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nowthe only diffi cult thingabout spectrophotometryis how to say itUV/Visible spectrophotometers guide

GE Healthcare

1www.gelifesciences.com/spectros

ContentsIntroduction 2

Spectrophotometer selection guide 3

Low volumes made easyNanoVue Spectrophotometer 4

Compact, convenient and fl exibleGeneQuant 1300 5

Options for enzyme kineticsUltrospec 2100 pro 6

High-accuracy, multiple-applicationUltrospec 5300 pro, Ultrospec 6300 pro 7

Basic enzyme kineticsNovaspec Plus, Novaspec III 8

Hand-held cell density meterUltrospec 10 Cell Density Meter 9

System control softwareSWIFT II Applications Software 10

IQ/OQ packages 11

Related products 12

2 www.gelifesciences.com/spectros

IntroductionUV/visible spectrophotometers are a ubiquitous tool in the life sciences because of the wide range of molecules that can be detected and analyzed using this technique. Like many other everyday items, it’s easy to forget how long you have had your spectrophotometer and you might not think about its performance or capabilities until you need to replace it . Questions to consider include:

• What applications are routinely run in your laboratory? Could this change in the near future?

• What range of sample volumes do you need to measure? If this varies, look for options for diff erent cuvette sizes and adaptors

• Do you want to measure multiple samples at the same time? If so, consider options for multiple cell holders

• What level of sensitivity do you need? For low concentration samples, a wider bandwidth (3 to 5 nm) will give more consistent results than a narrow (1 nm) bandwidth instrument

• How many users will there be and what level of experience will they have? Pre-programmed methods and password control of your own methods can be useful features

• How will data be recorded, output or stored?

This guide is an introduction to the range ofspectrophotometers available from GE Healthcare, including new instruments for low sample volumes and new features such as wireless data output. Whether you are performing simple colorimetric assays or conducting the most stringent research, there is an instrument from our range to meet your needs. In addition, we off er service contracts and IQ/OQ certifi cation.

Low sample volumes and ease of use

The NanoVue™ spectrophotometer off ersconvenience and speed using a novel sample-plate mechanism. This facilitates direct andreliable measurements of 0.5 to 5 μl nucleic acid and protein samples, without the need for cuvettes or capillaries.

Routine applications

GE Healthcare off ers a number ofspectrophotometers for widely usedapplications such as DNA and RNA quantitation, protein concentration determination, and celldensity measurements. This includes theUltrospec™ 10, a battery-operated, portable, cell density meter; Novaspec™ III and Plusfor protein determination and/or enzyme kinetics; and GeneQuant™100 and 1300 instruments.

Additional features

For more advanced features, the Ultrospec 2100 and 3100 off er temperature control and cell changer options, whilst the Ultrospec 5300 and 6300 are narrow bandwidth instruments for high precision measurements that are compliant with Pharmacopoeia guidelines.


Spectrophotometer selection guide

Nucleic acid purifi cation DNA/RNAquantitation

PCR/sequencing/ hybridization Primerquantitation and design

Proteindetermination (Bradford,Biuret, BCA etc)

Cell culture OD600

Enzymeactivitykineticsat 340 nm

Fluorescent probequantitation, cDNA probes formicroarrays, PCR probes

Notes

NanoVue Novel samplemechanism, eliminates need for cuvettes and facilitates low volume(0.5 to 5 μl) measurements

Ultrospec 6300 pro andUltrospec 5300 pro

1 nm bandwidthinstrument, wavelength range 190 to 1100 nm. Includes 8-position cell changer. Options fortemperature control.Ultrospec 5300pro PC-controlled. Both arePharmacopeia-compliant

Ultrospec 3100 pro 3 nm bandwidthinstrument, wavelength range 190 to 900 nm with 8-position cell changer and options fortemperature control.

