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  • Note: Please read kit instructions completely before ...foodsafety.neogen.com/pdf/procedures/8470_pro.pdf · paper towel. 6. Transfer 100 µL conjugate from reagent boat to antibody
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Call 800/234-5333 to order or visit us online at www.neogen.com Product Specifications Format: Sandwich ELISA Range of quantitation: 2.5–25 ppm NFDM Controls provided: 0, 2.5, 5, 10 and 25 ppm NFDM Testing time: 30 minutes Ordering Information 8470 Veratox for Total Milk Allergen Note: Please read kit instructions completely before performing test. Questions? Call 800/234-5333 or 517/372-9200 © Neogen Corporation, 2016. Neogen and Veratox are registered trademarks of Neogen Corporation, Lansing, Michigan, USA. FD901-0716 800-234-5333 (USA /Canada) or 517-372-9200 [email protected] • foodsafety.neogen.com 1. Add 150 µL controls and ex- tracted samples to transfer wells. 2. Transfer 100 µL to the anti- body wells. Incubate for 10 minutes. Mix for 20 seconds by sliding back and forth on a flat surface. 3. Dump liquid from antibody wells. 5. Tap out water on absorbent paper towel. 6. Transfer 100 µL conjugate from reagent boat to antibody wells using 12-channel pipettor. Incubate for 10 minutes. Mix for 20 seconds by sliding back and forth on a flat surface. 7. Repeat steps 3–5 by dumping out the liquid, thoroughly washing the wells and tapping dry. 8. Transfer 100 µL substrate from reagent boat to anti- body wells using 12-channel pipettor. Incubate for 10 minutes. Mix for 20 seconds by sliding back and forth on a flat surface. 9. Transfer 100 µL Red Stop from reagent boat to anti- body wells. Mix. 10. Read results in a microwell reader. 4. Wash wells thoroughly. Repeat wash step wash 10 times times with washing solution. Veratox ® for Total Milk

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Page 1: Note: Please read kit instructions completely before ...foodsafety.neogen.com/pdf/procedures/8470_pro.pdf · paper towel. 6. Transfer 100 µL conjugate from reagent boat to antibody

Call 800/234-5333 to order or visit us online at www.neogen.com

Product SpecificationsFormat: Sandwich ELISARange of quantitation: 2.5–25 ppm NFDMControls provided: 0, 2.5, 5, 10 and 25 ppm NFDMTesting time: 30 minutes

Ordering Information8470 Veratox for Total Milk Allergen

Note: Please read kit instructions completely before performing test.Questions? Call 800/234-5333 or 517/372-9200

© Neogen Corporation, 2016. Neogen and Veratox are registered trademarks of Neogen Corporation, Lansing, Michigan, USA. FD901-0716

800-234-5333 (USA /Canada) or [email protected] • foodsafety.neogen.com

1. Add 150 µL controls and ex-tracted samples to transfer wells.

2. Transfer 100 µL to the anti-body wells. Incubate for 10 minutes. Mix for 20 seconds by sliding back and forth on a flat surface.

3. Dump liquid from antibody wells.

5. Tap out water on absorbent paper towel.

6. Transfer 100 µL conjugate from reagent boat to antibody wells using 12-channel pipettor. Incubate for 10 minutes. Mix for 20 seconds by sliding back and forth on a flat surface.

7. Repeat steps 3–5 by dumping out the liquid, thoroughly washing the wells and tapping dry.

8. Transfer 100 µL substrate from reagent boat to anti-body wells using 12-channel pipettor. Incubate for 10 minutes. Mix for 20 seconds by sliding back and forth on a flat surface.

9. Transfer 100 µL Red Stop from reagent boat to anti-body wells. Mix.

10. Read results in a microwell reader.

4. Wash wells thoroughly. Repeat wash step wash 10 times times with washing solution.

Veratox® for Total Milk

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