SacI R6061, R6065

SacII, 500u R6221

SalI R6051, R6055

ScaI (HC), 5,000u R4214

ScaI, 1,000u R6211

SgfI, GQ, 250u R7103

SmaI, GQ R6121, R6025

SnaBI R6791, R6795

SpeI, GQ R6591. R6595

SphI R6261, R6265

SspI, GQ, 500u R6601

StuI, 400u R6421

TaqI R6151, R6155

VspI, 500u R6851

XbaI, GQ R6181, R6185

XhoI R6161. R6165

Modifying Enzymes

Alkaline Phosphatase, Calf Intestinal M1821

Alkaline Phosphatase, Calf Intestinal (HC) M2825

DNA Polymerase I M2051, M2055

DNA Polymerase I Large (Klenow) Fragment M2201, M2206

DNA Polymerase I Large (Klenow) Fragment Mini Kit U1300

Exonuclease III M1811, M1815

Klenow fragment, Exonuclease minus, 100u M2181

LigaFast™ Rapid DNA Ligation System M8221, M8225

Page 5: Nucleic Acid Purificationmultimedia.promega-news.com/promega/3/3/documents/6176.pdfNucleic Acid Purification PCR Restriction Enzymes Modifying Enzymes Reverse and in vitro Transcription

Mung Bean Nuclease, 2,000u M4311

RNase A Solution, 4mg/ml, 5ml A7974

RNase H M4281, M4285

RNase ONE™ Ribonuclease M4261, M4265

RQ1 RNase-Free DNase, 1,000u M6101

S1 Nuclease, 10,000u M5761

Single-Stranded DNA Binding Protein M3011

SP6 RNA Polymerase P1081, P1085

SP6 RNA Polymerase (HC), 2,500u P4084

Subcloning Tools Bundle M1060

T3 RNA Polymerase (HC), 2, 500u P4024

T3 RNA Polymerase, 1,000u P2083

T4 DNA Ligase M1801, M1804

T4 DNA Ligase (HC), 500u M1794

T4 DNA Polymerase M4211, M4215

T4 Polynucleotide Kinase M4101, M4103

T4 RNA Ligase, 500u M1051

T7 RNA Polymerase P2075, P2077

T7 RNA Polymerase (HC), 10,000u P4074

Terminal Deoxynucleotidyl Transferase M1871, M1875

TSAP Thermosensitive Alkaline Phosphatase M9910

Reverse and in vitro Transcription

Access RT-PCR System A1250, A1260, A1280

AccessQuick™ RT-PCR System A1701, A1702, A1703

AMV Reverse Transcriptase M5101, M5109

AMV Reverse Transcriptase (HC), 600u M9004

GoScript™ Rev Trans Mix, Oligo dT A2790, A2791

GoScript™ Rev Trans Mix, Random Primers A2800, A2801

GoScript™ Reverse Transcriptase A5003, A5004

GoScript™ Reverse Transcription System A5000, A5001

ImProm-II™ Reverse Transcriptase A3801, A3802, A3803

ImProm-II™ Reverse Transcription System A3800

M-MLV Reverse Transcriptase M1701, M1705

M-MLV Reverse Transcriptase, RNase (H-) M5301

M-MLV RT RNase (H-) Point Mutant M3681, M3682, M3683

Reverse Transcription System A3500

RiboMAX™ Large Scale RNA Production System P1280, P1300

Riboprobe® Systems P1420, P1430, P1440, P1450, P1460

T7 RiboMAX™ Express Large Scale RNA Prod. Sys. P1320

T7 RiboMAX™ Express RNAi System P1700

Universal RiboClone® cDNA Synthesis System C4360

Page 6: Nucleic Acid Purificationmultimedia.promega-news.com/promega/3/3/documents/6176.pdfNucleic Acid Purification PCR Restriction Enzymes Modifying Enzymes Reverse and in vitro Transcription