Ultrospec 2100 pro 3 nm bandwidthinstrument, wavelength range 190 to 900 nm with 8-position cell changer and options fortemperature control

GeneQuant 1300 Range of cuvettes andadaptors give fl exible sample volume options from 3 μl to 2 ml

GeneQuant 100

Novaspec Plus Visible spectrophotometer, 330 to 800 nm

Novaspec III Visible spectrophotometer, 330 to 800 nm

Ultrospec 10 Battery-powered, portable cell density meter

Recommend Suitable Not Suitable


NanoVue Spectrophotomer

Ordering information

Product Code number

NanoVue with integrated printer(US & CA only)

28-9244-02

NanoVue with integrated printer(Worldwide except US & CA)

28-9232-15

NanoVue, print via computer(US & CA only)

28-9244-03

NanoVue, print via computer(Worldwide except US & CA)

28-9232-16

NanoVue with wireless connectivity(US & CA only)

28-9244-04

NanoVue with wireless connectivity (Worldwide except US &CA)

28-9232-17

Low volumesmade easy NanoVue spectrophotometer is an easy-to-use instrument designed for the reliable measurement of nucleic acid or protein samples.

• Eliminate the need for cuvettes, capillaries or other sample devices―just drop and read

• Conserve precious samples by assaying low volumes of 0.5 to 5 μl. Samples can also be simply recovered with a pipette if required

• Predefi ned methods for nucleic acid quantitation, including concentration, purity, and theoretical Tm

• Predefi ned methods for protein quantitation, including direct UV, Bradford, BCA, Biuret and Lowry, with the ability to run up to 27 standards

• Intuitive software for ease of use; includes automatic self-calibration on startup

Fig 1. NanoVue spectrophotometer showing drop, measure, and read capability. Pipette samples directly onto the novel sample plate for measurement. The sample plate can then be easily and quickly wiped clean for the next sample measurement.

Accessories

Pathlength Calibration kit 28-9244-05

Replacement sample plate kit 28-9244-06

Spare printer paper 20 rolls 28-9182-26

Printer accessory 28-9182-27


Compact, convenient and fl exibleThe GeneQuant 1300 spectrophotometeris a compact instrument pre-programmed with a range of methodsfor the quantitation of proteins, nucleic acids,and bacterial cell cultures. Absorbanceand concentration measurements can bemade at any wavelength, providingfl exibility for future applications.

• Built-in applications for cell density, nucleic acids, proteins, and enzyme kinetics

• cDNA application software for measuring incorporation of Cy™3 and Cy5, or other dyes into purifi ed microarray hybridization probes and PCR products

• Wavelength range of 190 to 1100 nm, with wavelength scan from 200 to 900 nm performed in less than 5 s with zoom facility, peak identifi cation, and on-peak confi rmation

• Visual inspection of a nucleic acid scan can identify the presence of impurities―especially useful with RNA samples

• Optional integrated printer

• USB cable and print via computer software* supplied as standard. Wireless Bluetooth accessory (optional)

• Three-year lamp warranty

*PVC (Print Via Computer) software runs under Windows™ 2000 orWindows XP™ and enables transfer of data into a PC environment using a USB cable. Data can be printed and saved (in a variety of formats) either manually or automatically. PVC is capable of supporting severalinstruments simultaneously.

The GeneQuant 100 is also available, with built in applications for nucleic acids, proteins and cell density measurements.

GeneQuant 1300


Product Code number

GeneQuant 1300 Classic 28-9182-13

GeneQuant 1300 with printer 28-9182-14

GeneQuant 1300 with Bluetooth 28-9182-15

Bluetooth accessory forGeneQuant 1300

28-9182-25



Product Code number

GeneQuant 100 Classic 28-9182-04

GeneQuant 100 Classic with Printer 28-9182-05



PVC Software for GeneQuant 100 28-9231-88

Fig 2. The GeneQuant 1300 can be used with a wide variety ofcuvettes or capillaries for sample volumes from 3 μl to 2 ml.


Options forenzyme kineticsUltrospec 2100 pro is a simple-to-use instrumentfor nucleic acid measurements and enzymekinetics. It provides stored routines for nucleicacid quantitation and a standard curve routine forprotein determination, in addition to measuringabsorbance and concentration. An 8-positionsample changer is supplied as standard.Accessories such as a temperature control unitoption are also available. The instrument can beupgraded for more sophisticated applications, as well as data storage, with SWIFT II software anda PC.

