northwestern university and the chicago ... - native...
TRANSCRIPT
Alex Seglias and Andrea Kramer
Plant Biology and Conservation
Northwestern University and The Chicago Botanic Garden
National Native Seed Conference 2017
Agriculture
Agriculture
Conservation
Agriculture
Conservation
Restoration
National Center for Genetic Resources
Preservation
• Southwest U.S. – Arizona, Colorado, New Mexico, Utah
• Large-scale restorations
• Limited availability of regional native plant material
• 7 common forb species*
• Widespread throughout the Southwest
• High priority species for use in large-scale restorations
• Little information about dormancy and germination
• Physiological and combinational dormancy
• Orthodox
*Selected from a list of species created by the Colorado Plateau Native Plant Program and collaborators
• Low moisture and temperature (-20°C or below)
• Preservation of seeds for hundreds of years
• Ex situ collections in case of extinction or population destruction
1. Southwestern native forb species will be amenable to seed banking conditions (i.e., drying and storing at sub-zero temperatures)
2. Germination response (proportion and rate) will not differ between non-frozen and frozen seeds
3. Seed bank storage effects will not differ within species
www.fs.fed.us
www.fs.fed.us
Heliomeris multiflorashowy goldeneye
Dieteria canescenshoary tansyaster
Packera multilobatalobeleaf groundsel
www.fs.fed.us
greatbasinnativeplants.com
Penstemon comarrhenusdusty beardtongue
Plantago patagonicawoolly plantain
Sphaeralcea parvifoliasmall-leaf globemallow
Cleome serrulataRocky Mountain beeplant
www.fs.fed.us
• Summer and fall 2015
• 9 populations
• Seeds of Success
protocols
UT CO
AZ NM
Utah Colorado
Arizona
New Mexico
Collect seeds
Clean seeds
Plate seeds
Score germination
Stratification Incubation
Cut tests
Seed dryer
Seed bank
1/2
Collect seeds
Clean seeds
Plate seeds
Score germination
10 weeks (3°C)
8 weeks (3°C)
6 weeks (3, 9°C)
No stratification 4 weeks (3, 6, 9°C)
2 weeks (3, 6, 9°C)
20/10°C 25/15°C
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Incubation
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Stratification Incubation
Cut tests
Seed dryer
Seed bank
1/2
Cox p-value = 0.001All treatments shown
Cox p-value = 0.18All treatments shown
Cox p-value = 0.05
Cox p-value = 0.58All treatments shown
Cox p-value = 0.03All treatments shown
Cox p-value = 0.49All treatments shown
Cox p-value = 0.03All treatments shown
Germination Percentage
TRUE
1. Species are amenable to seed banking
2. Germination does not differ after freezing
FOR MOST SPECIES
3. Effects do not differ within species
DIETERIA CANESCENS SHOWS DIFFERENCE IN
RATE
• All species have orthodox seeds and are amenable to seed banking
• Dormancy and germination largely unchanged following seed bank storage
• Effects were most pronounced during stratification for most species
• Understanding early life stages is critical for restorations now and in the future
Andrea Kramer, Kay Havens, Evelyn WilliamsKramer-Havens LabJessa Finch, Jacob Zeldin, Becky Barak, Olga K.Nora Talkington, Alicia Foxx, Lila LeathermanRachel HosnaPatrick Alexander, Kathy See, Dustin RooksHau Truong and the Rio Mesa CenterFunding:Northwestern Plant Biology and ConservationRobert D. Hevey and Constance M. FillingNative Plant Society of New MexicoBLM & Natural Areas Association
The preceding presentation was delivered at the
This and additional presentations available at http://nativeseed.info
2017 National Native Seed ConferenceWashington, D.C. February 13-16, 2017