RNase Inhibitors

Recombinant RNasin® RNase Inhibitor N2511, N2515

RNasin® Plus RNase Inhibitor N2611, N2615

RNasin® RNase Inhibitor N2111, N2115

Markers, Ladders, and Primers

1.2kb Kanamycin Positive Control RNA C1381

100bp DNA Ladder, 250ul (50 lanes) G2101

100bp DNA Step Ladder, 100ug (100 lanes) G6951

10bp DNA Step Ladder, 32.5ug (50 lanes) G4471

1kb DNA Step Ladder, 90ug (300 lanes) G6941

200bp DNA Step Ladder, 100ug (100 lanes) G6961

25bp DNA Step Ladder, 100ug (55 lanes) G4511

50bp DNA Step Ladder, 340ug/ml 90ug (52 lanes) G4521

BenchTop 100bp DNA Ladder, 300ul G8291

BenchTop 1kb DNA Ladder, 600ul G7541

BenchTop PCR Markers, 300ul G7531

BenchTop pGEM® DNA Markers, 250ul G7521

BenchTop PhiX174 DNA/Hae III Markers, 250ul G7511

Blue/Orange Loading Dye, 6X, 3ml (3x1ml) G1881

DNA Ladder, 1kb, 500ul (100 lanes) G5711

EcoR I Adaptors, 150pmole C1291

Herring Sperm DNA, 10mg D1811, D1815, D1816

Human Genomic DNA, 100ug G3041

Human Genomic DNA: Female, 100ug G1521

Human Genomic DNA: Male, 100ug G1471

K562 Genomic DNA, 400ug/mL E4931

Lambda DNA / EcoR I + Hind III Markers, G1731

Lambda DNA / EcoR I Markers, 100ug G1721

Lambda DNA / Hind III Markers, 100ug G1711

Lambda DNA, 250ug D1501

Mouse Genomic DNA, 100ug G3091

Oligo(dT)15 Primer, 500ug/ml 20ug C1101

pBR322 DNA, 10ug D1511

PCR Marker, 250ul (50 lanes) G3161

pGEM® DNA Markers, 50ug (50 lanes) G1741

PhiX174 DNA / Hae III Markers, 50ug G1761

PhiX174 DNA / Hinf I Markers, 50ug G1751

PhiX174, RF DNA, 50ug D1531

ProMega-Markers® Lambda Ladders, 40-60 lanes G3011

pTARGET™ Sequencing Primer (24mer) Q4461

pUC/M13 Primer, Forward (17mer), 2ug Q5391

pUC/M13 Primer, Forward (24mer), 2ug Q5601

pUC/M13 Primer, Reverse (17mer), 2ug Q5401

Page 7: Nucleic Acid Purificationmultimedia.promega-news.com/promega/3/3/documents/6176.pdfNucleic Acid Purification PCR Restriction Enzymes Modifying Enzymes Reverse and in vitro Transcription

pUC/M13 Primer, Reverse (22mer), 2ug Q5421

Random Primers, 20ug C1181

RNA Markers, 50ul G3191

SILVER SEQUENCE™ Staining Reagents Q4132

SP6 Promoter Primer, 2ug Q5011

Unmethylated Lambda DNA, 250ug D1521

Quantitation

QuantiFluor® dsDNA System E2670

QuantiFluor® ONE dsDNA Dye E4891

QuantiFluor® ONE dsDNA System E4870, E4871

QuantiFluor® RNA System E3310

QuantiFluor® ssDNA System E3190

Miscellaneous

Bacterial Strain JM109(DE3), 500ul P9801

Bacterial Strain JM109, 500ul P9751

Bacterial Strain NM522, 500ul P2301

BL21(DE3)pLysS Competent Cells >10x6 cfu L1191

DTT, 100mM (Dithiothreitol) P1171

HB101 Competent Cells, 1ml (5 x 200ul) L2011

JM109 Competent Cells, 1ml L1001

JM109 Competent Cells, 1ml (5 x 200ul) L2001

Nuclease-Free Water P1193, P1195, P1197, P1199

Single Step (KRX) Competent Cells, 20 X L3002

Single-use BL21(DE3)pLysS L1195

Single-use HB101 Competent Cells L2015

Single-use JM109 Competent Cells L2005