• Preset routines for DNA, RNA, oligonucleotide quantitation, and purity check

• Combines basic measurement modes with graphical modes: wavelength scan, absorbance changes with time, reaction rate determinations, and standard curves can be displayed and printed

• Store up to 18 user-defi ned methods

• Includes serial and parallel outputs as standard (outputs to a range of printers)

• GLP self-test diagnostics

• IQ/OQ certifi cation test plans

• Available in a choice of colors

Ultrospec 2100 pro


Product Code number

Ultrospec 2100 pro Classic 80-2112-21

Yellow 80-2112-22

Plum 80-2112-27

Apple 80-2112-28

Ultrospec 3100 pro Classic 80-2112-31

Yellow 80-2112-32

Plum 80-2112-33

Apple 80-2112-34

Ultrospec 3100 also available with all benefi ts of Ultrospec 2100, plus high resolution display: ability to store up to 50 user defi ned methods and cDNA application for measuring Cy3 , Cy5 and fl uorescein incorporation into probes and PCR products.

Spreadsheet interface software

Scans of absorbance/wavelength may beconverted to a more conventional graph byusing the spreadsheet interface software to export results directly to Microsoft™ Excel. The results can then be formatted or manipulated as appropriate.

Product Code Number

Spreadsheet interface software (requires serial interface cable)Compatible with Ultrospec 2100 pro andUltrospec 3100 pro. Serial interface cable must be purchased separately.

80-2110-73

RS232C Serial Interface Cable 80-2110-97

Fig 3. Ultrospec 2100 pro with optional PC control, SWIFT II software, on PC support plinth.


High-accuracy,multiple-application The Ultrospec 6300 pro and 5300 pro are highperformance instruments featuring 1 nmbandwidth for precision measurements inanalytical and research laboratories. An 8-position sample changer comes as standard. Accessories such as a sipper option are also available.

• Built-in applications for nucleic acid and protein quantitation, cDNA software (measures Cy3, Cy5, and fl uorescein) for microarray probes and PCR products; enzyme kinetics, wavelength scanning, substrate quantitation, and standard curves

• Wide wavelength range 190 to 1100 nm in 0.1 nm data steps for fl exible applications development

• Wavelength accuracy ± 0.5 nm

• Absorbance range: - 3.000 to 3.000 A, ± 0.5% or ± 0.003 A (at 546 nm), whichever is greater

• Reference beam compensation

• Rapid scan feature

• GLP self-diagnostics

• IQ/OQ certifi cation test plans

• Compatible with 21 CFR Part 11.

• Pharmacopoeia compliant with Qualifi cation and Performance Verifi cation Logbook as standard.

Ultrospec 5300 pro and Ultrospec 6300 pro

Fig 4. Ultrospec 6300 stand-alone instrument with built in methods.Ultropsec 5300 PC-controlled instrument with SWIFT II software supplied.


Ultrospec 5300(PC-controlled instrument)

Ultrospec 6300(stand-alone instrument)

Gold 80-2117-56 80-2117-55

Classic 80-2117-70 80-2117-60

Yellow 80-2117-71 80-2117-61

Plum 80-2117-72 80-2117-62

Apple 80-2117-73 80-2117-63


Novaspec Plus and Novaspec III

Accessories and other items

Product Code Number

Test tube adapters (10, 12, 16 mm) 80-2117-47

Novaspec Plus with heated cell holder Includes: Grafi co PC utility software, serial cable, dust cover.

80-2117-51

Chart recorder interface cable 80-3003-55

Lamp Assembly. Tungsten 80-2115-33

Basic enzymekinetics

Fig 5. Novaspec III (right) and Plus (left) include removable cell/test holders for easy cleaning. The Grafi co software supplied allows downloads to PC.

Novaspec III

A light-weight instrument for measuringabsorbance, % transmission, concentration, and rate. It is easy to use―just select wavelength, set reference and measure sample―making it the instrument of choice for teaching laboratories.

• Large display with easy-to-read characters

• Absorbance, % transmission, concentration, factor, and rate

• Ability to measure two wavelengths simultaneously for kinetics assays

• Self test on startup

• Analog output for connection to chart recorder

• 3 student-friendly experiments plus a tutorial on UV/Vis spectrophotometry are included

Novaspec Plus

A good value instrument for general laboratory use. It includes stored methods for proteinquantitation and enzyme kinetics, plus the basic modes of absorbance, transmittance, OD600, and concentration.

• Kinetics for enzyme studies

• Stored protein methods for Bradford, BCA, Biuret, and Lowry

• Bacterial cell culture measurement at OD600

• “Flash Scan” diode array, for rapid wavelength scans

• Graphical display of wavelength scans, kinetic assays (including slope calculation for rate/ activity studies) and standard curves.

• Option for temperature control (factory fi tted)

• Up to 99 stored methods


Product Code number

Novaspec Plus 80-2117-50

Novaspec Plus with heated cell holder Includes: Grafi co PC utility software,serial cable, dust cover.

80-2117-51

Novaspec III 80-2118-00


Hand-heldcell density meterA small, portable, and dedicated cell density OD600 measurement instrument, the Ultrospec 10measures the density of cells (E. coli, other bacteria, and yeast*) in suspension at 600 nm. This battery- powered, hand-held device can be easily used in incubation cabinets, under anaerobic conditions and in other areas of the laboratory where cells are cultured. Spillages are easily wiped from the smooth surface, and the cell compartment area can be sterilized.

Rechargeable batteries provide nearly one month of cord-free use.

• Battery operated for use in cell culture facilities

• Easy to use, easy to clean, easy to sterilize

• Download results to a PC or printer for convenient data storage and retrieval*(not recommended for use with mammalian cell lines).

Ultrospec 10 Cell Density Meter

Fig 6. Ultrospec 10 is available in four color choices.


Product Code number

Ultrospec 10 Cell Density Meter Classic 80-2116-30

Yellow 80-2116-31

Plum 80-2116-32

Apple 80-2116-33


System controlsoftware21 CFR part 11 compliant SWIFT IIApplications Software

21 CFR part 11 SWIFT II Applications Software provides full FDA compliance in terms of electronic records and signatures*.

• Full password protection, electronic records, and signatures compliant

• Full audit trail

• All the functionality of standard SWIFT II Method software

• Compatible with Ultrospec 2100, 3100, 5300, and 6300

SWIFT IIApplications Software

SWIFT II Applications Software provides applicationmodules for wavelength scanning, reactionkinetics, quantitation, multiwavelength, time drive, fraction analysis, Tm and high-throughput bacterial cell culture work. Results can be exported toMicrosoft Excel.

*Note: as in any 21 CFR part 11 compliant environment, it is incumbent on the end user to have the necessary standard operating procedures (SOPs) and training in their use.

• SWIFT II–METHOD, includes all of the modules on a CD–ROM, supplied as standard with the Ultrospec 5300 pro UV/Visible Spectrophotometer.

• SWIFT II–LAB, is an entry level package and caters for the fundamental applications of scanning, kinetics, and quantitation.

• SWIFT II–SPECIALIST comprises less frequently used applications of multiwavelength, time drive, fraction analysis, cell culture, and Tm in a single package

• Includes an audit trail (data log) facility in each module

• Print preview of results with format customization

• Export to spreadsheet capabilities

SWIFT II Applications Software

Fig 7. Export of a wavelength scan to Microsoft Excel.


Product Code number

21 CFR part 11 compliant SWIFT IIsoftwareIncludes: wavelength scanning, reaction kinetics, time drive, quantitation, multiwavelength, fraction analysis, culture, and Tm. No serial interface cable included.

80-2117-57



Product Code number

SWIFT II-METHODIncludes: wavelength scanning, reaction kinetics, time drive, quantitation, multiwavelength, fraction analysis, culture, and Tm. No serial interface cable included.

80-2108-31

SWIFT II-SPECIALISTIncludes: multiwavelength, fraction analysis,culture, and Tm. No serial interface cable included.

80-2111-91

SWIFT II-LABIncludes: wavelength scanning, reaction kinetics, quantitation. No serial interface lead included.

80-2108-26



Installation Qualifi cation/OperationalQualifi cation (IQ/OQ)

IQ/OQ on Spectrophotometers consists of an IQ/OQDocument (Code 80-2118-62) and the related IQ/OQperformance on-site (Code 28-9137-67). It covers:

• Inspection of goods supplied, Instrument, PC, and software

• Basic operation of installed soft- and hardware

• SWIFT II – SWIFT II CFR Software qualifi cation

• Verifi cation of:

• Wavelength accuracy

• Wavelength repeatability

• Spectral bandwidth

• Absorbance accuracy

• Photometric reproducibility

• Accessories qualifi cation

IQ/OQ Service can be provided for the following instruments: Ultrospec 2100pro, Ultrospec 3100pro, Ultrospec 5300pro, Ultrospec 6300pro.

For other instruments, please contact your local GE Healthcare representative for details.


Product Code number

IQ/OQ performance for Ultrospec 28-9137-70

IQ/OQ Documentation for Ultrospec 80-2118-62

IQ/OQ packages


Related products

Fig 8. illustra tissue and cells genomicPrep Mini Spin Kit.

Fig 9. illustra blood genomicPrep Mini Spin Kit.

Fig 10. illustra plasmidPrep Mini Spin Kit.

Nucleic acid preparation products Pack Size Code Number

illustra RNAspin Mini Kit 20 25-0500-70

50 25-0500-71

250 25-0500-72

20 25-0500-73

illustra tissue and cells genomicPrep Mini Spin Kit 50 28-9042-75

250 28-9042-76

25 28-9042-73

50 28-9042-64

250 28-9042-65

illustra blood genomicPrep Midi Flow Kit 25 28-9042-61

100 28-9042-62

illustra bacteria genomicPrep Mini Spin Kit 50 28-9042-58

250 28-9042-59

illustra plasmidPrep Mini Spin Kit 50 28-9042-69

250 28-9042-70

illustra plasmidPrep Midi Flow Kit 25 28-9042-67

100 28-9042-68

Protein sample preparation products Pack size Code Number Preparation

Disposable PD-10 Desalting columns 30 17-0851-01 Protein desalting and buff er exchange

Ab SpinTrap 50 28-4083-47 Antibody purifi cation and proteinenrichment using Protein G as ligand

Protein A HP SpinTrap 16 28-9031-32 Antibody purifi cation and proteinenrichment using Protein G as ligand

2-D Quant Kit 500assays

80-6483-56 Protein quantitation kit

2-D Clean-Up Kit 50 80-6484-51 Removal of interfering contaminants and concentration of total protein

SDS-PAGE Clean-Up Kit 50 80-6484-70 Removal of interfering contaminants

His SpinTrap 50 28-4013-53 Purifi cation of histidine-tagged proteins

Accessories Pack size Code Number

4-position cell changer (10 - 50 mm pathlength) 1 80-2106-01

Spare 8-position cell changer, 10 mm pathlength 1 80-2108-01

6-position Peltier heated cell changer,10 mm pathlength (requires Temperature Control Unit, see 80-2105-49)

1 80-2106-04

8-position water heated cell changer,10 mm pathlength (requires circulation bath)

1 80-2109-70

2-position manual cell changer, 10 mm pathlength 1 80-2109-04

Sipper, complete with UV quartz cell and all tubing(requires single cell holder 80-2106-05 or 80-2106-13)

1 80-2112-15

Temperature Control Unit 1 80-2105-49

For a complete list of accessories, including cells and cell holders, please go to www.gelifesciences.com/spectros

Fig 11. Ab SpinTrap.

GE, imagination at work, and GE monogram aretrademarks of General Electric Company.

Cy, illustra, and NanoVue are trademarks ofGE Healthcare companies.

All third party trademarks are the property of their respective owners.

© 2008 General Electric Company―All rights reservedFirst published January 2008

All goods and services are sold subject to the termsand conditions of sale of the company withinGE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information.

GE Healthcare Bio-Sciences ABBjörkgatan 30, 751 84 UppsalaSweden

GE Healthcare Europe, GmbHMunzinger Strasse 5, D-79111 FreiburgGermany

GE Healthcare Bio-Sciences Corp.800 Centennial Avenue, P.O. Box 1327Piscataway, NJ 08855-1327USA

GE Healthcare Bio-Sciences KKSanken Bldg., 3-25-1, HyakuninchoShinjuku-ku, Tokyo 169-0073Japan

28-9271-31 AA 01/2008

For further information, please visitwww.gelifesciences.com/contactfor your regional representative.

GE Healthcare LimitedAmersham PlaceLittle Chalfont, BuckinghamshireHP7 9NA, UK

www.gelifesciences.com/spectros

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